为了满足乳制品中食源性致病菌精确、高效和高通量检测需求,文章以典型食源性致病菌大肠埃希氏菌O157、金黄色葡萄球菌、单核细胞增生李斯特氏菌和沙门氏菌作为目的菌,建立1种灵敏稳定的多重芯片式数字PCR(Multiplex digital chip PCR)...为了满足乳制品中食源性致病菌精确、高效和高通量检测需求,文章以典型食源性致病菌大肠埃希氏菌O157、金黄色葡萄球菌、单核细胞增生李斯特氏菌和沙门氏菌作为目的菌,建立1种灵敏稳定的多重芯片式数字PCR(Multiplex digital chip PCR)反应体系。针对4种致病菌的靶向基因,设计特异性引物,优化多重反应引物组合和反应条件,并验证其特异性和灵敏度。结果表明,该四重反应体系具有良好的特异性且未出现交叉反应。体系对4种菌株最低检出限分别达0.38、1.03、0.69 copies/μL和0.24 copies/μL,与单重反应体系检测灵敏度相当,未发生混合引物抑制反应。方法对模拟阳性样品、生乳和市售乳制品检测结果证明了其在乳原料及乳制品质量安全控制中的应用前景,文章构建的四重反应体系可为乳制品的检测与监管提供技术支持。展开更多
The paper proposes an experimental method of material inspection,which is based on digital processing of multi-frequency eddy current measurement data.The influences of various factors(conductivity,the gap between the...The paper proposes an experimental method of material inspection,which is based on digital processing of multi-frequency eddy current measurement data.The influences of various factors(conductivity,the gap between the sample surface and the sensor,the thickness of the sample) on the obtained hodographs are examined by taking the aluminum alloys for example,and the possibility of separation of various factors is analyzed.The results obtained are indicative of how much promise the proposed method offers for the inspection and testing of products made of aluminum alloys.展开更多
基金supported by Program for Basic Scientific Research of the State Academies of Sciences for 2013e2020the RF Ministry of Education and Science (Contract No. 02.G25.31.0063)
文摘The paper proposes an experimental method of material inspection,which is based on digital processing of multi-frequency eddy current measurement data.The influences of various factors(conductivity,the gap between the sample surface and the sensor,the thickness of the sample) on the obtained hodographs are examined by taking the aluminum alloys for example,and the possibility of separation of various factors is analyzed.The results obtained are indicative of how much promise the proposed method offers for the inspection and testing of products made of aluminum alloys.
