Objective To explore the sequential effects of hypoxic exercising on miR-27/PPARγand lipid metabolism targetgene and protein expression levels in the obesity rats’liver.Methods 13-week-old male diet-induced obesity ...Objective To explore the sequential effects of hypoxic exercising on miR-27/PPARγand lipid metabolism targetgene and protein expression levels in the obesity rats’liver.Methods 13-week-old male diet-induced obesity rats were randomlydivided into three groups(n=10):normal oxygen concentration quiet group(N),hypoxia quiet group(H),hypoxic exercise group(HE).Exercise training on the horizontal animal treadmill for 1 h/d,5 d/week for a total of 4 week,and the intensity of horizontaltreadmill training was 20 m/min(hypoxic concentration was 13.6%).Comparison of the weights of perirenal fat and epididymal fat in rats across different groups and calculation of Lee’s index based on body weight and body length of rats in each group were done.And the serum concentrations of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high-densitylipoprotein cholesterol(HDL-C)levels were detected.RT-PCR and Western Blot were used to detect the levels of miR-27,PPARγ,CYP7A1 and CD36.Results Hypoxic exercise decreased the expression levels of miR-27 in the obese rats’liver,however,theexpression level of PPARγwas gradually increased.The expression levels of miR-27 in HE group were significantly lower than Ngroup(P<0.05).The expression levels of PPARγmRNA in N group were significantly lower than H group(P<0.05),especially lowerthan HE group(P<0.01).The protein expression of PPARγprotein in N group was significantly lower than that other groups(P<0.01).The expression of lipid metabolism-related genes and proteins increased in the obese rats’liver.The expression of CYP7A1mRNA in N group was significantly lower than H group(P<0.05),especially lower than HE group(P<0.01).The expression ofCYP7A1 protein in the obese rats’liver in N group was extremely lower than H group and HE group(P<0.01).The proteinexpression of CD36 in N group was significantly lower than that in HE group(P<0.05).Hypoxia exercise improved the relatedphysiological and biochemical indexes of lipid metabolism disorder.The perirenal fat weight of obese rats in HE group wasextremely lower than N group and H group(P<0.01),and the perirenal fat weight in N group was significantly higher than H group(P<0.05).The epididymal fat weight in N group was significantly higher than H group(P<0.05),and extremely higher than HEgroup(P<0.01).The Lee’s index in HE group was extremely lower than N group and H group(P<0.01).The serum concentration ofTC in obese rats in HE group was extremely lower than N group and H group(P<0.01).The serum concentration of TG in HE groupwas extremely lower than N group and H group(P<0.01).The serum concentration of LDL-C in N group was extremely higher thanHE group(P<0.01).The serum concentration of HDL-C in N group was extremely lower than H group(P<0.01).Conclusion Hypoxiaand hypoxia exercise may negatively regulate the levels of PPARγby inhibiting miR-27 in the obese rats’liver,thereby affecting theexpression of downstream target genes CYP7A1 and CD36,and promoting cholesterol,fatty acid oxidation and HDL-C transport inthe liver,and ultimately the lipid levels in obese rats were improved.The effect of hypoxia exercise on improving blood lipid isbetter than simple hypoxia intervention.展开更多
目的了解中老年2型糖尿病(type 2 diabetes mellitus,T2DM)患者少肌性肥胖(sarcopenic obesity,SO)患病率及不同诊断方法之间的一致性。方法采用系统随机抽样法选取2016年1月至2018年3月于北京地区9家医院内分泌科就诊的≥50岁T2DM患者...目的了解中老年2型糖尿病(type 2 diabetes mellitus,T2DM)患者少肌性肥胖(sarcopenic obesity,SO)患病率及不同诊断方法之间的一致性。方法采用系统随机抽样法选取2016年1月至2018年3月于北京地区9家医院内分泌科就诊的≥50岁T2DM患者,使用生物阻抗法进行身体成分检测;根据2022年欧洲临床营养与代谢学会(European Society for Clinical Nutrition and Metabolism,ESPEN)和欧洲肥胖研究协会(European Association for the Study of Obesity,EASO)方法定义SO,另外3种方法通过肌少症和肥胖的组合进行诊断。肌少症使用2019年亚洲肌少症工作组(Asian Working Group for Sarcopenia,AWGS)建立的标准来定义,肥胖通过体脂(percent of body fat,PBF)、腰围(waist circumference,WC)和体质量指数(body mass index,BMI)来定义。卡方检验进行率的比较,Cohens kappa统计分析比较4种方法的诊断一致性。结果共纳入1125例T2DM受试者,男性586例,年龄[61.2(55.3,67.4)]岁;女性539例,年龄[62.0(56.3,68.1)岁]。使用ESPEN/EASO共识、AWGS+PBF、AWGS+WC和AWGS+BMI标准,中老年T2DM患者SO患病率分别为41.6%、20.4%、30.1%和18.8%。4种方法之间的诊断一致性存在异质性(κ:0.109~0.655)。ESPEN/EASO共识与AWGS+PBF诊断一致性良好(κ:0.655),AWGS+体脂与AWGS+BMI诊断一致性良好(κ:0.637),AWGS+WC与AWGS+BMI(κ:0.359)、与AWGS+PBF诊断一致性中等(κ:0.330)。结论中老年T2DM患者SO患病率高,患病率和诊断一致性在不同诊断方法中存在差异,ESPEN/EASO的共识诊断率最高,AWGS+BMI诊断率最低,ESPEN/EASO共识与AWGS+体脂具有良好的诊断一致性。展开更多
目的:探讨体重校正腰围指数(weight-adjusted waist index,WWI)与美国成人急性、亚急性、慢性疼痛之间的相关性。方法:采用横断面研究,提取1999—2004年美国国家卫生和营养检查调查(National Health and Nutrition Examination Survey,N...目的:探讨体重校正腰围指数(weight-adjusted waist index,WWI)与美国成人急性、亚急性、慢性疼痛之间的相关性。方法:采用横断面研究,提取1999—2004年美国国家卫生和营养检查调查(National Health and Nutrition Examination Survey,NHANES)数据库中关于成人腰围、体质量和疼痛等变量,及性别、年龄、种族、婚姻状况、教育水平、家庭收入、体力活动情况、饮酒、吸烟和糖尿病患病状况等协变量数据。采用多分类Logistic回归分析构建3种模型,评估WWI与急性、亚急性和慢性疼痛之间的相关性。模型1未对协变量进行校正,模型2对年龄、性别、种族、婚姻状况、教育水平和家庭收入情况进行校正,模型3进一步校正了体力活动、饮酒、吸烟和糖尿病患病情况等所有协变量。结果:共纳入12694例参与者,平均年龄为(50.8±18.7)岁,其中9614例(75.74%)未出现超过24 h的疼痛,870例(6.85%)出现急性疼痛,354例(2.79%)出现亚急性疼痛,1856例(14.62%)出现慢性疼痛。所有参与者的WWI为(10.95±0.85)cm/√kg,根据WWI的四分位数分为4组:Q1组为(7.90~10.36)cm/√kg,Q2组为(10.37~10.94)cm/√kg,Q3组为(10.95~11.53)cm/√kg,Q4组为(11.54~15.20)cm/√kg。随着WWI的增加,参与者的急性、慢性疼痛状态的差异有统计学意义(P<0.001)。模型1中,与Q1组相比,Q2组和Q4组的急性疼痛风险降低(Q2组:OR=0.765,95%CI:0.615~0.953,P=0.017;Q4组:OR=0.648,95%CI:0.503~0.835,P<0.001);与Q1组相比,Q2组、Q3组和Q4组的慢性疼痛风险均增加(Q2组:OR=1.365,95%CI:1.149~1.622,P<0.001;Q3组:OR=1.291,95%CI:1.082~1.541,P=0.005;Q4组:OR=1.874,95%CI:1.579~2.224,P<0.001)。模型2中,与Q1组相比,其他3组慢性疼痛风险增加(Q2组:OR=1.359,95%CI:1.137~1.624,P=0.001;Q3组:OR=1.260,95%CI:1.039~1.528,P=0.019;Q4组:OR=1.735,95%CI:1.413~2.132,P<0.001)。模型3中与Q1组相比,Q4组的慢性疼痛风险增加49.2%(OR=1.492,95%CI:1.208~1.842,P<0.001)。在模型2和模型3中,急性疼痛与WWI未见相关性(均P>0.05);3个模型均未发现亚急性疼痛与WWI存在相关性(均P>0.05)。结论:WWI与美国成人急性疼痛、亚急性疼痛之间未见明显相关性,但随着WWI的增加,慢性疼痛风险增加,所以有必要通过大规模前瞻性研究进一步验证这一结论。展开更多
目的研究长期规律体育训练青少年的身体组成情况及相关影响因素。方法本研究为前瞻性队列研究,采用便利抽样法选取2023年6月在济宁市体育训练中心接受长期规律体育训练的青少年,并进行体格测量,获取身高、体重、体质量指数(body mass in...目的研究长期规律体育训练青少年的身体组成情况及相关影响因素。方法本研究为前瞻性队列研究,采用便利抽样法选取2023年6月在济宁市体育训练中心接受长期规律体育训练的青少年,并进行体格测量,获取身高、体重、体质量指数(body mass index,BMI)、血压、心率、腰围、臀围等数据,采用问卷调查获取睡眠时间、电子屏幕使用时间和家庭收入情况。2024年6月重复体格测量及问卷调查,并采用生物电阻抗法补充测量人体成分(体成分体重、骨骼肌量、体脂肪量、体脂率)。以随访期体脂率、BMI和腰围作为因变量,以训练类型(直接对抗性训练或非直接对抗性训练)作为自变量建立线性回归模型,分析训练类型与体脂率、BMI及腰围的相关性。结果共纳入110名青少年(女生39名、男生71名),其中65人接受直接对抗性训练,45人接受非直接对抗性训练,中位年龄为13.21(12.46,14.33)岁,超重或肥胖率为27.27%,腰围偏高率为24.55%,血压偏高率为16.36%。随访期超重或肥胖率为24.55%,腰围偏高率为26.36%,血压偏高率为13.64%,其中血压偏高组的腰围(P=0.034)、腰围偏高率(P=0.011)和体成分体重(P=0.049)均显著高于血压正常组。56.36%的青少年随访期BMI较基线期增加,63.64%的青少年随访期腰围较基线期增加。线性回归模型校正性别、年龄、基线期数据、睡眠时间、电子屏幕使用时间和家庭收入情况后显示,直接对抗性运动与体脂率(β=-6.57,95%CI:-9.37~-3.78,P<0.001)、BMI(β=-1.30,95%CI:-2.50~-0.11,P=0.036)、腰围(β=-2.31,95%CI:-4.40~-0.22,P=0.034)均呈显著负相关性。结论进行长期规律体育训练的青少年中,超重或肥胖、腹型肥胖和血压偏高的情况仍较多,应重视这一群体的肥胖情况以及潜在不良影响。此外,体育运动方式可能会影响青少年人体成分变化,直接对抗性运动有助于降低青少年体脂率、BMI和腰围。展开更多
目的采用meta分析观察运动对改善超重及肥胖人群神经可塑性的价值。方法检索Web of Science、PubMed、PsycINFO及中国知网自建库至2024年8月1日关于运动干预后超重或肥胖人群脑激活变化的功能MRI(fMRI)文献,采用激活似然估计(ALE)法分...目的采用meta分析观察运动对改善超重及肥胖人群神经可塑性的价值。方法检索Web of Science、PubMed、PsycINFO及中国知网自建库至2024年8月1日关于运动干预后超重或肥胖人群脑激活变化的功能MRI(fMRI)文献,采用激活似然估计(ALE)法分析数据,得到脑区激活簇坐标。结果最终共纳入11篇文献,包括186名超重及肥胖受试者。运动后,所有受试者右额中回激活增加,左小脑前叶、左楔前叶及右楔叶激活减少;其中,未成年人右扣带回及右颞上回激活增加,左小脑前叶、双侧中央前回激活减少;成年人双侧豆状核、右额中回、右扣带回及右海马旁回激活增加,双侧楔前叶激活减少。结论运动可改善超重及肥胖人群执行功能、工作记忆、注意力和运动控制相关脑区激活程度,且运动后未成年人与成年人之间改善情况存在差异。展开更多
文摘Objective To explore the sequential effects of hypoxic exercising on miR-27/PPARγand lipid metabolism targetgene and protein expression levels in the obesity rats’liver.Methods 13-week-old male diet-induced obesity rats were randomlydivided into three groups(n=10):normal oxygen concentration quiet group(N),hypoxia quiet group(H),hypoxic exercise group(HE).Exercise training on the horizontal animal treadmill for 1 h/d,5 d/week for a total of 4 week,and the intensity of horizontaltreadmill training was 20 m/min(hypoxic concentration was 13.6%).Comparison of the weights of perirenal fat and epididymal fat in rats across different groups and calculation of Lee’s index based on body weight and body length of rats in each group were done.And the serum concentrations of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high-densitylipoprotein cholesterol(HDL-C)levels were detected.RT-PCR and Western Blot were used to detect the levels of miR-27,PPARγ,CYP7A1 and CD36.Results Hypoxic exercise decreased the expression levels of miR-27 in the obese rats’liver,however,theexpression level of PPARγwas gradually increased.The expression levels of miR-27 in HE group were significantly lower than Ngroup(P<0.05).The expression levels of PPARγmRNA in N group were significantly lower than H group(P<0.05),especially lowerthan HE group(P<0.01).The protein expression of PPARγprotein in N group was significantly lower than that other groups(P<0.01).The expression of lipid metabolism-related genes and proteins increased in the obese rats’liver.The expression of CYP7A1mRNA in N group was significantly lower than H group(P<0.05),especially lower than HE group(P<0.01).The expression ofCYP7A1 protein in the obese rats’liver in N group was extremely lower than H group and HE group(P<0.01).The proteinexpression of CD36 in N group was significantly lower than that in HE group(P<0.05).Hypoxia exercise improved the relatedphysiological and biochemical indexes of lipid metabolism disorder.The perirenal fat weight of obese rats in HE group wasextremely lower than N group and H group(P<0.01),and the perirenal fat weight in N group was significantly higher than H group(P<0.05).The epididymal fat weight in N group was significantly higher than H group(P<0.05),and extremely higher than HEgroup(P<0.01).The Lee’s index in HE group was extremely lower than N group and H group(P<0.01).The serum concentration ofTC in obese rats in HE group was extremely lower than N group and H group(P<0.01).The serum concentration of TG in HE groupwas extremely lower than N group and H group(P<0.01).The serum concentration of LDL-C in N group was extremely higher thanHE group(P<0.01).The serum concentration of HDL-C in N group was extremely lower than H group(P<0.01).Conclusion Hypoxiaand hypoxia exercise may negatively regulate the levels of PPARγby inhibiting miR-27 in the obese rats’liver,thereby affecting theexpression of downstream target genes CYP7A1 and CD36,and promoting cholesterol,fatty acid oxidation and HDL-C transport inthe liver,and ultimately the lipid levels in obese rats were improved.The effect of hypoxia exercise on improving blood lipid isbetter than simple hypoxia intervention.
文摘目的了解中老年2型糖尿病(type 2 diabetes mellitus,T2DM)患者少肌性肥胖(sarcopenic obesity,SO)患病率及不同诊断方法之间的一致性。方法采用系统随机抽样法选取2016年1月至2018年3月于北京地区9家医院内分泌科就诊的≥50岁T2DM患者,使用生物阻抗法进行身体成分检测;根据2022年欧洲临床营养与代谢学会(European Society for Clinical Nutrition and Metabolism,ESPEN)和欧洲肥胖研究协会(European Association for the Study of Obesity,EASO)方法定义SO,另外3种方法通过肌少症和肥胖的组合进行诊断。肌少症使用2019年亚洲肌少症工作组(Asian Working Group for Sarcopenia,AWGS)建立的标准来定义,肥胖通过体脂(percent of body fat,PBF)、腰围(waist circumference,WC)和体质量指数(body mass index,BMI)来定义。卡方检验进行率的比较,Cohens kappa统计分析比较4种方法的诊断一致性。结果共纳入1125例T2DM受试者,男性586例,年龄[61.2(55.3,67.4)]岁;女性539例,年龄[62.0(56.3,68.1)岁]。使用ESPEN/EASO共识、AWGS+PBF、AWGS+WC和AWGS+BMI标准,中老年T2DM患者SO患病率分别为41.6%、20.4%、30.1%和18.8%。4种方法之间的诊断一致性存在异质性(κ:0.109~0.655)。ESPEN/EASO共识与AWGS+PBF诊断一致性良好(κ:0.655),AWGS+体脂与AWGS+BMI诊断一致性良好(κ:0.637),AWGS+WC与AWGS+BMI(κ:0.359)、与AWGS+PBF诊断一致性中等(κ:0.330)。结论中老年T2DM患者SO患病率高,患病率和诊断一致性在不同诊断方法中存在差异,ESPEN/EASO的共识诊断率最高,AWGS+BMI诊断率最低,ESPEN/EASO共识与AWGS+体脂具有良好的诊断一致性。
文摘目的:探讨体重校正腰围指数(weight-adjusted waist index,WWI)与美国成人急性、亚急性、慢性疼痛之间的相关性。方法:采用横断面研究,提取1999—2004年美国国家卫生和营养检查调查(National Health and Nutrition Examination Survey,NHANES)数据库中关于成人腰围、体质量和疼痛等变量,及性别、年龄、种族、婚姻状况、教育水平、家庭收入、体力活动情况、饮酒、吸烟和糖尿病患病状况等协变量数据。采用多分类Logistic回归分析构建3种模型,评估WWI与急性、亚急性和慢性疼痛之间的相关性。模型1未对协变量进行校正,模型2对年龄、性别、种族、婚姻状况、教育水平和家庭收入情况进行校正,模型3进一步校正了体力活动、饮酒、吸烟和糖尿病患病情况等所有协变量。结果:共纳入12694例参与者,平均年龄为(50.8±18.7)岁,其中9614例(75.74%)未出现超过24 h的疼痛,870例(6.85%)出现急性疼痛,354例(2.79%)出现亚急性疼痛,1856例(14.62%)出现慢性疼痛。所有参与者的WWI为(10.95±0.85)cm/√kg,根据WWI的四分位数分为4组:Q1组为(7.90~10.36)cm/√kg,Q2组为(10.37~10.94)cm/√kg,Q3组为(10.95~11.53)cm/√kg,Q4组为(11.54~15.20)cm/√kg。随着WWI的增加,参与者的急性、慢性疼痛状态的差异有统计学意义(P<0.001)。模型1中,与Q1组相比,Q2组和Q4组的急性疼痛风险降低(Q2组:OR=0.765,95%CI:0.615~0.953,P=0.017;Q4组:OR=0.648,95%CI:0.503~0.835,P<0.001);与Q1组相比,Q2组、Q3组和Q4组的慢性疼痛风险均增加(Q2组:OR=1.365,95%CI:1.149~1.622,P<0.001;Q3组:OR=1.291,95%CI:1.082~1.541,P=0.005;Q4组:OR=1.874,95%CI:1.579~2.224,P<0.001)。模型2中,与Q1组相比,其他3组慢性疼痛风险增加(Q2组:OR=1.359,95%CI:1.137~1.624,P=0.001;Q3组:OR=1.260,95%CI:1.039~1.528,P=0.019;Q4组:OR=1.735,95%CI:1.413~2.132,P<0.001)。模型3中与Q1组相比,Q4组的慢性疼痛风险增加49.2%(OR=1.492,95%CI:1.208~1.842,P<0.001)。在模型2和模型3中,急性疼痛与WWI未见相关性(均P>0.05);3个模型均未发现亚急性疼痛与WWI存在相关性(均P>0.05)。结论:WWI与美国成人急性疼痛、亚急性疼痛之间未见明显相关性,但随着WWI的增加,慢性疼痛风险增加,所以有必要通过大规模前瞻性研究进一步验证这一结论。
文摘目的研究长期规律体育训练青少年的身体组成情况及相关影响因素。方法本研究为前瞻性队列研究,采用便利抽样法选取2023年6月在济宁市体育训练中心接受长期规律体育训练的青少年,并进行体格测量,获取身高、体重、体质量指数(body mass index,BMI)、血压、心率、腰围、臀围等数据,采用问卷调查获取睡眠时间、电子屏幕使用时间和家庭收入情况。2024年6月重复体格测量及问卷调查,并采用生物电阻抗法补充测量人体成分(体成分体重、骨骼肌量、体脂肪量、体脂率)。以随访期体脂率、BMI和腰围作为因变量,以训练类型(直接对抗性训练或非直接对抗性训练)作为自变量建立线性回归模型,分析训练类型与体脂率、BMI及腰围的相关性。结果共纳入110名青少年(女生39名、男生71名),其中65人接受直接对抗性训练,45人接受非直接对抗性训练,中位年龄为13.21(12.46,14.33)岁,超重或肥胖率为27.27%,腰围偏高率为24.55%,血压偏高率为16.36%。随访期超重或肥胖率为24.55%,腰围偏高率为26.36%,血压偏高率为13.64%,其中血压偏高组的腰围(P=0.034)、腰围偏高率(P=0.011)和体成分体重(P=0.049)均显著高于血压正常组。56.36%的青少年随访期BMI较基线期增加,63.64%的青少年随访期腰围较基线期增加。线性回归模型校正性别、年龄、基线期数据、睡眠时间、电子屏幕使用时间和家庭收入情况后显示,直接对抗性运动与体脂率(β=-6.57,95%CI:-9.37~-3.78,P<0.001)、BMI(β=-1.30,95%CI:-2.50~-0.11,P=0.036)、腰围(β=-2.31,95%CI:-4.40~-0.22,P=0.034)均呈显著负相关性。结论进行长期规律体育训练的青少年中,超重或肥胖、腹型肥胖和血压偏高的情况仍较多,应重视这一群体的肥胖情况以及潜在不良影响。此外,体育运动方式可能会影响青少年人体成分变化,直接对抗性运动有助于降低青少年体脂率、BMI和腰围。
文摘目的采用meta分析观察运动对改善超重及肥胖人群神经可塑性的价值。方法检索Web of Science、PubMed、PsycINFO及中国知网自建库至2024年8月1日关于运动干预后超重或肥胖人群脑激活变化的功能MRI(fMRI)文献,采用激活似然估计(ALE)法分析数据,得到脑区激活簇坐标。结果最终共纳入11篇文献,包括186名超重及肥胖受试者。运动后,所有受试者右额中回激活增加,左小脑前叶、左楔前叶及右楔叶激活减少;其中,未成年人右扣带回及右颞上回激活增加,左小脑前叶、双侧中央前回激活减少;成年人双侧豆状核、右额中回、右扣带回及右海马旁回激活增加,双侧楔前叶激活减少。结论运动可改善超重及肥胖人群执行功能、工作记忆、注意力和运动控制相关脑区激活程度,且运动后未成年人与成年人之间改善情况存在差异。
