A novel 6-PSS flexible parallel mechanism was presented,which employed wide-range flexure hinges as passive joints.The proposed mechanism features micron level positioning accuracy over cubic centimeter scale workspac...A novel 6-PSS flexible parallel mechanism was presented,which employed wide-range flexure hinges as passive joints.The proposed mechanism features micron level positioning accuracy over cubic centimeter scale workspace.A three-layer back-propagation(BP) neural network was utilized to the kinematics analysis,in which learning samples containing 1 280 groups of data based on stiffness-matrix method were used to train the BP model.The kinematics performance was accurately calculated by using the constructed BP model with 19 hidden nodes.Compared with the stiffness model,the simulation and numerical results validate that BP model can achieve millisecond level computation time and micron level calculation accuracy.The concept and approach outlined can be extended to a variety of applications.展开更多
Objective In kinesin-3,the neck coil correlates with the following segments to form an extended neck that contains a characteristic hinge diverse from a proline in KIF13B to a long flexible linker in KIF1A.The functio...Objective In kinesin-3,the neck coil correlates with the following segments to form an extended neck that contains a characteristic hinge diverse from a proline in KIF13B to a long flexible linker in KIF1A.The function of this neck hinge for controlling processive movement,however,remains unclear.Methods We made a series of modifications to the neck hinges of KIF13B and KIF1A and tested their movement using a single-molecule motility assay.Results In KIF13B,the insertion of flexible residues before or after the proline differentially impacts the processivity or velocity,while the removal of this proline increases the both.In KIF1A,the deletion of entire flexible neck hinge merely enhances the processivity.The engineering of these hinge-truncated necks of kinesin-3 into kinesin-1 similarly boosts the processive movement of kinesin-1.Conclusion The neck hinge in kinesin-3 controls its processive movement and proper modifications tune the motor motility,which provides a novel strategy to reshape the processive movement of kinesin motors.展开更多
基金Project(2002AA422260) supported by the National High Technology Research and Development Program of ChinaProject(2011-6) supported by CAST-HIT Joint Program,ChinaProject supported by Harbin Institute of Technology (HIT) Overseas Talents Introduction Program,China
文摘A novel 6-PSS flexible parallel mechanism was presented,which employed wide-range flexure hinges as passive joints.The proposed mechanism features micron level positioning accuracy over cubic centimeter scale workspace.A three-layer back-propagation(BP) neural network was utilized to the kinematics analysis,in which learning samples containing 1 280 groups of data based on stiffness-matrix method were used to train the BP model.The kinematics performance was accurately calculated by using the constructed BP model with 19 hidden nodes.Compared with the stiffness model,the simulation and numerical results validate that BP model can achieve millisecond level computation time and micron level calculation accuracy.The concept and approach outlined can be extended to a variety of applications.
文摘Objective In kinesin-3,the neck coil correlates with the following segments to form an extended neck that contains a characteristic hinge diverse from a proline in KIF13B to a long flexible linker in KIF1A.The function of this neck hinge for controlling processive movement,however,remains unclear.Methods We made a series of modifications to the neck hinges of KIF13B and KIF1A and tested their movement using a single-molecule motility assay.Results In KIF13B,the insertion of flexible residues before or after the proline differentially impacts the processivity or velocity,while the removal of this proline increases the both.In KIF1A,the deletion of entire flexible neck hinge merely enhances the processivity.The engineering of these hinge-truncated necks of kinesin-3 into kinesin-1 similarly boosts the processive movement of kinesin-1.Conclusion The neck hinge in kinesin-3 controls its processive movement and proper modifications tune the motor motility,which provides a novel strategy to reshape the processive movement of kinesin motors.
