Objective The detection of RNA single nucleotide polymorphism(SNP)is of great importance due to their association with protein expression related to various diseases and drug responses.At present,splintR ligase-assist...Objective The detection of RNA single nucleotide polymorphism(SNP)is of great importance due to their association with protein expression related to various diseases and drug responses.At present,splintR ligase-assisted methods are important approaches for RNA direct detection,but its specificity will be limited when the fidelity of ligases is not ideal.The aim of this study was to create a method to improve the specificity of splintR ligase for RNA detection.Methods In this study,a dualcompetitive-padlock-probe(DCPLP)assay without the need for additional enzymes or reactions is proposed to improve specificity of splintR ligase ligation.To verify the method,we employed dual competitive padlock probe-mediated rolling circle amplification(DCPLP-RCA)to genotype the CYP2C9 gene.Results The specificity was well improved through the competition and strand displacement of dual padlock probe,with an 83.26%reduction in nonspecific signal.By detecting synthetic RNA samples,the method demonstrated a dynamic detection range of 10 pmol/L-1 nmol/L.Furthermore,clinical samples were applied to the method to evaluate its performance,and the genotyping results were consistent with those obtained using the qPCR method.Conclusion This study has successfully established a highly specific direct RNA SNP detection method,and provided a novel avenue for accurate identification of various types of RNAs.展开更多
Deformation characteristics of light weight soil with different EPS (expanded polystyrene) sizes were investigated by consolidation tests.The results show that the confined stress-strain relation curve is in S shape,w...Deformation characteristics of light weight soil with different EPS (expanded polystyrene) sizes were investigated by consolidation tests.The results show that the confined stress-strain relation curve is in S shape,which has a good homologous relation with e-p curve and e-lgp curve,and three types of curves reflect obvious structural characteristics of light weight soil.When cement mixed ratio and EPS volume ratio are the same for different specimens,structural strength decreases with the increase of EPS size,but compressibility indexes basically keep unchanged within the structural strength.The settlement of light weight soil can be divided into instantaneous settlement and primary consolidation settlement.It has no obvious rheology property,and 90% of total consolidation deformation can be finished in 1 min.Settlement-time relation of light weight soil can be predicted by the hyperbolic model.S-lgt curve of light weight soil is not in anti-S shape.It is proved that there is no secondary consolidation section,so consolidation coefficient cannot be obtained by time logarithm method.Structural strength and unit price decrease with the increase of EPS size,but the reducing rate of the structural strength is lower than that of the unit price,so the cost of mixed soil can be reduced by increasing the EPS size.The EPS beads with 3-5 mm in diameter are suggested to be used in the construction process,and the prescription of mixed soil can be optimized.展开更多
A moisture-content based constitutive model was proposed based on the hyperbolic model as an attempt to move towards the implementation of unsaturated soil mechanics into routine geotechnical engineering practice. The...A moisture-content based constitutive model was proposed based on the hyperbolic model as an attempt to move towards the implementation of unsaturated soil mechanics into routine geotechnical engineering practice. The stress-strain behavior of in-situ soil at a depth of 5 m was investigated by conducting undrained triaxial compression tests using the remolded soil samples. The test results show that the stress-strain relationship of unsaturated cohesive soil is still hyperbolic. The values of parameters a and b given in the model decrease with increasing the confining pressure for soil samples with the same moisture content and increase with increasing the moisture content for soil samples under the same confining pressure. The relationships between parameters a, b and moisture content were studied for confining pressures of 100, 150, 200 and 250 kPa. The comparison between the measured and predicted stress-strain curves for an additional group of soil samples, having a moisture content of 25.4%, shows that the proposed moisture content-dependent hyperbolic model provides a good prediction of stress-strain behavior of unsaturated cohesive soil.展开更多
Variable pump driving variable motor(VPDVM) is the future development trend of the hydraulic transmission of an unmanned ground vehicle(UGV).VPDVM is a dual-input single-output nonlinear system with coupling,which is ...Variable pump driving variable motor(VPDVM) is the future development trend of the hydraulic transmission of an unmanned ground vehicle(UGV).VPDVM is a dual-input single-output nonlinear system with coupling,which is difficult to control.High pressure automatic variables bang-bang(HABB) was proposed to achieve the desired motor speed.First,the VPDVM nonlinear mathematic model was introduced,then linearized by feedback linearization theory,and the zero-dynamic stability was proved.The HABB control algorithm was proposed for VPDVM,in which the variable motor was controlled by high pressure automatic variables(HA) and the variable pump was controlled by bang-bang.Finally,simulation of VPDVM controlled by HABB was developed.Simulation results demonstrate the HABB can implement the desired motor speed rapidly and has strong robustness against the variations of desired motor speed,load and pump speed.展开更多
The radiative properties of a gold surface with one-dimensional Gaussian random roughness distribution were obtained with the finite-difference time-domain （FDTD） method and the recursive convolution treatment of th...The radiative properties of a gold surface with one-dimensional Gaussian random roughness distribution were obtained with the finite-difference time-domain （FDTD） method and the recursive convolution treatment of the Drude Model. The bi-directional reflection distribution function （BRDF） for both TM mode and TE mode were obtained and compared with the highly accurate experimental data from the earlier work. The incident wavelength varies from 1.152 μm to 3.392 μm and incident angle is at 300-70°, respectively. The results show that, the predicted values and experimental results are in good agreement. The highly specular peak in the BRDF is reproduced in the numerical simulations, and the increase of the TM mode BRDF is found to be attributed to the effect of a variation in the optical constant at the incident wavelength period.展开更多
文摘Objective The detection of RNA single nucleotide polymorphism(SNP)is of great importance due to their association with protein expression related to various diseases and drug responses.At present,splintR ligase-assisted methods are important approaches for RNA direct detection,but its specificity will be limited when the fidelity of ligases is not ideal.The aim of this study was to create a method to improve the specificity of splintR ligase for RNA detection.Methods In this study,a dualcompetitive-padlock-probe(DCPLP)assay without the need for additional enzymes or reactions is proposed to improve specificity of splintR ligase ligation.To verify the method,we employed dual competitive padlock probe-mediated rolling circle amplification(DCPLP-RCA)to genotype the CYP2C9 gene.Results The specificity was well improved through the competition and strand displacement of dual padlock probe,with an 83.26%reduction in nonspecific signal.By detecting synthetic RNA samples,the method demonstrated a dynamic detection range of 10 pmol/L-1 nmol/L.Furthermore,clinical samples were applied to the method to evaluate its performance,and the genotyping results were consistent with those obtained using the qPCR method.Conclusion This study has successfully established a highly specific direct RNA SNP detection method,and provided a novel avenue for accurate identification of various types of RNAs.
基金Project(2012JQ7013)supported by the Natural Science Foundation of Shaanxi Province,ChinaProject(QN2012025)supported by the Fundamental Research Funds for the Central Universities of ChinaProject(2011BSJJ084)supported by Research Foundation of Northwest A&F University,China
文摘Deformation characteristics of light weight soil with different EPS (expanded polystyrene) sizes were investigated by consolidation tests.The results show that the confined stress-strain relation curve is in S shape,which has a good homologous relation with e-p curve and e-lgp curve,and three types of curves reflect obvious structural characteristics of light weight soil.When cement mixed ratio and EPS volume ratio are the same for different specimens,structural strength decreases with the increase of EPS size,but compressibility indexes basically keep unchanged within the structural strength.The settlement of light weight soil can be divided into instantaneous settlement and primary consolidation settlement.It has no obvious rheology property,and 90% of total consolidation deformation can be finished in 1 min.Settlement-time relation of light weight soil can be predicted by the hyperbolic model.S-lgt curve of light weight soil is not in anti-S shape.It is proved that there is no secondary consolidation section,so consolidation coefficient cannot be obtained by time logarithm method.Structural strength and unit price decrease with the increase of EPS size,but the reducing rate of the structural strength is lower than that of the unit price,so the cost of mixed soil can be reduced by increasing the EPS size.The EPS beads with 3-5 mm in diameter are suggested to be used in the construction process,and the prescription of mixed soil can be optimized.
基金Project(50608038) supported by the National Natural Science Foundation of China
文摘A moisture-content based constitutive model was proposed based on the hyperbolic model as an attempt to move towards the implementation of unsaturated soil mechanics into routine geotechnical engineering practice. The stress-strain behavior of in-situ soil at a depth of 5 m was investigated by conducting undrained triaxial compression tests using the remolded soil samples. The test results show that the stress-strain relationship of unsaturated cohesive soil is still hyperbolic. The values of parameters a and b given in the model decrease with increasing the confining pressure for soil samples with the same moisture content and increase with increasing the moisture content for soil samples under the same confining pressure. The relationships between parameters a, b and moisture content were studied for confining pressures of 100, 150, 200 and 250 kPa. The comparison between the measured and predicted stress-strain curves for an additional group of soil samples, having a moisture content of 25.4%, shows that the proposed moisture content-dependent hyperbolic model provides a good prediction of stress-strain behavior of unsaturated cohesive soil.
基金Project(51375029)supported by the National Natural Science Foundation of ChinaProject(20091102120038)supported by Specialized Research Fund for Doctoral Program of Higher Education of China
文摘Variable pump driving variable motor(VPDVM) is the future development trend of the hydraulic transmission of an unmanned ground vehicle(UGV).VPDVM is a dual-input single-output nonlinear system with coupling,which is difficult to control.High pressure automatic variables bang-bang(HABB) was proposed to achieve the desired motor speed.First,the VPDVM nonlinear mathematic model was introduced,then linearized by feedback linearization theory,and the zero-dynamic stability was proved.The HABB control algorithm was proposed for VPDVM,in which the variable motor was controlled by high pressure automatic variables(HA) and the variable pump was controlled by bang-bang.Finally,simulation of VPDVM controlled by HABB was developed.Simulation results demonstrate the HABB can implement the desired motor speed rapidly and has strong robustness against the variations of desired motor speed,load and pump speed.
基金Project(N110204015) supported by the Fundamental Research Funds for the Central Universities
文摘The radiative properties of a gold surface with one-dimensional Gaussian random roughness distribution were obtained with the finite-difference time-domain （FDTD） method and the recursive convolution treatment of the Drude Model. The bi-directional reflection distribution function （BRDF） for both TM mode and TE mode were obtained and compared with the highly accurate experimental data from the earlier work. The incident wavelength varies from 1.152 μm to 3.392 μm and incident angle is at 300-70°, respectively. The results show that, the predicted values and experimental results are in good agreement. The highly specular peak in the BRDF is reproduced in the numerical simulations, and the increase of the TM mode BRDF is found to be attributed to the effect of a variation in the optical constant at the incident wavelength period.
