Objective Junctophilin-2(JPH2)is an essential structural protein that maintains junctional membrane complexes(JMCs)in cardiomyocytes by tethering the plasma membrane to the sarcoplasmic reticulum,thereby facilitating ...Objective Junctophilin-2(JPH2)is an essential structural protein that maintains junctional membrane complexes(JMCs)in cardiomyocytes by tethering the plasma membrane to the sarcoplasmic reticulum,thereby facilitating excitationcontraction(E-C)coupling.Mutations in JPH2 have been associated with hypertrophic cardiomyopathy(HCM),but the molecular mechanisms governing its membrane-binding properties and the functional relevance of its membrane occupation and recognition nexus(MORN)repeat motifs remain incompletely understood.This study aimed to elucidate the structural basis of JPH2 membrane association and its implications for HCM pathogenesis.Methods A recombinant N-terminal fragment of mouse JPH2(residues 1-440),encompassing the MORN repeats and an adjacent helical region,was purified under near-physiological buffer conditions.X-ray crystallography was employed to determine the structure of the JPH2 MORN-Helix domain.Sequence conservation analysis across species and junctophilin isoforms was performed to assess the evolutionary conservation of key structural features.Functional membrane-binding assays were conducted using liposome co-sedimentation and cell-based localization studies in COS7 and HeLa cells.In addition,site-directed mutagenesis targeting positively charged residues and known HCM-associated mutations,including R347C,was used to evaluate their effects on membrane interaction and subcellular localization.Results The crystal structure of the mouse JPH2 MORN-Helix domain was resolved at 2.6Å,revealing a compact,elongated architecture consisting of multiple tandem MORN motifs arranged in a curved configuration,forming a continuous hydrophobic core stabilized by alternating aromatic residues.A C-terminalα-helix further reinforced structural integrity.Conservation analysis identified the inner groove of the MORN array as a highly conserved surface,suggesting its role as a protein-binding interface.A flexible linker segment enriched in positively charged residues,located adjacent to the MORN motifs,was found to mediate direct electrostatic interactions with negatively charged phospholipid membranes.Functional assays demonstrated that mutation of these basic residues impaired membrane association,while the HCM-linked R347C mutation completely abolished membrane localization in cellular assays,despite preserving the overall MORN-Helix fold in structural modeling.Conclusion This study provides structural insight into the membrane-binding mechanism of the cardiomyocyte-specific protein JPH2,highlighting the dual roles of its MORN-Helix domain in membrane anchoring and protein interactions.The findings clarify the structural basis for membrane targeting via a positively charged linker and demonstrate that disruption of this interaction—such as that caused by the R347C mutation—likely contributes to HCM pathogenesis.These results not only enhance current understanding of JPH2 function in cardiac E-C coupling but also offer a structural framework for future investigations into the assembly and regulation of JMCs in both physiological and disease contexts.展开更多
The interaction of surrounding rock with a support system in deep underground tunnels has attracted extensive interest from researchers.However,the effect of high axial stress on tunnel stability has not been fully co...The interaction of surrounding rock with a support system in deep underground tunnels has attracted extensive interest from researchers.However,the effect of high axial stress on tunnel stability has not been fully considered.In this study,compression tests with and without confining pressure were conducted on solid specimens and hollow cylinder specimens filled with aluminium,lead,and polymethyl methacrylate(PMMA)to investigate the strength,deformation and failure characteristics of circular roadways subjected to high axial stress.The influence of the three-dimensional stress on the surrounding rock supported with different stiffness was studied.The results indicate that the strength and peak strain of hollow cylinders filled with PMMA are higher than those of hollow cylinders filled with aluminium or lead,indicating that flexible retaining is beneficial for roadway stability.The results obtained in this paper can contribute to better understanding the support failure of a buried roadway subjected to high axial stress and thus to analyzing and evaluating roadway stability.展开更多
An attribute recognition model for safe thickness assessment between a concealed karst cave and a tunnel is established based on the attribute mathematic theory.The model can be applied to carrying out risk classifica...An attribute recognition model for safe thickness assessment between a concealed karst cave and a tunnel is established based on the attribute mathematic theory.The model can be applied to carrying out risk classification of the safe thickness between a concealed karst cave and a tunnel and to guarantee construction’s safety in tunnel engineering.Firstly,the assessment indicators and classification standard of safe thickness between a concealed karst cave and a tunnel are studied based on the perturbation method.Then some attribute measurement functions are constructed to compute the attribute measurement of each single index and synthetic attribute measurement.Finally,the identification and classification of risk assessment of safe thickness between a concealed karst cave and a tunnel are recognized by the confidence criterion.The results of two engineering application show that the evaluation results agree well with the site situations in construction.The results provide a good guidance for the tunnel construction.展开更多
To research the loading charactefistc of rocks with different structures cut by helical cutting mechanism （HCM）, three different structures of rock （hard-soft-hard rock, soft-hard rock and soft-hard-soft rock） wer...To research the loading charactefistc of rocks with different structures cut by helical cutting mechanism （HCM）, three different structures of rock （hard-soft-hard rock, soft-hard rock and soft-hard-soft rock） were built. And each type model was further divided into three types when the experiments were carried out. To reduce the errors of cutting load caused by manually configured rock in each test, the cutting load of soft rock was taken as a benchmark, and the differences of the cutting load of the different structures of rocks and the soft rock were used to reflect the cutting load change rules of the HCM. The results indicate that, the cutting load of only the HCM top cutting hard rock is larger than that of only the HCM bottom cutting hard rock for dextral HCM, and the cutting load fluctuation is larger, too. However, when the top and the bottom of the HCM cutting hard rock simultaneously, its cutting load is the largest, but the cutting load fluctuation is the least. And the HCM cutting load increment is increased linearly with the increase of rock compressive strength. The HCM cutting load increment is increased exponentially with the increase of hard rock cutting thickness.展开更多
Longhole caving method was used to mine gently inclined thick orebody step by step in a test stope of tin mine under complex filling body. The problem that the complex filling body around the stope affects the stabili...Longhole caving method was used to mine gently inclined thick orebody step by step in a test stope of tin mine under complex filling body. The problem that the complex filling body around the stope affects the stability of roof thickness, chamber and spacer pillar in actual mining was investigated; meanwhile, the formed goaf during mining is so vulnerable that surrounding rock collapses early. Based on this point, elasticity mechanics and limit span theory were used to study separately the roof thickness and the span limit of goaf formed in mining, and then a reasonable roof thickness of 8 m and goaf span of 14 m are proposed. In addition, the stability of roof thickness, chamber and spacer pillar were investigated and analyzed by using numerical analysis method; meanwhile, the field monitoring on the displacement of caving chamber was conducted. The results show that the maximum compressive stress of surrounding rock is 20 MPa, and the maximum tensile stress is 1.2 MPa, which is less than the ultimate tensile strength of 2.4 MPa. Moreover, plastic zone has little influence on stope stability. In addition, the displacement of 11 mm is also smaller. The displacement monitoring results are consistent with the numerical results. Thus, the roof thickness and span of goaf proposed are safe.展开更多
In order to improve the through-thickness homogeneity and properties of aviation aluminum alloy thick plate.The effect of heating-cooling retrogression and re-ageing on the performance of Al-8Zn-2Mg-2Cu alloy thick pl...In order to improve the through-thickness homogeneity and properties of aviation aluminum alloy thick plate.The effect of heating-cooling retrogression and re-ageing on the performance of Al-8Zn-2Mg-2Cu alloy thick plate was investigated by hardness tests, electrical conductivity tests and transmission electron microscopy(TEM) observation.Results revealed that, during retrogression heating, the fine pre-precipitates in surface layer dissolve more and the undissolved η′ or η phases are more coarsened than that of center layer. During slow cooling after retrogression,precipitates continue coarsening but with a lower rate and the secondary precipitation occurs in both layers. Finer precipitates resulting from the secondary precipitation are more in surface. However, the coarsening and secondary precipitation behaviors are restrained in both layers under quick cooling condition. The electrical conductivity and through-thickness homogeneity of precipitates increases while the hardness decreases with cooling rate decreasing. After the optimized non-isothermal retrogression and re-ageing(NRRA) including air-cooling retrogression, the throughthickness homogeneity which is evaluated by integrated retrogression effects has been improved to 94%. The tensile strength, fracture toughness and exfoliation corrosion grade of Al-8Zn-2Mg-2Cu alloy plate is 619 MPa, 24.7 MPa·m^(1/2)and EB, respectively, which indicates that the non-isothermal retrogression and re-aging(NRRA) could improve the mechanical properties and corrosion resistance with higher through-thickness homogeneity.展开更多
The distribution and magnitude of surface substrates were investigated by finite element method and subsurface stresses of the （FEM）. The models of coating single-layer sprayed-coatings on monolithic configurations ...The distribution and magnitude of surface substrates were investigated by finite element method and subsurface stresses of the （FEM）. The models of coating single-layer sprayed-coatings on monolithic configurations with different thicknesses and elastic modulus ratios of coating to substrate were introduced, and the effects of thickness and elastic modulus ratio on the stresses were addressed. The calculation results show that the coating/substrate interface shear stress obviously decreases with increasing coating thickness, due to the location of the maximum shear stress moving away from the coating/substrate interface. At the same time, the magnitude of von Mises stress also declines in the case of thicker coatings. However, the high elastic modulus ratio results in extremely high maximum shear stress and the severe discontinuity of the von Mises stress curves, which leads to the intensive stress concentration on the coating/substrate interface. So the coating configurations with the larger coating thickness and lower difference of elastic modulus between coating and substrate exhibit excellent resistant performance of rolling contact fatigue （RCF）.展开更多
The effect of fin attachment on the thermal stress reduction of exhaust manifold of an off road diesel engine(Komatsu HD325-6) was investigated.For doing this,coupled thermo-fluid-solid analysis of exhaust manifold of...The effect of fin attachment on the thermal stress reduction of exhaust manifold of an off road diesel engine(Komatsu HD325-6) was investigated.For doing this,coupled thermo-fluid-solid analysis of exhaust manifold of the off road diesel engine was carried out.The thermal analysis,including thermal flow,thermal stress,and the thermal deformation of the manifold was investigated.The flow inside the manifold was simulated and then its properties including velocity,pressure,and temperature were obtained.The flow properties were transferred to the solid model and then the thermal stresses and the thermal deformations of the manifold under different operating conditions were calculated.Finally,based on the predicted thermal stresses and thermal deformations of the manifold body shell,two fin types as well as body shell thickness increase were applied in the critical induced thermal stress area of the manifold to reduce the thermal stress and thermal deformation.The results of the above modifications show that the combined modifications,i.e.the thickness increase and the fin attachment,decrease the thermal stresses by up to 28% and the contribution of the fin attachment in this reduction is much higher compared to the shell thickness increase.展开更多
The through-thickness corrosion inhomogeneity of 7050-T7451 Al alloy thick plate was studied using immersion tests, potentiodynamic polarization, electrochemical impedance spectroscopy(EIS), slow strain rate testing(S...The through-thickness corrosion inhomogeneity of 7050-T7451 Al alloy thick plate was studied using immersion tests, potentiodynamic polarization, electrochemical impedance spectroscopy(EIS), slow strain rate testing(SSRT) technique combined with optical microscopy(OM), scanning electron microscopy(SEM) and transmission electron microscopy(TEM). The results show that the through-thickness corrosion resistance is ranked in the order of T/2>surface>T/4. And the 75 mm-thick 7050 alloy plate presents better corrosion resistance than the 35 mm-thick plate. The results are discussed in terms of the combined effect of recrystallization and cooling rate in quenching. Alloy with lower volume fraction of recrystallization and smaller grain aspect ratio displays better corrosion resistance. The lower corrosion resistance caused by the slower cooling rate results from the higher coverage rate of grain boundary precipitates and larger width of precipitate free zone.展开更多
In order to explore drum instability problems of thinning spinning ultra thin-walled tubes with large diameter-to-hickness ratio, experiments of thinning spinning ultra thin-walled tubes with different clearances betw...In order to explore drum instability problems of thinning spinning ultra thin-walled tubes with large diameter-to-hickness ratio, experiments of thinning spinning ultra thin-walled tubes with different clearances between the mandrel and the tube were carried out. The phenomena of drum instability were analyzed. Drum instability mechanism was studied. The important influence of the mandrel-locked ring on stable thinning spinning was found. Besides, two important parameters, namely drum ratio and drum stiffness, were proposed to characterize the drum instability of spinning ultra thin-walled tubes with large diameter-to-thickness ratio. What's more, numerical simulations were applied to explore the influences of different clearance ratios and diameter-to-thickness ratios on the drum instability. As a result, it is found that the mandrel-locked ring is the key to the stability and precision of spinning; drum ratio can reflect the degree of the deformation of the tubes; drum stiffness is a comprehensive index to measure the influences of the tube's own parameters on the spinning instability; both the clearance ratio and diameter-thickness ratio have significant influences on the drum ratio and drum stiffness.展开更多
A new process of hydroforming with controllable radial pressure was proposed to overcome difficulties in the forming of low plastic materials and large height-to-diameter ratio workpieces. A typical 5A06 aluminum allo...A new process of hydroforming with controllable radial pressure was proposed to overcome difficulties in the forming of low plastic materials and large height-to-diameter ratio workpieces. A typical 5A06 aluminum alloy dome was numerically and experimentally investigated. The reasons for typical defects were analyzed under different radial pressures. Effects of radial pressure on the thickness distribution were discussed and optimal radial pressure was determined. It is shown by numerical simulations and experiment that a cup with a drawing ratio of 2.4 is formed by the new process of hydroforming with controllable radial pressure. It is significantly effective for the forming of low plastic materials and large height-to-diameter ratio workpieees. Two typical thinning points exit along the dome wall. With the radial pressure, thinning is decreased effectively at the two points, the dome forming is achieved and thickness distribution is more uniform.展开更多
As the spring equation is limited to the accuracy of mill stiffness and the linearity of the mill spring curve, the traditional gaugemeter automatic gauge control(GM-AGC) system based on spring equation cannot meet th...As the spring equation is limited to the accuracy of mill stiffness and the linearity of the mill spring curve, the traditional gaugemeter automatic gauge control(GM-AGC) system based on spring equation cannot meet the requirements of practical production. In allusion to this problem, a kind of novel GM-AGC system based on mill stretch characteristic curve was proposed. The error existing in calculating strip thickness by spring equation were analyzed first. And then the mill stretch characteristic curve which could effectively eliminate the influence of mill stiffness was described. The novel GM-AGC system has been applied successfully in a hot strip mill, the application results show that the thickness control precision is improved significantly, with the novel GM-AGC system, over 98.6% of the strip thickness deviation of 3.0 mm class can be controlled within the target tolerances of ±20 μm.展开更多
Although multi-stage incremental sheet forming has always been adopted instead of single-stage forming to form parts with a steep wall angle or to achieve a high forming performance, it is largely dependent on empiric...Although multi-stage incremental sheet forming has always been adopted instead of single-stage forming to form parts with a steep wall angle or to achieve a high forming performance, it is largely dependent on empirical designs. In order to research multi-stage forming further, the effect of forming stages(n) and angle interval between the two adjacent stages(Δα) on thickness distribution was investigated. Firstly, a finite element method(FEM) model of multi-stage incremental forming was established and experimentally verified. Then, based on the proposed simulation model, different strategies were adopted to form a frustum of cone with wall angle of 30° to research the thickness distribution of multi-pass forming. It is proved that the minimum thickness increases largely and the variance of sheet thickness decreases significantly as the value of n grows. Further, with the increase of Δα, the minimum thickness increases initially and then decreases, and the optimal thickness distribution is achieved with Δα of 10°.Additionally, a formula is deduced to estimate the sheet thickness after multi-stage forming and proved to be effective. And the simulation results fit well with the experimental results.展开更多
As one of the advanced and efficient means of joining,the clinching process is capable of joining sheets with different materials or different sheet thicknesses.In this article,a novel modified clinching process,i.e.,...As one of the advanced and efficient means of joining,the clinching process is capable of joining sheets with different materials or different sheet thicknesses.In this article,a novel modified clinching process,i.e.,the dieless clinching process,was executed to join AA6061 aluminum alloy with sheet thicknesses of 1.5,2.0,2.5 and 3.0 mm according to different sheet stack-ups.The geometrical characteristics,microhardness distribution,failure behavior,static strength,absorbed energy and instantaneous stiffness of the novel dieless joint were gotten and investigated.The results indicated that the sheet thickness ratio has a notable effect on the failure behavior and mechanical properties of the novel dieless clinched joint,and a relatively large sheet thickness ratio can improve the joint performance when joining sheets with different sheet thicknesses.展开更多
For contact dominated numerical control(NC) bending process of tube, the effect of friction on bending deformation behaviors should be focused on to achieve precision bending forming. A three dimensional(3D) elastic-p...For contact dominated numerical control(NC) bending process of tube, the effect of friction on bending deformation behaviors should be focused on to achieve precision bending forming. A three dimensional(3D) elastic-plastic finite element(FE) model of NC bending process was established under ABAQUS/Explicit platform, and its reliability was validated by the experiment. Then, numerical study on bending deformation behaviors under different frictions between tube and various dies was explored from multiple aspects such as wrinkling, wall thickness change and cross section deformation. The results show that the large friction of wiper die-tube reduces the wrinkling wave ratio η and cross section deformation degree ΔD and increases the wall thinning degree Δt. The large friction of mandrel-tube causes large η, Δt and ΔD, and the onset of wrinkling near clamp die. The large friction of pressure die-tube reduces Δt and ΔD, and the friction on this interface has little effect on η. The large friction of bending die-tube reduces η and ΔD, and the friction on this interface has little effect on Δt. The reasonable friction coefficients on wiper die-tube, mandrel-tube, pressure die-tube and bending die-tube of 21-6-9(0Cr21Ni6Mn9N) stainless steel tube in NC bending are 0.05-0.15, 0.05-0.15, 0.25-0.35 and 0.25-0.35, respectively. The results can provide a guideline for applying the friction conditions to establish the robust bending environment for stable and precise bending deformation of tube bending.展开更多
The strength, fracture toughness and fatigue crack growth resistance of 7050-T7451 aluminum alloy plate with different thicknesses （35 mm and 160 ram） were investigated by means of optical microscope, scanning elect...The strength, fracture toughness and fatigue crack growth resistance of 7050-T7451 aluminum alloy plate with different thicknesses （35 mm and 160 ram） were investigated by means of optical microscope, scanning electron microscope and transmission electron microscope. The results show that thicker plate has lower strength and fracture toughness but higher fatigue crack growth resistance, by comparison to the thinner plate. The drop of strength is mainly attributed to grain coarsening in the thicker plate, and the increased degree of recrystallization results in the loss of Kio However, the coarsened grains in the thicker plate make cracks deflected and closure effect enhanced due to surface roughness increased. For both of plates, in the fracture surface subjected plain strain, a transition from transgranular dimpled fracture to intergranular dimpled fracture is observed during the fracture process.展开更多
Creep ageing forming(CAF)has been widely used in the aerospace engineering,but how to optimize the processing conditions,especially under complex stress state of the CAF process for large-size components produced by f...Creep ageing forming(CAF)has been widely used in the aerospace engineering,but how to optimize the processing conditions,especially under complex stress state of the CAF process for large-size components produced by friction-stir welding is still a great challenge to now.In this work,the creep ageing behaviors and underlying microstructure evolution of a thick friction-stir welded Al-Cu alloy plate after CAF process under different stress levels are systematically investigated.The creep strain and the strength of the joint are both significantly increased when the stress is close to the average yield strength of the initial weld joint.The grain size reduces while the local strain and dislocation density increase from top to bottom of the NZ;hence,the bottom layer of the weld joint exhibits higher creep strain and steady-stage creep strain rate during the CAF process.The results reveal that the gradient microstructures sensitive to the stress level effectively govern the creep-ageing performance from the upper to the bottom layer in a thick friction stir welded Al-Cu alloy plate.Rationally increasing the initial dislocation density of the weld joint can both enhance the tensile properties and promote the creep deformation of the weld joint for CAF process.展开更多
Finite element method was performed to investigate the influences of beam stiffness, foundation width and cushion thickness on the beating capacity of beam foundation on underlying weak laminated clay. The comparison ...Finite element method was performed to investigate the influences of beam stiffness, foundation width and cushion thickness on the beating capacity of beam foundation on underlying weak laminated clay. The comparison between numerical results and results from field test including plate-bearing test and foundation settlement observation shows reasonable agreement. According to the numerical results, the beam width, length, cross section and cushion thickness were optimized. The results show that the stresses in subgrade soil decrease greatly with increasing the cushion thickness and width of foundation. However, the foundation settlement and influencing depth of displacement also increase correspondingly under conditions of relatively thinner cushion thickness. For the foundations on underlying weak layer, increasing foundation width merely might be inadequate for improving the bearing capacity, and the appropriate width and cushion thickness depend on the response of subgrade. A comparison between rigid and flexible beams was also discussed. The influence of a flexible beam foundation on subgrade is relatively smaller under the same loading conditions, and the flexible beam foundation appears more adaptable to various subgrades. The proposed flexible beam foundation was adopted in engineering. According to the calculation results, beam width of 2.4 m and cushion thickness of 0.8 m are proposed, and a flexible beam foundation is applied in the optimized design, which is confirmed reasonable by the actual engineering.展开更多
文摘Objective Junctophilin-2(JPH2)is an essential structural protein that maintains junctional membrane complexes(JMCs)in cardiomyocytes by tethering the plasma membrane to the sarcoplasmic reticulum,thereby facilitating excitationcontraction(E-C)coupling.Mutations in JPH2 have been associated with hypertrophic cardiomyopathy(HCM),but the molecular mechanisms governing its membrane-binding properties and the functional relevance of its membrane occupation and recognition nexus(MORN)repeat motifs remain incompletely understood.This study aimed to elucidate the structural basis of JPH2 membrane association and its implications for HCM pathogenesis.Methods A recombinant N-terminal fragment of mouse JPH2(residues 1-440),encompassing the MORN repeats and an adjacent helical region,was purified under near-physiological buffer conditions.X-ray crystallography was employed to determine the structure of the JPH2 MORN-Helix domain.Sequence conservation analysis across species and junctophilin isoforms was performed to assess the evolutionary conservation of key structural features.Functional membrane-binding assays were conducted using liposome co-sedimentation and cell-based localization studies in COS7 and HeLa cells.In addition,site-directed mutagenesis targeting positively charged residues and known HCM-associated mutations,including R347C,was used to evaluate their effects on membrane interaction and subcellular localization.Results The crystal structure of the mouse JPH2 MORN-Helix domain was resolved at 2.6Å,revealing a compact,elongated architecture consisting of multiple tandem MORN motifs arranged in a curved configuration,forming a continuous hydrophobic core stabilized by alternating aromatic residues.A C-terminalα-helix further reinforced structural integrity.Conservation analysis identified the inner groove of the MORN array as a highly conserved surface,suggesting its role as a protein-binding interface.A flexible linker segment enriched in positively charged residues,located adjacent to the MORN motifs,was found to mediate direct electrostatic interactions with negatively charged phospholipid membranes.Functional assays demonstrated that mutation of these basic residues impaired membrane association,while the HCM-linked R347C mutation completely abolished membrane localization in cellular assays,despite preserving the overall MORN-Helix fold in structural modeling.Conclusion This study provides structural insight into the membrane-binding mechanism of the cardiomyocyte-specific protein JPH2,highlighting the dual roles of its MORN-Helix domain in membrane anchoring and protein interactions.The findings clarify the structural basis for membrane targeting via a positively charged linker and demonstrate that disruption of this interaction—such as that caused by the R347C mutation—likely contributes to HCM pathogenesis.These results not only enhance current understanding of JPH2 function in cardiac E-C coupling but also offer a structural framework for future investigations into the assembly and regulation of JMCs in both physiological and disease contexts.
基金Projects(11772357,51474103,51504092)supported by the National Natural Science Foundation of ChinaProject(2016YFC0600706)supported by the National Key Research and Development Program of China
文摘The interaction of surrounding rock with a support system in deep underground tunnels has attracted extensive interest from researchers.However,the effect of high axial stress on tunnel stability has not been fully considered.In this study,compression tests with and without confining pressure were conducted on solid specimens and hollow cylinder specimens filled with aluminium,lead,and polymethyl methacrylate(PMMA)to investigate the strength,deformation and failure characteristics of circular roadways subjected to high axial stress.The influence of the three-dimensional stress on the surrounding rock supported with different stiffness was studied.The results indicate that the strength and peak strain of hollow cylinders filled with PMMA are higher than those of hollow cylinders filled with aluminium or lead,indicating that flexible retaining is beneficial for roadway stability.The results obtained in this paper can contribute to better understanding the support failure of a buried roadway subjected to high axial stress and thus to analyzing and evaluating roadway stability.
基金Projects(51509147,51879153) supported by the National Natural Science Foundation of ChinaProjects(2017JC002,2017JC001) supported by the Fundamental Research Funds of Shandong University,China
文摘An attribute recognition model for safe thickness assessment between a concealed karst cave and a tunnel is established based on the attribute mathematic theory.The model can be applied to carrying out risk classification of the safe thickness between a concealed karst cave and a tunnel and to guarantee construction’s safety in tunnel engineering.Firstly,the assessment indicators and classification standard of safe thickness between a concealed karst cave and a tunnel are studied based on the perturbation method.Then some attribute measurement functions are constructed to compute the attribute measurement of each single index and synthetic attribute measurement.Finally,the identification and classification of risk assessment of safe thickness between a concealed karst cave and a tunnel are recognized by the confidence criterion.The results of two engineering application show that the evaluation results agree well with the site situations in construction.The results provide a good guidance for the tunnel construction.
基金Project(51005232) supported by the National Natural Science Foundation of ChinaProject(20100481176) supported by the China Postdoctoral Science Foundation
文摘To research the loading charactefistc of rocks with different structures cut by helical cutting mechanism （HCM）, three different structures of rock （hard-soft-hard rock, soft-hard rock and soft-hard-soft rock） were built. And each type model was further divided into three types when the experiments were carried out. To reduce the errors of cutting load caused by manually configured rock in each test, the cutting load of soft rock was taken as a benchmark, and the differences of the cutting load of the different structures of rocks and the soft rock were used to reflect the cutting load change rules of the HCM. The results indicate that, the cutting load of only the HCM top cutting hard rock is larger than that of only the HCM bottom cutting hard rock for dextral HCM, and the cutting load fluctuation is larger, too. However, when the top and the bottom of the HCM cutting hard rock simultaneously, its cutting load is the largest, but the cutting load fluctuation is the least. And the HCM cutting load increment is increased linearly with the increase of rock compressive strength. The HCM cutting load increment is increased exponentially with the increase of hard rock cutting thickness.
基金Project(2012BAK09B02-05)supported by the National Science and Technology Pillar Program during the 12th Five-Year Plan PeriodProject(11KF02)supported by the Research Fund of the State Key Laboratory of Coal Resources and Mine Safety
文摘Longhole caving method was used to mine gently inclined thick orebody step by step in a test stope of tin mine under complex filling body. The problem that the complex filling body around the stope affects the stability of roof thickness, chamber and spacer pillar in actual mining was investigated; meanwhile, the formed goaf during mining is so vulnerable that surrounding rock collapses early. Based on this point, elasticity mechanics and limit span theory were used to study separately the roof thickness and the span limit of goaf formed in mining, and then a reasonable roof thickness of 8 m and goaf span of 14 m are proposed. In addition, the stability of roof thickness, chamber and spacer pillar were investigated and analyzed by using numerical analysis method; meanwhile, the field monitoring on the displacement of caving chamber was conducted. The results show that the maximum compressive stress of surrounding rock is 20 MPa, and the maximum tensile stress is 1.2 MPa, which is less than the ultimate tensile strength of 2.4 MPa. Moreover, plastic zone has little influence on stope stability. In addition, the displacement of 11 mm is also smaller. The displacement monitoring results are consistent with the numerical results. Thus, the roof thickness and span of goaf proposed are safe.
基金Project(51801082) supported by National Natural Science Foundation of ChinaProjects(GY2021003, GY2021020)supported by the Key Research and Development Program of Zhenjiang City,China+1 种基金Project(KYCX21_3453) supported by Graduate Research and Innovation Projects in Jiangsu Province,ChinaProject(202110289002Z) supported by Undergraduate Innovation and Entrepreneurship Training Program of Jiangsu Province,China。
文摘In order to improve the through-thickness homogeneity and properties of aviation aluminum alloy thick plate.The effect of heating-cooling retrogression and re-ageing on the performance of Al-8Zn-2Mg-2Cu alloy thick plate was investigated by hardness tests, electrical conductivity tests and transmission electron microscopy(TEM) observation.Results revealed that, during retrogression heating, the fine pre-precipitates in surface layer dissolve more and the undissolved η′ or η phases are more coarsened than that of center layer. During slow cooling after retrogression,precipitates continue coarsening but with a lower rate and the secondary precipitation occurs in both layers. Finer precipitates resulting from the secondary precipitation are more in surface. However, the coarsening and secondary precipitation behaviors are restrained in both layers under quick cooling condition. The electrical conductivity and through-thickness homogeneity of precipitates increases while the hardness decreases with cooling rate decreasing. After the optimized non-isothermal retrogression and re-ageing(NRRA) including air-cooling retrogression, the throughthickness homogeneity which is evaluated by integrated retrogression effects has been improved to 94%. The tensile strength, fracture toughness and exfoliation corrosion grade of Al-8Zn-2Mg-2Cu alloy plate is 619 MPa, 24.7 MPa·m^(1/2)and EB, respectively, which indicates that the non-isothermal retrogression and re-aging(NRRA) could improve the mechanical properties and corrosion resistance with higher through-thickness homogeneity.
基金Project(2007AA04Z408) supported by the National High-Tech Research and Development Program of ChinaProject(50735006) supported by the National Natural Science Foundation of China
文摘The distribution and magnitude of surface substrates were investigated by finite element method and subsurface stresses of the （FEM）. The models of coating single-layer sprayed-coatings on monolithic configurations with different thicknesses and elastic modulus ratios of coating to substrate were introduced, and the effects of thickness and elastic modulus ratio on the stresses were addressed. The calculation results show that the coating/substrate interface shear stress obviously decreases with increasing coating thickness, due to the location of the maximum shear stress moving away from the coating/substrate interface. At the same time, the magnitude of von Mises stress also declines in the case of thicker coatings. However, the high elastic modulus ratio results in extremely high maximum shear stress and the severe discontinuity of the von Mises stress curves, which leads to the intensive stress concentration on the coating/substrate interface. So the coating configurations with the larger coating thickness and lower difference of elastic modulus between coating and substrate exhibit excellent resistant performance of rolling contact fatigue （RCF）.
文摘The effect of fin attachment on the thermal stress reduction of exhaust manifold of an off road diesel engine(Komatsu HD325-6) was investigated.For doing this,coupled thermo-fluid-solid analysis of exhaust manifold of the off road diesel engine was carried out.The thermal analysis,including thermal flow,thermal stress,and the thermal deformation of the manifold was investigated.The flow inside the manifold was simulated and then its properties including velocity,pressure,and temperature were obtained.The flow properties were transferred to the solid model and then the thermal stresses and the thermal deformations of the manifold under different operating conditions were calculated.Finally,based on the predicted thermal stresses and thermal deformations of the manifold body shell,two fin types as well as body shell thickness increase were applied in the critical induced thermal stress area of the manifold to reduce the thermal stress and thermal deformation.The results of the above modifications show that the combined modifications,i.e.the thickness increase and the fin attachment,decrease the thermal stresses by up to 28% and the contribution of the fin attachment in this reduction is much higher compared to the shell thickness increase.
基金Project(2012CB619503)supported by the National Basic Research Program of ChinaProject(2013AH100055)supported by the Foshan Civic Technological Innovation Foundation,China
文摘The through-thickness corrosion inhomogeneity of 7050-T7451 Al alloy thick plate was studied using immersion tests, potentiodynamic polarization, electrochemical impedance spectroscopy(EIS), slow strain rate testing(SSRT) technique combined with optical microscopy(OM), scanning electron microscopy(SEM) and transmission electron microscopy(TEM). The results show that the through-thickness corrosion resistance is ranked in the order of T/2>surface>T/4. And the 75 mm-thick 7050 alloy plate presents better corrosion resistance than the 35 mm-thick plate. The results are discussed in terms of the combined effect of recrystallization and cooling rate in quenching. Alloy with lower volume fraction of recrystallization and smaller grain aspect ratio displays better corrosion resistance. The lower corrosion resistance caused by the slower cooling rate results from the higher coverage rate of grain boundary precipitates and larger width of precipitate free zone.
基金Project(2014CB046600)supported by the National Basic Research Program of China
文摘In order to explore drum instability problems of thinning spinning ultra thin-walled tubes with large diameter-to-hickness ratio, experiments of thinning spinning ultra thin-walled tubes with different clearances between the mandrel and the tube were carried out. The phenomena of drum instability were analyzed. Drum instability mechanism was studied. The important influence of the mandrel-locked ring on stable thinning spinning was found. Besides, two important parameters, namely drum ratio and drum stiffness, were proposed to characterize the drum instability of spinning ultra thin-walled tubes with large diameter-to-thickness ratio. What's more, numerical simulations were applied to explore the influences of different clearance ratios and diameter-to-thickness ratios on the drum instability. As a result, it is found that the mandrel-locked ring is the key to the stability and precision of spinning; drum ratio can reflect the degree of the deformation of the tubes; drum stiffness is a comprehensive index to measure the influences of the tube's own parameters on the spinning instability; both the clearance ratio and diameter-thickness ratio have significant influences on the drum ratio and drum stiffness.
基金Projects(50525516, 50875062 ) supported by the National Natural Science Foundation of China
文摘A new process of hydroforming with controllable radial pressure was proposed to overcome difficulties in the forming of low plastic materials and large height-to-diameter ratio workpieces. A typical 5A06 aluminum alloy dome was numerically and experimentally investigated. The reasons for typical defects were analyzed under different radial pressures. Effects of radial pressure on the thickness distribution were discussed and optimal radial pressure was determined. It is shown by numerical simulations and experiment that a cup with a drawing ratio of 2.4 is formed by the new process of hydroforming with controllable radial pressure. It is significantly effective for the forming of low plastic materials and large height-to-diameter ratio workpieees. Two typical thinning points exit along the dome wall. With the radial pressure, thinning is decreased effectively at the two points, the dome forming is achieved and thickness distribution is more uniform.
基金Project(51074051) supported by the National Natural Science Foundation of ChinaProject(N110307001) supported by the Fundamental Research Funds for the Central Universities,China
文摘As the spring equation is limited to the accuracy of mill stiffness and the linearity of the mill spring curve, the traditional gaugemeter automatic gauge control(GM-AGC) system based on spring equation cannot meet the requirements of practical production. In allusion to this problem, a kind of novel GM-AGC system based on mill stretch characteristic curve was proposed. The error existing in calculating strip thickness by spring equation were analyzed first. And then the mill stretch characteristic curve which could effectively eliminate the influence of mill stiffness was described. The novel GM-AGC system has been applied successfully in a hot strip mill, the application results show that the thickness control precision is improved significantly, with the novel GM-AGC system, over 98.6% of the strip thickness deviation of 3.0 mm class can be controlled within the target tolerances of ±20 μm.
基金Project(51005258) supported by the National Natural Science Foundation of ChinaProject(CDJZR12130065) supported by the Fundamental Research Funds for the Central Universities,China
文摘Although multi-stage incremental sheet forming has always been adopted instead of single-stage forming to form parts with a steep wall angle or to achieve a high forming performance, it is largely dependent on empirical designs. In order to research multi-stage forming further, the effect of forming stages(n) and angle interval between the two adjacent stages(Δα) on thickness distribution was investigated. Firstly, a finite element method(FEM) model of multi-stage incremental forming was established and experimentally verified. Then, based on the proposed simulation model, different strategies were adopted to form a frustum of cone with wall angle of 30° to research the thickness distribution of multi-pass forming. It is proved that the minimum thickness increases largely and the variance of sheet thickness decreases significantly as the value of n grows. Further, with the increase of Δα, the minimum thickness increases initially and then decreases, and the optimal thickness distribution is achieved with Δα of 10°.Additionally, a formula is deduced to estimate the sheet thickness after multi-stage forming and proved to be effective. And the simulation results fit well with the experimental results.
基金Project(51805416) supported by the National Natural Science Foundation of ChinaProject(2019QNRC001) supported by the Young Elite Scientists Sponsorship Program by CAST,China+1 种基金Project(2021JJ20059) supported by the Hunan Provincial Natural Science Foundation for Excellent Young Scholars,ChinaProject(2019RS1002) supported by the Huxiang High-Level Talent Gathering Project of Hunan Province,China。
文摘As one of the advanced and efficient means of joining,the clinching process is capable of joining sheets with different materials or different sheet thicknesses.In this article,a novel modified clinching process,i.e.,the dieless clinching process,was executed to join AA6061 aluminum alloy with sheet thicknesses of 1.5,2.0,2.5 and 3.0 mm according to different sheet stack-ups.The geometrical characteristics,microhardness distribution,failure behavior,static strength,absorbed energy and instantaneous stiffness of the novel dieless joint were gotten and investigated.The results indicated that the sheet thickness ratio has a notable effect on the failure behavior and mechanical properties of the novel dieless clinched joint,and a relatively large sheet thickness ratio can improve the joint performance when joining sheets with different sheet thicknesses.
基金Project(51164030)supported by the National Natural Science Foundation of China
文摘For contact dominated numerical control(NC) bending process of tube, the effect of friction on bending deformation behaviors should be focused on to achieve precision bending forming. A three dimensional(3D) elastic-plastic finite element(FE) model of NC bending process was established under ABAQUS/Explicit platform, and its reliability was validated by the experiment. Then, numerical study on bending deformation behaviors under different frictions between tube and various dies was explored from multiple aspects such as wrinkling, wall thickness change and cross section deformation. The results show that the large friction of wiper die-tube reduces the wrinkling wave ratio η and cross section deformation degree ΔD and increases the wall thinning degree Δt. The large friction of mandrel-tube causes large η, Δt and ΔD, and the onset of wrinkling near clamp die. The large friction of pressure die-tube reduces Δt and ΔD, and the friction on this interface has little effect on η. The large friction of bending die-tube reduces η and ΔD, and the friction on this interface has little effect on Δt. The reasonable friction coefficients on wiper die-tube, mandrel-tube, pressure die-tube and bending die-tube of 21-6-9(0Cr21Ni6Mn9N) stainless steel tube in NC bending are 0.05-0.15, 0.05-0.15, 0.25-0.35 and 0.25-0.35, respectively. The results can provide a guideline for applying the friction conditions to establish the robust bending environment for stable and precise bending deformation of tube bending.
基金Project(2012CB619503)supported by State Key Fundamental Research Program of China
文摘The strength, fracture toughness and fatigue crack growth resistance of 7050-T7451 aluminum alloy plate with different thicknesses （35 mm and 160 ram） were investigated by means of optical microscope, scanning electron microscope and transmission electron microscope. The results show that thicker plate has lower strength and fracture toughness but higher fatigue crack growth resistance, by comparison to the thinner plate. The drop of strength is mainly attributed to grain coarsening in the thicker plate, and the increased degree of recrystallization results in the loss of Kio However, the coarsened grains in the thicker plate make cracks deflected and closure effect enhanced due to surface roughness increased. For both of plates, in the fracture surface subjected plain strain, a transition from transgranular dimpled fracture to intergranular dimpled fracture is observed during the fracture process.
基金Project(2021YFB3400903) supported by the National Key R&D Program of ChinaProject(1053320211480) supported by the Science and Technology Innovation Project of Graduate Students of Central South University,China。
文摘Creep ageing forming(CAF)has been widely used in the aerospace engineering,but how to optimize the processing conditions,especially under complex stress state of the CAF process for large-size components produced by friction-stir welding is still a great challenge to now.In this work,the creep ageing behaviors and underlying microstructure evolution of a thick friction-stir welded Al-Cu alloy plate after CAF process under different stress levels are systematically investigated.The creep strain and the strength of the joint are both significantly increased when the stress is close to the average yield strength of the initial weld joint.The grain size reduces while the local strain and dislocation density increase from top to bottom of the NZ;hence,the bottom layer of the weld joint exhibits higher creep strain and steady-stage creep strain rate during the CAF process.The results reveal that the gradient microstructures sensitive to the stress level effectively govern the creep-ageing performance from the upper to the bottom layer in a thick friction stir welded Al-Cu alloy plate.Rationally increasing the initial dislocation density of the weld joint can both enhance the tensile properties and promote the creep deformation of the weld joint for CAF process.
基金Projects(50778181, 51178472) supported by the National Natural Science Foundation of China Project(2007045) supported by the Transportation Department of Hunan Province,China
文摘Finite element method was performed to investigate the influences of beam stiffness, foundation width and cushion thickness on the beating capacity of beam foundation on underlying weak laminated clay. The comparison between numerical results and results from field test including plate-bearing test and foundation settlement observation shows reasonable agreement. According to the numerical results, the beam width, length, cross section and cushion thickness were optimized. The results show that the stresses in subgrade soil decrease greatly with increasing the cushion thickness and width of foundation. However, the foundation settlement and influencing depth of displacement also increase correspondingly under conditions of relatively thinner cushion thickness. For the foundations on underlying weak layer, increasing foundation width merely might be inadequate for improving the bearing capacity, and the appropriate width and cushion thickness depend on the response of subgrade. A comparison between rigid and flexible beams was also discussed. The influence of a flexible beam foundation on subgrade is relatively smaller under the same loading conditions, and the flexible beam foundation appears more adaptable to various subgrades. The proposed flexible beam foundation was adopted in engineering. According to the calculation results, beam width of 2.4 m and cushion thickness of 0.8 m are proposed, and a flexible beam foundation is applied in the optimized design, which is confirmed reasonable by the actual engineering.
