近日,中国农业科学院农业质量标准与检测技术研究所畜产品质量安全创新团队开发了一种小巧便携、便于现场应用的牛奶体细胞计数仪,实现了对生鲜乳中体细胞的快速、准确计数。相关研究成果发表在《食品科学与技术趋势(Trends in Food Sci...近日,中国农业科学院农业质量标准与检测技术研究所畜产品质量安全创新团队开发了一种小巧便携、便于现场应用的牛奶体细胞计数仪,实现了对生鲜乳中体细胞的快速、准确计数。相关研究成果发表在《食品科学与技术趋势(Trends in Food Science&Technology)》《分析化学杂志(Analytica Chimica Acta)》上。生鲜乳体细胞数是奶牛乳房炎早期诊断和乳品质量评估的关键指标。然而,现有的奶牛加州乳房炎诊断测试法不能给出准确体细胞数,无法实现隐性乳房炎早期诊断,乳品企业生鲜乳质量检验依赖进口流式细胞仪设备及试剂耗材,成本高且存在断供风险。展开更多
To evaluate the role of glucose transporter- l (GLUT1) in the glucose uptake of glomerular mesangial cells. Methods. Cultured C57/SJL mouse mesangial cells were used in the study. The expression of GLUT1 mRNA was dete...To evaluate the role of glucose transporter- l (GLUT1) in the glucose uptake of glomerular mesangial cells. Methods. Cultured C57/SJL mouse mesangial cells were used in the study. The expression of GLUT1 mRNA was detected by RT- PCR. The expression of GLUT1 protein was detected by immunofluorescence and flow cytometry. The uptake of glucose and its kinetics were determined by 2- deoxy- [3H]- D- glucose uptake. Results. Both GLUT1 mRNA and protein were found in mouse glomerular mesangial cells. 2- deoxy- D- glucose uptake and kinetics assay showed that this glucose transporter had high affinity for glucose and the glucose uptake specificity was further confirmed by phloretin. Conclusion. Functional GLUT1 did present in mouse mesangial cells cultured in vitro and it might be the predominant transporter mediated the uptake of glucose into mesangial cells.展开更多
基金This work was supported by the National Natural Science Foundation of China (No.39870288)
文摘To evaluate the role of glucose transporter- l (GLUT1) in the glucose uptake of glomerular mesangial cells. Methods. Cultured C57/SJL mouse mesangial cells were used in the study. The expression of GLUT1 mRNA was detected by RT- PCR. The expression of GLUT1 protein was detected by immunofluorescence and flow cytometry. The uptake of glucose and its kinetics were determined by 2- deoxy- [3H]- D- glucose uptake. Results. Both GLUT1 mRNA and protein were found in mouse glomerular mesangial cells. 2- deoxy- D- glucose uptake and kinetics assay showed that this glucose transporter had high affinity for glucose and the glucose uptake specificity was further confirmed by phloretin. Conclusion. Functional GLUT1 did present in mouse mesangial cells cultured in vitro and it might be the predominant transporter mediated the uptake of glucose into mesangial cells.
