The fluorescence spectra and binding reaction of neutral red with nucleic acids have been studied. At pH=4.5, a new method for the determination of trace nucleic acids based on fluorescence quenching measurement was e...The fluorescence spectra and binding reaction of neutral red with nucleic acids have been studied. At pH=4.5, a new method for the determination of trace nucleic acids based on fluorescence quenching measurement was established. When 8.0×10 -6 mol/L NR is employed, the linear ranges of the calibration graphs are 0.08-2.0 μg/mL for calf thymus DNA(ctDNA), 0.12-2.2 μg/mL for fish sperm DNA(fsDNA) and 0.30-1.4 μg/mL for yeast RNA(yRNA), respectively. The corresponding detection limits are 30, 33 and 66 ng/mL, respectively. The real samples were analyzed satisfactorily.展开更多
文摘The fluorescence spectra and binding reaction of neutral red with nucleic acids have been studied. At pH=4.5, a new method for the determination of trace nucleic acids based on fluorescence quenching measurement was established. When 8.0×10 -6 mol/L NR is employed, the linear ranges of the calibration graphs are 0.08-2.0 μg/mL for calf thymus DNA(ctDNA), 0.12-2.2 μg/mL for fish sperm DNA(fsDNA) and 0.30-1.4 μg/mL for yeast RNA(yRNA), respectively. The corresponding detection limits are 30, 33 and 66 ng/mL, respectively. The real samples were analyzed satisfactorily.
