利用Caco-2细胞模型研究不同浓度硫酸铜,微米氧化铜,纳米氧化铜(铜水平2、4、8、16 mg/L)对细胞铜-ATP酶、二价金属离子转运体1(Divalent Metal Transporter 1,DMT1)、金属硫蛋白(metallothionein,MT)含量的影响。结果显示,培养液中添...利用Caco-2细胞模型研究不同浓度硫酸铜,微米氧化铜,纳米氧化铜(铜水平2、4、8、16 mg/L)对细胞铜-ATP酶、二价金属离子转运体1(Divalent Metal Transporter 1,DMT1)、金属硫蛋白(metallothionein,MT)含量的影响。结果显示,培养液中添加铜离子能提高Caco-2细胞铜-ATP酶、MT含量,低浓度铜离子能提高细胞DMT1含量,高浓度铜离子降低细胞DMT1含量。纳米氧化铜对几种铜转运蛋白的作用与硫酸铜类似,但纳米氧化铜对铜-ATP酶、MT、DMT1含量的影响更加明显。展开更多
二价金属离子转运蛋白1(divalent metal transporter 1,DMT1)是一种在哺乳动物广泛表达的金属离子转运载体,参与机体内多种金属离子的转运。本文综述DMT1分子结构与分布、生理功能及其对二价金属离子吸收的调控机制,旨在通过对DMT1在微...二价金属离子转运蛋白1(divalent metal transporter 1,DMT1)是一种在哺乳动物广泛表达的金属离子转运载体,参与机体内多种金属离子的转运。本文综述DMT1分子结构与分布、生理功能及其对二价金属离子吸收的调控机制,旨在通过对DMT1在微量元素吸收中的作用机制的研究,来提高动物微量元素的吸收效率和利用率。展开更多
The effects of Mg 2+, Ca 2+, Mn 2+, Co 2+ and Ni 2+ on the thermal stability of Salmon Sperm DNA were investigated by employing differential scanning calorimetry method. For the divalent cations, the shielding effect ...The effects of Mg 2+, Ca 2+, Mn 2+, Co 2+ and Ni 2+ on the thermal stability of Salmon Sperm DNA were investigated by employing differential scanning calorimetry method. For the divalent cations, the shielding effect plays the dominant role in the interaction of nucleic acid chains when they are in low concentrations, as reflected in the moderate increasing of DNA melting temperature. Along with increasing concentration, both alkaline earth metal ions and transitional metal ions were found to lower the DNA melting temperature, indicating possible direct interaction between the divalent cations with the bases in the DNA molecules. The effectiveness increases with the order Mg 2+, Ca 2+, Mn 2+, Co 2+ and Ni 2+. It was found for the first time that the presence of Mn 2+ triggered the biphasic-melting behavior of the Salmon Sperm DNA when the concentration of the cation was increased to a value about 2 times of phosphorous in DNA.展开更多
文摘利用Caco-2细胞模型研究不同浓度硫酸铜,微米氧化铜,纳米氧化铜(铜水平2、4、8、16 mg/L)对细胞铜-ATP酶、二价金属离子转运体1(Divalent Metal Transporter 1,DMT1)、金属硫蛋白(metallothionein,MT)含量的影响。结果显示,培养液中添加铜离子能提高Caco-2细胞铜-ATP酶、MT含量,低浓度铜离子能提高细胞DMT1含量,高浓度铜离子降低细胞DMT1含量。纳米氧化铜对几种铜转运蛋白的作用与硫酸铜类似,但纳米氧化铜对铜-ATP酶、MT、DMT1含量的影响更加明显。
文摘二价金属离子转运蛋白1(divalent metal transporter 1,DMT1)是一种在哺乳动物广泛表达的金属离子转运载体,参与机体内多种金属离子的转运。本文综述DMT1分子结构与分布、生理功能及其对二价金属离子吸收的调控机制,旨在通过对DMT1在微量元素吸收中的作用机制的研究,来提高动物微量元素的吸收效率和利用率。
文摘The effects of Mg 2+, Ca 2+, Mn 2+, Co 2+ and Ni 2+ on the thermal stability of Salmon Sperm DNA were investigated by employing differential scanning calorimetry method. For the divalent cations, the shielding effect plays the dominant role in the interaction of nucleic acid chains when they are in low concentrations, as reflected in the moderate increasing of DNA melting temperature. Along with increasing concentration, both alkaline earth metal ions and transitional metal ions were found to lower the DNA melting temperature, indicating possible direct interaction between the divalent cations with the bases in the DNA molecules. The effectiveness increases with the order Mg 2+, Ca 2+, Mn 2+, Co 2+ and Ni 2+. It was found for the first time that the presence of Mn 2+ triggered the biphasic-melting behavior of the Salmon Sperm DNA when the concentration of the cation was increased to a value about 2 times of phosphorous in DNA.
