The enhancement of chalcopyrite bioleaching with an enriched microbial community by acidified seawater was studied,and the enhancing mechanism was analyzed.The microbial community was enriched at the Dabaoshan mine si...The enhancement of chalcopyrite bioleaching with an enriched microbial community by acidified seawater was studied,and the enhancing mechanism was analyzed.The microbial community was enriched at the Dabaoshan mine site,and the treated ore sample had high concentrations of chalcopyrite and galena.The experimental results show that copper extraction from chalcopyrite with an enriched microbial community in seawater was promoted from 13.1%to 62.1%by acidification in comparison with that without acidification.Further analyses of the solutions,solid residues and microbial compositions by scanning electron microscopy,X-ray diffraction,Raman spectroscopy,Fourier transform infrared spectroscopy and 16 S rDNA sequencing revealed the promoting effects of acidified seawater.This acidification can increase the biodissolution of chalcopyrite to increase the concentration of iron ions and maintain the redox potential in the range of 360−410 mV.The latter produces an optimal redox environment conducive to chalcopyrite dissolution via Cu_(2)S.The adaptability of the microbial community to a high-salt environment is improved.Chloride ions at 580 mmol/L improve the leaching kinetics of chalcopyrite by increasing the porosity and noncrystallinity of the intermediate elemental sulfur.This study provides a promising way to bioleaching copper minerals using seawater for areas with freshwater shortages.展开更多
Motivated by the business model called“community group buying”(CGB),which has emerged in China and some countries in Southeast Asia,such as Singapore and Indonesia,we develop algorithms that could help CGB platforms...Motivated by the business model called“community group buying”(CGB),which has emerged in China and some countries in Southeast Asia,such as Singapore and Indonesia,we develop algorithms that could help CGB platforms match consumers with stage-stations(the picking up center under the CGB mode).By altering the fundamental design of the existing hierarchy algorithms,improvements are achieved.It is proven that our method has a faster running speed and greater space efficiency.Our algorithms avoid traversal and compress the time complexities of matching a consumer with a stage-station and updating the storage information to O(logM)and O(MlogG),where M is the number of stage-stations and G is that of the platform’s stock-keeping units.Simulation comparisons of our algorithms with the current methods of CGB platforms show that our approaches can effectively reduce delivery costs.An interesting observation of the simula-tions is worthy of note:Increasing G may incur higher costs since it makes inventories more dispersed and delivery prob-lems more complicated.展开更多
采用累积频率的统计方法和Community Climate Model 3(CCM3)模拟的10年逐日降水结果,分析了模拟的夏季极端降水事件的时空分布特征.结果表明,CCM3模拟的极端降水阈值的大值区主要在我国黄河和长江流域的上游、印度半岛及其邻近海域和孟...采用累积频率的统计方法和Community Climate Model 3(CCM3)模拟的10年逐日降水结果,分析了模拟的夏季极端降水事件的时空分布特征.结果表明,CCM3模拟的极端降水阈值的大值区主要在我国黄河和长江流域的上游、印度半岛及其邻近海域和孟加拉湾及其北部地区.CCM3能够模拟出我国长江流域极端降水量与极端降水日数显著增加的趋势.对极端降水平均强度、降水日数以及极端降水量与总降水量比值的经验正交函数(EOF)分析可知,我国大部分地区的极端降水基本呈现同相变化,且以长江和黄河中游地区较为显著.CCM3模式基本能够模拟出观测到的极端降水阈值与总降水、极端降水日数及其距平的高空间相关性.展开更多
【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact ...【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact of this soaking method on the fungal and bacterial community structures using next-generation sequencing technology(NGS).【Methods】Lily bulbs were soaked in a seed treating agent containing beneficial microbes(SP treatment)for 4 hours.Subsequently,they were planted in soil in July and sampled in September to assess plant growth,rhizosphere soil physicochemical properties,and microorganism community structures.In addition,we employed the software PICRUSt and FUNGuild to predict bacterial pathways and fungal functions.【Results】Under SP treatment,there were significant alterations in fungi and bacteria community structures,accompanied by improved soil nutrient status.Notably,the relative abundance of dominant microorganism groups,such as the fungi Basidiomycota,Pseudeurotium,Cladophialophora,Microascus,and Dactylonectria,as well as the bacteria Proteobacteria,Chloroflexi,Ochrobactrium,Lysobacter,and RB41,underwent notable changes.Microorganism function prediction results indicated a reduction in pathotrophic fungi(including plant pathogens)and an increase in endophytic and saprotrophic fungi under SP treatment.Among the top 20 metabolism pathways,80%were upregulated in SP treatment compared to the CK.【Conclusions】Seed soaking with beneficial microbial strain promotes the growth of Lanzhou lily bulbs.The beneficial microorganisms play a crucial role in regulating soil microbial structures,enhancing the accumulation of endophytic fungi,reducing the abundance of pathogens,and improving soil functions.Furthermore,specific microbial groups are found to be involved in maintaining soil health.展开更多
基金Project(2022YFC2105300)supported by the National Key Research and Development Program of ChinaProjects(41802038,51774342)supported by the National Natural Science Foundation of China。
文摘The enhancement of chalcopyrite bioleaching with an enriched microbial community by acidified seawater was studied,and the enhancing mechanism was analyzed.The microbial community was enriched at the Dabaoshan mine site,and the treated ore sample had high concentrations of chalcopyrite and galena.The experimental results show that copper extraction from chalcopyrite with an enriched microbial community in seawater was promoted from 13.1%to 62.1%by acidification in comparison with that without acidification.Further analyses of the solutions,solid residues and microbial compositions by scanning electron microscopy,X-ray diffraction,Raman spectroscopy,Fourier transform infrared spectroscopy and 16 S rDNA sequencing revealed the promoting effects of acidified seawater.This acidification can increase the biodissolution of chalcopyrite to increase the concentration of iron ions and maintain the redox potential in the range of 360−410 mV.The latter produces an optimal redox environment conducive to chalcopyrite dissolution via Cu_(2)S.The adaptability of the microbial community to a high-salt environment is improved.Chloride ions at 580 mmol/L improve the leaching kinetics of chalcopyrite by increasing the porosity and noncrystallinity of the intermediate elemental sulfur.This study provides a promising way to bioleaching copper minerals using seawater for areas with freshwater shortages.
基金supported by the National Natural Science Foundation of China(71991464,71921001)Fundamental Research Funds for the Central Universities,General Program(WK2040000053)Key Program(YD2040002027)。
文摘Motivated by the business model called“community group buying”(CGB),which has emerged in China and some countries in Southeast Asia,such as Singapore and Indonesia,we develop algorithms that could help CGB platforms match consumers with stage-stations(the picking up center under the CGB mode).By altering the fundamental design of the existing hierarchy algorithms,improvements are achieved.It is proven that our method has a faster running speed and greater space efficiency.Our algorithms avoid traversal and compress the time complexities of matching a consumer with a stage-station and updating the storage information to O(logM)and O(MlogG),where M is the number of stage-stations and G is that of the platform’s stock-keeping units.Simulation comparisons of our algorithms with the current methods of CGB platforms show that our approaches can effectively reduce delivery costs.An interesting observation of the simula-tions is worthy of note:Increasing G may incur higher costs since it makes inventories more dispersed and delivery prob-lems more complicated.
文摘采用累积频率的统计方法和Community Climate Model 3(CCM3)模拟的10年逐日降水结果,分析了模拟的夏季极端降水事件的时空分布特征.结果表明,CCM3模拟的极端降水阈值的大值区主要在我国黄河和长江流域的上游、印度半岛及其邻近海域和孟加拉湾及其北部地区.CCM3能够模拟出我国长江流域极端降水量与极端降水日数显著增加的趋势.对极端降水平均强度、降水日数以及极端降水量与总降水量比值的经验正交函数(EOF)分析可知,我国大部分地区的极端降水基本呈现同相变化,且以长江和黄河中游地区较为显著.CCM3模式基本能够模拟出观测到的极端降水阈值与总降水、极端降水日数及其距平的高空间相关性.
文摘【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact of this soaking method on the fungal and bacterial community structures using next-generation sequencing technology(NGS).【Methods】Lily bulbs were soaked in a seed treating agent containing beneficial microbes(SP treatment)for 4 hours.Subsequently,they were planted in soil in July and sampled in September to assess plant growth,rhizosphere soil physicochemical properties,and microorganism community structures.In addition,we employed the software PICRUSt and FUNGuild to predict bacterial pathways and fungal functions.【Results】Under SP treatment,there were significant alterations in fungi and bacteria community structures,accompanied by improved soil nutrient status.Notably,the relative abundance of dominant microorganism groups,such as the fungi Basidiomycota,Pseudeurotium,Cladophialophora,Microascus,and Dactylonectria,as well as the bacteria Proteobacteria,Chloroflexi,Ochrobactrium,Lysobacter,and RB41,underwent notable changes.Microorganism function prediction results indicated a reduction in pathotrophic fungi(including plant pathogens)and an increase in endophytic and saprotrophic fungi under SP treatment.Among the top 20 metabolism pathways,80%were upregulated in SP treatment compared to the CK.【Conclusions】Seed soaking with beneficial microbial strain promotes the growth of Lanzhou lily bulbs.The beneficial microorganisms play a crucial role in regulating soil microbial structures,enhancing the accumulation of endophytic fungi,reducing the abundance of pathogens,and improving soil functions.Furthermore,specific microbial groups are found to be involved in maintaining soil health.
