Aim To investigate the effects of astragaloside IV （ASI） on high-fat diet （HFD） induced obese mice. Methods The male mice aged 6 weeks were randomly divided into three groups （u- 18/group）, namely control group,...Aim To investigate the effects of astragaloside IV （ASI） on high-fat diet （HFD） induced obese mice. Methods The male mice aged 6 weeks were randomly divided into three groups （u- 18/group）, namely control group, model group and ASI-treated group. Control group were fed with standard diet, whereas the other two groups were given high fat diet. ASI-treated mice were daily intraperitoneally injected with ASI （25 nag · kg^-1）. Mean- while, the other group mice were treated with saline. Body weight of mice was monitored every week and lasted for 13 weeks. Serum cholesterol and triglyceride content were measured with respective kits. Serum leptin level was deter- mined by ELISA kit. Expression of leptin receptor in hypothalamus was measured by Western blot assay. Gene ex- pression of neuropeptide Y （NPY） and agouti-related protein （AGRP） in hypothalamus was detected by qPCR assay. In addition, leptin receptor-deficient db/db mice were given intraperitoneally with ASI （25 mg ~ kg-1） or saline for 13 weeks （u- 8/group）. Results ASI blocked body weight gain, suppressed appetite, improved leptin resistance, lowered serum triacylglycerol （TG） and total cholesterol （TC） contents, reduced accumulation of fat tissues and pre- vented enlargement of adipose cells in HFD fed mice. Furthermore, ASI increased the protein expression level of lep- tin receptor in hypothalamus, and inhibited the mRNA expression levels of NPY and AGRP. However, ASI could not decrease body gain in leptin receptor - deficient db/db mice as well as the mRNA expression levels of NPY and AGRP. Conclusion The study suggested that ASI could efficiently prevent HFD-induced obesity in C57BL/6 mice,which was partially mediated through enhancing leptin signaling transduction.展开更多
Objective To investigate the prevalence of hypertension overweight/obesity and the combined effect on the incidence of cardiovascular disease(CVD).Methods The study population(aged from 35 to 64)were selected from 9 r...Objective To investigate the prevalence of hypertension overweight/obesity and the combined effect on the incidence of cardiovascular disease(CVD).Methods The study population(aged from 35 to 64)were selected from 9 regions of China by cluster sampling method.The baseline was conducted in 2010,and the follow-up survey was done in 2017.Participants with 24≤BMI28 kg/m^2 was defined as overweight,BMI≥28 kg/m^2 was defined as obesity.展开更多
Objective To explore the sequential effects of hypoxic exercising on miR-27/PPARγand lipid metabolism targetgene and protein expression levels in the obesity rats’liver.Methods 13-week-old male diet-induced obesity ...Objective To explore the sequential effects of hypoxic exercising on miR-27/PPARγand lipid metabolism targetgene and protein expression levels in the obesity rats’liver.Methods 13-week-old male diet-induced obesity rats were randomlydivided into three groups(n=10):normal oxygen concentration quiet group(N),hypoxia quiet group(H),hypoxic exercise group(HE).Exercise training on the horizontal animal treadmill for 1 h/d,5 d/week for a total of 4 week,and the intensity of horizontaltreadmill training was 20 m/min(hypoxic concentration was 13.6%).Comparison of the weights of perirenal fat and epididymal fat in rats across different groups and calculation of Lee’s index based on body weight and body length of rats in each group were done.And the serum concentrations of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high-densitylipoprotein cholesterol(HDL-C)levels were detected.RT-PCR and Western Blot were used to detect the levels of miR-27,PPARγ,CYP7A1 and CD36.Results Hypoxic exercise decreased the expression levels of miR-27 in the obese rats’liver,however,theexpression level of PPARγwas gradually increased.The expression levels of miR-27 in HE group were significantly lower than Ngroup(P<0.05).The expression levels of PPARγmRNA in N group were significantly lower than H group(P<0.05),especially lowerthan HE group(P<0.01).The protein expression of PPARγprotein in N group was significantly lower than that other groups(P<0.01).The expression of lipid metabolism-related genes and proteins increased in the obese rats’liver.The expression of CYP7A1mRNA in N group was significantly lower than H group(P<0.05),especially lower than HE group(P<0.01).The expression ofCYP7A1 protein in the obese rats’liver in N group was extremely lower than H group and HE group(P<0.01).The proteinexpression of CD36 in N group was significantly lower than that in HE group(P<0.05).Hypoxia exercise improved the relatedphysiological and biochemical indexes of lipid metabolism disorder.The perirenal fat weight of obese rats in HE group wasextremely lower than N group and H group(P<0.01),and the perirenal fat weight in N group was significantly higher than H group(P<0.05).The epididymal fat weight in N group was significantly higher than H group(P<0.05),and extremely higher than HEgroup(P<0.01).The Lee’s index in HE group was extremely lower than N group and H group(P<0.01).The serum concentration ofTC in obese rats in HE group was extremely lower than N group and H group(P<0.01).The serum concentration of TG in HE groupwas extremely lower than N group and H group(P<0.01).The serum concentration of LDL-C in N group was extremely higher thanHE group(P<0.01).The serum concentration of HDL-C in N group was extremely lower than H group(P<0.01).Conclusion Hypoxiaand hypoxia exercise may negatively regulate the levels of PPARγby inhibiting miR-27 in the obese rats’liver,thereby affecting theexpression of downstream target genes CYP7A1 and CD36,and promoting cholesterol,fatty acid oxidation and HDL-C transport inthe liver,and ultimately the lipid levels in obese rats were improved.The effect of hypoxia exercise on improving blood lipid isbetter than simple hypoxia intervention.展开更多
FTO基因（fat mass and obesity associated gene）为人类肥胖基因,2007年,由英国牛津大学Mark I.McC arthy博士发现。该基因位于人16号染色体,其突变与人类肥胖发生密切相关。英国人群数据显示：与正常人相比,FTO基因双拷贝变异携带者...FTO基因（fat mass and obesity associated gene）为人类肥胖基因,2007年,由英国牛津大学Mark I.McC arthy博士发现。该基因位于人16号染色体,其突变与人类肥胖发生密切相关。英国人群数据显示：与正常人相比,FTO基因双拷贝变异携带者,肥胖几率约高70%,Ⅱ型糖尿病风险约高50%;FTO基因单拷贝变异携带者,肥胖几率约高30%,Ⅱ型糖尿病风险约高25%。展开更多
Aim As we all know, apelin acts as the endogenous ligand of APJ, being a member of G protein cou- pled receptors family, apelin/APJ system is involved in plentiful diseases and extremely responsible for the occur- ren...Aim As we all know, apelin acts as the endogenous ligand of APJ, being a member of G protein cou- pled receptors family, apelin/APJ system is involved in plentiful diseases and extremely responsible for the occur- rence and the development of cardiovascular diseases, among many kinds of heart diseases, it is the cardiac hyper- trophy that catches our attention. The myocardial expression of apelin/APJ decreased in rats with left ventrieular hypertrophy suggesting us there is a link between apelin and cardiac hypertrophy. Furthermore, it has been repor- ted that apelin is able to alleviate cardiac hypertrophy induced by Ang II, H202 and exercise. Nevertheless, our la- boratory discovered that apelin is certain to induce cardiac hypertrophy through PI3k-Akt-ERK1/2-p70S6K pathway or via up-regulating the levels of ROS to cause oxidative stress. The above-mentioned contradiction indicates us apelin may have dual effects in cardiac hypertrophy. Moreover, we also illuminate that apelin is involved in some diseases such as obesity, diabetes, hypertension, myoearditis and myocardial infarction, eoineidentally, all these diseases are associated with cardiac hypertrophy. Therefore, this review is aim to unveil the intricate relationship between apelin and cardiac hypertrophy.展开更多
OBJECTIVE To investigate the effect of Pandanus amaryllifolius leaf in high-fat diet-induced insulin resistance in mice model.METHODS To induce obesity,male ICR mice were fed with a high-fat diet(45%fat)for six weeks....OBJECTIVE To investigate the effect of Pandanus amaryllifolius leaf in high-fat diet-induced insulin resistance in mice model.METHODS To induce obesity,male ICR mice were fed with a high-fat diet(45%fat)for six weeks.The mice were divided into four groups(n=8):non-obese control mice were treated with 5% gum arabic and obese mice were treated with Pandanus amaryllifolius(125and 250mg·kg-1·d-1),or 5% gum arabic.After six weeks of treatments,the fasting blood glucose,serum insulin,OGTT and fat cell protein expression of glucose transporter 4(GLUT4)were determined.RESULTS Administration of Pandanus amaryllifolius showed significantly(P<0.05)reduced the high blood glucose,inhibited the abnormal increase in blood glucose level during OGTT,and decreased the high level of serum insulin.Moreover,it is interesting that the protein expression of GLUT4 was effectively increased by Pandanus amaryllifolius.CONCLUSION These findings demonstrate that the extract from Pandanus amaryllifolius leaf possesses antihyperglycemic action in obese mice by improving insulin sensitivity and stimulating GLUT4 expression in adipose tissue.展开更多
Type 2 diabetes and obesity in young adults青年2型糖尿病和肥胖·Glucose-lowering medicines for type 2 diabetes2型糖尿病降血糖药物·The introduction of insulin in type 2 diabetes mellitus2型糖尿病胰岛素介绍...Type 2 diabetes and obesity in young adults青年2型糖尿病和肥胖·Glucose-lowering medicines for type 2 diabetes2型糖尿病降血糖药物·The introduction of insulin in type 2 diabetes mellitus2型糖尿病胰岛素介绍·Continuous glucose monitoring and pumps持续的血糖监测和抽送·Diet and diabetes饮食和糖尿病·Residential aged care facility residents:training issues for Australian general展开更多
TLR2-deficiency promotes prenatal LPS exposure-induced offspring hyperlipidemia,Toll-like receptor 2(TLR2),which recognizes several lipopeptides and transduces inflammatory signaling,promotes the pathogenesis of diet-...TLR2-deficiency promotes prenatal LPS exposure-induced offspring hyperlipidemia,Toll-like receptor 2(TLR2),which recognizes several lipopeptides and transduces inflammatory signaling,promotes the pathogenesis of diet-induced dyslipidemia and obesity.TLR2-deficient mice were shown to have improved insulin sensitivity and reduced diet-induced metabolic syndrome.Previous studies demonstrated that prenatal lipopolysaccharide(LPS)exposure causes dyslipidemia accompanied by increased body weight and insulin resistance in offspring.展开更多
2013年12月30日,哈佛大学医学院宋威博士应邀访问西南大学家蚕基因组生物学国家重点实验室,并为师生做了题为＂Activin signaling mediates muscle-to-adipose communication in a mitochondria dysfunction-mediated obesity model＂...2013年12月30日,哈佛大学医学院宋威博士应邀访问西南大学家蚕基因组生物学国家重点实验室,并为师生做了题为＂Activin signaling mediates muscle-to-adipose communication in a mitochondria dysfunction-mediated obesity model＂的精彩学术报告,重点介绍了果蝇肌肉中线粒体功能紊乱导致脂肪体中的线粒体功能也发生改变,并提出了肥胖形成过程中不同组织间线粒体功能的同步化模型。展开更多
文摘Aim To investigate the effects of astragaloside IV （ASI） on high-fat diet （HFD） induced obese mice. Methods The male mice aged 6 weeks were randomly divided into three groups （u- 18/group）, namely control group, model group and ASI-treated group. Control group were fed with standard diet, whereas the other two groups were given high fat diet. ASI-treated mice were daily intraperitoneally injected with ASI （25 nag · kg^-1）. Mean- while, the other group mice were treated with saline. Body weight of mice was monitored every week and lasted for 13 weeks. Serum cholesterol and triglyceride content were measured with respective kits. Serum leptin level was deter- mined by ELISA kit. Expression of leptin receptor in hypothalamus was measured by Western blot assay. Gene ex- pression of neuropeptide Y （NPY） and agouti-related protein （AGRP） in hypothalamus was detected by qPCR assay. In addition, leptin receptor-deficient db/db mice were given intraperitoneally with ASI （25 mg ~ kg-1） or saline for 13 weeks （u- 8/group）. Results ASI blocked body weight gain, suppressed appetite, improved leptin resistance, lowered serum triacylglycerol （TG） and total cholesterol （TC） contents, reduced accumulation of fat tissues and pre- vented enlargement of adipose cells in HFD fed mice. Furthermore, ASI increased the protein expression level of lep- tin receptor in hypothalamus, and inhibited the mRNA expression levels of NPY and AGRP. However, ASI could not decrease body gain in leptin receptor - deficient db/db mice as well as the mRNA expression levels of NPY and AGRP. Conclusion The study suggested that ASI could efficiently prevent HFD-induced obesity in C57BL/6 mice,which was partially mediated through enhancing leptin signaling transduction.
文摘Objective To investigate the prevalence of hypertension overweight/obesity and the combined effect on the incidence of cardiovascular disease(CVD).Methods The study population(aged from 35 to 64)were selected from 9 regions of China by cluster sampling method.The baseline was conducted in 2010,and the follow-up survey was done in 2017.Participants with 24≤BMI28 kg/m^2 was defined as overweight,BMI≥28 kg/m^2 was defined as obesity.
文摘Objective To explore the sequential effects of hypoxic exercising on miR-27/PPARγand lipid metabolism targetgene and protein expression levels in the obesity rats’liver.Methods 13-week-old male diet-induced obesity rats were randomlydivided into three groups(n=10):normal oxygen concentration quiet group(N),hypoxia quiet group(H),hypoxic exercise group(HE).Exercise training on the horizontal animal treadmill for 1 h/d,5 d/week for a total of 4 week,and the intensity of horizontaltreadmill training was 20 m/min(hypoxic concentration was 13.6%).Comparison of the weights of perirenal fat and epididymal fat in rats across different groups and calculation of Lee’s index based on body weight and body length of rats in each group were done.And the serum concentrations of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high-densitylipoprotein cholesterol(HDL-C)levels were detected.RT-PCR and Western Blot were used to detect the levels of miR-27,PPARγ,CYP7A1 and CD36.Results Hypoxic exercise decreased the expression levels of miR-27 in the obese rats’liver,however,theexpression level of PPARγwas gradually increased.The expression levels of miR-27 in HE group were significantly lower than Ngroup(P<0.05).The expression levels of PPARγmRNA in N group were significantly lower than H group(P<0.05),especially lowerthan HE group(P<0.01).The protein expression of PPARγprotein in N group was significantly lower than that other groups(P<0.01).The expression of lipid metabolism-related genes and proteins increased in the obese rats’liver.The expression of CYP7A1mRNA in N group was significantly lower than H group(P<0.05),especially lower than HE group(P<0.01).The expression ofCYP7A1 protein in the obese rats’liver in N group was extremely lower than H group and HE group(P<0.01).The proteinexpression of CD36 in N group was significantly lower than that in HE group(P<0.05).Hypoxia exercise improved the relatedphysiological and biochemical indexes of lipid metabolism disorder.The perirenal fat weight of obese rats in HE group wasextremely lower than N group and H group(P<0.01),and the perirenal fat weight in N group was significantly higher than H group(P<0.05).The epididymal fat weight in N group was significantly higher than H group(P<0.05),and extremely higher than HEgroup(P<0.01).The Lee’s index in HE group was extremely lower than N group and H group(P<0.01).The serum concentration ofTC in obese rats in HE group was extremely lower than N group and H group(P<0.01).The serum concentration of TG in HE groupwas extremely lower than N group and H group(P<0.01).The serum concentration of LDL-C in N group was extremely higher thanHE group(P<0.01).The serum concentration of HDL-C in N group was extremely lower than H group(P<0.01).Conclusion Hypoxiaand hypoxia exercise may negatively regulate the levels of PPARγby inhibiting miR-27 in the obese rats’liver,thereby affecting theexpression of downstream target genes CYP7A1 and CD36,and promoting cholesterol,fatty acid oxidation and HDL-C transport inthe liver,and ultimately the lipid levels in obese rats were improved.The effect of hypoxia exercise on improving blood lipid isbetter than simple hypoxia intervention.
文摘FTO基因（fat mass and obesity associated gene）为人类肥胖基因,2007年,由英国牛津大学Mark I.McC arthy博士发现。该基因位于人16号染色体,其突变与人类肥胖发生密切相关。英国人群数据显示：与正常人相比,FTO基因双拷贝变异携带者,肥胖几率约高70%,Ⅱ型糖尿病风险约高50%;FTO基因单拷贝变异携带者,肥胖几率约高30%,Ⅱ型糖尿病风险约高25%。
文摘Aim As we all know, apelin acts as the endogenous ligand of APJ, being a member of G protein cou- pled receptors family, apelin/APJ system is involved in plentiful diseases and extremely responsible for the occur- rence and the development of cardiovascular diseases, among many kinds of heart diseases, it is the cardiac hyper- trophy that catches our attention. The myocardial expression of apelin/APJ decreased in rats with left ventrieular hypertrophy suggesting us there is a link between apelin and cardiac hypertrophy. Furthermore, it has been repor- ted that apelin is able to alleviate cardiac hypertrophy induced by Ang II, H202 and exercise. Nevertheless, our la- boratory discovered that apelin is certain to induce cardiac hypertrophy through PI3k-Akt-ERK1/2-p70S6K pathway or via up-regulating the levels of ROS to cause oxidative stress. The above-mentioned contradiction indicates us apelin may have dual effects in cardiac hypertrophy. Moreover, we also illuminate that apelin is involved in some diseases such as obesity, diabetes, hypertension, myoearditis and myocardial infarction, eoineidentally, all these diseases are associated with cardiac hypertrophy. Therefore, this review is aim to unveil the intricate relationship between apelin and cardiac hypertrophy.
基金The project supported by Faculty of Medicine,Thammasat University Thailand
文摘OBJECTIVE To investigate the effect of Pandanus amaryllifolius leaf in high-fat diet-induced insulin resistance in mice model.METHODS To induce obesity,male ICR mice were fed with a high-fat diet(45%fat)for six weeks.The mice were divided into four groups(n=8):non-obese control mice were treated with 5% gum arabic and obese mice were treated with Pandanus amaryllifolius(125and 250mg·kg-1·d-1),or 5% gum arabic.After six weeks of treatments,the fasting blood glucose,serum insulin,OGTT and fat cell protein expression of glucose transporter 4(GLUT4)were determined.RESULTS Administration of Pandanus amaryllifolius showed significantly(P<0.05)reduced the high blood glucose,inhibited the abnormal increase in blood glucose level during OGTT,and decreased the high level of serum insulin.Moreover,it is interesting that the protein expression of GLUT4 was effectively increased by Pandanus amaryllifolius.CONCLUSION These findings demonstrate that the extract from Pandanus amaryllifolius leaf possesses antihyperglycemic action in obese mice by improving insulin sensitivity and stimulating GLUT4 expression in adipose tissue.
文摘Type 2 diabetes and obesity in young adults青年2型糖尿病和肥胖·Glucose-lowering medicines for type 2 diabetes2型糖尿病降血糖药物·The introduction of insulin in type 2 diabetes mellitus2型糖尿病胰岛素介绍·Continuous glucose monitoring and pumps持续的血糖监测和抽送·Diet and diabetes饮食和糖尿病·Residential aged care facility residents:training issues for Australian general
文摘TLR2-deficiency promotes prenatal LPS exposure-induced offspring hyperlipidemia,Toll-like receptor 2(TLR2),which recognizes several lipopeptides and transduces inflammatory signaling,promotes the pathogenesis of diet-induced dyslipidemia and obesity.TLR2-deficient mice were shown to have improved insulin sensitivity and reduced diet-induced metabolic syndrome.Previous studies demonstrated that prenatal lipopolysaccharide(LPS)exposure causes dyslipidemia accompanied by increased body weight and insulin resistance in offspring.
文摘2013年12月30日,哈佛大学医学院宋威博士应邀访问西南大学家蚕基因组生物学国家重点实验室,并为师生做了题为＂Activin signaling mediates muscle-to-adipose communication in a mitochondria dysfunction-mediated obesity model＂的精彩学术报告,重点介绍了果蝇肌肉中线粒体功能紊乱导致脂肪体中的线粒体功能也发生改变,并提出了肥胖形成过程中不同组织间线粒体功能的同步化模型。
