Background: T-2 toxin poses a great threat to human health because it has the highest toxicity of the currently known trichothecene mycotoxins. To understand the in vivo toxicity and transformation mechanism of T-2 to...Background: T-2 toxin poses a great threat to human health because it has the highest toxicity of the currently known trichothecene mycotoxins. To understand the in vivo toxicity and transformation mechanism of T-2 toxin, we investigated the role of two principal phase Ⅰ drug-metabolizing enzymes(cytochrome P450 [CYP450] enzymes) on the metabolism of T-2 toxin, which are crucial to the metabolism of endogenous substances and xenobiotics. We also investigated carboxylesterase, which also plays an important role in the metabolism of toxic substances.Methods: A chemical inhibition method and a recombinant method were employed to investigate the metabolism of the T-2 toxin by the CYP450 enzymes, and a chemical inhibition method was used to study carboxylesterase metabolism. Samples incubated with human liver microsomes were analyzed by high performance liquid chromatography-triple quadrupole mass spectrometry(HPLC- Qq Q MS) after a simple pretreatment.Results: In the presence of a carboxylesterase inhibitor, only 20% T-2 toxin was metabolized. When CYP enzyme inhibitors and a carboxylesterase inhibitor were both present, only 3% of the T-2 toxin was metabolized. The contributions of the CYP450 enzyme family to T-2 toxin metabolism followed the descending order CYP3A4, CYP2E1, CYP1A2, CYP2B6 or CYP2D6 or CYP2C19.Conclusions: Carboxylesterase and CYP450 enzymes are of great importance in T-2 toxin metabolism, in which carboxylesterase is predominant and CYP450 has a subordinate role. CYP3A4 is the principal member of the CYP450 enzyme family responsible for T-2 toxin metabolism. The metabolite produced by carboxylesterase is HT-2, and the metabolite produced by CYP 3A4 is 3'-OH T-2. The different metabolites show different toxicities. Our results will provide useful data concerning the toxic mechanism, the safety evaluation, and the health risk assessment of T-2 toxin.展开更多
Glioblastoma multiforme(GBM)is the most common malignant primary brain tumor with a poor prognosis and limited survival.Patients with GBM have a high demand for palliative care.In our present case,a 21-year-old female...Glioblastoma multiforme(GBM)is the most common malignant primary brain tumor with a poor prognosis and limited survival.Patients with GBM have a high demand for palliative care.In our present case,a 21-year-old female GBM patient received inpatient palliative care services including symptom management,mental and psychological support for the patient,psychosocial and clinical decision support for her family members,and pre-and post-death bereavement management for the family.Furthermore,we provided the family members with comprehensive psychological preparation for the patient's demise and assisted the patient's family throughout the mourning period.The aim of this study is to provide a reference and insights for the clinical implementation of palliative care for patients with malignant brain tumors.展开更多
The growing utilization of critical care echocardiography(CCE) by clinicians necessitates a meticulous review of clinical conditions in critically ill patients, both before and during the examination. The reviewing pr...The growing utilization of critical care echocardiography(CCE) by clinicians necessitates a meticulous review of clinical conditions in critically ill patients, both before and during the examination. The reviewing process of clinical conditions minimizes the risk of overlooking or misinterpreting crucial findings. This article proposes a comprehensive strategy, namely BILL strategy, to integrate into the CCE protocol, where "B" represents baseline respiratory and hemodynamic support, "I" signifies information gleaned from invasive monitoring, including central venous pressure and thermodilution-derived cardiac output, the first "L" denotes laboratory results such as central venous oxygen saturation, troponin, and brain natriuretic peptide, and the second "L" refers to lung ultrasound data. Combining the BILL strategy with CCE can enhance comprehensive understanding of critical conditions, potentially leading to improved diagnostic accuracy and patient outcomes.展开更多
Approximately 40% of pheochromocytoma and paraganglioma(PPGL) cases are familial, typically presenting earlier with more complex symptoms. This paper synthesizes literature and guidelines to inform on clinical charact...Approximately 40% of pheochromocytoma and paraganglioma(PPGL) cases are familial, typically presenting earlier with more complex symptoms. This paper synthesizes literature and guidelines to inform on clinical characteristics and perioperative care for PPGL. Pheochromocytoma in von Hippel-Lindau(VHL) disease exhibits heightened secretion activity without significant perioperative hemodynamic changes. Tumors in multiple endocrine neoplasia type 2(MEN2) have a stronger endocrine function, which may induce hemodynamic fluctuations during surgery. Therefore, pheochromocytoma screening is essential at all stages of MEN2. Neurofibromatosis type 1(NF1) often presents multisystem lesions and can result in difficult airway. Pheochromocytoma should be evaluated when NF1 patients present hypertension. Pheochromocytoma and paraganglioma type 5 may present multiple lesions of pheochromocytoma or paraganglioma. In summary, hereditary PPGLs may present with severe lesions in other systems, beyond tumor function. A multi-disciplinary team(MDT) approach is often invaluable in perioperative management.展开更多
BACKGROUND:The objective of this retrospective hospital-based study was to describe the epidemiological features of traumatic spinal cord injury(TSCI)in the intensive care unit(ICU)and assess the incidence and possibl...BACKGROUND:The objective of this retrospective hospital-based study was to describe the epidemiological features of traumatic spinal cord injury(TSCI)in the intensive care unit(ICU)and assess the incidence and possible risk factors for venous thromboembolism(VTE)following TSCI.METHODS:We retrospectively reviewed the medical records of 370 patients with TSCI who were admitted between January 2018 and March 2023.The following parameters were collected:age,sex,body mass index,occupation,underlying diseases,smoking history,education level,etiology of injury,injury segments,American Spinal Injury Association(ASIA)Impairment Scale score,severity of injury,injury severity score(ISS),VTE risk score(Caprini score),treatment,VTE prophylaxis,ICU length of stay,length of hospital stay,concomitant injuries,and complications.Descriptive statistics were used to summarize the demographic and clinical characteristics of the study participants.Logistic regression analysis was used to determine the risk factors for VTE.RESULTS:The mean age of patients with TSCI was 55.5±13.4 years,with a male-to-female ratio of 6.5:1.The leading cause of TSCI was falls from height(46.5%),followed by traffic accidents(36.5%).The cervical spinal cord was the most affected segment,followed by the thoracolumbar region.Among all the patients,362(97.8%)had concomitant injuries.Complications were observed in 255 patients(68.9%)during hospitalization.The incidence rate of VTE was 25.1%.Logistic regression analysis revealed that age(OR=1.721,95%CI:1.207-2.454,P=0.003),mechanical ventilation(OR=3.427,95%CI:1.873-6.271,P<0.001),and non-use of chemical prophylaxis(OR=2.986,95%CI:1.749-5.099,P<0.001)were risk factors for VTE.CONCLUSION:Falls from height and traffic accidents were the main causes of TSCIs in the ICU,especially for male patients with cervical spinal cord injuries.VTE is a frequent complication in patients with TSCI in the ICU.Age,mechanical ventilation,and non-use of chemical prophylaxis were found to be independent risk factors for VTE following TSCI.展开更多
目的对比分析2012—2022年中国学术期刊网络出版总库(CNKI)和Web of Science(WOS)数据库中脑卒中延续护理领域的研究差异。方法分别以CNKI和WOS数据库2012年1月1日至2022年12月31日收录的脑卒中延续护理领域文献为研究对象,应用CiteSpac...目的对比分析2012—2022年中国学术期刊网络出版总库(CNKI)和Web of Science(WOS)数据库中脑卒中延续护理领域的研究差异。方法分别以CNKI和WOS数据库2012年1月1日至2022年12月31日收录的脑卒中延续护理领域文献为研究对象,应用CiteSpace可视化软件对纳入文献进行分析,以确定国内外脑卒中延续护理相关研究的发展脉络及研究热点和研究趋势,并分析其差异。结果国内外对脑卒中延续护理方面的研究差异较大,国内的研究者中心性不高,主要研究团队相对分散,彼此间联系不够紧密,国外部分作者中心性相对较高,研究团队相对集中,但彼此之间合作也不够紧密。国内发文机构比较多,发文量最多的机构为郑州大学护理与健康学院,但各个机构间的合作不够紧凑。国外发文量最多的机构为多伦多大学,机构之间合作紧密,其中凯撒医院具有较高的影响力。国内的研究热点主要集中在对个体尤其是老年患者生理、心理及遵医依从性方面的影响,倾向于干预结果。国外研究热点更多地关注脑卒中患者的二级预防及治疗措施方面,探讨如何更好地从源头促进脑卒中患者的康复,倾向于过程及措施方面。国内的研究前沿主要集中在通过多媒体互联网平台来提高恢复期、偏瘫患者的自理能力等方面;国外的研究前沿集中在从源头切断病因更好地提高脑卒中患者的生存质量,减轻预后护理负担,但是对于脑卒中延续护理平台的选择依旧是趋于传统。结论脑卒中延续护理领域正处在快速发展阶段,我国应进一步加大研究力度,拓宽合作领域,聚焦相关研究热点及前沿,从国外研究中汲取精华,以更好地促进我国脑卒中延续护理的发展。展开更多
目的探讨BirthRite坐位分娩组件接产的安全性和有效性,为缩短初产妇第二产程时间,提升产妇生产体验提供参考。方法将住院分娩的低危初产妇236例,第二产程按照产妇意愿分为观察组(116例)和对照组(120例)。观察组采用BirthRite坐位分娩组...目的探讨BirthRite坐位分娩组件接产的安全性和有效性,为缩短初产妇第二产程时间,提升产妇生产体验提供参考。方法将住院分娩的低危初产妇236例,第二产程按照产妇意愿分为观察组(116例)和对照组(120例)。观察组采用BirthRite坐位分娩组件坐式分娩,对照组采用电动产床坐式分娩。观察两组产妇第二产程时间、非枕前位占比、阴道助产、产后出血、肩难产、会阴侧切、会阴裂伤、会阴水肿、会阴疼痛程度和产后满意度;比较两组新生儿出生体质量和1 min、5 min Apgar评分。结果对照组中途2例退出。两组均顺利分娩,观察组第二产程时间显著短于对照组,分娩非枕前位占比、肩难产发生率、会阴侧切率、会阴水肿及疼痛程度显著低于对照组,产妇满意度显著高于对照组(均P<0.05)。两组新生儿出生后1 min和5 min Apgar评分均为10分。结论使用BirthRite分娩椅能够缩短第二产程时间,提高产妇满意度,优化坐位分娩结局。展开更多
基金supported by the Key Projects in the National Science & Technology Pillar Program of China (2011BAK10B07)the National Major Special Projects in the Ministry of Science and Technology of China (2012 2X09301003-001-010)
文摘Background: T-2 toxin poses a great threat to human health because it has the highest toxicity of the currently known trichothecene mycotoxins. To understand the in vivo toxicity and transformation mechanism of T-2 toxin, we investigated the role of two principal phase Ⅰ drug-metabolizing enzymes(cytochrome P450 [CYP450] enzymes) on the metabolism of T-2 toxin, which are crucial to the metabolism of endogenous substances and xenobiotics. We also investigated carboxylesterase, which also plays an important role in the metabolism of toxic substances.Methods: A chemical inhibition method and a recombinant method were employed to investigate the metabolism of the T-2 toxin by the CYP450 enzymes, and a chemical inhibition method was used to study carboxylesterase metabolism. Samples incubated with human liver microsomes were analyzed by high performance liquid chromatography-triple quadrupole mass spectrometry(HPLC- Qq Q MS) after a simple pretreatment.Results: In the presence of a carboxylesterase inhibitor, only 20% T-2 toxin was metabolized. When CYP enzyme inhibitors and a carboxylesterase inhibitor were both present, only 3% of the T-2 toxin was metabolized. The contributions of the CYP450 enzyme family to T-2 toxin metabolism followed the descending order CYP3A4, CYP2E1, CYP1A2, CYP2B6 or CYP2D6 or CYP2C19.Conclusions: Carboxylesterase and CYP450 enzymes are of great importance in T-2 toxin metabolism, in which carboxylesterase is predominant and CYP450 has a subordinate role. CYP3A4 is the principal member of the CYP450 enzyme family responsible for T-2 toxin metabolism. The metabolite produced by carboxylesterase is HT-2, and the metabolite produced by CYP 3A4 is 3'-OH T-2. The different metabolites show different toxicities. Our results will provide useful data concerning the toxic mechanism, the safety evaluation, and the health risk assessment of T-2 toxin.
基金National High Level Hospital Clinical Research Funding(2022-PUMCH-B-113).
文摘Glioblastoma multiforme(GBM)is the most common malignant primary brain tumor with a poor prognosis and limited survival.Patients with GBM have a high demand for palliative care.In our present case,a 21-year-old female GBM patient received inpatient palliative care services including symptom management,mental and psychological support for the patient,psychosocial and clinical decision support for her family members,and pre-and post-death bereavement management for the family.Furthermore,we provided the family members with comprehensive psychological preparation for the patient's demise and assisted the patient's family throughout the mourning period.The aim of this study is to provide a reference and insights for the clinical implementation of palliative care for patients with malignant brain tumors.
文摘The growing utilization of critical care echocardiography(CCE) by clinicians necessitates a meticulous review of clinical conditions in critically ill patients, both before and during the examination. The reviewing process of clinical conditions minimizes the risk of overlooking or misinterpreting crucial findings. This article proposes a comprehensive strategy, namely BILL strategy, to integrate into the CCE protocol, where "B" represents baseline respiratory and hemodynamic support, "I" signifies information gleaned from invasive monitoring, including central venous pressure and thermodilution-derived cardiac output, the first "L" denotes laboratory results such as central venous oxygen saturation, troponin, and brain natriuretic peptide, and the second "L" refers to lung ultrasound data. Combining the BILL strategy with CCE can enhance comprehensive understanding of critical conditions, potentially leading to improved diagnostic accuracy and patient outcomes.
文摘Approximately 40% of pheochromocytoma and paraganglioma(PPGL) cases are familial, typically presenting earlier with more complex symptoms. This paper synthesizes literature and guidelines to inform on clinical characteristics and perioperative care for PPGL. Pheochromocytoma in von Hippel-Lindau(VHL) disease exhibits heightened secretion activity without significant perioperative hemodynamic changes. Tumors in multiple endocrine neoplasia type 2(MEN2) have a stronger endocrine function, which may induce hemodynamic fluctuations during surgery. Therefore, pheochromocytoma screening is essential at all stages of MEN2. Neurofibromatosis type 1(NF1) often presents multisystem lesions and can result in difficult airway. Pheochromocytoma should be evaluated when NF1 patients present hypertension. Pheochromocytoma and paraganglioma type 5 may present multiple lesions of pheochromocytoma or paraganglioma. In summary, hereditary PPGLs may present with severe lesions in other systems, beyond tumor function. A multi-disciplinary team(MDT) approach is often invaluable in perioperative management.
基金supported in part by grants from the Science and Technology Plan Project of Linhai(2023YW05)Medical Health Science and Technology Project of Zhejiang(2024KY555).
文摘BACKGROUND:The objective of this retrospective hospital-based study was to describe the epidemiological features of traumatic spinal cord injury(TSCI)in the intensive care unit(ICU)and assess the incidence and possible risk factors for venous thromboembolism(VTE)following TSCI.METHODS:We retrospectively reviewed the medical records of 370 patients with TSCI who were admitted between January 2018 and March 2023.The following parameters were collected:age,sex,body mass index,occupation,underlying diseases,smoking history,education level,etiology of injury,injury segments,American Spinal Injury Association(ASIA)Impairment Scale score,severity of injury,injury severity score(ISS),VTE risk score(Caprini score),treatment,VTE prophylaxis,ICU length of stay,length of hospital stay,concomitant injuries,and complications.Descriptive statistics were used to summarize the demographic and clinical characteristics of the study participants.Logistic regression analysis was used to determine the risk factors for VTE.RESULTS:The mean age of patients with TSCI was 55.5±13.4 years,with a male-to-female ratio of 6.5:1.The leading cause of TSCI was falls from height(46.5%),followed by traffic accidents(36.5%).The cervical spinal cord was the most affected segment,followed by the thoracolumbar region.Among all the patients,362(97.8%)had concomitant injuries.Complications were observed in 255 patients(68.9%)during hospitalization.The incidence rate of VTE was 25.1%.Logistic regression analysis revealed that age(OR=1.721,95%CI:1.207-2.454,P=0.003),mechanical ventilation(OR=3.427,95%CI:1.873-6.271,P<0.001),and non-use of chemical prophylaxis(OR=2.986,95%CI:1.749-5.099,P<0.001)were risk factors for VTE.CONCLUSION:Falls from height and traffic accidents were the main causes of TSCIs in the ICU,especially for male patients with cervical spinal cord injuries.VTE is a frequent complication in patients with TSCI in the ICU.Age,mechanical ventilation,and non-use of chemical prophylaxis were found to be independent risk factors for VTE following TSCI.
文摘目的对比分析2012—2022年中国学术期刊网络出版总库(CNKI)和Web of Science(WOS)数据库中脑卒中延续护理领域的研究差异。方法分别以CNKI和WOS数据库2012年1月1日至2022年12月31日收录的脑卒中延续护理领域文献为研究对象,应用CiteSpace可视化软件对纳入文献进行分析,以确定国内外脑卒中延续护理相关研究的发展脉络及研究热点和研究趋势,并分析其差异。结果国内外对脑卒中延续护理方面的研究差异较大,国内的研究者中心性不高,主要研究团队相对分散,彼此间联系不够紧密,国外部分作者中心性相对较高,研究团队相对集中,但彼此之间合作也不够紧密。国内发文机构比较多,发文量最多的机构为郑州大学护理与健康学院,但各个机构间的合作不够紧凑。国外发文量最多的机构为多伦多大学,机构之间合作紧密,其中凯撒医院具有较高的影响力。国内的研究热点主要集中在对个体尤其是老年患者生理、心理及遵医依从性方面的影响,倾向于干预结果。国外研究热点更多地关注脑卒中患者的二级预防及治疗措施方面,探讨如何更好地从源头促进脑卒中患者的康复,倾向于过程及措施方面。国内的研究前沿主要集中在通过多媒体互联网平台来提高恢复期、偏瘫患者的自理能力等方面;国外的研究前沿集中在从源头切断病因更好地提高脑卒中患者的生存质量,减轻预后护理负担,但是对于脑卒中延续护理平台的选择依旧是趋于传统。结论脑卒中延续护理领域正处在快速发展阶段,我国应进一步加大研究力度,拓宽合作领域,聚焦相关研究热点及前沿,从国外研究中汲取精华,以更好地促进我国脑卒中延续护理的发展。
文摘目的探讨BirthRite坐位分娩组件接产的安全性和有效性,为缩短初产妇第二产程时间,提升产妇生产体验提供参考。方法将住院分娩的低危初产妇236例,第二产程按照产妇意愿分为观察组(116例)和对照组(120例)。观察组采用BirthRite坐位分娩组件坐式分娩,对照组采用电动产床坐式分娩。观察两组产妇第二产程时间、非枕前位占比、阴道助产、产后出血、肩难产、会阴侧切、会阴裂伤、会阴水肿、会阴疼痛程度和产后满意度;比较两组新生儿出生体质量和1 min、5 min Apgar评分。结果对照组中途2例退出。两组均顺利分娩,观察组第二产程时间显著短于对照组,分娩非枕前位占比、肩难产发生率、会阴侧切率、会阴水肿及疼痛程度显著低于对照组,产妇满意度显著高于对照组(均P<0.05)。两组新生儿出生后1 min和5 min Apgar评分均为10分。结论使用BirthRite分娩椅能够缩短第二产程时间,提高产妇满意度,优化坐位分娩结局。
