目的:研究P38丝裂原活化蛋白激酶(P38mitogen-activated protein kinases,P38MAPK)通路在骨形态发生蛋白9(Bonemorphogenetic protein 9,BMP9)诱导C3H10T1/2干细胞向心肌样细胞分化中的作用。方法:免疫印迹检测经pAdEasyBMP9诱导24 h的C...目的:研究P38丝裂原活化蛋白激酶(P38mitogen-activated protein kinases,P38MAPK)通路在骨形态发生蛋白9(Bonemorphogenetic protein 9,BMP9)诱导C3H10T1/2干细胞向心肌样细胞分化中的作用。方法:免疫印迹检测经pAdEasyBMP9诱导24 h的C3H10T1/2干细胞磷酸化P38MAPK和P38MAPK表达水平,免疫荧光定位磷酸化P38MAPK;SB203580抑制C3H10T1/2干细胞磷酸化P38MAPK活性,pAdEasyBMP9诱导21 d时免疫印迹检测心肌特异性蛋白CX43、cTnT表达,免疫荧光检测心肌特异性蛋白cTnT、α-MHC表达,RT-qPCR检测心肌特异性基因GATA4、MEF2C表达。结果:pAdEasyBMP9促进磷酸化P38MAPK表达增高,却不影响P38MAPK表达水平;磷酸化P38MAPK有表达于胞浆和胞核、仅表达于胞核的不同状态。SB203580可显著性地抑制由pAdEasyBMP9诱导的C3H10T1/2干细胞CX43、cTnT、α-MHC、GATA4、MEF2C表达。结论:pAdEasyBMP9诱导C3H10T1/2干细胞能激活P38MAPK通路,并促进其向心肌样细胞分化。展开更多
The Complex Co〔S 2P(O- i -C 3H 7) 2〕 3 was synthesized through the reaction of Co 2(CO) 8 with the ligand (C 3H 7- i -O) 2P(S)S-SP(S)(O- i -C 3H 7) 2,and its crystal structure was determined by X-ray four -cycle dif...The Complex Co〔S 2P(O- i -C 3H 7) 2〕 3 was synthesized through the reaction of Co 2(CO) 8 with the ligand (C 3H 7- i -O) 2P(S)S-SP(S)(O- i -C 3H 7) 2,and its crystal structure was determined by X-ray four -cycle diffraction method.The crystal belongs to triclinic,space group Pi,crystal cell parameters:a=9 009(2),b=10 437(2),c=18 858(4)?,α=80 99(3),β=88 55(3),γ=82 01(3)°,V=1734 3(6)? 3,Z=2,Mr=698 7,Dc=1 338g·cm -3 ,μ=1 022mm -1 ,F(000)=732,R=0 0342,Rw=0 0812.The structural analysis showed that six sulfur atoms from three ligands 〔S 2P(O- i -C 3H 7) 2〕 coordinate with a cobalt atom,CoS 6 framework in molecular stucture approximately octahedral.The complex was also characterized by IR? 1H NMR? 31 P NMR and MS.展开更多
文摘目的:研究P38丝裂原活化蛋白激酶(P38mitogen-activated protein kinases,P38MAPK)通路在骨形态发生蛋白9(Bonemorphogenetic protein 9,BMP9)诱导C3H10T1/2干细胞向心肌样细胞分化中的作用。方法:免疫印迹检测经pAdEasyBMP9诱导24 h的C3H10T1/2干细胞磷酸化P38MAPK和P38MAPK表达水平,免疫荧光定位磷酸化P38MAPK;SB203580抑制C3H10T1/2干细胞磷酸化P38MAPK活性,pAdEasyBMP9诱导21 d时免疫印迹检测心肌特异性蛋白CX43、cTnT表达,免疫荧光检测心肌特异性蛋白cTnT、α-MHC表达,RT-qPCR检测心肌特异性基因GATA4、MEF2C表达。结果:pAdEasyBMP9促进磷酸化P38MAPK表达增高,却不影响P38MAPK表达水平;磷酸化P38MAPK有表达于胞浆和胞核、仅表达于胞核的不同状态。SB203580可显著性地抑制由pAdEasyBMP9诱导的C3H10T1/2干细胞CX43、cTnT、α-MHC、GATA4、MEF2C表达。结论:pAdEasyBMP9诱导C3H10T1/2干细胞能激活P38MAPK通路,并促进其向心肌样细胞分化。
文摘The Complex Co〔S 2P(O- i -C 3H 7) 2〕 3 was synthesized through the reaction of Co 2(CO) 8 with the ligand (C 3H 7- i -O) 2P(S)S-SP(S)(O- i -C 3H 7) 2,and its crystal structure was determined by X-ray four -cycle diffraction method.The crystal belongs to triclinic,space group Pi,crystal cell parameters:a=9 009(2),b=10 437(2),c=18 858(4)?,α=80 99(3),β=88 55(3),γ=82 01(3)°,V=1734 3(6)? 3,Z=2,Mr=698 7,Dc=1 338g·cm -3 ,μ=1 022mm -1 ,F(000)=732,R=0 0342,Rw=0 0812.The structural analysis showed that six sulfur atoms from three ligands 〔S 2P(O- i -C 3H 7) 2〕 coordinate with a cobalt atom,CoS 6 framework in molecular stucture approximately octahedral.The complex was also characterized by IR? 1H NMR? 31 P NMR and MS.
