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New density clustering-based approach for failure mode and effect analysis considering opinion evolution and bounded confidence
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作者 WANG Jian ZHU Jingyi +1 位作者 SHI Hua LIU Huchen 《Journal of Systems Engineering and Electronics》 CSCD 2024年第6期1491-1506,共16页
Failure mode and effect analysis(FMEA)is a preven-tative risk evaluation method used to evaluate and eliminate fail-ure modes within a system.However,the traditional FMEA method exhibits many deficiencies that pose ch... Failure mode and effect analysis(FMEA)is a preven-tative risk evaluation method used to evaluate and eliminate fail-ure modes within a system.However,the traditional FMEA method exhibits many deficiencies that pose challenges in prac-tical applications.To improve the conventional FMEA,many modified FMEA models have been suggested.However,the majority of them inadequately address consensus issues and focus on achieving a complete ranking of failure modes.In this research,we propose a new FMEA approach that integrates a two-stage consensus reaching model and a density peak clus-tering algorithm for the assessment and clustering of failure modes.Firstly,we employ the interval 2-tuple linguistic vari-ables(I2TLVs)to express the uncertain risk evaluations provided by FMEA experts.Then,a two-stage consensus reaching model is adopted to enable FMEA experts to reach a consensus.Next,failure modes are categorized into several risk clusters using a density peak clustering algorithm.Finally,the proposed FMEA is illustrated by a case study of load-bearing guidance devices of subway systems.The results show that the proposed FMEA model can more easily to describe the uncertain risk information of failure modes by using the I2TLVs;the introduction of an endogenous feedback mechanism and an exogenous feedback mechanism can accelerate the process of consensus reaching;and the density peak clustering of failure modes successfully improves the practical applicability of FMEA. 展开更多
关键词 failure mode and effect analysis(FMEA) interval 2-tuple linguistic variable(I2TLV) consensus reaching density peak clustering algorithm
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Vertical Migrating and Cluster Analysis of Soil Mesofauna at Dongying Halophytes Garden in Yellow River Delta 被引量:3
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作者 He Fu-xia Xie Tong-yin +1 位作者 Xie Gui-lin Fu Rong-shu 《Journal of Northeast Agricultural University(English Edition)》 CAS 2014年第1期25-30,共6页
For the first time, we used Tullgren method made a study on vertical migrating and cluster analysis of the soil mesofauna in Dongying Halophytes Garden in the Yellow River Delta (YRD), Shandong Province. The results... For the first time, we used Tullgren method made a study on vertical migrating and cluster analysis of the soil mesofauna in Dongying Halophytes Garden in the Yellow River Delta (YRD), Shandong Province. The results showed that the soil mesofauna tended to gather on soil surface in most samples at most times, but the vertical migrating greatly varied in different seasons or environment conditions. Acari was the dominant group. The index of diversity of the soil fauna was correlated with the index of evenness. The Acari's number of individuals infected other species and numbers. Dominant group-Aeari made greater contribution to the result of cluster analysis, and there were significant differences between communities in different habitats by cluster analysis with both Bray-Curtis and Jaccard similarity coefficient. 展开更多
关键词 HALOPHYTES soil mesofauna vertical migrating cluster analysis
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The Fuzzy Cluster Analysis in Identification of Key Temperatures in Machine Tool
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作者 ZHAO Da-quan 1, ZHENG Li 1, XIANG Wei-hong 1, LI Kang 1, LIU Da-cheng 1, ZHANG Bo-peng 2 (1. Department of Industrial Engineering, Tsinghua University, 2. Department of Precision Instruments and Mechanology, Tsinghua University, B eijing 100084, China) 《厦门大学学报(自然科学版)》 CAS CSCD 北大核心 2002年第S1期88-89,共2页
The thermal-induced error is a very important sour ce of machining errors of machine tools. To compensate the thermal-induced machin ing errors, a relationship model between the thermal field and deformations was need... The thermal-induced error is a very important sour ce of machining errors of machine tools. To compensate the thermal-induced machin ing errors, a relationship model between the thermal field and deformations was needed. The relationship can be deduced by virtual of FEM (Finite Element Method ), ANN (Artificial Neural Network) or MRA (Multiple Regression Analysis). MR A is on the basis of a total understanding of the temperature distribution of th e machine tool. Although the more the temperatures measured are, the more accura te the MRA is, too more temperatures will hinder the analysis calculation. So it is necessary to identify the key temperatures of the machine tool. The selectio n of key temperatures decides the efficiency and precision of MRA. Because of th e complexities and multi-input and multi-output structure of the relationships , the exact quantitative portions as well as the unclear portions must be taken into consideration together to improve the identification of key temperatures. I n this paper, a fuzzy cluster analysis was used to select the key temperatures. The substance of identifying the key temperatures is to group all temperatures b y their relativity, and then to select a temperature from each group as the repr esentation. A fuzzy cluster analysis can uncover the relationships between t he thermal field and deformations more truly and thoroughly. A fuzzy cluster ana lysis is the cluster analysis based on fuzzy sets. Given U={u i|i=0,...,N}, in which u i is the temperature measured, a fuzzy matrix R can be obta ined. The transfer close package t(R) can be deduced from R. A fuzzy clu ster of U then conducts on the basis of t(R). Based on the fuzzy cluster analysis discussed above, this paper identified the k ey temperatures of a horizontal machining center. The number of the temperatures measured was reduced to 4 from 32, and then the multiple regression relationshi p models between the 4 temperatures and the thermal deformations of the spindle were drawn. The remnant errors between the regression models and measured deform ations reached a satisfying low level. At the same time, the decreasing of tempe rature variable number improved the efficiency of measure and analysis greatly. 展开更多
关键词 The Fuzzy cluster analysis in Identification of Key Temperatures in Machine Tool
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基于DBSCAN的改进RANSAC点云平面拟合算法
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作者 叶锦华 林旭敏 吴海彬 《湖南大学学报(自然科学版)》 北大核心 2025年第2期76-87,共12页
针对随机采样一致性(random sample consensus,RANSAC)算法对含有噪声的点云数据进行平面拟合时效果不佳和容易产生误识别的问题,对算法进行改进.通过基于密度的噪声应用空间聚类(density-based spatial clustering of applications wit... 针对随机采样一致性(random sample consensus,RANSAC)算法对含有噪声的点云数据进行平面拟合时效果不佳和容易产生误识别的问题,对算法进行改进.通过基于密度的噪声应用空间聚类(density-based spatial clustering of applications with noise,DBSCAN)算法改变RANSAC算法初始点集合的选择策略,并使用主成分分析法(principal component analysis,PCA)计算点云各点法向量,以点到平面距离以及点的法向量与平面法向量夹角两个约束条件同时作为RANSAC算法平面拟合模型内点判定的准则.采用无噪声与分别含有300个噪声点和500个噪声点的点云仿真数据进行测试,本文算法拟合结果均接近理论值且内点距离标准差分别为1.007×10-8、0.003、0.007,优于RANSAC算法.采用实际工件点云数据在两种工况场景下进行测试,本文算法拟合平面内点比率相对于传统RANSAC算法分别提高24.7%和24.6%,平面提取完整度及准确率同样优于RANSAC算法.仿真模拟及实例分析证明了本文算法的有效性. 展开更多
关键词 点云平面拟合 随机采样一致性 噪声 密度聚类 主成分分析
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Group decision-making method based on entropy and experts cluster analysis 被引量:12
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作者 Xuan Zhou Fengming Zhang Xiaobin Hui Kewu Li 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2011年第3期468-472,共5页
According to the aggregation method of experts' evaluation information in group decision-making,the existing methods of determining experts' weights based on cluster analysis take into account the expert's preferen... According to the aggregation method of experts' evaluation information in group decision-making,the existing methods of determining experts' weights based on cluster analysis take into account the expert's preferences and the consistency of expert's collating vectors,but they lack of the measure of information similarity.So it may occur that although the collating vector is similar to the group consensus,information uncertainty is great of a certain expert.However,it is clustered to a larger group and given a high weight.For this,a new aggregation method based on entropy and cluster analysis in group decision-making process is provided,in which the collating vectors are classified with information similarity coefficient,and the experts' weights are determined according to the result of classification,the entropy of collating vectors and the judgment matrix consistency.Finally,a numerical example shows that the method is feasible and effective. 展开更多
关键词 group decision-making judgment matrix ENTROPY information similarity coefficient cluster analysis.
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Correlation and Path Coefficient and Chi-square Distance Cluster Analysis for Several Characteristics in Tobacco Germplasm Resource 被引量:1
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作者 LI Wenping ZHU Lieshu +3 位作者 ZHAO Songyi LIANG Qizheng WANG Yuchao TAN Xi 《Journal of Northeast Agricultural University(English Edition)》 CAS 2010年第1期10-15,共6页
Correlation and path coefficient analyses were conducted for 10 characteristics of 24 pure lines of flue-cured tobacco such as plant height, knot distance, leaf number, the central leaf length and width, ratio of the ... Correlation and path coefficient analyses were conducted for 10 characteristics of 24 pure lines of flue-cured tobacco such as plant height, knot distance, leaf number, the central leaf length and width, ratio of the length to width, stem girth, dates of budding, leaf yield and ratio of the prime-medium tobacco. The leaf number and the central leaf length showed a positive or a strong positive correlation with the yield per plant. And the leaf number and leaf yield per plant showed a strong positive correlation with the ratio of prime-medium tobacco. The results showed that the leaf yield per plant among these characteristics played a major role in determining the ratio of prime-medium tobacco while the others were less related with the ratio. Square sum of deviation method cluster analyses showed that 24 pure lines of flue-cured tobacco were clustered into two groups. Of the pure lines, Line T1706 and Line T1245 had a far relationship with all other lines, and also had a heterosis when crossed with the other lines. Lines Guangdonghuang 1 and R72(3)B-2-1 were closely related. 展开更多
关键词 flue-cured tobacco correlation analysis path coefficient analysis cluster analysis
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基于改进K近邻-密度峰值聚类的多模块PCA过程监测
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作者 王竞志 徐琛 +1 位作者 陶洪峰 杨慧中 《控制工程》 北大核心 2025年第4期664-673,共10页
聚类方法考虑了变量之间的复杂关系,已被广泛应用于多模块过程监测中。但传统方法仍需要一些先验知识,如聚类个数等,限制了其应用范围。因此,提出了一种基于改进K近邻-密度峰值聚类的多模块主元分析(principal component analysis,PCA)... 聚类方法考虑了变量之间的复杂关系,已被广泛应用于多模块过程监测中。但传统方法仍需要一些先验知识,如聚类个数等,限制了其应用范围。因此,提出了一种基于改进K近邻-密度峰值聚类的多模块主元分析(principal component analysis,PCA)过程监测方法。首先,引入信息距离,基于变量之间的信息熵构造信息距离矩阵;然后,计算决策值和其下降趋势变化幅值,通过寻找极大值的方式自动确定聚类个数,即模块个数;最后,通过K近邻优化的分配策略完成模块划分,并建立基于PCA的过程监测模型,所有模块的监测结果通过贝叶斯推论进行融合。将所提方法应用在数值仿真和田纳西-伊斯曼(Tennessee-Eastman,TE)过程仿真中,仿真结果验证了所提方法的有效性。 展开更多
关键词 多模块 过程监测 信息距离 聚类 主元分析
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盘州大厂茶(Camellia tachangensis)的生物碱类、儿茶素类及氨基酸类组成特性分析
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作者 杨春 郭燕 +6 位作者 乔大河 梁思慧 李燕 李彦福 密孝增 杨世雄 陈正武 《西南农业学报》 北大核心 2025年第3期507-518,共12页
【目的】探明盘州大厂茶生化组成特性,为贵州野生茶树的系统鉴定,特色盘州大厂茶新品种选育、种质资源保护及开发利用提供依据。【方法】采用高效液相色谱法对25份盘州大厂茶及3份对照的29种生化组分进行检测,结合OPLS-DA分析、相关性... 【目的】探明盘州大厂茶生化组成特性,为贵州野生茶树的系统鉴定,特色盘州大厂茶新品种选育、种质资源保护及开发利用提供依据。【方法】采用高效液相色谱法对25份盘州大厂茶及3份对照的29种生化组分进行检测,结合OPLS-DA分析、相关性分析、主成分分析和聚类分析对盘州大厂茶生化组成特性及生化品质进行综合评价。【结果】盘州大厂茶种质存在丰富的遗传多样性,28种生化组分变异系数为0.79%(GCG)~241.4%(丙氨酸),平均为46.08%;遗传多样性指数为0.861(丙氨酸)~2.001(EC),平均为1.798。苦茶碱、ECG和茶氨酸分别构成盘州大厂茶中的优势生物碱类、儿茶素类和氨基酸类。通过OPLS-DA分析,筛选出11种VIP大于1.0的生化组分:咖啡碱、EGCG、苦茶碱、半胱氨酸、脯氨酸、GCG、甲硫氨酸、ECG、EGC、茶氨酸和EC,可用于区分盘州大厂茶和栽培种茶树。28种生化组分间的相关关系存在一定规律,生物碱类与氨基酸类的相关关系强于其与儿茶素类间的相关关系;儿茶素类间存在一定的协同性与拮抗性,EC与EGCG、GCG呈显著负相关,EGCG与EGC、ECG呈极显著正相关;氨基酸类间相关性较强,且多为正相关,脯氨酸、酪氨酸、赖氨酸、异亮氨酸、亮氨酸和苯丙氨酸相互间呈极显著正相关。主成分分析提取到8种主成分,累计贡献率为82.834%,第1主成分的决定生化因子异亮氨酸、亮氨酸、丝氨酸、赖氨酸、苯丙氨酸和脯氨酸是评价盘州大厂茶生化品质的关键。聚类分析将25份盘州大厂茶分为2类,A类包括PZ-01、PZ-02、PZ-03等8份盘州大厂茶种质,其余种质为B类,其中,A类的苦茶碱和精氨酸含量显著高于B类,丝氨酸、苏氨酸、脯氨酸、酪氨酸、赖氨酸、异亮氨酸、亮氨酸和苯丙氨酸含量极显著高于B类。【结论】盘州大厂茶具有生化特异性,苦茶碱、EC、ECG和甲硫氨酸是盘州大厂茶的特异高积累生化组分。氨基酸类既是评价盘州大厂茶种质生化品质的关键也是造成盘州大厂茶种质内部分化的关键。PZ-01、PZ-02、PZ-06、PZ-09和PZ-16具备培育特异茶树新品种的应用价值。 展开更多
关键词 盘州 大厂茶 苦茶碱 主成分分析 聚类分析
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Using genetic algorithm based fuzzy adaptive resonance theory for clustering analysis 被引量:3
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作者 LIU Bo WANG Yong WANG Hong-jian 《哈尔滨工程大学学报》 EI CAS CSCD 北大核心 2006年第B07期547-551,共5页
关键词 聚类分析 遗传算法 模糊自适应谐振理论 人工神经网络
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The Definition of Sustainable Development of Private University- Based on the Method of Literature Review and Cluster Analysis 被引量:1
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作者 Shi Meng Wang Dongling Dai Jiabao(译) 《学术界》 CSSCI 北大核心 2016年第2期314-319,共6页
The sustainable development of private university has become the focus of the academia and the private higher education after an approximately golden period.Through the method of literature review and cluster analysis... The sustainable development of private university has become the focus of the academia and the private higher education after an approximately golden period.Through the method of literature review and cluster analysis,this paper studies the concept of sustainable development of private university from the perspective of the connotation definition and epitaxial recognition,in order to effectively reveal the essence of the sustainable development of private university,hoping to provide some certain support for the theory and practice of sustainable development of private university. 展开更多
关键词 可持续发展 民办高校 聚类分析 文献综述 定义 教育发展 学术界
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Insulation Diagnosis of Service Aged XLPE Power Cables Using Statistical Analysis and Fuzzy Inference 被引量:1
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作者 LIU Fei JIANG Pingkai +2 位作者 LEI Qingquan ZHANG Li SU Wenqun 《高电压技术》 EI CAS CSCD 北大核心 2013年第8期1932-1940,共9页
Cables that have been in service for over 20 years in Shanghai, a city with abundant surface water, failed more frequently and induced different cable accidents. This necessitates researches on the insulation aging st... Cables that have been in service for over 20 years in Shanghai, a city with abundant surface water, failed more frequently and induced different cable accidents. This necessitates researches on the insulation aging state of cables working in special circumstances. We performed multi-parameter tests with samples from about 300 cable lines in Shanghai. The tests included water tree investigation, tensile test, dielectric spectroscopy test, thermogravimetric analysis (TGA), fourier transform infrared spectroscopy (FTIR), and electrical aging test. Then, we carried out regression analysis between every two test parameters. Moreover, through two-sample t-Test and analysis of va- riance (ANOVA) of each test parameter, we analyzed the influences of cable-laying method and sampling section on the degradation of cable insulation respectively. Furthermore, the test parameters which have strong correlation in the regression analysis or significant differ- ences in the t-Test or ANOVA analysis were determined to be the ones identifying the XLPE cable insulation aging state. The thresholds for distinguishing insulation aging states had been also obtained with the aid of statistical analysis and fuzzy clustering. Based on the fuzzy in- ference, we established a cable insulation aging diagnosis model using the intensity transfer method. The results of regression analysis indicate that the degradation of cable insulation accelerates as the degree of in-service aging increases. This validates the rule that the in- crease of microscopic imperfections in solid material enhances the dielectric breakdown strength. The results of the two-sample t-Test and the ANOVA indicate that the direct-buried cables are more sensitive to insulation degradation than duct cables. This confirms that the tensile strength and breakdown strength are reliable functional parameters in cable insulation evaluations. A case study further indicates that the proposed diagnosis model based on the fuzzy inference can reflect the comprehensive aging state of cable insulation well, and that the cable service time has no correlation with the insulation aging state. 展开更多
关键词 交联聚乙烯绝缘电力电缆 模糊推理 统计分析 诊断服务 傅立叶变换红外光谱 电缆绝缘层 老化状态 电击穿强度
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基于CRISPR/Cas9技术创制高维生素C番茄材料
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作者 安煊森 王雁伟 +2 位作者 田文超 任亚娟 艾鹏飞 《中国生物化学与分子生物学报》 北大核心 2025年第3期460-469,共10页
组成型光形态建成9信号复合体的5B亚基(subunit 5B of constitutively photomorphogenic 9 signalosome,CSN5B)是植物中L-半乳糖途径合成维生素C的抑制因子。为了获得高维生素C番茄突变体,本文构建双靶点载体pKSE402-SlCSN5B并对番茄育... 组成型光形态建成9信号复合体的5B亚基(subunit 5B of constitutively photomorphogenic 9 signalosome,CSN5B)是植物中L-半乳糖途径合成维生素C的抑制因子。为了获得高维生素C番茄突变体,本文构建双靶点载体pKSE402-SlCSN5B并对番茄育种亲本“1912”进行了遗传转化。通过分子生物学鉴定和DNA序列分析,17株转基因阳性植株中6株发生编辑,编辑效率为35.3%;其中csn5b-6和csn5b-8为纯合突变体,分别缺失了180 bp和3 bp。对这2种缺失纯合体T 1代中无外源T-DNA插入的株系分别进行生物学观测,在植株和果实的表型上无明显差异;在果实红熟期,对其进行生理指标测定,csn5b-6的T 1代株系在GMPase活力、维生素C和过氧化氢含量变化显著,分别比野生型提高43%、37.8%和降低25.9%,而可溶性固形物含量无明显差异;csn5b-8的T 1代株系与野生型一致。采用基因表达分析和蛋白质结构预测发现,csn5b-6-11中SlCSN5B基因表达量正常,但其编码的蛋白质由于较大的肽段丢失引起结构的改变,从而影响其功能,这可能是引起维生素C含量提高的原因。以上结果表明,利用CRISPR/Cas9技术编辑SlCSN5B基因可以提高番茄果实中维生素C的含量,为后续培育优质番茄新品种提供材料。 展开更多
关键词 CRISPR/cas9 番茄 SlCSN5B 维生素C
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Non-Isothermal Kinetic Analysis of Thermal Decomposition of the Ca-Bentonite from Santai, China
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作者 ZHANG Xianghui HE Chuan +3 位作者 WANG Ling DENG Miao LIU Jing FENG Qian 《矿物学报》 CAS CSCD 北大核心 2013年第S1期138-138,共1页
The thermal decompositions of Ca-bentonites (CaB) from Santai ,Shichuan Province, China over the temperature rage of 30-1100℃ were investigated by simultaneous thermal analyzer. Non-isothermal Kinetic analysis were e... The thermal decompositions of Ca-bentonites (CaB) from Santai ,Shichuan Province, China over the temperature rage of 30-1100℃ were investigated by simultaneous thermal analyzer. Non-isothermal Kinetic analysis were employed to study the thermal decomposition mechanism by using Netzsch Thermokinetics software. The dependence of the activation energy on conversion degree were evaluated by isoconversional methods. The probably mechanism and the corresponding kinetic parameters were determined by multivariate non-linear regression program. 展开更多
关键词 NON-ISOTHERMAL KINETIC SIMULTANEOUS THERMAL analysis ca-BENTONITE THERMAL d
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Clustering Analysis of Black-start Decision-making with a Large Group of Decision-makers
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作者 Liu, Weijia Lin, Zhenzhi +4 位作者 Wen, Fushuan Xue, Yusheng Dai, Yan Sun, Weizhen Wang, Chao 《电力系统自动化》 EI CSCD 北大核心 2012年第8期154-160,共7页
The optimization of black-start decision-making plays an important role in the rapid restoration of a power system after a major failure/outage.With the introduction of the concept of smart grids and the development o... The optimization of black-start decision-making plays an important role in the rapid restoration of a power system after a major failure/outage.With the introduction of the concept of smart grids and the development of real-time communication networks,the black-start decision-makers are no longer limited to only one or a few power system experts such as dispatchers,but rather a large group of professional people in practice.The overall behaviors of a large decision-making group of decision-makers/experts are more complicated and unpredictable.However,the existing methods for black-start decision-making cannot handle the situations with a large group of decision-makers.Given this background,a clustering algorithm is presented to optimize the black-start decision-making problem with a large group of decision-makers.Group decision-making preferences are obtained by clustering analysis,and the final black-start decision-making results are achieved by combining the weights of black-start indexes and the preferences of the decision-making group.The effectiveness of the proposed method is validated by a practical case.This work extends the black-start decision-making problem to situations with a large group of decision-makers. 展开更多
关键词 决策者 聚类分析 黑启动 大集 实时通信网络 决策问题 电力系统 电源系统
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基于PCA-Clustering的压缩机回液故障诊断 被引量:10
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作者 周镇新 李绍斌 +5 位作者 谭泽汉 陈焕新 王江宇 刘江岩 郭亚宾 孙劭波 《制冷学报》 CAS CSCD 北大核心 2018年第4期111-118,共8页
在多联机(VRF)空调系统中,压缩机回液将导致能量损失。本文结合大数据提出了一种基于PCA-Clustering的压缩机回液故障诊断的方法。首先提取出故障相关变量,并通过数据预处理,剔除异常值与空值;然后将处理后的数据进行主成分分析(PCA),... 在多联机(VRF)空调系统中,压缩机回液将导致能量损失。本文结合大数据提出了一种基于PCA-Clustering的压缩机回液故障诊断的方法。首先提取出故障相关变量,并通过数据预处理,剔除异常值与空值;然后将处理后的数据进行主成分分析(PCA),获取降维后的新主元变量数据;最后将新的主元变量进行聚类分析(Clustering analysis)得到回液故障数据分类标签。结果表明:该方法能够在数据标签未知的情况下,较好的区分不同类别的压缩机回液故障及正常数据,使压缩机回液故障诊断率达到94.29%。 展开更多
关键词 多联机系统 压缩机回液 故障检测与诊断 聚类分析 主成分分析
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RAPD analysis on the four kinds of color-flowered Salvia splendens Ker-Gawl 被引量:1
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作者 HU Guofu YUAN Qiang LI Fenglan WEI Qi HU Baozhong 《Journal of Northeast Agricultural University(English Edition)》 CAS 2007年第2期97-102,共6页
In this study, we used RAPD to analyze four kinds of color-flowered Salvia splendens Ker-Gawl, and the optimal RAPD reaction conditions were the optimal reaction mixture (25 μL total volume) that contained 2.0 μL ... In this study, we used RAPD to analyze four kinds of color-flowered Salvia splendens Ker-Gawl, and the optimal RAPD reaction conditions were the optimal reaction mixture (25 μL total volume) that contained 2.0 μL 10×buffer, 0.45 mmol·L^-1 dNTPs, 2.0 mmol· L^-1 Mg^2+, 2 U Taq DNA polymerase, 0.30 umol·L^-2 primer and 40 ng genomic DNA. Total 84 bands were amplified from 12 primers used, and the differential bands had 28 bands, which was 33% of total bands. In cluster group analysis, the four kinds of color-flowered were divided into two styles. One style is that the red color and red-white color were grouped together, then they grouped with purple color into one cluster, and the white color was another style. 展开更多
关键词 Salvia splendens Ker-Gawl COLOR RAPD analysis cluster group analysis
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基于CRISPR/Cas系统的纸基分析在快速检测食源性致病菌中的应用 被引量:2
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作者 李鹏儒 沈兴 +3 位作者 孟静南 罗林 王涓 徐振林 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2024年第5期1147-1160,共14页
由食源性致病菌引起的食品安全事件严重影响人类健康,开发针对食源性致病菌的快速检测技术十分必要。成簇间隔短回文重复序列(clustered regularly interspaced short palindromic repeats,CRISPR)及相关蛋白(CRISPR-associated protein... 由食源性致病菌引起的食品安全事件严重影响人类健康,开发针对食源性致病菌的快速检测技术十分必要。成簇间隔短回文重复序列(clustered regularly interspaced short palindromic repeats,CRISPR)及相关蛋白(CRISPR-associated protein,Cas)是原核生物的适应性免疫系统,具有特异性识别并切割核酸序列的功能。纸基分析方法作为一种简便性好、成本低廉的分析检测工具,在快速检测领域展现出良好的前景。因此,将CRISPR/Cas系统的高效识别能力和纸基分析方法的简便性相结合可实现对食源性致病菌的快速灵敏检测。本文简要介绍了CRISPR/Cas系统用于核酸检测的概况,对第二类单Cas效应蛋白系统的特点及原理进行概述,重点综述基于CRISPR/Cas系统的试纸分析、侧向流动分析和纸基微流控装置在检测食源性致病菌方面的应用,并讨论了CRISPR/Cas系统结合纸基分析建立检测方法的优势、当前的挑战及未来的发展前景。 展开更多
关键词 成簇间隔短回文重复序列及相关蛋白 食源性致病菌 纸基分析 快速检测
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CRISPR/Cas系统中工程化gRNA技术的研究和应用 被引量:1
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作者 谈鎏 叶邦策 尹斌成 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2024年第8期1078-1092,共15页
CRISPR/Cas是原核生物在进化过程中获得的一种免疫防御系统,用于抵抗外来遗传物质的入侵,近年来被开发成为高效的基因编辑、基因调控以及分子诊断工具。其可编程靶向机制揭开了利用该系统进行基因组操作的序幕,并允许在活性范围内实现... CRISPR/Cas是原核生物在进化过程中获得的一种免疫防御系统,用于抵抗外来遗传物质的入侵,近年来被开发成为高效的基因编辑、基因调控以及分子诊断工具。其可编程靶向机制揭开了利用该系统进行基因组操作的序幕,并允许在活性范围内实现动态调节和控制基因表达。作为现有基因修饰手段中灵活性最强和成本最低的技术之一,已被广泛应用于临床疾病治疗、工农业生产、可持续染料开发和化学品加工等领域。随着对CRISPR/Cas系统的不断深入挖掘和探索,大量研究报道了gRNA工程改造及优化方法,包括改变间隔序列长度、调节恒定区和可变区的结构、向末端或中间延伸添加额外功能序列及化学合成修饰等,以期降低脱靶突变率,提高作用效率,充分激发CRISPR基因操纵工具在生物医学方面的潜力。基于此,本综述将介绍CRISPR/Cas9和CRISPR/Cas12系统中gRNA工程化设计方法及应用研究的最新进展,分析探讨了当前工程化gRNA技术面临的机遇和挑战,旨在为获得性能更加优异的gRNA提供思路和方向,从而提高利用CRISPR/Cas系统探测人类细胞基因组的能力,进一步为可编程生物学带来更多可能性。 展开更多
关键词 CRISPR/cas系统 gRNA工程化 基因编辑 特异性
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一种基于EGK-均值聚类和PCA的海流发电机叶片冲击故障检测方法 被引量:4
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作者 谢涛 张卫东 张义博 《太阳能学报》 EI CAS CSCD 北大核心 2024年第3期201-209,共9页
海流发电机受海流流速变化、随机湍流的影响呈现多变工况,想要准确检测出叶片冲击故障是一项巨大挑战。基于此,提出一种基于包络几何特征聚类(EGK-均值聚类)的方法用于划分变工况下产生的定子电流信号,在不同子工况下建立故障检测模型... 海流发电机受海流流速变化、随机湍流的影响呈现多变工况,想要准确检测出叶片冲击故障是一项巨大挑战。基于此,提出一种基于包络几何特征聚类(EGK-均值聚类)的方法用于划分变工况下产生的定子电流信号,在不同子工况下建立故障检测模型。首先,根据样本矩阵构建包络几何特征矩阵,利用几何特征矩阵确定初始聚类中心进行聚类;然后,基于各工况下的包络数据分别进行主元分析以降低数据维度;最后,T^(2)和SPE统计量被计算用于实时冲击故障检测。一台海流发电机样机及配套循环水槽被搭建验证本文所提方法。实验结果表明本文所提方法在变工况下针对海流发电机叶片冲击故障有着显著的识别能力和检测精度。 展开更多
关键词 海洋能 海洋工程 信号处理 聚类算法 故障检测 主成分分析
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Genetic Diversity Analysis of Aneurolepidium chinense (Trin) Kitag by ISSR Marker
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作者 YIN Zhi,LI Chengyan,LI Hechun,and HU Guofu College of Life Sciences,Northeast Agricultural University,Harbin 150030,China 《Journal of Northeast Agricultural University(English Edition)》 CAS 2010年第2期55-59,共5页
In the study,the reasonable sampling of the grey Aneurolepidium chinense of green grassland,the grey-green A.chinense of green grassland,the grey A.chinense of Wulimu and the grey-green A.chinense of Wulimu were analy... In the study,the reasonable sampling of the grey Aneurolepidium chinense of green grassland,the grey-green A.chinense of green grassland,the grey A.chinense of Wulimu and the grey-green A.chinense of Wulimu were analyzed by ISSR.Eight primers with clear and diverse products were screened out from 20 primers and 47 DNA fragments were amplified from 39 individuals.The average number of DNA fragments produced by each primer was 5.9,and polymorphic bands were 41 and the polymorphic rate was 87.23%,which could r... 展开更多
关键词 Aneurolepidium chinense (Trin) Kitag ISSR genetic variation cluster analysis
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