cis-1,3,4,6-Tetranitrooctahydroimidazo-[4,5 d]imidazole(BCHMX)is an advanced energetic compound that expected to spread worldwide in the near future.Since,no approved remote detection methods were reported in current ...cis-1,3,4,6-Tetranitrooctahydroimidazo-[4,5 d]imidazole(BCHMX)is an advanced energetic compound that expected to spread worldwide in the near future.Since,no approved remote detection methods were reported in current literature for this material,we performed hyper-spectral imaging and laser induced fluorescence(LIF)to a BCHMX sample under low laser fluence for determining the optimum laser wavelength used in any future BCHMX-LIF based remote detection systems.For this purpose,an experimental setup consisted of a sun spectrum lamp and hyper-spectral camera was built to illuminate and image white powder samples of BCHMX in comparison with the traditional explosives,HMX(1,3,5,7-tetranitro-1,3,5,7-tetraazacyclooctane),RDX(1,3,5-trinitro-1,3,5-triazacyclohexane),PETN(2,2-Bis[(nitroxy)methyl]propane-1,3-diyldinitrate).The imaging reveals strong BCHMX sample absorption contrast among other samples at wavelength ranging from 400 to 410 nm.When light source was replaced by a 405 nm laser diode illuminator,a strong BCHMX sample LIF at the spectral range from 425 to 700 nm was observed under low laser fluence condition of 0.1 mJ/cm^(2).Finally,we demonstrated successfully the ability of the 405 nm LIF and the hyperspectral imaging technique to detect finger print traces of BCHMX on white cellulose fabric from a distance of 15 m and a detection limit of 1 mg/cm^(2).展开更多
The binding constant( K b) between fluorescein isothiocyanate labeled bovine serum albumin(FITC BSA) and its monoclonal antibody was determined using the ligand separation mode of affinity capillary electrophoresis(AC...The binding constant( K b) between fluorescein isothiocyanate labeled bovine serum albumin(FITC BSA) and its monoclonal antibody was determined using the ligand separation mode of affinity capillary electrophoresis(ACE) with laser induced fluorescence detection(LIF). Free FITC BSA and the complexes were separated by capillary electrophoresis in 7 min after incubation. The running buffer was 100 mmol/L HEPES at pH 7 4, and separation voltage was 30 kV. The K b was determined to be 4 7×10 8 L/mol using Scatchard plotting method. The influences of incubation time and electrophoresis conditions on determination were also studied.展开更多
文摘cis-1,3,4,6-Tetranitrooctahydroimidazo-[4,5 d]imidazole(BCHMX)is an advanced energetic compound that expected to spread worldwide in the near future.Since,no approved remote detection methods were reported in current literature for this material,we performed hyper-spectral imaging and laser induced fluorescence(LIF)to a BCHMX sample under low laser fluence for determining the optimum laser wavelength used in any future BCHMX-LIF based remote detection systems.For this purpose,an experimental setup consisted of a sun spectrum lamp and hyper-spectral camera was built to illuminate and image white powder samples of BCHMX in comparison with the traditional explosives,HMX(1,3,5,7-tetranitro-1,3,5,7-tetraazacyclooctane),RDX(1,3,5-trinitro-1,3,5-triazacyclohexane),PETN(2,2-Bis[(nitroxy)methyl]propane-1,3-diyldinitrate).The imaging reveals strong BCHMX sample absorption contrast among other samples at wavelength ranging from 400 to 410 nm.When light source was replaced by a 405 nm laser diode illuminator,a strong BCHMX sample LIF at the spectral range from 425 to 700 nm was observed under low laser fluence condition of 0.1 mJ/cm^(2).Finally,we demonstrated successfully the ability of the 405 nm LIF and the hyperspectral imaging technique to detect finger print traces of BCHMX on white cellulose fabric from a distance of 15 m and a detection limit of 1 mg/cm^(2).
文摘The binding constant( K b) between fluorescein isothiocyanate labeled bovine serum albumin(FITC BSA) and its monoclonal antibody was determined using the ligand separation mode of affinity capillary electrophoresis(ACE) with laser induced fluorescence detection(LIF). Free FITC BSA and the complexes were separated by capillary electrophoresis in 7 min after incubation. The running buffer was 100 mmol/L HEPES at pH 7 4, and separation voltage was 30 kV. The K b was determined to be 4 7×10 8 L/mol using Scatchard plotting method. The influences of incubation time and electrophoresis conditions on determination were also studied.
