期刊文献+
共找到177篇文章
< 1 2 9 >
每页显示 20 50 100
Low-complexity PTS scheme based on phase factor sequences optimization 被引量:2
1
作者 JI Ce ZHANG Chao ZHU Wenjing 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2018年第4期707-713,共7页
In this paper, a new partial transmit sequence(PTS)scheme with low computational complexity is proposed for the problems of high computational complexity in the conventional PTS method. By analyzing the relationship... In this paper, a new partial transmit sequence(PTS)scheme with low computational complexity is proposed for the problems of high computational complexity in the conventional PTS method. By analyzing the relationship of candidate sequences in the PTS method under the interleaved partition method, it has been discovered that some candidate sequences generated by phase factor sequences have the same peak average power ratio(PAPR). Hence, phase factor sequences can be optimized to reduce their searching times. Then, the computational process of generating candidate sequences can be simplified by improving the utilization of data and minimizing the calculations of complex multiplication. The performance analysis shows that, compared with the conventional PTS scheme, the proposed approach significantly decreases the computational complexity and has no loss of PAPR performance. 展开更多
关键词 partial transmit sequence (PTS) peak average powerratio (PAPR) phase factor sequences optimization orthogonal frequency division multiplexing (OFDM) low computational com-plexity
在线阅读 下载PDF
Pseudo-Random Sequences Generator Based on Discrete Hyperchaotic Systems 被引量:1
2
作者 Li Changgang & Han Zhengzhi Department of Automation, Shanghai Jiaotong University, Shanghai 200030, P. R. China 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2003年第4期84-89,共6页
We first design a discrete hyperchaotic system via piece-wise linear state feedback. The states of the closed loop system are locally expanding in two directions but absolutely bounded on the whole, which implies hype... We first design a discrete hyperchaotic system via piece-wise linear state feedback. The states of the closed loop system are locally expanding in two directions but absolutely bounded on the whole, which implies hyperchaos. Then, we use three suchlike hyperchaotic systems with different feedback gain matrices to design a pseudo-random sequence generator (PRSG). Through a threshold function, three sub-sequences generated from the output of piecewise linear functions are changed into 0-1 sequences. Then, followed by XOR operation, an unpredictable pseudo-random sequence (PRS) is ultimately obtained. The analysis and simulation results indicate that the PRS, generated with hyperchaotic systems, has desirable statistical features. 展开更多
关键词 discrete hyperchaotic system piecewise linear state feedback pseudo-random sequences.
在线阅读 下载PDF
DEPOSITIONAL SEQUENCES AROUND J/K BOUNDARY IN THE QOMOLONGMA AREA, SOUTH TIBET
3
作者 Shi Xiaoying 1, Nicol Morton 2(1 China University of Geosciences, Beijing 100083,China 2.University of London, London WC1E 7HX,UK) 《地学前缘》 EI CAS CSCD 2000年第S1期21-22,共2页
The uppermost Jurassic to Lower Cretaceous in the Qomolongma (Mt. Everest) area were deposited in a strong subsiding setting on passive continental margin. From Tithonian to Valanginian, the area experienced an enviro... The uppermost Jurassic to Lower Cretaceous in the Qomolongma (Mt. Everest) area were deposited in a strong subsiding setting on passive continental margin. From Tithonian to Valanginian, the area experienced an environment shift from circum\|shelf margin carbonate platform to foreslope, then to deep continental slope [1] . Correspondingly, the sediments change in vertical succession from quartz sandstone, via carbonate grainstone to dark shale intercalated with lithic sandstone of gravity flow. From the Tithonian to Valanginian, eight 3rd\|order sequences have been recognized [1] with clearly delineated sequence boundaries and reasonable biostratigraphic controls of ammonites. From the angle of sequence stratigraphy and sedimentary cycles, the important change happened at ca. 138Ma [2] of the Lower Tithonian (about 120m above Aulacosphinctoides hybonotum zone), where a waved subaerial erosion surface occurs in massive bioclastic grainstone. The boundary at ca. 134 5Ma (latest Tithonian, about 60m below the FAD of \%Berriasella cf. jacobi [3] ) comes to the next. The third one is at ca 132Ma (some 170m above the FAD of Berriasella cf. jacobi [3] , but about 60m below the FAD of Berriasella cf. grandis [3] ). In terms of sea\|level change, the sequence boundary at 134 5Ma is probably the most distinctive one, above which some 60m gravel\|bearing massive medium\|grained quartz sandstone was laid down and obviously truncates the underlying strata. In the dark shale below the sandstone, abundant in ammonites, such as Virgatosphinctes, Aulacosphinctes, Haplophylloceras and Gymnosphinctes are recorded [3] . The quartz sandstone itself does not bear identifiable fossils. Above the quartz sandstone, the strata mainly consists of dark silty shale up to 180m thick, with an prominent marine transgression at the base. Just above the first flooding surface (FFS), quite a number of ammonites such as \%Berriasella\% cf. \%jacobi, Blanofordiceras, Hymalayaites\% and \%Pseudosubplanites \%are documented [3] . The third sequence, which is delineated by submarine truncation of slope fan complexes at bottom, is also characterized by dark shale and silts, yielding ammonites \%Berriasella \%cf. \%grandis\% and B. cf. \%berthei.\%>From the viewpoint of sequence stratigraphy, we herein suggest to take the FFS in the second sequence as the J/K boundary. The sequence boundary below it can be used as a nice auxiliary marker, which represents a large sea\|level fall, and can be correlated widely in the world. From the angle of paleontology, the FFS is also ideal for the boundary, where the most important faunal turnover happened. Theoretically, taking FFS as a chronostratigraphic boundary has more advantages than using sequence boundary. 展开更多
关键词 J/K BOUNDARY DEPOSITIONAL sequences SOUTH TIBET
在线阅读 下载PDF
Optimal training sequences for MIMO systems under correlated fading
4
作者 Pang Jiyong Li Jiandong Lu Zhuo Zhao Linjing Chen Liang 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2008年第1期33-38,共6页
The optimal design of training sequences for channel estimation in multiple-input multiple-output (MIMO) systems under spatially correlated fading is considered. The channel is assumed to be a block-fading model wit... The optimal design of training sequences for channel estimation in multiple-input multiple-output (MIMO) systems under spatially correlated fading is considered. The channel is assumed to be a block-fading model with spatial correlation known at both the transmitter and the receiver. To minimize the channel estimation error, optimal training sequences are designed to exploit full information of the spatial correlation under the criterion of minimum mean square error (MMSE). It is investigated that the spatial correlation is helpful to decrease the estimation error and the proposed training sequences have good performance via simulations. 展开更多
关键词 MIMO channel estimation training sequences spatial correlation minimum mean square error
在线阅读 下载PDF
Dim Moving Small Target Detection by Local and Global Variance Filtering on Temporal Profiles in Infrared Sequences
5
作者 Chen Hao Liu Delian 《航空兵器》 CSCD 北大核心 2019年第6期43-49,共7页
In this paper, the temporal different characteristics between the target and background pixels are used to detect dim moving targets in the slow-evolving complex background. A local and global variance filter on tempo... In this paper, the temporal different characteristics between the target and background pixels are used to detect dim moving targets in the slow-evolving complex background. A local and global variance filter on temporal profiles is presented that addresses the temporal characteristics of the target and background pixels to eliminate the large variation of background temporal profiles. Firstly, the temporal behaviors of different types of image pixels of practical infrared scenes are analyzed.Then, the new local and global variance filter is proposed. The baseline of the fluctuation level of background temporal profiles is obtained by using the local and global variance filter. The height of the target pulse signal is extracted by subtracting the baseline from the original temporal profiles. Finally, a new target detection criterion is designed. The proposed method is applied to detect dim and small targets in practical infrared sequence images. The experimental results show that the proposed algorithm has good detection performance for dim moving small targets in the complex background. 展开更多
关键词 small target detection infrared image sequences complex background temporal profile variance filtering
在线阅读 下载PDF
Global Annotation of Small RNA and MicroRNA Mature Sequences from Developing Ovules of Gossypium hirsutum L.
6
作者 ABDURAKHMONOV Ibrokhim Y DEVOR Eric J BURIEV Zabardast T MAKAMOV Abdusalom SHERMATOV Shukhrat E ABDUKARIMOV Abdusattor 《棉花学报》 CSCD 北大核心 2008年第S1期11-,共1页
The involvement of small RNAs in cotton fiber development is under explored.The objective of this work was to directly clone,annotate,and analyze small RNAs of developing ovules to reveal
关键词 Global Annotation of Small RNA and MicroRNA Mature sequences from Developing Ovules of Gossypium hirsutum L RNA
在线阅读 下载PDF
Almost perfect sequences based on cyclic difference sets 被引量:6
7
作者 Chen Gang Zhao Zhengyu 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2007年第1期155-159,共5页
The perfect sequences are so ideal that all out-of-phase autocorrelation coefficients are zero, and if the sequences are used as the coding modulating signal for the phase-modulated radar, there will be no interferenc... The perfect sequences are so ideal that all out-of-phase autocorrelation coefficients are zero, and if the sequences are used as the coding modulating signal for the phase-modulated radar, there will be no interference of side lobes theoretically. However, it has been proved that there are no binary perfect sequences of period 4 〈 n ≤ 12100. Hence, the almost perfect sequences with all out-of-phase autocorrelation coefficients as zero except one are of great practice in engineering. Currently, the cyclic difference set is one of most effective tools to analyze the binary sequences with perfect periodic autocorrelation function. In this article, two characteristic formulas corresponding to the autocorrelation and symmetric structure of almost perfect sequences are calculated respectively. All almost perfect sequences with period n, which is a multiple of 4, can be figured out by the two formulas. Several almost perfect sequences with different periods have been hunted by the program based on the two formulas and then applied to the simulation program and practical application for ionospheric sounding. 展开更多
关键词 Perfect sequence Almost perfect sequence Cyclic difference sets Autocorrelation coefficients Side lobes Ionospheric sounding
在线阅读 下载PDF
Linear complexity and autocorrelation of a new class of binary generalized cyclotomic sequences of order two and length pqr 被引量:1
8
作者 JIA Wenjuan GAO Juntao ZHANG Peng 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2019年第4期651-661,共11页
Cyclotomic sequences have good cryptographic properties and are closely related to difference sets.This paper proposes a new class of binary generalized cyclotomic sequences of order two and length pqr.Its linear comp... Cyclotomic sequences have good cryptographic properties and are closely related to difference sets.This paper proposes a new class of binary generalized cyclotomic sequences of order two and length pqr.Its linear complexity,minimal polynomial,and autocorrelation are investigated.The results show that these sequences have a large linear complexity when 2∈D1,which means they can resist the Berlekamp-Massey attack.Furthermore,the autocorrelation values are close to 0 with a probability of approximately 1?1/r.Therefore,when r is a big prime,the new sequence has a good autocorrelation. 展开更多
关键词 GENERALIZED cyclotomic sequence linear complexity MINIMAL POLYNOMIAL AUTOCORRELATION value.
在线阅读 下载PDF
基于改进Seq2Seq的船舶轨迹预测模型
9
作者 唐家乐 段兴锋 姚鹏 《上海海事大学学报》 北大核心 2025年第2期18-22,共5页
针对传统循环神经网络(recurrent neural network,RNN)模型收敛速度慢、精度低,导致海上船舶预测轨迹与真实轨迹之间差别较大的问题,构建由RNN组成的Seq2Seq(sequence to sequence)模型。引入注意力机制和卷积神经网络(convolutional ne... 针对传统循环神经网络(recurrent neural network,RNN)模型收敛速度慢、精度低,导致海上船舶预测轨迹与真实轨迹之间差别较大的问题,构建由RNN组成的Seq2Seq(sequence to sequence)模型。引入注意力机制和卷积神经网络(convolutional neural network,CNN)对模型进行改进,加强对数据特征的提取能力,加快模型收敛速度并提高轨迹预测精度。实验结果显示:与传统RNN模型相比,Seq2Seq模型的均方误差、均方根误差和平均绝对误差分别降低81.41%、12.67%和62.43%;与Seq2Seq模型相比,改进Seq2Seq模型的均方误差、均方根误差和平均绝对误差分别降低42.87%、69.27%和45.79%。 展开更多
关键词 船舶轨迹预测 Seq2Seq(sequence to sequence) 注意力机制 卷积神经网络(CNN) 循环神经网络(RNN)
在线阅读 下载PDF
Five novel ZNF469 gene mutations in sporadic keratoconus patients in the Han Chinese population
10
作者 CAO Yanna DENG Zhihong +3 位作者 HE Guiyun XIAO Li ZHANG Feng SU Feng 《中南大学学报(医学版)》 北大核心 2025年第6期931-939,共9页
Objective:Keratoconus(KC)is a progressive corneal ectasia disorder,arising from a myriad of causes including genetic predispositions,environmental factors,biomechanical influences,and inflammatory reactions.This study... Objective:Keratoconus(KC)is a progressive corneal ectasia disorder,arising from a myriad of causes including genetic predispositions,environmental factors,biomechanical influences,and inflammatory reactions.This study aims to identify potential pathogenetic gene mutations in patients with sporadic KC in the Han Chinese population.Methods:Twenty-five patients with primary KC as well as 50 unrelated population matched healthy controls,were included in this study to identify potential pathogenic gene mutations among sporadic KC patients in the Han Chinese population.Sanger sequencing and whole-exome sequencing(WES)were used to analyze mutations in the zinc finger protein 469(ZNF469)gene.Bioinformatics analysis was conducted to explore the potential role of ZNF469 in KC pathogenesis.Results:Five novel heterozygous missense variants were identified in KC patients.Among them,2 compound heterozygous variants,c.8986G>C(p.E2996Q)with c.11765A>C(p.D3922A),and c.4423C>G(p.L1475V)with c.10633G>A(p.G3545R),were determined to be possible pathogenic factors for KC.Conclusion:Mutations in the ZNF469 gene may contribute to the development of KC in the Han Chinese population.These mutation sites may provide valuable information for future genetic screening of KC patients and their families. 展开更多
关键词 KERATOCONUS ZNF469 gene MUTATION Sanger sequencing Han Chinese population
在线阅读 下载PDF
Regulatory effects of seed soaking with a beneficial microbial agent on the rhizosphere soil microflora of edible lilies
11
作者 MAN Hua-li LI Hui +3 位作者 SHI Gui-hong YANG Hong-yu LI Mou-qiang SHI Gui-ying 《植物营养与肥料学报》 北大核心 2025年第5期994-1005,共12页
【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact ... 【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact of this soaking method on the fungal and bacterial community structures using next-generation sequencing technology(NGS).【Methods】Lily bulbs were soaked in a seed treating agent containing beneficial microbes(SP treatment)for 4 hours.Subsequently,they were planted in soil in July and sampled in September to assess plant growth,rhizosphere soil physicochemical properties,and microorganism community structures.In addition,we employed the software PICRUSt and FUNGuild to predict bacterial pathways and fungal functions.【Results】Under SP treatment,there were significant alterations in fungi and bacteria community structures,accompanied by improved soil nutrient status.Notably,the relative abundance of dominant microorganism groups,such as the fungi Basidiomycota,Pseudeurotium,Cladophialophora,Microascus,and Dactylonectria,as well as the bacteria Proteobacteria,Chloroflexi,Ochrobactrium,Lysobacter,and RB41,underwent notable changes.Microorganism function prediction results indicated a reduction in pathotrophic fungi(including plant pathogens)and an increase in endophytic and saprotrophic fungi under SP treatment.Among the top 20 metabolism pathways,80%were upregulated in SP treatment compared to the CK.【Conclusions】Seed soaking with beneficial microbial strain promotes the growth of Lanzhou lily bulbs.The beneficial microorganisms play a crucial role in regulating soil microbial structures,enhancing the accumulation of endophytic fungi,reducing the abundance of pathogens,and improving soil functions.Furthermore,specific microbial groups are found to be involved in maintaining soil health. 展开更多
关键词 edible lily second-generation sequencing technology(NGS) seed treating fungal community structure bacterial community structure PROPHYLAXIS
在线阅读 下载PDF
拟穴青蟹(CA)_n微卫星DNA多态性引物筛选 被引量:2
12
作者 宋忠魁 聂振平 王芳宇 《海洋科学》 CAS CSCD 北大核心 2013年第10期98-103,共6页
利用FIASCO(Fast Isolation by AFLP Sequences Containing repeats)技术建立拟穴青蟹Scylla paramamosain基因组文库,并与生物素标记的(CA)15寡核苷酸探针杂交,联合磁珠富集法构建拟穴青蟹微卫星富集文库。测序194个阳性菌落,分析其中... 利用FIASCO(Fast Isolation by AFLP Sequences Containing repeats)技术建立拟穴青蟹Scylla paramamosain基因组文库,并与生物素标记的(CA)15寡核苷酸探针杂交,联合磁珠富集法构建拟穴青蟹微卫星富集文库。测序194个阳性菌落,分析其中的150条序列,结果表明:两碱基重复类型占90%以上,其中重复拷贝数在30以上的占27.45%;含微卫星座位189个,其中完美型146个、非完美型28个和复合型15个。设计125对引物扩增一个拟穴青蟹野生群体(含20个个体),其中的19对引物能稳定扩增且片段大小基本符合理论长度。遗传变异分析表明,17个位点表现出高度多态性,16个位点显著偏离Hardy-Weinberg平衡(P<0.05),4组两两位点间存在连锁不平衡现象(P<0.0026,经Bonferroni法校正),7个微卫星位点可能存在无效等位基因。若排除混合微卫星位点的引物对以及扩增位点PIC(polymorphism information content)值在0.5以下的引物对,则13对引物能用于拟穴青蟹群体遗传学等研究。 展开更多
关键词 FIASCO(Fast Isolation by AFLP sequences Containing repeats)技术 磁珠富集法 拟穴青蟹Scylla paramamosain 微卫星
在线阅读 下载PDF
Research on the navigation method of high speed differential rotation guided ammunition with ballistic assistance prediction under GNSS denial
13
作者 Ning Liu Kejun Hu +5 位作者 Bin Hu Haorui Li Kai Shen Wenhao Qi Junfang Fan Zhong Su 《Defence Technology(防务技术)》 2025年第7期275-289,共15页
In complex environments such as high dynamics and weak signals,a satellite signal compensation method based on prefabricated trajectory assistance and an improved adaptive Kalman filter is proposed for a 155 mm differ... In complex environments such as high dynamics and weak signals,a satellite signal compensation method based on prefabricated trajectory assistance and an improved adaptive Kalman filter is proposed for a 155 mm differential rotating rear-body control-guided projectile to address the situation of satellite signal flickering and loss in projectile navigation systems due to environmental limitations.First,establish the system state and measurement equation when receiving satellite signals normally.Second,a seven-degree-of-freedom external ballistic model is constructed,and the ideal trajectory output from the ballistic model is used to provide the virtual motion state of the projectile,which is input into a filter as a substitute observation when satellite signals are lost.Finally,an adaptive Kalman filter(AKF)is designed,the proposed adaptive Kalman filter can accurately adjust the estimation error covariance matrix and Kalman gain in real-time based on information covariance mismatch.The simulation results show that compared to the classical Kalman filter,it can reduce the average positioning error by more than 38.21%in the case of short-term and full-range loss of satellite signals,providing a new idea for the integrated navigation of projectiles with incomplete information under the condition of satellite signal loss. 展开更多
关键词 GNSS refusal Ballistic assistance Guided ammunition Adaptive kalman filter Covariance of innovation sequence
在线阅读 下载PDF
基于SSR分子标记的新疆83个常规陆地棉品种亲缘关系分析
14
作者 牛清东 杨延龙 《中国棉花》 2025年第7期32-37,共6页
采用简单重复序列(simple repeated sequence,SSR)分子标记技术对2022―2024年新疆种业公司提交的83份样品(包括早熟品种、早中熟品种)进行亲缘关系分析,以期为棉花品种的管理和选择提供依据。使用农作物SSR数据比对软件(登记号:2025SR0... 采用简单重复序列(simple repeated sequence,SSR)分子标记技术对2022―2024年新疆种业公司提交的83份样品(包括早熟品种、早中熟品种)进行亲缘关系分析,以期为棉花品种的管理和选择提供依据。使用农作物SSR数据比对软件(登记号:2025SR0343132)对样品SSR数据位点进行两两比较,分别利用Powermarker V3.25、NTSYSpc-2.10e、Structure 2.3.4、GenALEx 6.502进行SSR多态性分析、亲缘关系分析、群体结构分析和主成分分析。结果表明,60对SSR引物共扩增出153个等位变异,平均等位变异数为2.55,平均多态性信息含量为0.32。83份品种两两比较共产生了3403个组合,差异位点范围为0~47,位点差异大于2的组合有3290个,占组合数的96.7%。亲缘关系分析、群体结构分析和主坐标分析的分类结果基本一致,83份样品被划分为2个群体pop1和pop2,pop2包含pop2A和pop2B亚群。亲缘关系分析显示,棉种样品间的相似系数在0.503~1.000,平均值为0.690,说明棉种样品间遗传背景较窄、亲缘关系较近。主坐标分析结果显示:pop1共有样品15份,均为早熟品种;pop2A共有样品52份,其中含早熟品种29份、早中熟品种23份;pop2B共有样品16份,其中有早熟品种9份、早中熟品种7份,并没有明显的地理分类特征。这些结果可为新疆棉花品种选育和管理提供参考。 展开更多
关键词 陆地棉 简单重复序列(simple repeated sequence SSR) 分子标记 亲缘关系 近似品种 群体结构分析 主成分分析 多态性
在线阅读 下载PDF
杏ISSR反应体系的优化和指纹图谱的构建 被引量:60
15
作者 刘威生 冯晨静 +3 位作者 杨建民 刘冬成 张爱民 李绍华 《果树学报》 CAS CSCD 北大核心 2005年第6期626-629,共4页
以杏品种清密沙为试材,用引物UBC825〔序列为(AC)8T〕研究了PCR反应体系的主要成分及退火温度对杏ISSR扩增结果的影响。结果表明:Primer、Mg2+、Taq酶浓度对扩增效果有明显影响,而模板DNA和dNTPs含量对扩增结果影响不大。优化的反应体系... 以杏品种清密沙为试材,用引物UBC825〔序列为(AC)8T〕研究了PCR反应体系的主要成分及退火温度对杏ISSR扩增结果的影响。结果表明:Primer、Mg2+、Taq酶浓度对扩增效果有明显影响,而模板DNA和dNTPs含量对扩增结果影响不大。优化的反应体系为:20μL的反应体系中含10ng模板DNA、0.1mmol/LdNTP、0.25μmol/LPrimer、2.5mmol/LMg2+,0.5UTaqPolymerase。适宜退火温度为50-52.1℃。用引物UBC825和UBC868〔序列为(GAA)5〕在优化的反应条件下建立了5个种12份杏材料的ISSR指纹图谱。 展开更多
关键词 ISSR(inter—simple SEQUENCE repeat) 反应体系 退火温度 优化
在线阅读 下载PDF
14份杏种质的ISSR分析 被引量:26
16
作者 冯晨静 张元慧 +4 位作者 徐秀英 师国洪 刘威生 孟庆瑞 杨建民 《河北农业大学学报》 CAS CSCD 北大核心 2005年第5期52-55,62,共5页
以冀光为试材,研究了PCR反应体系的主要成分及退火温度对杏ISSR扩增结果的影响.同时,从42对ISSR引物中筛选出12对扩增条带清晰、多态性好的引物进行扩增,并运用UPGMA聚类分析法,分析了12份杏种质的亲缘关系及多样性.结果表明:在20 μL... 以冀光为试材,研究了PCR反应体系的主要成分及退火温度对杏ISSR扩增结果的影响.同时,从42对ISSR引物中筛选出12对扩增条带清晰、多态性好的引物进行扩增,并运用UPGMA聚类分析法,分析了12份杏种质的亲缘关系及多样性.结果表明:在20 μL的反应体系中,模板DNA含量在10~80 ng均能得到较好的扩增;dNTP用量对扩增无明显影响;而引物、Mg2+的最适浓度分别为0.25 μmol/L、0.25 mmol/L;Taq酶在0.5~4 U均能得到好的扩增条带;退火温度在50~52.1℃范围内均能得到清晰的条带;并在此基础上建立了杏ISSR反应体系.当相似系数在0.444~0.452之间时,将12份杏材料划分为3类:①普通杏(Amentaca vulgaris),西伯利亚杏(A.sibirica),辽杏(A.mandshurica),藏杏(A.holosericea)类型;②仁用杏品种一窝蜂;③紫杏(A. dasycarpa). 展开更多
关键词 ISSR(inter-simple SEQUENCE repeat) 品种鉴定 亲缘关系 反应体系
在线阅读 下载PDF
玉米穗部性状QTL定位 被引量:18
17
作者 吕学高 蔡一林 +4 位作者 陈天青 徐德林 王伟林 刘志斋 王久光 《西南大学学报(自然科学版)》 CAS CSCD 北大核心 2008年第2期64-70,共7页
利用SSR分子标记构建的遗传连锁图谱对玉米的穗长、穗粗、穗行数、行粒数、轴粗、秃尖长、200粒质量和穗粒质量等8个性状进行了基因定位.穗长检测到2个QTLs,分布于Ch1-1、Ch9连锁群;穗粗检测到7个QTLs,分布于Ch1、Ch1-1、Ch2、Ch4、Ch4-... 利用SSR分子标记构建的遗传连锁图谱对玉米的穗长、穗粗、穗行数、行粒数、轴粗、秃尖长、200粒质量和穗粒质量等8个性状进行了基因定位.穗长检测到2个QTLs,分布于Ch1-1、Ch9连锁群;穗粗检测到7个QTLs,分布于Ch1、Ch1-1、Ch2、Ch4、Ch4-1、Ch6、Ch7连锁群;穗行数检测到6个QTLs,分布于Ch1、Ch2、Ch5、Ch6、Ch9、Ch10连锁群;行粒数检测到1个QTL,位于Ch9连锁群;轴粗检测到6个QTLs,分布于Ch1、Ch4、Ch5、Ch6、Ch7、Ch10连锁群;200粒质量检测到2个QTLs,分布于Ch7、Ch8连锁群;穗粒质量检测到3个QTLs,分布于Ch5、Ch6、Ch9连锁群.研究表明,穗部性状QTLs在玉米10条染色体上分布不均匀,呈现成簇分布现象;各个QTL位点上起增、减效作用的等位基因在高值亲本和低值亲本中的分布不均匀. 展开更多
关键词 玉米 穗部性状 Simple SEQUENCE Repeat(SSR) QTL定位
在线阅读 下载PDF
三倍体葡萄柚实生后代多倍体的发掘与SSR遗传鉴定 被引量:16
18
作者 梁武军 解凯东 +3 位作者 谢宗周 徐强 伊华林 郭文武 《果树学报》 CAS CSCD 北大核心 2015年第1期13-18,共6页
【目的】从三倍体葡萄柚自然授粉的实生后代中发掘多倍体,希望获得一批多倍体新种质,并为探索三倍体遗传规律奠定基础。【方法】秋季采摘三倍体葡萄柚成熟果实,剥取种子,MT+1 mg·L-1GA3培养基播种,待种子萌发后,用流式细胞仪快速... 【目的】从三倍体葡萄柚自然授粉的实生后代中发掘多倍体,希望获得一批多倍体新种质,并为探索三倍体遗传规律奠定基础。【方法】秋季采摘三倍体葡萄柚成熟果实,剥取种子,MT+1 mg·L-1GA3培养基播种,待种子萌发后,用流式细胞仪快速检测其倍性,并采用SSR(simple sequence repeat)分子标记鉴定其不同倍性后代植株的来源。【结果】获得了二倍体、三倍体、四倍体、五倍体、六倍体和疑似非整倍体等不同倍性的再生植株各172、52、7、1、1、18株。用13对多态性SSR引物鉴定其来源,结果表明,由二倍体可能三倍体母本所产生的1X配子与周边其他二倍体品种的花粉授粉而来,也可能由三倍体母本产生的重组型2X配子的无融合生殖而来;三倍体大部分由珠心细胞发育而来;四倍体为杂交起源的四倍体;五倍体和六倍体与母本(三倍体葡萄柚)的带型完全相同。【结论】上述活体材料的发掘为柑橘多倍体育种奠定了种质基础。 展开更多
关键词 柑橘 多倍体 SSR(simple sequence repeat)分子标记 三倍体
在线阅读 下载PDF
SSR分子标记在作物遗传育种中的应用 被引量:32
19
作者 李丽 王海岗 +1 位作者 张晓丽 彭锁堂 《山西农业科学》 2008年第3期15-18,共4页
SSR(Simple Sequence Repeat)是建立在PCR技术上的一种广泛应用的分子标记,具有含量丰富、多态性高、共显性等优点。简要介绍了SSR标记技术的发现、原理和特点,并总结了该技术在作物遗传多样性、遗传图谱构建、分子标记辅助选择等方面... SSR(Simple Sequence Repeat)是建立在PCR技术上的一种广泛应用的分子标记,具有含量丰富、多态性高、共显性等优点。简要介绍了SSR标记技术的发现、原理和特点,并总结了该技术在作物遗传多样性、遗传图谱构建、分子标记辅助选择等方面的应用。 展开更多
关键词 SSR(Simple SEQUENCE Repeat) 分子标记 作物 应用
在线阅读 下载PDF
不同落叶果树根际微生物群落代谢与组成的差异性研究 被引量:18
20
作者 邵微 于会丽 +6 位作者 张培基 徐国益 乔宪生 高登涛 王志强 田鹏 司鹏 《果树学报》 CAS CSCD 北大核心 2020年第9期1371-1383,共13页
【目的】从根际微生物群落代谢与组成方面探讨不同落叶果树根际差异性。【方法】利用Biolog ECO板方法与Illumina Miseq sequencing技术,以未种植果树区域土壤为对照,研究苹果、梨及葡萄等8种落叶果树根际微生物群落的代谢与组成。【结... 【目的】从根际微生物群落代谢与组成方面探讨不同落叶果树根际差异性。【方法】利用Biolog ECO板方法与Illumina Miseq sequencing技术,以未种植果树区域土壤为对照,研究苹果、梨及葡萄等8种落叶果树根际微生物群落的代谢与组成。【结果】8种果树根际微生物的代谢活性与功能多样性指数(除Pielou均匀度指数E外)均显著高于对照。比较8种果树根际微生物群落的碳源利用特征情况发现,杏树根际微生物群落对酚酸类物质(PC,phenolic compounds)的利用比率显著下降,对碳水化合物(CH,carbohydrate)的利用比率显著上升,而对照土壤微生物群落对氨基酸类(AA,amino acids)碳源利用比率最高。细菌与真菌群落纲水平相对丰度表明,8种果树根际细菌与真菌群落相对丰度存在明显差异,其中苹果与核桃γ-变形菌纲相对丰度较其他果树显著上升;而与细菌群落相比,8种果树根际真菌群落在纲水平上差异更为明显。基于纲水平优势菌与微生物群落代谢之间的相关性分析表明,微生物群落代谢与Betaproteobacteria、Alphaproteobacteria及Sphingobacteriia呈显著正相关,而与Gemmatimonadetes、Sordariomycetes、Glomeromycetes及Chytridiomycetes均呈显著负相关。另外,RDA分析表明,微生物功能多样性(除E外)与细菌α多样性呈正相关,而与真菌α多样性呈负相关。【结论】8种果树微生物群落碳代谢存在显著差异。8种果树根际细菌与真菌群落均存在显著差异,而真菌群落较细菌差异更为显著。根际微生物群落代谢与细菌群落呈正相关,而与真菌群落呈负相关。 展开更多
关键词 落叶果树 根际土壤微生物群落 Biolog Eco-板 Illumina Miseq sequencing技术 相关分析
在线阅读 下载PDF
上一页 1 2 9 下一页 到第
使用帮助 返回顶部