Objective:To study regulation of external leukemia inhibitory factor(LIF) and interlukin 1(IL 1) on the expression of integrin β 3 in the mouse endometrium during peri implantation period and explore the mechanism wh...Objective:To study regulation of external leukemia inhibitory factor(LIF) and interlukin 1(IL 1) on the expression of integrin β 3 in the mouse endometrium during peri implantation period and explore the mechanism which LIF participate in embryonic implantation.Methods:The mice were injected peritoneally with LIF,IL 1 or IL 1ra at different administration on day 2～4 of pregnancy.The level of integrin β 3 protein was measured with Westernblot method and mRNA measured with RT PCR.Results:The levels of integrin β 3 protein and mRNA were increased by LIF and IL 1(P<0.05) and which relied on administration.The level of integrin β 3 mRNA was decreased by IL 1ra (P<0.05).Conclusion:LIF and IL 1 of cytokines can improve the expression of integrin β 3 in the peri implantation endometrium of mouse.Both of them may take part in implantation by regulating cell adhesion molecules.展开更多
【目的】研究NT3对早期卵泡存活、发育、激素合成的影响及其可能的作用机制,同时探讨NT3和LIF在早期卵泡启动发育方面是否存在协同或叠加效应。【方法】分离新生4d大鼠卵巢随机分配至新鲜组、基础培养组、NT3组、LIF组和NT3+LIF组,在37...【目的】研究NT3对早期卵泡存活、发育、激素合成的影响及其可能的作用机制,同时探讨NT3和LIF在早期卵泡启动发育方面是否存在协同或叠加效应。【方法】分离新生4d大鼠卵巢随机分配至新鲜组、基础培养组、NT3组、LIF组和NT3+LIF组,在37℃培养箱中培养14 d,经固定、包埋、切片后进行组织形态学、PCNA免疫组化、TUNEL凋亡分析,RTPCR检测KL m RNA表达及检测培养液中激素水平。【结果】相比新鲜组和基础培养组,NT3组、LIF组、NT3+LIF组中的始基卵泡比例下降,生长卵泡比例升高,同时PCNA蛋白表达显著增加;TUNEL分析显示NT3组、LIF组、NT3+LIF组的凋亡指数均显著降低;NT3、LIF组、NT3+LIF组卵巢中KL m RNA的表达量分别是对照组的(3.38±0.43)倍、(2.02±0.20)倍、(2.07±0.21)倍。【结论】NT3在早期卵泡存活、发育中有促进作用,上调KL基因表达可能是NT3的作用机制之一。NT3和LIF单独或相加均能促进卵泡发育,但两者之间无明显协同或叠加效应。展开更多
文摘Objective:To study regulation of external leukemia inhibitory factor(LIF) and interlukin 1(IL 1) on the expression of integrin β 3 in the mouse endometrium during peri implantation period and explore the mechanism which LIF participate in embryonic implantation.Methods:The mice were injected peritoneally with LIF,IL 1 or IL 1ra at different administration on day 2～4 of pregnancy.The level of integrin β 3 protein was measured with Westernblot method and mRNA measured with RT PCR.Results:The levels of integrin β 3 protein and mRNA were increased by LIF and IL 1(P<0.05) and which relied on administration.The level of integrin β 3 mRNA was decreased by IL 1ra (P<0.05).Conclusion:LIF and IL 1 of cytokines can improve the expression of integrin β 3 in the peri implantation endometrium of mouse.Both of them may take part in implantation by regulating cell adhesion molecules.
文摘【目的】研究NT3对早期卵泡存活、发育、激素合成的影响及其可能的作用机制,同时探讨NT3和LIF在早期卵泡启动发育方面是否存在协同或叠加效应。【方法】分离新生4d大鼠卵巢随机分配至新鲜组、基础培养组、NT3组、LIF组和NT3+LIF组,在37℃培养箱中培养14 d,经固定、包埋、切片后进行组织形态学、PCNA免疫组化、TUNEL凋亡分析,RTPCR检测KL m RNA表达及检测培养液中激素水平。【结果】相比新鲜组和基础培养组,NT3组、LIF组、NT3+LIF组中的始基卵泡比例下降,生长卵泡比例升高,同时PCNA蛋白表达显著增加;TUNEL分析显示NT3组、LIF组、NT3+LIF组的凋亡指数均显著降低;NT3、LIF组、NT3+LIF组卵巢中KL m RNA的表达量分别是对照组的(3.38±0.43)倍、(2.02±0.20)倍、(2.07±0.21)倍。【结论】NT3在早期卵泡存活、发育中有促进作用,上调KL基因表达可能是NT3的作用机制之一。NT3和LIF单独或相加均能促进卵泡发育,但两者之间无明显协同或叠加效应。
