The enhancement of chalcopyrite bioleaching with an enriched microbial community by acidified seawater was studied,and the enhancing mechanism was analyzed.The microbial community was enriched at the Dabaoshan mine si...The enhancement of chalcopyrite bioleaching with an enriched microbial community by acidified seawater was studied,and the enhancing mechanism was analyzed.The microbial community was enriched at the Dabaoshan mine site,and the treated ore sample had high concentrations of chalcopyrite and galena.The experimental results show that copper extraction from chalcopyrite with an enriched microbial community in seawater was promoted from 13.1%to 62.1%by acidification in comparison with that without acidification.Further analyses of the solutions,solid residues and microbial compositions by scanning electron microscopy,X-ray diffraction,Raman spectroscopy,Fourier transform infrared spectroscopy and 16 S rDNA sequencing revealed the promoting effects of acidified seawater.This acidification can increase the biodissolution of chalcopyrite to increase the concentration of iron ions and maintain the redox potential in the range of 360−410 mV.The latter produces an optimal redox environment conducive to chalcopyrite dissolution via Cu_(2)S.The adaptability of the microbial community to a high-salt environment is improved.Chloride ions at 580 mmol/L improve the leaching kinetics of chalcopyrite by increasing the porosity and noncrystallinity of the intermediate elemental sulfur.This study provides a promising way to bioleaching copper minerals using seawater for areas with freshwater shortages.展开更多
Motivated by the business model called“community group buying”(CGB),which has emerged in China and some countries in Southeast Asia,such as Singapore and Indonesia,we develop algorithms that could help CGB platforms...Motivated by the business model called“community group buying”(CGB),which has emerged in China and some countries in Southeast Asia,such as Singapore and Indonesia,we develop algorithms that could help CGB platforms match consumers with stage-stations(the picking up center under the CGB mode).By altering the fundamental design of the existing hierarchy algorithms,improvements are achieved.It is proven that our method has a faster running speed and greater space efficiency.Our algorithms avoid traversal and compress the time complexities of matching a consumer with a stage-station and updating the storage information to O(logM)and O(MlogG),where M is the number of stage-stations and G is that of the platform’s stock-keeping units.Simulation comparisons of our algorithms with the current methods of CGB platforms show that our approaches can effectively reduce delivery costs.An interesting observation of the simula-tions is worthy of note:Increasing G may incur higher costs since it makes inventories more dispersed and delivery prob-lems more complicated.展开更多
采用累积频率的统计方法和Community Climate Model 3(CCM3)模拟的10年逐日降水结果,分析了模拟的夏季极端降水事件的时空分布特征.结果表明,CCM3模拟的极端降水阈值的大值区主要在我国黄河和长江流域的上游、印度半岛及其邻近海域和孟...采用累积频率的统计方法和Community Climate Model 3(CCM3)模拟的10年逐日降水结果,分析了模拟的夏季极端降水事件的时空分布特征.结果表明,CCM3模拟的极端降水阈值的大值区主要在我国黄河和长江流域的上游、印度半岛及其邻近海域和孟加拉湾及其北部地区.CCM3能够模拟出我国长江流域极端降水量与极端降水日数显著增加的趋势.对极端降水平均强度、降水日数以及极端降水量与总降水量比值的经验正交函数(EOF)分析可知,我国大部分地区的极端降水基本呈现同相变化,且以长江和黄河中游地区较为显著.CCM3模式基本能够模拟出观测到的极端降水阈值与总降水、极端降水日数及其距平的高空间相关性.展开更多
【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact ...【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact of this soaking method on the fungal and bacterial community structures using next-generation sequencing technology(NGS).【Methods】Lily bulbs were soaked in a seed treating agent containing beneficial microbes(SP treatment)for 4 hours.Subsequently,they were planted in soil in July and sampled in September to assess plant growth,rhizosphere soil physicochemical properties,and microorganism community structures.In addition,we employed the software PICRUSt and FUNGuild to predict bacterial pathways and fungal functions.【Results】Under SP treatment,there were significant alterations in fungi and bacteria community structures,accompanied by improved soil nutrient status.Notably,the relative abundance of dominant microorganism groups,such as the fungi Basidiomycota,Pseudeurotium,Cladophialophora,Microascus,and Dactylonectria,as well as the bacteria Proteobacteria,Chloroflexi,Ochrobactrium,Lysobacter,and RB41,underwent notable changes.Microorganism function prediction results indicated a reduction in pathotrophic fungi(including plant pathogens)and an increase in endophytic and saprotrophic fungi under SP treatment.Among the top 20 metabolism pathways,80%were upregulated in SP treatment compared to the CK.【Conclusions】Seed soaking with beneficial microbial strain promotes the growth of Lanzhou lily bulbs.The beneficial microorganisms play a crucial role in regulating soil microbial structures,enhancing the accumulation of endophytic fungi,reducing the abundance of pathogens,and improving soil functions.Furthermore,specific microbial groups are found to be involved in maintaining soil health.展开更多
[Objective]The channel straightening project of the Pinglu Canal has fragmented the river course,compromising the integrity of original river course and causing ecosystem patchiness.Understanding the current status of...[Objective]The channel straightening project of the Pinglu Canal has fragmented the river course,compromising the integrity of original river course and causing ecosystem patchiness.Understanding the current status of fish resources and the characteristics of their diversity is crucial for the ecological management of the Pinglu Canal.[Methods]During the spring and autumn in 2021 and 2022,a survey of fish resources and species diversity in the Pinglu Canal was conducted using multi-mesh gill nets.A total of 125 fish species were collected,belonging to 10 orders,34 families,and 89 genera.[Results]The result showed that the Pinglu Canal contained three nationally protected Class II species,two endemic species of the Qinjiang River,three anadromous/migratory species,and eight invasive species,accounting for 2.4%,1.6%,2.4%,and 6.4%of the total species,respectively.The fish community primarily consisted of mid-and bottom-dwelling,adhesive-egg-laying,and omnivorous species.The Shannon-Wiener,Simpson,Margalef,and Pielou indices of the fish community in the Pinglu Canal ranged from 2.347 to 2.757,0.081 to 0.151,3.493 to 4.382,and 0.812 to 0.892,respectively.These indices showed relatively uniform distribution across different river reaches.[Conclusion]The result indicate that the fish community structure in the Pinglu Canal is relatively uniform.The reach from the Yujiang River to the Shaping River shows higher stability,while other river reaches experience moderate or severe disturbances.This study provides supplementary baseline data on the fish community structure in the Pinglu Canal and explores the potential impact of inter-basin connectivity on fish resources,aiming to provide a scientific basis for habitat restoration assessments after the channel straightening project.展开更多
This study investigated the physicochemical properties,enzyme activities,volatile flavor components,microbial communities,and sensory evaluation of high-temperature Daqu(HTD)during the maturation process,and a standar...This study investigated the physicochemical properties,enzyme activities,volatile flavor components,microbial communities,and sensory evaluation of high-temperature Daqu(HTD)during the maturation process,and a standard system was established for comprehensive quality evaluation of HTD.There were obvious changes in the physicochemical properties,enzyme activities,and volatile flavor components at different storage periods,which affected the sensory evaluation of HTD to a certain extent.The results of high-throughput sequencing revealed significant microbial diversity,and showed that the bacterial community changed significantly more than did the fungal community.During the storage process,the dominant bacterial genera were Kroppenstedtia and Thermoascus.The correlation between dominant microorganisms and quality indicators highlighted their role in HTD quality.Lactococcus,Candida,Pichia,Paecilomyces,and protease activity played a crucial role in the formation of isovaleraldehyde.Acidic protease activity had the greatest impact on the microbial community.Moisture promoted isobutyric acid generation.Furthermore,the comprehensive quality evaluation standard system was established by the entropy weight method combined with multi-factor fuzzy mathematics.Consequently,this study provides innovative insights for comprehensive quality evaluation of HTD during storage and establishes a groundwork for scientific and rational storage of HTD and quality control of sauce-flavor Baijiu.展开更多
Applying bio-oxidation waste solution(BOS)to chemical-biological two-stage oxidation process can significantly improve the bio-oxidation efficiency of arsenopyrite.This study aims to clarify the enhanced oxidation mec...Applying bio-oxidation waste solution(BOS)to chemical-biological two-stage oxidation process can significantly improve the bio-oxidation efficiency of arsenopyrite.This study aims to clarify the enhanced oxidation mechanism of arsenopyrite by evaluating the effects of physical and chemical changes of arsenopyrite in BOS chemical oxidation stage on mineral dissolution kinetics,as well as microbial growth activity and community structure composition in bio-oxidation stage.The results showed that the chemical oxidation contributed to destroying the physical and chemical structure of arsenopyrite surface and reducing the particle size,and led to the formation of nitrogenous substances on mineral surface.These chemical oxidation behaviors effectively promoted Fe^(3+)cycling in the bio-oxidation system and weakened the inhibitory effect of the sulfur film on ionic diffusion,thereby enhancing the dissolution kinetics of the arsenopyrite.Therefore,the bio-oxidation efficiency of arsenopyrite was significantly increased in the two-stage oxidation process.After 18 d,the two-stage oxidation process achieved total extraction rates of(88.8±2.0)%,(86.7±1.3)%,and(74.7±3.0)%for As,Fe,and S elements,respectively.These values represented a significant increase of(50.8±3.4)%,(47.1±2.7)%,and(46.0±0.7)%,respectively,compared to the one-stage bio-oxidation process.展开更多
A new genetic algorithm for community detection in complex networks was proposed. It adopts matrix encoding that enables traditional crossover between individuals. Initial populations are generated using nodes similar...A new genetic algorithm for community detection in complex networks was proposed. It adopts matrix encoding that enables traditional crossover between individuals. Initial populations are generated using nodes similarity, which enhances the diversity of initial individuals while retaining an acceptable level of accuracy, and improves the efficiency of optimal solution search. Individual crossover is based on the quality of individuals' genes; all nodes unassigned to any community are grouped into a new community, while ambiguously placed nodes are assigned to the community to which most of their neighbors belong. Individual mutation, which splits a gene into two new genes or randomly fuses it into other genes, is non-uniform. The simplicity and effectiveness of the algorithm are revealed in experimental tests using artificial random networks and real networks. The accuracy of the algorithm is superior to that of some classic algorithms, and is comparable to that of some recent high-precision algorithms.展开更多
Simulated heap bioleaching of low-grade high pyrite-bearing chalcocite ore was conducted at 40 °C with aeration of CO_2 and N_2.Ore samples were collected at day 43,64,85,106 and subjected to microbial community ...Simulated heap bioleaching of low-grade high pyrite-bearing chalcocite ore was conducted at 40 °C with aeration of CO_2 and N_2.Ore samples were collected at day 43,64,85,106 and subjected to microbial community analysis by 16S rRNA gene clone library.Phylogenetic analyses of 16S rDNA fragments revealed that the retrieved sequences are mainly related to genus Acidithiobacillus,Leptospirillum and Sulfobacillus.Aeration of CO_2 and N_2 significantly impacted the microbial community composition.When CO_2 was aerated,the proportion of genus Acidithiobacillus considerably increased,whereas the proportion of genus Leptospirillum and genus Sulfobacillus declined.However,with the aeration of N_2,the proportion of genus Acidithiobacillus and Leptospirillum increased,but genus Sulfobacillus decreased.When there was no aeration,the microbial community was similar to the inocula with the proportion of genus Leptospirillum mounted.These results indicated that the limitation of oxygen could change the bioleaching microbial community and the aeration of CO_2 and N_2 was favourable for the growth of sulfur-oxidizer(At.caldus) and iron-oxidizer(L.ferriphilum) respectively,which could be used for the regulation of microorganisms' role in mineral bioleaching.展开更多
To reveal the impact of mining on bacterial ecology around mining area,bacterial community and geochemical characteristics about Dabaoshan Mine(Guangdong Province,China)were studied.By amplified ribosomal DNA restrict...To reveal the impact of mining on bacterial ecology around mining area,bacterial community and geochemical characteristics about Dabaoshan Mine(Guangdong Province,China)were studied.By amplified ribosomal DNA restriction analysis and phylogenetic analysis,it is found that mining pollution greatly impacts the bacterial ecology and makes the habitat type of polluted environments close to acid mine drainage(AMD)ecology.The polluted environment is acidified so greatly that neutrophil and alkaliphilic microbes are massively dead and decomposed.It provided organic matters that can make Acidiphilium sp.rapidly grow and become the most bacterial species in this niche.Furthermore,Acidithiobacillus ferrooxidans and Leptospirillum sp.are also present in this niche.The amount of Leptospirillum sp.is far more than that of Acidithiobacillus ferrooxidans,which indicates that the concentration of toxic ions is very high.The conclusions of biogeochemical analysis and microbiological monitor are identical. Moreover,because the growth of Acidithiobacillus ferrooxidans and Leptospirillum sp.depends on ferrous iron or inorganic redox sulfur compounds which can be supplied by continual AMD,their presence indicates that AMD still flows into the site.And the area is closer to the outfalls of AMD,their biomasses would be more.So the distinction of their biomasses among different areas can help us to find the effluent route of AMD.展开更多
Microbial community diversities in the drainage from three mines(Dexing Copper Mine,Qibaoshan Copper Mine and Yaogangxian Tungsten Mine,China)were analyzed using 16S rDNA PCR-RFLP approach.The efficiencies of chalcopy...Microbial community diversities in the drainage from three mines(Dexing Copper Mine,Qibaoshan Copper Mine and Yaogangxian Tungsten Mine,China)were analyzed using 16S rDNA PCR-RFLP approach.The efficiencies of chalcopyrite bioleaching were compared using enrichment of the three cultures.Phylogenetic analysis indicates that the dominant microorganisms are clustered with the Proteobacteria,the remaining is affiliated with Nitrospira,Acidobacteria and Actinobacteria.At the genus level,Acidithiobacillus is the dominant group in both YTW and QBS samples,while Spingomonas is dominant in YGX sample.Moreover,the principal component analysis(PCA)reveals that QBS and YTW have similar geochemical character and microbial communities.The results also show that pH value and tungsten concentration play a key role in microbial community distribution and relative abundance.The bioleaching efficiency of the enrichment cultures from YTW and QBS is similar.After 15 d,the bioleaching rates of low grade chalcopyrite(0.99%)are both up to 99.5% when using 10 g/L pulp density due to the similar microbial composition of YTW and QBS.Moreover,the leaching efficiencies of enrichment cultures containing multiple bioleaching microorganisms are higher than that of pure culture Acidithiobacillus ferrooxidans.展开更多
In the research on the smart community pension service,it is found that the problems encountered in the promotion of the smart pension can not be solved in the short term because of the lack of information and the acc...In the research on the smart community pension service,it is found that the problems encountered in the promotion of the smart pension can not be solved in the short term because of the lack of information and the acceptance of the intelligent products by the elderly. This paper puts forward the research ideas based on the cognitive characteristics and demand prediction of the specific population. Focusing on the research on the pension needs of people born in the 1960 s or 1970 s,it provides support for the solution of smart pension services in the peak period of aging population in the future.展开更多
Objective In 2006,Alzheimer's Association Victoria Australia funded the authors to conduct a systematic review of the community education literature in order to assist the development of their own community educat...Objective In 2006,Alzheimer's Association Victoria Australia funded the authors to conduct a systematic review of the community education literature in order to assist the development of their own community education strategy.Methods This paper draws upon this research project to convey the predominant messages arising consistently across the evidence base relating to key national health priority areas.Particular attention is given to exploring the needs of minority groups.Results The evidence reviewed indicates the need for multi-faceted intervention approaches that are carefully planned and assessed to ensure sufficient exposure of the target audience to the health messages.Conclusion Such approaches remain to be empirically tested within the context of dementia risk reduction.Nevertheless,there are consistent models in the evidence base that can be applied with confidence to community education in the current domain.展开更多
The energy metabolism structure of microbial community plays an important role in the process of biohydrometallurgy.In this article,an artificial microbial community composed of three strains(Acidithiobacillus ferroox...The energy metabolism structure of microbial community plays an important role in the process of biohydrometallurgy.In this article,an artificial microbial community composed of three strains(Acidithiobacillus ferrooxidans,Leptospirillum ferriphilum and Acidithiobacillus thiooxidans)was used to leach three kinds of chalcopyrites with different iron-sulfur ratios.After 36 d of leaching,the chalcopyrite with iron-sulfur ratio of about 1:1 achieved the highest copper extraction(69.62%).In the early stage,iron oxidizing bacteria predominated,and the expression of rus and rio was 8 times higher than that in the late stage.In the late stage,sulfur oxidizing bacteria predominated,and the expression of tetH and HdrAB was 4 times higher than that in the early stage.Furthermore,the three bioleaching systems above were added with elemental sulfur(3 g/L);the chalcopyrite with iron-sulfur ratio of about 2:1 achieved the highest copper extraction(80.63%).The results suggest that the energy metabolism structure of the microbial community could be changed by changing the iron-sulfur ratio during the leaching process for improving the leaching efficiency of chalcopyrite.展开更多
Overlapping community detection in a network is a challenging issue which attracts lots of attention in recent years.A notion of hesitant node（HN） is proposed. An HN contacts with multiple communities while the comm...Overlapping community detection in a network is a challenging issue which attracts lots of attention in recent years.A notion of hesitant node（HN） is proposed. An HN contacts with multiple communities while the communications are not strong or even accidental, thus the HN holds an implicit community structure.However, HNs are not rare in the real world network. It is important to identify them because they can be efficient hubs which form the overlapping portions of communities or simple attached nodes to some communities. Current approaches have difficulties in identifying and clustering HNs. A density-based rough set model（DBRSM） is proposed by combining the virtue of densitybased algorithms and rough set models. It incorporates the macro perspective of the community structure of the whole network and the micro perspective of the local information held by HNs, which would facilitate the further ＂growth＂ of HNs in community. We offer a theoretical support for this model from the point of strength of the trust path. The experiments on the real-world and synthetic datasets show the practical significance of analyzing and clustering the HNs based on DBRSM. Besides, the clustering based on DBRSM promotes the modularity optimization.展开更多
An minimum description length(MDL) criterion is proposed to choose a good partition for a bipartite network. A heuristic algorithm based on combination theory is presented to approach the optimal partition. As the heu...An minimum description length(MDL) criterion is proposed to choose a good partition for a bipartite network. A heuristic algorithm based on combination theory is presented to approach the optimal partition. As the heuristic algorithm automatically searches for the number of partitions, no user intervention is required. Finally, experiments are conducted on various datasets, and the results show that our method generates higher quality results than the state-of-art methods, cross-association and bipartite, recursively induced modules. Experiment results also show the good scalability of the proposed algorithm. The method is applied to traditional Chinese medicine(TCM) formula and Chinese herbal network whose community structure is not well known, and found that it detects significant and it is informative community division.展开更多
The human society is an interdependent community of shared future.Rooted in the foundation of “Great Harmony” ideology in Chinese traditional culture,the idea of “a community of shared future for mankind” is an im...The human society is an interdependent community of shared future.Rooted in the foundation of “Great Harmony” ideology in Chinese traditional culture,the idea of “a community of shared future for mankind” is an important part of the socialism thoughts with Chinese characteristics in new era.The similarities and differences of cultures are the base of complementation and premise of innovative development rather than the source of conflicts.The core value of community of shared future is harmony,which with the goal of win-win can be achieved only by actively advocating cultural diversity,strengthening the effective and equal dialogue and understanding and complementing between different cultures or civilizations,and creating an inclusive,tolerant and reasonable peaceful environment.In this respect,the comparative literature research should inevitably play its irreplaceable role as a bridge to enable the communication,dialogue and understanding between eastern and western literature and cultures.The communication between cultures is homogeneous but the interaction between cultural soils as well as national cultures is heterogeneous.During the communication process of literature works,it’s necessary to further study the literature content under cultural differences.The community of shared future for mankind is not only a new idea for global governance but also a new kind of expression of the habitat guarding and building awareness;it is the real and physically objective existence and also the imaginary and emotional spirit pursuit.In the context that the comparative literature is trying to develop into the world literature,it’s the mission for mankind to make it possible the wide transmission,reading and studying of the diversified and unique literature works of different countries,which can manifest the common value connotation of human beings,and to spare no effort to remove obstacles from the path to the destination of a community of shared future for mankind.展开更多
This paper describes the experience of introducing Environmental education in small rural local communities, streets in neighborhood towns in African countries.It further explains the role of indigenous community in m...This paper describes the experience of introducing Environmental education in small rural local communities, streets in neighborhood towns in African countries.It further explains the role of indigenous community in management of natural resources, environmental conservation and the need of decentralized governance aiming at providing authorities from the grass root level.The research has been carefully studied from the experience of African countries;some developed countries were several researches have been undertaken.Since the indigenous communities have little knowledge of understanding better their environment,its展开更多
基金Project(2022YFC2105300)supported by the National Key Research and Development Program of ChinaProjects(41802038,51774342)supported by the National Natural Science Foundation of China。
文摘The enhancement of chalcopyrite bioleaching with an enriched microbial community by acidified seawater was studied,and the enhancing mechanism was analyzed.The microbial community was enriched at the Dabaoshan mine site,and the treated ore sample had high concentrations of chalcopyrite and galena.The experimental results show that copper extraction from chalcopyrite with an enriched microbial community in seawater was promoted from 13.1%to 62.1%by acidification in comparison with that without acidification.Further analyses of the solutions,solid residues and microbial compositions by scanning electron microscopy,X-ray diffraction,Raman spectroscopy,Fourier transform infrared spectroscopy and 16 S rDNA sequencing revealed the promoting effects of acidified seawater.This acidification can increase the biodissolution of chalcopyrite to increase the concentration of iron ions and maintain the redox potential in the range of 360−410 mV.The latter produces an optimal redox environment conducive to chalcopyrite dissolution via Cu_(2)S.The adaptability of the microbial community to a high-salt environment is improved.Chloride ions at 580 mmol/L improve the leaching kinetics of chalcopyrite by increasing the porosity and noncrystallinity of the intermediate elemental sulfur.This study provides a promising way to bioleaching copper minerals using seawater for areas with freshwater shortages.
基金supported by the National Natural Science Foundation of China(71991464,71921001)Fundamental Research Funds for the Central Universities,General Program(WK2040000053)Key Program(YD2040002027)。
文摘Motivated by the business model called“community group buying”(CGB),which has emerged in China and some countries in Southeast Asia,such as Singapore and Indonesia,we develop algorithms that could help CGB platforms match consumers with stage-stations(the picking up center under the CGB mode).By altering the fundamental design of the existing hierarchy algorithms,improvements are achieved.It is proven that our method has a faster running speed and greater space efficiency.Our algorithms avoid traversal and compress the time complexities of matching a consumer with a stage-station and updating the storage information to O(logM)and O(MlogG),where M is the number of stage-stations and G is that of the platform’s stock-keeping units.Simulation comparisons of our algorithms with the current methods of CGB platforms show that our approaches can effectively reduce delivery costs.An interesting observation of the simula-tions is worthy of note:Increasing G may incur higher costs since it makes inventories more dispersed and delivery prob-lems more complicated.
文摘采用累积频率的统计方法和Community Climate Model 3(CCM3)模拟的10年逐日降水结果,分析了模拟的夏季极端降水事件的时空分布特征.结果表明,CCM3模拟的极端降水阈值的大值区主要在我国黄河和长江流域的上游、印度半岛及其邻近海域和孟加拉湾及其北部地区.CCM3能够模拟出我国长江流域极端降水量与极端降水日数显著增加的趋势.对极端降水平均强度、降水日数以及极端降水量与总降水量比值的经验正交函数(EOF)分析可知,我国大部分地区的极端降水基本呈现同相变化,且以长江和黄河中游地区较为显著.CCM3模式基本能够模拟出观测到的极端降水阈值与总降水、极端降水日数及其距平的高空间相关性.
文摘【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact of this soaking method on the fungal and bacterial community structures using next-generation sequencing technology(NGS).【Methods】Lily bulbs were soaked in a seed treating agent containing beneficial microbes(SP treatment)for 4 hours.Subsequently,they were planted in soil in July and sampled in September to assess plant growth,rhizosphere soil physicochemical properties,and microorganism community structures.In addition,we employed the software PICRUSt and FUNGuild to predict bacterial pathways and fungal functions.【Results】Under SP treatment,there were significant alterations in fungi and bacteria community structures,accompanied by improved soil nutrient status.Notably,the relative abundance of dominant microorganism groups,such as the fungi Basidiomycota,Pseudeurotium,Cladophialophora,Microascus,and Dactylonectria,as well as the bacteria Proteobacteria,Chloroflexi,Ochrobactrium,Lysobacter,and RB41,underwent notable changes.Microorganism function prediction results indicated a reduction in pathotrophic fungi(including plant pathogens)and an increase in endophytic and saprotrophic fungi under SP treatment.Among the top 20 metabolism pathways,80%were upregulated in SP treatment compared to the CK.【Conclusions】Seed soaking with beneficial microbial strain promotes the growth of Lanzhou lily bulbs.The beneficial microorganisms play a crucial role in regulating soil microbial structures,enhancing the accumulation of endophytic fungi,reducing the abundance of pathogens,and improving soil functions.Furthermore,specific microbial groups are found to be involved in maintaining soil health.
文摘[Objective]The channel straightening project of the Pinglu Canal has fragmented the river course,compromising the integrity of original river course and causing ecosystem patchiness.Understanding the current status of fish resources and the characteristics of their diversity is crucial for the ecological management of the Pinglu Canal.[Methods]During the spring and autumn in 2021 and 2022,a survey of fish resources and species diversity in the Pinglu Canal was conducted using multi-mesh gill nets.A total of 125 fish species were collected,belonging to 10 orders,34 families,and 89 genera.[Results]The result showed that the Pinglu Canal contained three nationally protected Class II species,two endemic species of the Qinjiang River,three anadromous/migratory species,and eight invasive species,accounting for 2.4%,1.6%,2.4%,and 6.4%of the total species,respectively.The fish community primarily consisted of mid-and bottom-dwelling,adhesive-egg-laying,and omnivorous species.The Shannon-Wiener,Simpson,Margalef,and Pielou indices of the fish community in the Pinglu Canal ranged from 2.347 to 2.757,0.081 to 0.151,3.493 to 4.382,and 0.812 to 0.892,respectively.These indices showed relatively uniform distribution across different river reaches.[Conclusion]The result indicate that the fish community structure in the Pinglu Canal is relatively uniform.The reach from the Yujiang River to the Shaping River shows higher stability,while other river reaches experience moderate or severe disturbances.This study provides supplementary baseline data on the fish community structure in the Pinglu Canal and explores the potential impact of inter-basin connectivity on fish resources,aiming to provide a scientific basis for habitat restoration assessments after the channel straightening project.
文摘This study investigated the physicochemical properties,enzyme activities,volatile flavor components,microbial communities,and sensory evaluation of high-temperature Daqu(HTD)during the maturation process,and a standard system was established for comprehensive quality evaluation of HTD.There were obvious changes in the physicochemical properties,enzyme activities,and volatile flavor components at different storage periods,which affected the sensory evaluation of HTD to a certain extent.The results of high-throughput sequencing revealed significant microbial diversity,and showed that the bacterial community changed significantly more than did the fungal community.During the storage process,the dominant bacterial genera were Kroppenstedtia and Thermoascus.The correlation between dominant microorganisms and quality indicators highlighted their role in HTD quality.Lactococcus,Candida,Pichia,Paecilomyces,and protease activity played a crucial role in the formation of isovaleraldehyde.Acidic protease activity had the greatest impact on the microbial community.Moisture promoted isobutyric acid generation.Furthermore,the comprehensive quality evaluation standard system was established by the entropy weight method combined with multi-factor fuzzy mathematics.Consequently,this study provides innovative insights for comprehensive quality evaluation of HTD during storage and establishes a groundwork for scientific and rational storage of HTD and quality control of sauce-flavor Baijiu.
基金Project(52274348)supported by the National Natural Science Foundation of ChinaProject(2022JH1/10400024)supported by the Major Projects for the“Revealed Top”Science and Technology of Liaoning Province,China。
文摘Applying bio-oxidation waste solution(BOS)to chemical-biological two-stage oxidation process can significantly improve the bio-oxidation efficiency of arsenopyrite.This study aims to clarify the enhanced oxidation mechanism of arsenopyrite by evaluating the effects of physical and chemical changes of arsenopyrite in BOS chemical oxidation stage on mineral dissolution kinetics,as well as microbial growth activity and community structure composition in bio-oxidation stage.The results showed that the chemical oxidation contributed to destroying the physical and chemical structure of arsenopyrite surface and reducing the particle size,and led to the formation of nitrogenous substances on mineral surface.These chemical oxidation behaviors effectively promoted Fe^(3+)cycling in the bio-oxidation system and weakened the inhibitory effect of the sulfur film on ionic diffusion,thereby enhancing the dissolution kinetics of the arsenopyrite.Therefore,the bio-oxidation efficiency of arsenopyrite was significantly increased in the two-stage oxidation process.After 18 d,the two-stage oxidation process achieved total extraction rates of(88.8±2.0)%,(86.7±1.3)%,and(74.7±3.0)%for As,Fe,and S elements,respectively.These values represented a significant increase of(50.8±3.4)%,(47.1±2.7)%,and(46.0±0.7)%,respectively,compared to the one-stage bio-oxidation process.
文摘A new genetic algorithm for community detection in complex networks was proposed. It adopts matrix encoding that enables traditional crossover between individuals. Initial populations are generated using nodes similarity, which enhances the diversity of initial individuals while retaining an acceptable level of accuracy, and improves the efficiency of optimal solution search. Individual crossover is based on the quality of individuals' genes; all nodes unassigned to any community are grouped into a new community, while ambiguously placed nodes are assigned to the community to which most of their neighbors belong. Individual mutation, which splits a gene into two new genes or randomly fuses it into other genes, is non-uniform. The simplicity and effectiveness of the algorithm are revealed in experimental tests using artificial random networks and real networks. The accuracy of the algorithm is superior to that of some classic algorithms, and is comparable to that of some recent high-precision algorithms.
基金Project(51404033)supported by the National Natural Science Foundation of ChinaProject(2010CB630905)supported by the National Basic Research Program of China
文摘Simulated heap bioleaching of low-grade high pyrite-bearing chalcocite ore was conducted at 40 °C with aeration of CO_2 and N_2.Ore samples were collected at day 43,64,85,106 and subjected to microbial community analysis by 16S rRNA gene clone library.Phylogenetic analyses of 16S rDNA fragments revealed that the retrieved sequences are mainly related to genus Acidithiobacillus,Leptospirillum and Sulfobacillus.Aeration of CO_2 and N_2 significantly impacted the microbial community composition.When CO_2 was aerated,the proportion of genus Acidithiobacillus considerably increased,whereas the proportion of genus Leptospirillum and genus Sulfobacillus declined.However,with the aeration of N_2,the proportion of genus Acidithiobacillus and Leptospirillum increased,but genus Sulfobacillus decreased.When there was no aeration,the microbial community was similar to the inocula with the proportion of genus Leptospirillum mounted.These results indicated that the limitation of oxygen could change the bioleaching microbial community and the aeration of CO_2 and N_2 was favourable for the growth of sulfur-oxidizer(At.caldus) and iron-oxidizer(L.ferriphilum) respectively,which could be used for the regulation of microorganisms' role in mineral bioleaching.
基金Project(50621063)supported by the Science Fund for Creative Research Groups of ChinaProject(2004CB619201)supported by the Major State Basic Research Development Program of China
文摘To reveal the impact of mining on bacterial ecology around mining area,bacterial community and geochemical characteristics about Dabaoshan Mine(Guangdong Province,China)were studied.By amplified ribosomal DNA restriction analysis and phylogenetic analysis,it is found that mining pollution greatly impacts the bacterial ecology and makes the habitat type of polluted environments close to acid mine drainage(AMD)ecology.The polluted environment is acidified so greatly that neutrophil and alkaliphilic microbes are massively dead and decomposed.It provided organic matters that can make Acidiphilium sp.rapidly grow and become the most bacterial species in this niche.Furthermore,Acidithiobacillus ferrooxidans and Leptospirillum sp.are also present in this niche.The amount of Leptospirillum sp.is far more than that of Acidithiobacillus ferrooxidans,which indicates that the concentration of toxic ions is very high.The conclusions of biogeochemical analysis and microbiological monitor are identical. Moreover,because the growth of Acidithiobacillus ferrooxidans and Leptospirillum sp.depends on ferrous iron or inorganic redox sulfur compounds which can be supplied by continual AMD,their presence indicates that AMD still flows into the site.And the area is closer to the outfalls of AMD,their biomasses would be more.So the distinction of their biomasses among different areas can help us to find the effluent route of AMD.
基金Project(50621063) supported by the National Natural Science Foundation of ChinaProject (2004CB619204) supported by the National Basic Research and Development Program of China
文摘Microbial community diversities in the drainage from three mines(Dexing Copper Mine,Qibaoshan Copper Mine and Yaogangxian Tungsten Mine,China)were analyzed using 16S rDNA PCR-RFLP approach.The efficiencies of chalcopyrite bioleaching were compared using enrichment of the three cultures.Phylogenetic analysis indicates that the dominant microorganisms are clustered with the Proteobacteria,the remaining is affiliated with Nitrospira,Acidobacteria and Actinobacteria.At the genus level,Acidithiobacillus is the dominant group in both YTW and QBS samples,while Spingomonas is dominant in YGX sample.Moreover,the principal component analysis(PCA)reveals that QBS and YTW have similar geochemical character and microbial communities.The results also show that pH value and tungsten concentration play a key role in microbial community distribution and relative abundance.The bioleaching efficiency of the enrichment cultures from YTW and QBS is similar.After 15 d,the bioleaching rates of low grade chalcopyrite(0.99%)are both up to 99.5% when using 10 g/L pulp density due to the similar microbial composition of YTW and QBS.Moreover,the leaching efficiencies of enrichment cultures containing multiple bioleaching microorganisms are higher than that of pure culture Acidithiobacillus ferrooxidans.
基金This paper is the research result of the social science planning project of Chongqing(subject number:2018QNYS70)and the educational planning project of Chongqing(subject number:2017-GX-273).
文摘In the research on the smart community pension service,it is found that the problems encountered in the promotion of the smart pension can not be solved in the short term because of the lack of information and the acceptance of the intelligent products by the elderly. This paper puts forward the research ideas based on the cognitive characteristics and demand prediction of the specific population. Focusing on the research on the pension needs of people born in the 1960 s or 1970 s,it provides support for the solution of smart pension services in the peak period of aging population in the future.
文摘Objective In 2006,Alzheimer's Association Victoria Australia funded the authors to conduct a systematic review of the community education literature in order to assist the development of their own community education strategy.Methods This paper draws upon this research project to convey the predominant messages arising consistently across the evidence base relating to key national health priority areas.Particular attention is given to exploring the needs of minority groups.Results The evidence reviewed indicates the need for multi-faceted intervention approaches that are carefully planned and assessed to ensure sufficient exposure of the target audience to the health messages.Conclusion Such approaches remain to be empirically tested within the context of dementia risk reduction.Nevertheless,there are consistent models in the evidence base that can be applied with confidence to community education in the current domain.
基金Project(2017zzts382)supported by Central South University Postgraduate Independent Exploration and Innovation,ChinaProject(2014jpkc003)supported by Central South University Graduate Excellent Course,China+1 种基金Project(2015JJ2165)supported by Hunan Provincial Natural Science Foundation of ChinaProject(165611031)supported by Central South University Fundamental Research Funds Special Funding,China。
文摘The energy metabolism structure of microbial community plays an important role in the process of biohydrometallurgy.In this article,an artificial microbial community composed of three strains(Acidithiobacillus ferrooxidans,Leptospirillum ferriphilum and Acidithiobacillus thiooxidans)was used to leach three kinds of chalcopyrites with different iron-sulfur ratios.After 36 d of leaching,the chalcopyrite with iron-sulfur ratio of about 1:1 achieved the highest copper extraction(69.62%).In the early stage,iron oxidizing bacteria predominated,and the expression of rus and rio was 8 times higher than that in the late stage.In the late stage,sulfur oxidizing bacteria predominated,and the expression of tetH and HdrAB was 4 times higher than that in the early stage.Furthermore,the three bioleaching systems above were added with elemental sulfur(3 g/L);the chalcopyrite with iron-sulfur ratio of about 2:1 achieved the highest copper extraction(80.63%).The results suggest that the energy metabolism structure of the microbial community could be changed by changing the iron-sulfur ratio during the leaching process for improving the leaching efficiency of chalcopyrite.
基金supported by the National Natural Science Foundation of China(71271018)
文摘Overlapping community detection in a network is a challenging issue which attracts lots of attention in recent years.A notion of hesitant node（HN） is proposed. An HN contacts with multiple communities while the communications are not strong or even accidental, thus the HN holds an implicit community structure.However, HNs are not rare in the real world network. It is important to identify them because they can be efficient hubs which form the overlapping portions of communities or simple attached nodes to some communities. Current approaches have difficulties in identifying and clustering HNs. A density-based rough set model（DBRSM） is proposed by combining the virtue of densitybased algorithms and rough set models. It incorporates the macro perspective of the community structure of the whole network and the micro perspective of the local information held by HNs, which would facilitate the further ＂growth＂ of HNs in community. We offer a theoretical support for this model from the point of strength of the trust path. The experiments on the real-world and synthetic datasets show the practical significance of analyzing and clustering the HNs based on DBRSM. Besides, the clustering based on DBRSM promotes the modularity optimization.
基金Projects(61363037,31071700)supported by the National Natural Science Foundation of ChinaProject(2011GXNSFD018025)supported by the Natural Science Key Foundation of Guangxi Province,ChinaProject(KYTZ201108)supported by the Development Foundation of Chengdu University of Information Technology,China
文摘An minimum description length(MDL) criterion is proposed to choose a good partition for a bipartite network. A heuristic algorithm based on combination theory is presented to approach the optimal partition. As the heuristic algorithm automatically searches for the number of partitions, no user intervention is required. Finally, experiments are conducted on various datasets, and the results show that our method generates higher quality results than the state-of-art methods, cross-association and bipartite, recursively induced modules. Experiment results also show the good scalability of the proposed algorithm. The method is applied to traditional Chinese medicine(TCM) formula and Chinese herbal network whose community structure is not well known, and found that it detects significant and it is informative community division.
基金funded by 2012 Shandong University Independent Innovation (Humanity and Social Science Special Program) Project “Study of British Immigrant Writer Caryl Philips”(IFW12018)
文摘The human society is an interdependent community of shared future.Rooted in the foundation of “Great Harmony” ideology in Chinese traditional culture,the idea of “a community of shared future for mankind” is an important part of the socialism thoughts with Chinese characteristics in new era.The similarities and differences of cultures are the base of complementation and premise of innovative development rather than the source of conflicts.The core value of community of shared future is harmony,which with the goal of win-win can be achieved only by actively advocating cultural diversity,strengthening the effective and equal dialogue and understanding and complementing between different cultures or civilizations,and creating an inclusive,tolerant and reasonable peaceful environment.In this respect,the comparative literature research should inevitably play its irreplaceable role as a bridge to enable the communication,dialogue and understanding between eastern and western literature and cultures.The communication between cultures is homogeneous but the interaction between cultural soils as well as national cultures is heterogeneous.During the communication process of literature works,it’s necessary to further study the literature content under cultural differences.The community of shared future for mankind is not only a new idea for global governance but also a new kind of expression of the habitat guarding and building awareness;it is the real and physically objective existence and also the imaginary and emotional spirit pursuit.In the context that the comparative literature is trying to develop into the world literature,it’s the mission for mankind to make it possible the wide transmission,reading and studying of the diversified and unique literature works of different countries,which can manifest the common value connotation of human beings,and to spare no effort to remove obstacles from the path to the destination of a community of shared future for mankind.
文摘This paper describes the experience of introducing Environmental education in small rural local communities, streets in neighborhood towns in African countries.It further explains the role of indigenous community in management of natural resources, environmental conservation and the need of decentralized governance aiming at providing authorities from the grass root level.The research has been carefully studied from the experience of African countries;some developed countries were several researches have been undertaken.Since the indigenous communities have little knowledge of understanding better their environment,its
