Objective.To study expression and localiza tion of Smad4protein,the common-mediator Smad,which is one of intracellular signaling molecules of transforming growth factor-afamily,in rat t estis during postnatal developm...Objective.To study expression and localiza tion of Smad4protein,the common-mediator Smad,which is one of intracellular signaling molecules of transforming growth factor-afamily,in rat t estis during postnatal development.Methods.In this study,whole testes were co llected from S.D rats aged3days,7days,14days and28days,and adult respe ctively.We examined the cellular localization and developmental change of Smad 4in rat testis by immunohistochemical ABC method with glucose oxidase-DAB-nic kel enhancement technique;the quantitative analysis of the immunostaining by t he image analysis system;the Smads pro-teins coexistence in the adult rat test is by the double immune staining for CD14-Smad4and Smad2-Smad4;the prote in expression of Smad during rat testicular development by means of Western blo ts.Results.The protein of Smad4was present in rats from3days of age to adul thood,and the im-munolocalization was exclusively localized to the cytoplasm o f Leydig cells with negative nuclei in the in-terstitial tissue at any time po int.No expression was detected in germ cells.The result of image and sta-tis tical analysis showed that generally,there was a tendency that the expression o f Smad4in the testes increased gradually with the rats developing maturation.C onclusion.Our data provide direct evidence for the molecular mechanism of TGF-aaction in rat testes during postnatal development and spermatogenesis of ra ts.展开更多
Objective: To investigate the expression regulation of thyrotrophin-releasing hormone (TRH) and its receptor (TRH-R) in rat testis and their function in the reproduction and development. Methods: Total RNA of fat test...Objective: To investigate the expression regulation of thyrotrophin-releasing hormone (TRH) and its receptor (TRH-R) in rat testis and their function in the reproduction and development. Methods: Total RNA of fat testicular tissues was used as template. The oligonucleotide primers were designed from the sequence of rat hypothalamus prepro(pp)TRH and pituitary TRH-R cDNA for reverse transcription- polymerase chain reaction (RT- PCR ). Specific fragments of ppTRH and TRH-R cDNA were cloned and sequenced. Results: DNA sequence analysis indicated that cDNA Sequences of ppTRH and TRH-R from rat testis were consistent with those of hypothalamus ppTRH and pituitary TRH-R cDNA. Conclusion: TRH may function as a new autocrine or paracrine regulator of testicular function via a transmembrane receptor very similar or identical to that in pituitary.展开更多
【目的】分析谷胱甘肽过氧化物酶-5(glutathione peroxidase-5,GPx5)在正常及隐睾症双峰驼睾丸及附睾中的分布与表达,探索其在双峰驼隐睾时精子的抗氧化对生殖的调控作用。【方法】选取成年(5岁)双峰驼正常睾丸附睾10对及隐睾睾丸附睾6...【目的】分析谷胱甘肽过氧化物酶-5(glutathione peroxidase-5,GPx5)在正常及隐睾症双峰驼睾丸及附睾中的分布与表达,探索其在双峰驼隐睾时精子的抗氧化对生殖的调控作用。【方法】选取成年(5岁)双峰驼正常睾丸附睾10对及隐睾睾丸附睾6对,利用HE染色、胶原染色、网状染色观察其组织学结构特征,通过免疫组织化学染色、Western blotting、免疫荧光染色结合Image Pro Plus 13.0图像分析研究GPx5的表达及分布。【结果】与正常组相比,隐睾组双峰驼生精小管发育不良且管腔直径极显著缩小(P<0.01),间质细胞减少,附睾管腔缩小且上皮形成空泡,睾丸及附睾间质网状纤维和胶原纤维较丰富。免疫组织化学结果显示,GPx5在正常组双峰驼睾丸中未见明显表达,在隐睾组支持细胞中可见明显表达。Western blotting结果显示,与正常组相比,GPx5在隐睾组双峰驼睾丸和附睾体中表达量极显著升高(P<0.01),在附睾头中表达量极显著降低(P<0.01),在附睾尾中表达量无显著差异(P>0.05)。免疫荧光定位显示,与正常组相比,GPx5在隐睾组双峰驼睾丸和附睾尾中表达量极显著升高(P<0.01),在附睾头中表达量极显著降低(P<0.01),在附睾体中表达量无显著差异(P>0.05)。【结论】双峰驼隐睾的睾丸及附睾趋向纤维化;GPx5蛋白表达量在双峰驼隐睾睾丸中显著增加,且在支持细胞中表达增强,生精小管中的氧化应激影响了精子的正常生成;在附睾头的主细胞表达微弱,提示附睾微环境的抗氧化异常,精子损伤明显。展开更多
This study investigated the impact of simulated microgravity on acute injury induced by low doses of carbon ions in male reproductive organs of mice,and determined alterations in spermatogenic function and expression ...This study investigated the impact of simulated microgravity on acute injury induced by low doses of carbon ions in male reproductive organs of mice,and determined alterations in spermatogenic function and expression levels of apoptotic factors in mice following exposure to acute irradiation after 7 days of simulated microgravity.The results demonstrated that significant reductions in spermatozoa,primary spermatocytes and spermatogonia,and increased globular cells in seminiferous tubule and pro-apoptotic proteins were observed in the group exposed to over0.4 Gy irradiation.Collectively,the data suggest that lesions inflicted by simulated microgravity are not markedly modified by lower doses of irradiation(0.2 Gy)in mouse testis compared to the control group.However,testicular impairments were markedly evident in the group exposed to higher doses of carbon ions plus simulated microgravity,which may be due at least in part to elevated apoptosis initiated by the mitochondrial apoptosis pathway in germ cells.展开更多
The number and quality of sperm are decreased due to bisphenol A(BPA) exposure, an endocrine-disrupting chemical on the male reproductive system, especially in advanced paternal age(APA). Procyanidin C-1(PCY-1), an an...The number and quality of sperm are decreased due to bisphenol A(BPA) exposure, an endocrine-disrupting chemical on the male reproductive system, especially in advanced paternal age(APA). Procyanidin C-1(PCY-1), an antioxidant from grape seed(Vitis vinifera L.), has demonstrated anti-viral, anti-melanogenic and immunostimulatory effects. Therefore, this study aims to determine the effects of PCY-1 intervention on sperm parameters, testis morphological changes, serum testosterone, oestradiol, and luteinizing hormone(LH) concentrations and the expression of apoptotic(Bax and Bcl-2) and mitochondria-related(Mfn1 and Opa1) genes in BPA-exposed aged mice. Results revealed that PCY-1 intervention improves aged male fertility in BPA-exposed conditions by decreasing abnormal sperms percentage and increasing spermatogenic cell diameter and epithelial height. PCY-1 also decreased oestradiol, and increased LH and testosterone levels. The gene expression of Bax was significantly down-regulated by PCY-1 intervention. In contrast, Bcl-2 was substantially up-regulated. Expression of Mfn1 and Opa1 genes were also significantly up-regulated in the PCY intervention group. Hence, it is demonstrated that PCY-1 was able to mitigate the adverse effects of BPA on reproductive parameters of aged mice. Collectively, we postulated that PCY-1 has a potential role in protecting the ageing male reproductive system against the damaging impacts of BPA.展开更多
文摘Objective.To study expression and localiza tion of Smad4protein,the common-mediator Smad,which is one of intracellular signaling molecules of transforming growth factor-afamily,in rat t estis during postnatal development.Methods.In this study,whole testes were co llected from S.D rats aged3days,7days,14days and28days,and adult respe ctively.We examined the cellular localization and developmental change of Smad 4in rat testis by immunohistochemical ABC method with glucose oxidase-DAB-nic kel enhancement technique;the quantitative analysis of the immunostaining by t he image analysis system;the Smads pro-teins coexistence in the adult rat test is by the double immune staining for CD14-Smad4and Smad2-Smad4;the prote in expression of Smad during rat testicular development by means of Western blo ts.Results.The protein of Smad4was present in rats from3days of age to adul thood,and the im-munolocalization was exclusively localized to the cytoplasm o f Leydig cells with negative nuclei in the in-terstitial tissue at any time po int.No expression was detected in germ cells.The result of image and sta-tis tical analysis showed that generally,there was a tendency that the expression o f Smad4in the testes increased gradually with the rats developing maturation.C onclusion.Our data provide direct evidence for the molecular mechanism of TGF-aaction in rat testes during postnatal development and spermatogenesis of ra ts.
基金Supported by National Nature Science Foundation of China, No. 39870109
文摘Objective: To investigate the expression regulation of thyrotrophin-releasing hormone (TRH) and its receptor (TRH-R) in rat testis and their function in the reproduction and development. Methods: Total RNA of fat testicular tissues was used as template. The oligonucleotide primers were designed from the sequence of rat hypothalamus prepro(pp)TRH and pituitary TRH-R cDNA for reverse transcription- polymerase chain reaction (RT- PCR ). Specific fragments of ppTRH and TRH-R cDNA were cloned and sequenced. Results: DNA sequence analysis indicated that cDNA Sequences of ppTRH and TRH-R from rat testis were consistent with those of hypothalamus ppTRH and pituitary TRH-R cDNA. Conclusion: TRH may function as a new autocrine or paracrine regulator of testicular function via a transmembrane receptor very similar or identical to that in pituitary.
文摘【目的】分析谷胱甘肽过氧化物酶-5(glutathione peroxidase-5,GPx5)在正常及隐睾症双峰驼睾丸及附睾中的分布与表达,探索其在双峰驼隐睾时精子的抗氧化对生殖的调控作用。【方法】选取成年(5岁)双峰驼正常睾丸附睾10对及隐睾睾丸附睾6对,利用HE染色、胶原染色、网状染色观察其组织学结构特征,通过免疫组织化学染色、Western blotting、免疫荧光染色结合Image Pro Plus 13.0图像分析研究GPx5的表达及分布。【结果】与正常组相比,隐睾组双峰驼生精小管发育不良且管腔直径极显著缩小(P<0.01),间质细胞减少,附睾管腔缩小且上皮形成空泡,睾丸及附睾间质网状纤维和胶原纤维较丰富。免疫组织化学结果显示,GPx5在正常组双峰驼睾丸中未见明显表达,在隐睾组支持细胞中可见明显表达。Western blotting结果显示,与正常组相比,GPx5在隐睾组双峰驼睾丸和附睾体中表达量极显著升高(P<0.01),在附睾头中表达量极显著降低(P<0.01),在附睾尾中表达量无显著差异(P>0.05)。免疫荧光定位显示,与正常组相比,GPx5在隐睾组双峰驼睾丸和附睾尾中表达量极显著升高(P<0.01),在附睾头中表达量极显著降低(P<0.01),在附睾体中表达量无显著差异(P>0.05)。【结论】双峰驼隐睾的睾丸及附睾趋向纤维化;GPx5蛋白表达量在双峰驼隐睾睾丸中显著增加,且在支持细胞中表达增强,生精小管中的氧化应激影响了精子的正常生成;在附睾头的主细胞表达微弱,提示附睾微环境的抗氧化异常,精子损伤明显。
基金Supported by grants from the National Basic Research Program of China(No.2010CB834202)Knowledge Innovation Projection of Chinese Academy of Science(No.KJCX2-YW-L08)The National Natural Science Foundation of China(No.10835011,No.10675151,No.41161058,No.31060065 and No.10805064)
文摘This study investigated the impact of simulated microgravity on acute injury induced by low doses of carbon ions in male reproductive organs of mice,and determined alterations in spermatogenic function and expression levels of apoptotic factors in mice following exposure to acute irradiation after 7 days of simulated microgravity.The results demonstrated that significant reductions in spermatozoa,primary spermatocytes and spermatogonia,and increased globular cells in seminiferous tubule and pro-apoptotic proteins were observed in the group exposed to over0.4 Gy irradiation.Collectively,the data suggest that lesions inflicted by simulated microgravity are not markedly modified by lower doses of irradiation(0.2 Gy)in mouse testis compared to the control group.However,testicular impairments were markedly evident in the group exposed to higher doses of carbon ions plus simulated microgravity,which may be due at least in part to elevated apoptosis initiated by the mitochondrial apoptosis pathway in germ cells.
基金supported financially by Universiti Teknologi MARA grants (600-IRMI/DANA KCM 5/3/LESTARI – 224/2017), (600-IRMI/My RA 5/3/GIP-030/2017)Ministry of Higher Education (MOHE) grant (600-IRMI/RAGS 5/3 - 72/2015)。
文摘The number and quality of sperm are decreased due to bisphenol A(BPA) exposure, an endocrine-disrupting chemical on the male reproductive system, especially in advanced paternal age(APA). Procyanidin C-1(PCY-1), an antioxidant from grape seed(Vitis vinifera L.), has demonstrated anti-viral, anti-melanogenic and immunostimulatory effects. Therefore, this study aims to determine the effects of PCY-1 intervention on sperm parameters, testis morphological changes, serum testosterone, oestradiol, and luteinizing hormone(LH) concentrations and the expression of apoptotic(Bax and Bcl-2) and mitochondria-related(Mfn1 and Opa1) genes in BPA-exposed aged mice. Results revealed that PCY-1 intervention improves aged male fertility in BPA-exposed conditions by decreasing abnormal sperms percentage and increasing spermatogenic cell diameter and epithelial height. PCY-1 also decreased oestradiol, and increased LH and testosterone levels. The gene expression of Bax was significantly down-regulated by PCY-1 intervention. In contrast, Bcl-2 was substantially up-regulated. Expression of Mfn1 and Opa1 genes were also significantly up-regulated in the PCY intervention group. Hence, it is demonstrated that PCY-1 was able to mitigate the adverse effects of BPA on reproductive parameters of aged mice. Collectively, we postulated that PCY-1 has a potential role in protecting the ageing male reproductive system against the damaging impacts of BPA.
