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EIF2B5表达下调的人星形胶质细胞与人神经元在内质网应激后细胞存活及miRNA表达的差异
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作者 陈娜 王静敏 +1 位作者 姜玉武 吴晔 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2022年第11期2230-2239,共10页
目的探讨白质消融性白质脑病中胶质细胞选择性受累而神经元受累轻微的原因。方法将EIF2B5-RNAi表达载体转染至人星形胶质细胞和人神经元,检测基础状态下及内质网应激(endoplasmic reticulum stress,ERS)后细胞凋亡和活力,检测参与ERS调... 目的探讨白质消融性白质脑病中胶质细胞选择性受累而神经元受累轻微的原因。方法将EIF2B5-RNAi表达载体转染至人星形胶质细胞和人神经元,检测基础状态下及内质网应激(endoplasmic reticulum stress,ERS)后细胞凋亡和活力,检测参与ERS调控的已知和未知miRNA,筛选EIF2B5-RNAi人星形胶质细胞在ERS后miRNA变化。结果与EIF2B5-RNAi人神经元相比,星形胶质细胞自发凋亡及细胞活力下降。较之神经元,更多miRNA参与星形胶质细胞ERS刺激后的调控,EIF2B5-RNAi组参与调控的miRNA数目显著减少。聚类分析发现,5条已知miRNA是通路连接的关键组分。结论人星形胶质细胞在ERS后可能更加依赖众多促细胞增殖分化的miRNA修复,而EIF2B5-RNAi人星形胶质细胞存在自发凋亡,ERS后严重减少的miRNA可能导致细胞无法存活。 展开更多
关键词 白质消融性白质脑病 人星形胶质细胞 人神经元 内质网应激 凋亡 miRNA表达差异
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Analysis and optimization of variable depth increments in sheet metal incremental forming 被引量:1
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作者 李军超 王宾 周同贵 《Journal of Central South University》 SCIE EI CAS 2014年第7期2553-2559,共7页
A method utilizing variable depth increments during incremental forming was proposed and then optimized based on numerical simulation and intelligent algorithm.Initially,a finite element method(FEM) model was set up a... A method utilizing variable depth increments during incremental forming was proposed and then optimized based on numerical simulation and intelligent algorithm.Initially,a finite element method(FEM) model was set up and then experimentally verified.And the relation between depth increment and the minimum thickness tmin as well as its location was analyzed through the FEM model.Afterwards,the variation of depth increments was defined.The designed part was divided into three areas according to the main deformation mechanism,with Di(i=1,2) representing the two dividing locations.And three different values of depth increment,Δzi(i=1,2,3) were utilized for the three areas,respectively.Additionally,an orthogonal test was established to research the relation between the five process parameters(D and Δz) and tmin as well as its location.The result shows that Δz2 has the most significant influence on the thickness distribution for the corresponding area is the largest one.Finally,a single evaluating indicator,taking into account of both tmin and its location,was formatted with a linear weighted model.And the process parameters were optimized through a genetic algorithm integrated with an artificial neural network based on the evaluating index.The result shows that the proposed algorithm is satisfactory for the optimization of variable depth increment. 展开更多
关键词 incremental forming numerical simulation variable depth increment genetic algorithm OPTIMIZATION
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