Two reagents including salicylhydroxamic acid(SHA) and tributyl phosphate(TBP) were tested as collectors either separately or together for electro-flotation of fine cassiterite(<10 μm).Subsequently,the flotation m...Two reagents including salicylhydroxamic acid(SHA) and tributyl phosphate(TBP) were tested as collectors either separately or together for electro-flotation of fine cassiterite(<10 μm).Subsequently,the flotation mechanism of the fine cassiterite was investigated by adsorbance determination,electrophoretic mobility measurements and Fourier transform infra-red(FT-IR) spectrum checking.Results of the flotation experiments show that with SHA as a collector,the collecting performance is remarkably impacted by the pulp pH value as the floatability of cassiterite varies sharply when the pH changes,and flotation with SHA gives distinct maximum at about pH 6.5.Additionally,the floatability of cassiterite is determined by using SHA and TBP as collectors.The range of pulp pH for good floatability is broadened in the presence of TBP as auxiliary collector,and the utilization of TBP improves the recovery of cassiterite modestly.Moreover,the optimum pH value for cassiterite flotation is associated with adsorbance.The results of FT-IR spectrum and the electrophoretic mobility measurements indicate that the adsorption interaction between the collectors and the cassiterite is dominantly a kind of chemical bonding in the form of one or two cycle chelate rings due to the coordination of carbonyl group,hydroxamate and P=O group to the metal tin atoms,where the oxygen atoms contained in carbonyl group,hydroxamate and P=O group of the polar groups have the stereo conditions to form five-membered rings.In addition,the adsorption interactions of SHA and TBP on the surfaces of cassiterite are also dominated by means of hydrogen bonds.展开更多
为探究假单胞菌溶解难溶性无机磷的作用机理,以高效溶磷假单胞菌1616X1为对象,以Ca_(3)(PO_(4))_(2)为磷源,高效液相色谱法测定菌株溶磷过程中的有机酸种类及含量变化,通过Illumina HiSeq X Ten测序平台对假单胞菌1616X1全基因组进行测...为探究假单胞菌溶解难溶性无机磷的作用机理,以高效溶磷假单胞菌1616X1为对象,以Ca_(3)(PO_(4))_(2)为磷源,高效液相色谱法测定菌株溶磷过程中的有机酸种类及含量变化,通过Illumina HiSeq X Ten测序平台对假单胞菌1616X1全基因组进行测序,序列拼装后完成基因预测、功能注释和溶磷相关基因分析。结果表明,假单胞菌1616X1在溶磷过程中产生葡萄糖酸、酒石酸与柠檬酸等多种有机酸,其中,以葡萄糖酸为主,在溶磷量最高时葡萄糖酸含量达17582.06 mg/L;全基因组测序及分析结果表明,菌株1616X1基因组长度5957620 bp,GC含量61.55%,含有5535个编码基因。经COG、GO和KEGG数据库比对和注释,其中,含有吡咯喹啉醌生物合成基因簇(pqq)、磷酸盐转运系统合成基因(pst与pit)、磷饥饿反应调控基因(phoB、phoR与phoH)以及葡萄糖脱氢酶基因(gdh)、吡咯喹啉醌依赖性葡萄糖脱氢酶基因(gcd)、柠檬酸合酶基因(gltA)与酒石酸脱氢酶基因(ttuC)等有机酸合成基因。研究为假单胞菌1616X1溶磷作用规律的阐释和基因改造等后期研究开发奠定基础。展开更多
基金Project(50774094) supported by the National Natural Science Foundation of ChinaProject(2010CB630905) supported by the National Basic Research Program of China
文摘Two reagents including salicylhydroxamic acid(SHA) and tributyl phosphate(TBP) were tested as collectors either separately or together for electro-flotation of fine cassiterite(<10 μm).Subsequently,the flotation mechanism of the fine cassiterite was investigated by adsorbance determination,electrophoretic mobility measurements and Fourier transform infra-red(FT-IR) spectrum checking.Results of the flotation experiments show that with SHA as a collector,the collecting performance is remarkably impacted by the pulp pH value as the floatability of cassiterite varies sharply when the pH changes,and flotation with SHA gives distinct maximum at about pH 6.5.Additionally,the floatability of cassiterite is determined by using SHA and TBP as collectors.The range of pulp pH for good floatability is broadened in the presence of TBP as auxiliary collector,and the utilization of TBP improves the recovery of cassiterite modestly.Moreover,the optimum pH value for cassiterite flotation is associated with adsorbance.The results of FT-IR spectrum and the electrophoretic mobility measurements indicate that the adsorption interaction between the collectors and the cassiterite is dominantly a kind of chemical bonding in the form of one or two cycle chelate rings due to the coordination of carbonyl group,hydroxamate and P=O group to the metal tin atoms,where the oxygen atoms contained in carbonyl group,hydroxamate and P=O group of the polar groups have the stereo conditions to form five-membered rings.In addition,the adsorption interactions of SHA and TBP on the surfaces of cassiterite are also dominated by means of hydrogen bonds.
