目的:探讨基于磁共振小肠造影对比增强序列的影像组学分析对小肠克罗恩病活动性炎症的诊断效能。方法:回顾性分析了82例末端回肠受累克罗恩病患者的磁共振小肠造影及临床资料,患者以磁共振活动指数(MaRIA)为参照标准分为无-轻度活动组和...目的:探讨基于磁共振小肠造影对比增强序列的影像组学分析对小肠克罗恩病活动性炎症的诊断效能。方法:回顾性分析了82例末端回肠受累克罗恩病患者的磁共振小肠造影及临床资料,患者以磁共振活动指数(MaRIA)为参照标准分为无-轻度活动组和中-重度活动组,使用ITK-SNAP对磁共振小肠造影延迟增强图像上的感兴趣区进行勾画,运用PyRadiomics软件对感兴趣区进行特征提取,之后使用FeAture Explorer Pro将提取的特征按7:3比例随机分为训练集和测试集(训练集58例,31/27=阳性/阴性;测试集24例,13/11=阳性/阴性),并进行特征降维及建模。将建立的影像组学模型应用到测试集中进行验证。最后采用受试者操作特征曲线评估影像组学模型对克罗恩病活动性炎症的诊断效能。结果:从磁共振小肠造影延迟增强图像中共提取出374个特征,经过特征降维、筛选后,最终3个特征被纳入到影像组学模型中,克罗恩病活动性炎症影像组学模型为:1.248×wavelet-HLL_firstorder_Kurtosis+0.717×wavelet-LLL_first order_90Percentile+1.116×wavelet-LLL_glcm_JointAverage。训练集结果显示影像组学模型区分无-轻度活动性及中-重度活动性的曲线下面积(AUC)为0.953(95%CI:0.8949~0.9938),敏感度90.3%,特异度88.9%,测试集的影像组学模型的AUC为0.881(95%CI:0.7109~0.9926),敏感度84.6%,特异度81.2%。结论:基于磁共振小肠造影对比增强图像的影像组学模型对克罗恩病小肠活动性炎症具有较高的诊断效能。展开更多
Objective Magnetoencephalography(MEG),a non-invasive neuroimaging technique,meticulously captures the magnetic fields emanating from brain electrical activity.Compared with MEG based on superconducting quantum interfe...Objective Magnetoencephalography(MEG),a non-invasive neuroimaging technique,meticulously captures the magnetic fields emanating from brain electrical activity.Compared with MEG based on superconducting quantum interference devices(SQUID),MEG based on optically pump magnetometer(OPM)has the advantages of higher sensitivity,better spatial resolution and lower cost.However,most of the current studies are clinical studies,and there is a lack of animal studies on MEG based on OPM technology.Pain,a multifaceted sensory and emotional phenomenon,induces intricate alterations in brain activity,exhibiting notable sex differences.Despite clinical revelations of pain-related neuronal activity through MEG,specific properties remain elusive,and comprehensive laboratory studies on pain-associated brain activity alterations are lacking.The aim of this study was to investigate the effects of inflammatory pain(induced by Complete Freund’s Adjuvant(CFA))on brain activity in a rat model using the MEG technique,to analysis changes in brain activity during pain perception,and to explore sex differences in pain-related MEG signaling.Methods This study utilized adult male and female Sprague-Dawley rats.Inflammatory pain was induced via intraplantar injection of CFA(100μl,50%in saline)in the left hind paw,with control groups receiving saline.Pain behavior was assessed using von Frey filaments at baseline and 1 h post-injection.For MEG recording,anesthetized rats had an OPM positioned on their head within a magnetic shield,undergoing two 15-minute sessions:a 5-minute baseline followed by a 10-minute mechanical stimulation phase.Data analysis included artifact removal and time-frequency analysis of spontaneous brain activity using accumulated spectrograms,generating spectrograms focused on the 4-30 Hz frequency range.Results MEG recordings in anesthetized rats during resting states and hind paw mechanical stimulation were compared,before and after saline/CFA injections.Mechanical stimulation elevated alpha activity in both male and female rats pre-and post-saline/CFA injections.Saline/CFA injections augmented average power in both sexes compared to pre-injection states.Remarkably,female rats exhibited higher average spectral power 1 h after CFA injection than after saline injection during resting states.Furthermore,despite comparable pain thresholds measured by classical pain behavioral tests post-CFA treatment,female rats displayed higher average power than males in the resting state after CFA injection.Conclusion These results imply an enhanced perception of inflammatory pain in female rats compared to their male counterparts.Our study exhibits sex differences in alpha activities following CFA injection,highlighting heightened brain alpha activity in female rats during acute inflammatory pain in the resting state.Our study provides a method for OPM-based MEG recordings to be used to study brain activity in anaesthetized animals.In addition,the findings of this study contribute to a deeper understanding of pain-related neural activity and pain sex differences.展开更多
文摘目的:探讨基于磁共振小肠造影对比增强序列的影像组学分析对小肠克罗恩病活动性炎症的诊断效能。方法:回顾性分析了82例末端回肠受累克罗恩病患者的磁共振小肠造影及临床资料,患者以磁共振活动指数(MaRIA)为参照标准分为无-轻度活动组和中-重度活动组,使用ITK-SNAP对磁共振小肠造影延迟增强图像上的感兴趣区进行勾画,运用PyRadiomics软件对感兴趣区进行特征提取,之后使用FeAture Explorer Pro将提取的特征按7:3比例随机分为训练集和测试集(训练集58例,31/27=阳性/阴性;测试集24例,13/11=阳性/阴性),并进行特征降维及建模。将建立的影像组学模型应用到测试集中进行验证。最后采用受试者操作特征曲线评估影像组学模型对克罗恩病活动性炎症的诊断效能。结果:从磁共振小肠造影延迟增强图像中共提取出374个特征,经过特征降维、筛选后,最终3个特征被纳入到影像组学模型中,克罗恩病活动性炎症影像组学模型为:1.248×wavelet-HLL_firstorder_Kurtosis+0.717×wavelet-LLL_first order_90Percentile+1.116×wavelet-LLL_glcm_JointAverage。训练集结果显示影像组学模型区分无-轻度活动性及中-重度活动性的曲线下面积(AUC)为0.953(95%CI:0.8949~0.9938),敏感度90.3%,特异度88.9%,测试集的影像组学模型的AUC为0.881(95%CI:0.7109~0.9926),敏感度84.6%,特异度81.2%。结论:基于磁共振小肠造影对比增强图像的影像组学模型对克罗恩病小肠活动性炎症具有较高的诊断效能。
文摘Objective Magnetoencephalography(MEG),a non-invasive neuroimaging technique,meticulously captures the magnetic fields emanating from brain electrical activity.Compared with MEG based on superconducting quantum interference devices(SQUID),MEG based on optically pump magnetometer(OPM)has the advantages of higher sensitivity,better spatial resolution and lower cost.However,most of the current studies are clinical studies,and there is a lack of animal studies on MEG based on OPM technology.Pain,a multifaceted sensory and emotional phenomenon,induces intricate alterations in brain activity,exhibiting notable sex differences.Despite clinical revelations of pain-related neuronal activity through MEG,specific properties remain elusive,and comprehensive laboratory studies on pain-associated brain activity alterations are lacking.The aim of this study was to investigate the effects of inflammatory pain(induced by Complete Freund’s Adjuvant(CFA))on brain activity in a rat model using the MEG technique,to analysis changes in brain activity during pain perception,and to explore sex differences in pain-related MEG signaling.Methods This study utilized adult male and female Sprague-Dawley rats.Inflammatory pain was induced via intraplantar injection of CFA(100μl,50%in saline)in the left hind paw,with control groups receiving saline.Pain behavior was assessed using von Frey filaments at baseline and 1 h post-injection.For MEG recording,anesthetized rats had an OPM positioned on their head within a magnetic shield,undergoing two 15-minute sessions:a 5-minute baseline followed by a 10-minute mechanical stimulation phase.Data analysis included artifact removal and time-frequency analysis of spontaneous brain activity using accumulated spectrograms,generating spectrograms focused on the 4-30 Hz frequency range.Results MEG recordings in anesthetized rats during resting states and hind paw mechanical stimulation were compared,before and after saline/CFA injections.Mechanical stimulation elevated alpha activity in both male and female rats pre-and post-saline/CFA injections.Saline/CFA injections augmented average power in both sexes compared to pre-injection states.Remarkably,female rats exhibited higher average spectral power 1 h after CFA injection than after saline injection during resting states.Furthermore,despite comparable pain thresholds measured by classical pain behavioral tests post-CFA treatment,female rats displayed higher average power than males in the resting state after CFA injection.Conclusion These results imply an enhanced perception of inflammatory pain in female rats compared to their male counterparts.Our study exhibits sex differences in alpha activities following CFA injection,highlighting heightened brain alpha activity in female rats during acute inflammatory pain in the resting state.Our study provides a method for OPM-based MEG recordings to be used to study brain activity in anaesthetized animals.In addition,the findings of this study contribute to a deeper understanding of pain-related neural activity and pain sex differences.
