Carcass,meat quality,and feather-down traits of 123 geese from an F_1 population of Wanxi White goose(male parent)and Sichuan White goose(female parent)were measured,and an additional 12 compound indices were construc...Carcass,meat quality,and feather-down traits of 123 geese from an F_1 population of Wanxi White goose(male parent)and Sichuan White goose(female parent)were measured,and an additional 12 compound indices were constructed from the single traits,using factor analysis.The coding region of melanocortin receptor-4(MC4R)gene was amplified by PCR and the products were digested with four restriction endonucleases Hinf I,Ear I,Pst I,and Pvu II to detect restriction fragment length polymorphism(RFLP)in the MC4R gene,to analyze its associations with the traits.The only RFLP was exposed by digesting with Hinf I indicating two genotypes,HH and Hh,with frequencies of allele H and h being 0.8902 and 0.1098 in the F_1 population.Significant effects of genotype HH were found on carcass weight,eviscerated weight(with giblet),liver weight,heart weight,stomach percentage,abdominal fat percentage,breast muscle drip water loss and down proportion and many of the compound indices,and all genotypic contribution percentages(CP,genotypic effect on the population mean)were between-5%and 5%;effects of genotype Hh with the MC4R gene mutation,were not significant.Effects of genotype Hh were significant on two of the compound indices related to fat deposition:the fat index(CP=3.314%,P<0.05)and fatty acid quality(CP=1.218%,P<0.05).展开更多
[目的]探讨犬黑素皮质素受体-4(MC4R)基因与肥胖的关系。[方法]通过PCR-RFLP技术分析犬MC4R基因多态性与体重的关系。通过载体构建和细胞培养技术来构建犬MC4R基因的真核表达载体并转染MDCK细胞,研究该基因的体外表达情况。最后通过小...[目的]探讨犬黑素皮质素受体-4(MC4R)基因与肥胖的关系。[方法]通过PCR-RFLP技术分析犬MC4R基因多态性与体重的关系。通过载体构建和细胞培养技术来构建犬MC4R基因的真核表达载体并转染MDCK细胞,研究该基因的体外表达情况。最后通过小鼠尾静脉高压注射重组真核表达载体的方法进行动物试验。[结果]比格犬226 bp C/A多态性与体重呈显著相关。犬MC4R基因在MDCK细胞内成功表达。MC4R重组体裸质粒注射后小鼠的体重有明显变化。[结论]MC4R基因能够促进小鼠的体重增加。该基因与肥胖的发生密切相关,将为肥胖机制的研究提供理论依据。展开更多
基金supported by a loth 5-year personwith-ability prject of Anhui province,Natural Science Foundation of Anhui Educational Institution(No.2006KJ/69B)Key Scientific Project of Anhui Provincial Department of Science and Technology(No.07020303055,08010302070)
文摘Carcass,meat quality,and feather-down traits of 123 geese from an F_1 population of Wanxi White goose(male parent)and Sichuan White goose(female parent)were measured,and an additional 12 compound indices were constructed from the single traits,using factor analysis.The coding region of melanocortin receptor-4(MC4R)gene was amplified by PCR and the products were digested with four restriction endonucleases Hinf I,Ear I,Pst I,and Pvu II to detect restriction fragment length polymorphism(RFLP)in the MC4R gene,to analyze its associations with the traits.The only RFLP was exposed by digesting with Hinf I indicating two genotypes,HH and Hh,with frequencies of allele H and h being 0.8902 and 0.1098 in the F_1 population.Significant effects of genotype HH were found on carcass weight,eviscerated weight(with giblet),liver weight,heart weight,stomach percentage,abdominal fat percentage,breast muscle drip water loss and down proportion and many of the compound indices,and all genotypic contribution percentages(CP,genotypic effect on the population mean)were between-5%and 5%;effects of genotype Hh with the MC4R gene mutation,were not significant.Effects of genotype Hh were significant on two of the compound indices related to fat deposition:the fat index(CP=3.314%,P<0.05)and fatty acid quality(CP=1.218%,P<0.05).
文摘[目的]探讨犬黑素皮质素受体-4(MC4R)基因与肥胖的关系。[方法]通过PCR-RFLP技术分析犬MC4R基因多态性与体重的关系。通过载体构建和细胞培养技术来构建犬MC4R基因的真核表达载体并转染MDCK细胞,研究该基因的体外表达情况。最后通过小鼠尾静脉高压注射重组真核表达载体的方法进行动物试验。[结果]比格犬226 bp C/A多态性与体重呈显著相关。犬MC4R基因在MDCK细胞内成功表达。MC4R重组体裸质粒注射后小鼠的体重有明显变化。[结论]MC4R基因能够促进小鼠的体重增加。该基因与肥胖的发生密切相关,将为肥胖机制的研究提供理论依据。
文摘黑素皮质素受体-4(melanocortin-4 receptor,MC4R)是下丘脑腹内侧核分泌的一类肽类物质,为黑素皮质素受体家族5个亚型(MC1-5R)之一。在哺乳动物中,MC4R具有介导瘦蛋白(leptin)的功能,是一个调节能量平衡与能量动态平衡的重要信号分子,可与其内源性配体黑素皮质素激素(melanocortin,MC)或刺鼠色蛋白(agouti protein,agouti蛋白)和agouti相关蛋白(agouti related protein,AGRP)相结合,从而在控制食欲和体重稳态中起关键作用。因此,MC4R在人类肥胖研究中作为重要的调节因子倍受关注。最近有研究表明:MC4R的1232位点的G→A的突变与绵羊背膘厚度间存在关联,AG和AA型较GG型具有较高的背膘厚度。
