OBJECTIVE To investigate the transcriptomic details on the biosynthetic pathways in different parts of the Panax notoginseng and the pharmacological activity of the saponins extracted from the flower(FS)on vascular in...OBJECTIVE To investigate the transcriptomic details on the biosynthetic pathways in different parts of the Panax notoginseng and the pharmacological activity of the saponins extracted from the flower(FS)on vascular insufficiency conditions.METHODS RNA sequencing of three different Panax notoginseng tissues was performed using next generation DNA sequencing and differential gene expression was validated by real-time PCR.In order to determine pro-angiogenic and therapeutic effects of FS on myocardial infraction(MI),FS was examined on the endothelial cell migration assay,vascular insufficiency model in zebrafish and MI model in rats.RESULTS After assembling the high quality sequencing reads into 107 340 unigenes,biochemical pathways were predicted and 9 908 unigenes were assigned to 135 KEGG pathways.Among them,270 unigenes were identified to be involved in triterpene saponin biosynthesis as well as 350 and 342unigenes were predicted to encode cytochrome P450 sand glycosyltransferases,respectively.One unigene was annotated as CYP716A53v2,probably participates in the formation of protopanaxatriol from protopanaxadiol and the differential expression of this gene was confirmed by real-time PCR.In addition,the pharmacological evaluation demonstrate that FS significantly promoted vascular endothelial growth factor(VEGF)induced the migration of human umbilical vein endothelial cells(HUVECs)and partially restored defective intersegmental vessels in a chemically induced vascular insufficiency model of zebrafish larva.Moreover,the two week posttreatment of the rat MI model with FS(25-50mg·kg-1·d-1)induced approximately 3-fold upregulation of VEGF mRNA expression,with a concomitant increase in blood vessel density in the peri-infarct area of the heart by 50.7%,compared to 41.4%in the MI group.Furthermore,TUNEL analysis indicates a reduction in the mean apoptotic nuclei per field in peri-infarct myocardium upon FS treatment.CONCLUSION We have established a global transcriptome dataset for Panax notoginseng and provided additional genetic information for further genome-wide research and analyses.Candidate genes involved in ginsenoside biosynthesis,including putative cytochrome P450 sand glycosyltransferases were obtained.The transcriptomes in different plant tissues also provide invaluable resources for future study of the differences in physiological processes and secondary metabolites in different parts of P.notoginseng.And the significant pro-angiogenic effect of FS in multiple experimental models renders the purified saponin preparation as potential preventive and therapeutic agent for cardiovascular diseases yet to be developed.展开更多
OBJECTIVE The strategy and techniques of metabolomics was applied for the pharmacology and molecular mechanism research of Panax notoginseng(PN)in traditional Chinese medicine.METHODS The global metabolic profiles of ...OBJECTIVE The strategy and techniques of metabolomics was applied for the pharmacology and molecular mechanism research of Panax notoginseng(PN)in traditional Chinese medicine.METHODS The global metabolic profiles of PN were investigated by the NMR-based metabolomics.The different parts of PN were scanned into metabolic profiles by 1H-NMR.The significant differences of these metabolic profiles were analyzed by PCA,PLS-DA,PLS-R,etc.The pharmacological effects including free radical scavenging activity(FRSA),anti-proliferation to human colorectal cancer cell line(HCT116),xanthine oxidase inhibition,were followed in vitro.Additionally,the molecular mechanism of xanthine degrading process by PN was attempted by 1H-NMR.RESULTS The NMR-based metabolic profiles of different parts(upper part of root,middle part of root,lower part of root,hairy root,leaf and stem)of PN presented significant differences by multivariate statistical analysis.The hairy root and leaf revealed highest anti-proliferative effect to HCT116;the leaf and stem of PN showed highest level of FRSA;the leaf,stem,hairy root effected the xanthine degrading 1 metabolic pathway.And the H-NMR based molecular mechanism experiment showed that the xanthine metabolic pathway degraded by PN depended on the direct inhibition to xanthine.CONCLUSION The metabolomics strategy provided complementary chemical profiling to medicinal herbs,which accelerated the development of pharmacology and mechanism of action in traditional medicine.The subsidiary parts of PN,as leaf,stem and hairy root,have the potential to develop new drugs in curing cancer,inflammation and gout.展开更多
为保证三七[Panax notoginseng(Burk)F. H. Chen]药材的质量稳定与溯源,简化施肥并提高其种植效率,进行了田间小区肥料替代试验。以习惯施肥为对照(CK),基于聚磷酸铵型水溶肥(SRF)设计替代施肥(T1)、减量施肥(T2)、配方施肥(T3)3个处理...为保证三七[Panax notoginseng(Burk)F. H. Chen]药材的质量稳定与溯源,简化施肥并提高其种植效率,进行了田间小区肥料替代试验。以习惯施肥为对照(CK),基于聚磷酸铵型水溶肥(SRF)设计替代施肥(T1)、减量施肥(T2)、配方施肥(T3)3个处理。结果表明,SRF处理下,三七的农艺性状和氮(N)、磷(P)、钾(K)营养成分,以及土壤中有机质(SOM)、阳离子交换量(CEC)等土壤理化性质与CK相比无显著差异;与CK相比,T3没有改变土壤细菌的物种多样性和丰富度,也未对土壤真菌的物种丰富度产生影响,但T3提高了土壤真菌的物种多样性。相比习惯施肥,聚磷酸铵型水溶肥在实现三七节本、增效种植的同时,还可以通过改善土壤菌群结构降低施肥对土壤环境的危害,可大面积推广应用。展开更多
基金The project supported by the Scheme B funding of the project′Establishment of the Centre for Microbial Genomics and Proteomics′and the Scheme D funding of the project′Enhancing the capabilities and strengthening the research personnel of CUHK in Bioinformatics′of the Focused Investment Scheme of The Chinese University of Hong Kongthe Overseas and Hong Kong,Macao Young Scholars Collaborative Research Fund by the Natural National Science Foundation of China(81328025)+1 种基金Science and Technology Development Fund of Macao SAR(058/2009and 078/2011/A3)Research Committee,University of Macao
文摘OBJECTIVE To investigate the transcriptomic details on the biosynthetic pathways in different parts of the Panax notoginseng and the pharmacological activity of the saponins extracted from the flower(FS)on vascular insufficiency conditions.METHODS RNA sequencing of three different Panax notoginseng tissues was performed using next generation DNA sequencing and differential gene expression was validated by real-time PCR.In order to determine pro-angiogenic and therapeutic effects of FS on myocardial infraction(MI),FS was examined on the endothelial cell migration assay,vascular insufficiency model in zebrafish and MI model in rats.RESULTS After assembling the high quality sequencing reads into 107 340 unigenes,biochemical pathways were predicted and 9 908 unigenes were assigned to 135 KEGG pathways.Among them,270 unigenes were identified to be involved in triterpene saponin biosynthesis as well as 350 and 342unigenes were predicted to encode cytochrome P450 sand glycosyltransferases,respectively.One unigene was annotated as CYP716A53v2,probably participates in the formation of protopanaxatriol from protopanaxadiol and the differential expression of this gene was confirmed by real-time PCR.In addition,the pharmacological evaluation demonstrate that FS significantly promoted vascular endothelial growth factor(VEGF)induced the migration of human umbilical vein endothelial cells(HUVECs)and partially restored defective intersegmental vessels in a chemically induced vascular insufficiency model of zebrafish larva.Moreover,the two week posttreatment of the rat MI model with FS(25-50mg·kg-1·d-1)induced approximately 3-fold upregulation of VEGF mRNA expression,with a concomitant increase in blood vessel density in the peri-infarct area of the heart by 50.7%,compared to 41.4%in the MI group.Furthermore,TUNEL analysis indicates a reduction in the mean apoptotic nuclei per field in peri-infarct myocardium upon FS treatment.CONCLUSION We have established a global transcriptome dataset for Panax notoginseng and provided additional genetic information for further genome-wide research and analyses.Candidate genes involved in ginsenoside biosynthesis,including putative cytochrome P450 sand glycosyltransferases were obtained.The transcriptomes in different plant tissues also provide invaluable resources for future study of the differences in physiological processes and secondary metabolites in different parts of P.notoginseng.And the significant pro-angiogenic effect of FS in multiple experimental models renders the purified saponin preparation as potential preventive and therapeutic agent for cardiovascular diseases yet to be developed.
基金The project supported by National Research Foundation of Korea funded by the Korean government(MIST)(NRF-2011-0021055)
文摘OBJECTIVE The strategy and techniques of metabolomics was applied for the pharmacology and molecular mechanism research of Panax notoginseng(PN)in traditional Chinese medicine.METHODS The global metabolic profiles of PN were investigated by the NMR-based metabolomics.The different parts of PN were scanned into metabolic profiles by 1H-NMR.The significant differences of these metabolic profiles were analyzed by PCA,PLS-DA,PLS-R,etc.The pharmacological effects including free radical scavenging activity(FRSA),anti-proliferation to human colorectal cancer cell line(HCT116),xanthine oxidase inhibition,were followed in vitro.Additionally,the molecular mechanism of xanthine degrading process by PN was attempted by 1H-NMR.RESULTS The NMR-based metabolic profiles of different parts(upper part of root,middle part of root,lower part of root,hairy root,leaf and stem)of PN presented significant differences by multivariate statistical analysis.The hairy root and leaf revealed highest anti-proliferative effect to HCT116;the leaf and stem of PN showed highest level of FRSA;the leaf,stem,hairy root effected the xanthine degrading 1 metabolic pathway.And the H-NMR based molecular mechanism experiment showed that the xanthine metabolic pathway degraded by PN depended on the direct inhibition to xanthine.CONCLUSION The metabolomics strategy provided complementary chemical profiling to medicinal herbs,which accelerated the development of pharmacology and mechanism of action in traditional medicine.The subsidiary parts of PN,as leaf,stem and hairy root,have the potential to develop new drugs in curing cancer,inflammation and gout.
文摘为保证三七[Panax notoginseng(Burk)F. H. Chen]药材的质量稳定与溯源,简化施肥并提高其种植效率,进行了田间小区肥料替代试验。以习惯施肥为对照(CK),基于聚磷酸铵型水溶肥(SRF)设计替代施肥(T1)、减量施肥(T2)、配方施肥(T3)3个处理。结果表明,SRF处理下,三七的农艺性状和氮(N)、磷(P)、钾(K)营养成分,以及土壤中有机质(SOM)、阳离子交换量(CEC)等土壤理化性质与CK相比无显著差异;与CK相比,T3没有改变土壤细菌的物种多样性和丰富度,也未对土壤真菌的物种丰富度产生影响,但T3提高了土壤真菌的物种多样性。相比习惯施肥,聚磷酸铵型水溶肥在实现三七节本、增效种植的同时,还可以通过改善土壤菌群结构降低施肥对土壤环境的危害,可大面积推广应用。
