The study was designated to explore the physiological mechanism of cold tolerance enhanced by phosphate in rice. An experiment was conducted to investigate the effects of different levels of phosphate fertilizer on co...The study was designated to explore the physiological mechanism of cold tolerance enhanced by phosphate in rice. An experiment was conducted to investigate the effects of different levels of phosphate fertilizer on cold tolerance and its related physiological parameters in rice seedings （chilling-sensitive cv. Changbai 9 and chilling-tolerant cv. Jijing 81） under low temperature stress. At the same time, the identification of cold tolerance was conducted. Compared with the normal temperature treatment, the relative chlorophyll content, photosynthesis rate, Fv/Fm and qP decreased and index of unsaturated fatty acid increased in rice under low temperature stress. The effect of chilling-sensitive cultivars was more than that of chilling-tolerant cultivars, more phosphorus fertilizer properly improved seedling quality of rice, slowed relative chlorophyll content dropping degree of rice seeding, increased photosynthesis rate, Fv/Fm, qP and index of unsaturated fatty acids, and enhanced the ability to chilling-tolerant cultivars under low temperature. The effect on chilling-tolerant cultivars was significantly higher than that on chilling sensitive cultivars by applying more phosphorus fertilizer. Phosphate regulated photosynthetic physiology and membrane fluidity to reduce injury by low temperature, and increasd the cold tolerance capacity of rice.展开更多
本试验以黄瓜‘新泰密刺’和番茄‘D2856’为试材,研究在低温弱光(15℃/8℃,80μmol·m^(-2)·s^(-1),low temperature and low light,LL)下,丛枝菌根真菌(Arbuscular mycorrhizal fungi,AMF)和宛氏拟青霉(Paecilomyces variot...本试验以黄瓜‘新泰密刺’和番茄‘D2856’为试材,研究在低温弱光(15℃/8℃,80μmol·m^(-2)·s^(-1),low temperature and low light,LL)下,丛枝菌根真菌(Arbuscular mycorrhizal fungi,AMF)和宛氏拟青霉(Paecilomyces variotii)提取物智能聪(ZNC)对黄瓜和番茄幼苗生长及其抗氧化系统活性的影响。幼苗两叶一心时LL处理7 d,LL下根施基质重量比1%AMF(LL+AMF)和喷施10 mL 10 ng·mL^(-1) ZNC(LL+ZNC),以正常温光为对照(28℃/18℃,600μmol·m^(-2)·s^(-1),CK)。结果表明,与CK相比,LL显著降低黄瓜和番茄壮苗指数和根系活力,抑制植株生长,且黄瓜和番茄幼苗SOD、POD活性及可溶性糖和蛋白含量增加,说明LL引起植株膜脂过氧化。LL下施用AMF和ZNC显著提高黄瓜和番茄的可溶性糖含量以及POD和CAT活性。其中,LL下施用AMF和ZNC黄瓜的壮苗指数显著提高,较LL分别提高19.4%和11.1%;LL+AMF和LL+ZNC番茄幼苗根系活力及可溶性糖含量较LL分别提高22.7%、14.8%和31.9%、26.9%。综上所述,本试验条件下AMF和ZNC可通过提高黄瓜和番茄幼苗抗氧化酶活性以及渗透调节能力,减轻低温弱光造成的植株膜脂过氧化水平,进而缓解对黄瓜和番茄幼苗的生长抑制。展开更多
基金Supported by the Special Agricultural Project of Agricultural Department (200903003)the Agricultural Modernization Project in the Science and Technology Development Plan of Jilin Province (2009-2010) the High-yield Project of Science and Technology Department (2011BAD16B10)
文摘The study was designated to explore the physiological mechanism of cold tolerance enhanced by phosphate in rice. An experiment was conducted to investigate the effects of different levels of phosphate fertilizer on cold tolerance and its related physiological parameters in rice seedings （chilling-sensitive cv. Changbai 9 and chilling-tolerant cv. Jijing 81） under low temperature stress. At the same time, the identification of cold tolerance was conducted. Compared with the normal temperature treatment, the relative chlorophyll content, photosynthesis rate, Fv/Fm and qP decreased and index of unsaturated fatty acid increased in rice under low temperature stress. The effect of chilling-sensitive cultivars was more than that of chilling-tolerant cultivars, more phosphorus fertilizer properly improved seedling quality of rice, slowed relative chlorophyll content dropping degree of rice seeding, increased photosynthesis rate, Fv/Fm, qP and index of unsaturated fatty acids, and enhanced the ability to chilling-tolerant cultivars under low temperature. The effect on chilling-tolerant cultivars was significantly higher than that on chilling sensitive cultivars by applying more phosphorus fertilizer. Phosphate regulated photosynthetic physiology and membrane fluidity to reduce injury by low temperature, and increasd the cold tolerance capacity of rice.
文摘本试验以黄瓜‘新泰密刺’和番茄‘D2856’为试材,研究在低温弱光(15℃/8℃,80μmol·m^(-2)·s^(-1),low temperature and low light,LL)下,丛枝菌根真菌(Arbuscular mycorrhizal fungi,AMF)和宛氏拟青霉(Paecilomyces variotii)提取物智能聪(ZNC)对黄瓜和番茄幼苗生长及其抗氧化系统活性的影响。幼苗两叶一心时LL处理7 d,LL下根施基质重量比1%AMF(LL+AMF)和喷施10 mL 10 ng·mL^(-1) ZNC(LL+ZNC),以正常温光为对照(28℃/18℃,600μmol·m^(-2)·s^(-1),CK)。结果表明,与CK相比,LL显著降低黄瓜和番茄壮苗指数和根系活力,抑制植株生长,且黄瓜和番茄幼苗SOD、POD活性及可溶性糖和蛋白含量增加,说明LL引起植株膜脂过氧化。LL下施用AMF和ZNC显著提高黄瓜和番茄的可溶性糖含量以及POD和CAT活性。其中,LL下施用AMF和ZNC黄瓜的壮苗指数显著提高,较LL分别提高19.4%和11.1%;LL+AMF和LL+ZNC番茄幼苗根系活力及可溶性糖含量较LL分别提高22.7%、14.8%和31.9%、26.9%。综上所述,本试验条件下AMF和ZNC可通过提高黄瓜和番茄幼苗抗氧化酶活性以及渗透调节能力,减轻低温弱光造成的植株膜脂过氧化水平,进而缓解对黄瓜和番茄幼苗的生长抑制。
