A simplified approach was proposed to analyze the negative skin friction calculation of special-shaped pile considering pile-soil interaction under surcharge. Based on the concentric cylinder shearing theory, consider...A simplified approach was proposed to analyze the negative skin friction calculation of special-shaped pile considering pile-soil interaction under surcharge. Based on the concentric cylinder shearing theory, considering the changes of pile shape(such as, taper angle and diameters of pile base, etc.), the load-transfer of special-shaped pile was built. The accuracy of the developed simplified approach was verified by numerical simulation model with the same condition. Then, the influence factors, such as, taper angles, the diameter of pile base, surcharge, and pile-soil interface parameters were analyzed and discussed. The results show that the developed simplified approach can calculate NSF of special-shaped pile under surcharge effectively. A limited parametric study indicates that in many practical situations special-shaped piles(such as belled wedge pile shown in this work) offer a design option that is more economical than traditional uniform cross-section piles.展开更多
OBJECTIVE To discover a small molecule targeting ULK1-modulated cell death of triple negative breast cancer and exploreits potential mechanisms.METHODS ULK1 expression was analyzed by The Cancer Genome Atlas(TCGA)anal...OBJECTIVE To discover a small molecule targeting ULK1-modulated cell death of triple negative breast cancer and exploreits potential mechanisms.METHODS ULK1 expression was analyzed by The Cancer Genome Atlas(TCGA)analysis and tissue microarray(TMA)analysis.ULK1agonist was designed by using in silico screening,as well as modified by chemical synthesis and screened by kinase and anti-proliferative activities.The amino acid residues that key to the activation site of LYN-1604 were determined by site-directed mutagenesis,as well as in vitro kinase assay and ADP-Glo kinase assay.The mechanisms of LYN-1604 induced cell death were investigated by fluorescence microscope,western blotting,flow cytometry analysis,immunocytochemistry,as well as si RNA and GFP-m RFP-LC3 plasmid transfections.Potential ULK1 interactors were discovered by performing comparative microarray analysis and the therapeutic effect of LYN-1604 was assessed by xenograft breast cancer mouse model.RESULTS We found that ULK1 was remarkably downregulated in breast cancer tissue samples,especial y in triple negative breast cancer(TNBC).32 candidate smal molecules were synthesized,and we discovered a small molecule named LYN-1604 as the best candidate ULK1agonist.Additionally,we identified that three amino acid residues(LYS50,LEU53 and TYR89)were key to the activation site of LYN-1604 and ULK1.Subsequently,we demonstrated that LYN-1604 could induce autophagy-associated cell death via ULK complex(ULK1-m ATG13-FIP200-ATG101)in MDA-MB-231 cells.We also found that LYN-1604 induced cell death involved in ATF3,RAD21 and caspase 3,accompanied with autophagy and apoptosis.Moreover,we demonstrated that LYN-1604 had a good therapeutic potential on TNBC by targeting ULK1-modulated cell death in vivo.CONCLUSION We discovered a small molecule(LYN-1604)has therapeutic potential by targeting ULK1-modulated cell death associated with autophagy and apoptosis of TNBC in vitro and in vivo,which could be utilized as a new anti-TNBC drug candidate.展开更多
By using the first-principle calculations and nonequilibrium Green functions method, the electronic transport properties of molecular devices constructed by C82, C80BN and C80N2 were studied. The results show that the...By using the first-principle calculations and nonequilibrium Green functions method, the electronic transport properties of molecular devices constructed by C82, C80BN and C80N2 were studied. The results show that the electronic transport properties of molecular devices are affected by doped atoms. Negative differential resistance (NDR) behavior can be observed in certain bias regions for C82 and C80BN molecular devices but cannot be observed for C80N2 molecular device. A mechanism for the negative differential resistance behavior was suggested.展开更多
In May 2018,the primetime news casted a shocking report saying that radon concentration on a certain model of bed mattress found to be as high as 2200 Bq/m3.After a humble,the Nuclear Safety and Security Commission(NS...In May 2018,the primetime news casted a shocking report saying that radon concentration on a certain model of bed mattress found to be as high as 2200 Bq/m3.After a humble,the Nuclear Safety and Security Commission(NSSC)of Korea confirmed that significant amount of thoron gas is emanated from several mattress models marketed by a company claiming beneficial health effects of negative ions.Laboratory analysis showed that some internal fabric sheets of those mattresses contain high concentration of Th-232.It was revealed that the manufacture treated the material with so-called‘negative ion powder’procured from the market and NSSC found that its radioactive content is the monazite powder.Although measurements with reliable instruments resulted in somewhat lower values,the tentative but conservative estimates of doses to the users are still remarkable,ranging from a few to 14 mSv a year.Most of the affected models have been marketed from 2010 but earlier models,with lower thorium content,were supplied from 2006.As many as 88,000 mattresses have been produced.The manufacturer with help of the government,recalled all the affected models and separated the radioactive internals.A large amount of waste is waiting for the government decision on disposal method.Similar problems were identified in other consumer products including latex mattresses and pillows imported,hot pads,and several models of sanitary or health-aid goods.These episodes called for revisiting NORM control strategy in Korea.展开更多
In order to analyze the energy consumption of wheel loader during working process, a three-dimensional（3D） model of wheel loader is established by Pro/E software, and it is imported into the software SimulationX tob...In order to analyze the energy consumption of wheel loader during working process, a three-dimensional（3D） model of wheel loader is established by Pro/E software, and it is imported into the software SimulationX tobuild the kinetic model of the whole wheel loader. Meanwhile, the simulation model of hydraulic system is builtby the same software. By integrating the simulation model of hydraulic system with the kinetic model, the co-sim-ulation model of wheel loader could be achieved. The precision of co-simulation model of the working device isverified through the comparison between simulation results and test results. The testing results show that, undernegative loading condition, the energy loss in multi-way valve accounts for about 52%. The important energy lossin loader＇s working hydraulic system using constant displacement pump is the large throttling loss of multi-wayvalves. This study provides a reference for designing of a more energy-efficient hydaulic system of wheel loader.展开更多
Anodic oxidation with different electrolyte was employed to improve the electrochemical properties of carbon paper as negative electrode for vanadium redox battery(VRB).The treated carbon paper exhibits enhanced elect...Anodic oxidation with different electrolyte was employed to improve the electrochemical properties of carbon paper as negative electrode for vanadium redox battery(VRB).The treated carbon paper exhibits enhanced electrochemical activity for V^2+/V^3+redox reaction.The sample(CP-NH3)treated in NH3 solution demonstrates superior performance in comparison with the sample(CP-NaOH)treated in NaOH solution.X-ray photoelectron spectroscopy results show that oxygen-and nitrogen-containing functional groups have been introduced on CP-NH3 surface by the treatment,and Raman spectra confirm the increased surface defect of CP-NH3.Energy storage performance of cell was evaluated by charge/discharge measurement by using CP-NH3.Usage of CP-NH3 can greatly improve the cell performance with energy efficiency increase of 4.8%at 60 mA/cm^2.The excellent performance of CP-NH3 mainly results from introduction of functional groups as active sites and improved wetting properties.This work reveals that anodic oxidation is a clean,simple,and efficient method for boosting the performance of carbon paper as negative electrode for VRB.展开更多
In this paper a negative electron affinity （NEA） multialkali photocathode of （Na<sub>2</sub>KSb-Cs）-O-Cs structure is fabricated by new technology. It is found that its emission stability is much bette...In this paper a negative electron affinity （NEA） multialkali photocathode of （Na<sub>2</sub>KSb-Cs）-O-Cs structure is fabricated by new technology. It is found that its emission stability is much better than that of the NEA GaAs photocathode, but is inferior to that of the conventional Na<sub>2</sub>KSb（Cs）. After 70 hour performance in a pumping vacuum system, the emission sensitivity of the NEA （Na<sub>2</sub>KSb-Cs）-O-Cs photocathode drops only by 2.5%. The emission stability is closely related to the states of the activation cesium and oxygen during activation, best results being obtained with cesium ions and excited oxygen. Furthermore, better photoemission sensitivity and emission stability may be obtained if the cathode is illuminated by intense white light during the activation process. The performance of the NEA （Na<sub>2</sub>KSb-Cs）-O-Cs cathode which has not been illuminated by intense white light during activation may be improved by the illumination even during operation intermission.展开更多
Actin cytoskeleton plays an important role in cell morphogenesis in plants as demonstrated by pharmacological,biochemical,and genetic studies.The actin cytoskeleton may be involved in
This study presents a significant advancement in the vibration analysis of functionally graded sandwich plates with auxetic cores by introducing a general viscoelastic foundation model that more accurately reflects th...This study presents a significant advancement in the vibration analysis of functionally graded sandwich plates with auxetic cores by introducing a general viscoelastic foundation model that more accurately reflects the complex interactions between the plate and the foundation.The novelty of this study is that the proposed viscoelastic foundation model incorporates elastic and damping effects in both the Winkler and Pasternak layers.To develop the theoretical framework for this analysis,the higher-order shear deformation theory is employed,while Hamilton's principle is used to derive the governing equations of motion.The closed-form solution is used to determine the damped vibration behaviors of the sandwich plates.The precision and robustness of the proposed mathematical model are validated through several comparison studies with existing numerical results.A detailed parametric study is conducted to investigate the influence of various parameters,including the elastic and damping coefficients of the foundation,the material gradation,and the properties of the auxetic core on the vibration behavior of the plates.The numerical results provide new insights into the vibration characteristics of sandwich plates with auxetic cores resting on viscoelastic foundation,highlighting the significant role of the two damping coefficients and auxetic cores in the visco-vibration behavior of the plates.展开更多
基金Projects(51278170,U1134207)supported by the National Natural Science Foundation of ChinaProject(B13024)supported by the Program of Introducing Talents of Discipline to Universities,China
文摘A simplified approach was proposed to analyze the negative skin friction calculation of special-shaped pile considering pile-soil interaction under surcharge. Based on the concentric cylinder shearing theory, considering the changes of pile shape(such as, taper angle and diameters of pile base, etc.), the load-transfer of special-shaped pile was built. The accuracy of the developed simplified approach was verified by numerical simulation model with the same condition. Then, the influence factors, such as, taper angles, the diameter of pile base, surcharge, and pile-soil interface parameters were analyzed and discussed. The results show that the developed simplified approach can calculate NSF of special-shaped pile under surcharge effectively. A limited parametric study indicates that in many practical situations special-shaped piles(such as belled wedge pile shown in this work) offer a design option that is more economical than traditional uniform cross-section piles.
基金supported by National Natural Science Foundation of China(81402496,81673455and 81602627)China Postdoctoral Special Science Foundation(2017T100704)China Postdoctoral Science Foundation(2015M580794)
文摘OBJECTIVE To discover a small molecule targeting ULK1-modulated cell death of triple negative breast cancer and exploreits potential mechanisms.METHODS ULK1 expression was analyzed by The Cancer Genome Atlas(TCGA)analysis and tissue microarray(TMA)analysis.ULK1agonist was designed by using in silico screening,as well as modified by chemical synthesis and screened by kinase and anti-proliferative activities.The amino acid residues that key to the activation site of LYN-1604 were determined by site-directed mutagenesis,as well as in vitro kinase assay and ADP-Glo kinase assay.The mechanisms of LYN-1604 induced cell death were investigated by fluorescence microscope,western blotting,flow cytometry analysis,immunocytochemistry,as well as si RNA and GFP-m RFP-LC3 plasmid transfections.Potential ULK1 interactors were discovered by performing comparative microarray analysis and the therapeutic effect of LYN-1604 was assessed by xenograft breast cancer mouse model.RESULTS We found that ULK1 was remarkably downregulated in breast cancer tissue samples,especial y in triple negative breast cancer(TNBC).32 candidate smal molecules were synthesized,and we discovered a small molecule named LYN-1604 as the best candidate ULK1agonist.Additionally,we identified that three amino acid residues(LYS50,LEU53 and TYR89)were key to the activation site of LYN-1604 and ULK1.Subsequently,we demonstrated that LYN-1604 could induce autophagy-associated cell death via ULK complex(ULK1-m ATG13-FIP200-ATG101)in MDA-MB-231 cells.We also found that LYN-1604 induced cell death involved in ATF3,RAD21 and caspase 3,accompanied with autophagy and apoptosis.Moreover,we demonstrated that LYN-1604 had a good therapeutic potential on TNBC by targeting ULK1-modulated cell death in vivo.CONCLUSION We discovered a small molecule(LYN-1604)has therapeutic potential by targeting ULK1-modulated cell death associated with autophagy and apoptosis of TNBC in vitro and in vivo,which could be utilized as a new anti-TNBC drug candidate.
基金Project(50721003)supported by the National Natural Science Foundation of ChinaProject(10C1171)supported by the Scientific Research Fund of Hunan Provincial Education Department,ChinaProject(11JJ3073)supported by the Natural Science Foundation of Hunan Province,China
文摘By using the first-principle calculations and nonequilibrium Green functions method, the electronic transport properties of molecular devices constructed by C82, C80BN and C80N2 were studied. The results show that the electronic transport properties of molecular devices are affected by doped atoms. Negative differential resistance (NDR) behavior can be observed in certain bias regions for C82 and C80BN molecular devices but cannot be observed for C80N2 molecular device. A mechanism for the negative differential resistance behavior was suggested.
文摘In May 2018,the primetime news casted a shocking report saying that radon concentration on a certain model of bed mattress found to be as high as 2200 Bq/m3.After a humble,the Nuclear Safety and Security Commission(NSSC)of Korea confirmed that significant amount of thoron gas is emanated from several mattress models marketed by a company claiming beneficial health effects of negative ions.Laboratory analysis showed that some internal fabric sheets of those mattresses contain high concentration of Th-232.It was revealed that the manufacture treated the material with so-called‘negative ion powder’procured from the market and NSSC found that its radioactive content is the monazite powder.Although measurements with reliable instruments resulted in somewhat lower values,the tentative but conservative estimates of doses to the users are still remarkable,ranging from a few to 14 mSv a year.Most of the affected models have been marketed from 2010 but earlier models,with lower thorium content,were supplied from 2006.As many as 88,000 mattresses have been produced.The manufacturer with help of the government,recalled all the affected models and separated the radioactive internals.A large amount of waste is waiting for the government decision on disposal method.Similar problems were identified in other consumer products including latex mattresses and pillows imported,hot pads,and several models of sanitary or health-aid goods.These episodes called for revisiting NORM control strategy in Korea.
文摘In order to analyze the energy consumption of wheel loader during working process, a three-dimensional（3D） model of wheel loader is established by Pro/E software, and it is imported into the software SimulationX tobuild the kinetic model of the whole wheel loader. Meanwhile, the simulation model of hydraulic system is builtby the same software. By integrating the simulation model of hydraulic system with the kinetic model, the co-sim-ulation model of wheel loader could be achieved. The precision of co-simulation model of the working device isverified through the comparison between simulation results and test results. The testing results show that, undernegative loading condition, the energy loss in multi-way valve accounts for about 52%. The important energy lossin loader＇s working hydraulic system using constant displacement pump is the large throttling loss of multi-wayvalves. This study provides a reference for designing of a more energy-efficient hydaulic system of wheel loader.
基金Project(NCET-10-0946)supported by Program for New Century Excellent Talents in University of ChinaProject(2017JY0038)supported by Science and Technology Key Project of Sichuan Province,ChinaProject(2013TX8)supported by Titanium and Titanium Alloy Innovation Team of Panzhihua City,China
文摘Anodic oxidation with different electrolyte was employed to improve the electrochemical properties of carbon paper as negative electrode for vanadium redox battery(VRB).The treated carbon paper exhibits enhanced electrochemical activity for V^2+/V^3+redox reaction.The sample(CP-NH3)treated in NH3 solution demonstrates superior performance in comparison with the sample(CP-NaOH)treated in NaOH solution.X-ray photoelectron spectroscopy results show that oxygen-and nitrogen-containing functional groups have been introduced on CP-NH3 surface by the treatment,and Raman spectra confirm the increased surface defect of CP-NH3.Energy storage performance of cell was evaluated by charge/discharge measurement by using CP-NH3.Usage of CP-NH3 can greatly improve the cell performance with energy efficiency increase of 4.8%at 60 mA/cm^2.The excellent performance of CP-NH3 mainly results from introduction of functional groups as active sites and improved wetting properties.This work reveals that anodic oxidation is a clean,simple,and efficient method for boosting the performance of carbon paper as negative electrode for VRB.
基金This work is supported by the Natural Science Foundation of Fujian Province, China.
文摘In this paper a negative electron affinity （NEA） multialkali photocathode of （Na<sub>2</sub>KSb-Cs）-O-Cs structure is fabricated by new technology. It is found that its emission stability is much better than that of the NEA GaAs photocathode, but is inferior to that of the conventional Na<sub>2</sub>KSb（Cs）. After 70 hour performance in a pumping vacuum system, the emission sensitivity of the NEA （Na<sub>2</sub>KSb-Cs）-O-Cs photocathode drops only by 2.5%. The emission stability is closely related to the states of the activation cesium and oxygen during activation, best results being obtained with cesium ions and excited oxygen. Furthermore, better photoemission sensitivity and emission stability may be obtained if the cathode is illuminated by intense white light during the activation process. The performance of the NEA （Na<sub>2</sub>KSb-Cs）-O-Cs cathode which has not been illuminated by intense white light during activation may be improved by the illumination even during operation intermission.
文摘Actin cytoskeleton plays an important role in cell morphogenesis in plants as demonstrated by pharmacological,biochemical,and genetic studies.The actin cytoskeleton may be involved in
基金the funding of the Deanship of Graduate Studies and Scientific Research,Jazan University,Saudi Arabia,through project number:RG24-M027.
文摘This study presents a significant advancement in the vibration analysis of functionally graded sandwich plates with auxetic cores by introducing a general viscoelastic foundation model that more accurately reflects the complex interactions between the plate and the foundation.The novelty of this study is that the proposed viscoelastic foundation model incorporates elastic and damping effects in both the Winkler and Pasternak layers.To develop the theoretical framework for this analysis,the higher-order shear deformation theory is employed,while Hamilton's principle is used to derive the governing equations of motion.The closed-form solution is used to determine the damped vibration behaviors of the sandwich plates.The precision and robustness of the proposed mathematical model are validated through several comparison studies with existing numerical results.A detailed parametric study is conducted to investigate the influence of various parameters,including the elastic and damping coefficients of the foundation,the material gradation,and the properties of the auxetic core on the vibration behavior of the plates.The numerical results provide new insights into the vibration characteristics of sandwich plates with auxetic cores resting on viscoelastic foundation,highlighting the significant role of the two damping coefficients and auxetic cores in the visco-vibration behavior of the plates.
