A new reaction system to determine nonlinear chemical fingerprint(NCF)and its use in identification method based on double reaction system was researched.Panax ginsengs,such as ginseng,American ginseng and notoginseng...A new reaction system to determine nonlinear chemical fingerprint(NCF)and its use in identification method based on double reaction system was researched.Panax ginsengs,such as ginseng,American ginseng and notoginseng were identified by the method.The NCFs of the three samples of Panax ginsengs were determined through two nonlinear chemical systems,namely system 1 consisting of sample components,H2SO4,MnSO4,NaBrO3,acetone and the new system,system 2 consisting of sample components,H2SO4,(NH4)4Ce(SO4)2,NaBrO3 and citric acid.The comparison between the results determined through systems 1 and 2 shows that the speed to determine NCF through system 2 is much faster than that through system 1;for systems 1 and 2,the system similarities of the same kind of samples are≥98.09%and 99.78%,respectively,while those of different kinds of samples are≤63.04%and 86.34%,respectively.The results to identify the kinds of some samples by system similarity pattern show that both the accuracies of identification methods based on single system 1 and 2 are≥95.6%,and the average values are 97.1%and 96.3%,respectively;the accuracy of the method based on double system is≥97.8%,and the average accuracy is 99.3%.The accuracy of the method based on double system is higher than that based on any single system.展开更多
A cell suspension culture of Panax ginseng which may be continuously subcultured has been established. Embryogenic callus derived from clutured young leaves was used to initiate the culture.Plant growth regulators, ba...A cell suspension culture of Panax ginseng which may be continuously subcultured has been established. Embryogenic callus derived from clutured young leaves was used to initiate the culture.Plant growth regulators, basal medium formula and carbohydrate levels were examined to determine their various effects on suspension culture cell growth and development. The best selection of plant growth regulator, hasal medium and carbohydrate level is 2 mg / L 2,4-D: 0.5 mg / L KT,MS and 3% sucrose respectively.展开更多
To screen genetic polymorphisms of Panax ginseng, as well as those of Panax quinquefolium and Panax notoginseng, analysis of random amplified polymorphic DNA （RAPD） was performed using 120 random primers. Of the suc...To screen genetic polymorphisms of Panax ginseng, as well as those of Panax quinquefolium and Panax notoginseng, analysis of random amplified polymorphic DNA （RAPD） was performed using 120 random primers. Of the successful amplicons obtained, the Panax ginseng-specific RAPD marker C-12 was cloned into a TA vector and sequenced （Genl3ank access number KU553472）. Based on the sequence analysis results, a pair of primers specific to C-12 was designed. Finally, a SCAR marker-based identification system for Panax ginseng was developed after optimization of the reaction conditions. Using this method, two positive bands were stably observed at 300 bp and 130 bp in 33 batches of Panax ginseng samples tested, while negative results were obtained for another 101 batches of samples, including Panax quinquefolium, Panax notoginseng, adulterants, and other medicinal herbs. Thus, we successfully developed a PCR-based method for rapid and effective identification of Panax ginseng, which can be effectively used for the protection and utilization of germplasm resources and identification of the origins of Panax ginseng samples.展开更多
OBJECTIVE Panax ginseng C.A.Meyer(ginseng)is a well-known medicinal plant worldwide and a key ingredient in many commercially-available health products.It is used as a tonic for invigoration and for tification in time...OBJECTIVE Panax ginseng C.A.Meyer(ginseng)is a well-known medicinal plant worldwide and a key ingredient in many commercially-available health products.It is used as a tonic for invigoration and for tification in times of fatigue and debility or declining capacity for work and concentration.Previous in-house study has surveyed over three hundred ginseng and ginseng products(including P.ginseng,P.quinquefolius,P.notoginseng,P.pseudoginseng)available in Singapore.This review presents an overview of the pharmacological activities and herb-drug interactions of P.ginseng and its ginsenosides.METHODS Literature searches of PubMed and ScienceDirect were done to identify pharmacological activities and herb-drug interactions of P.ginseng,its extracts and its chemical components,including ginsenosides.Studies of whole plant extracts include both White ginseng and Red ginseng.The studies for the pharmacological activities of whole plant extract were limited to those published from 2009 to 2015.There was no restriction on the time frame of other studies.Terms such as″P.ginseng″,″Ginsenosides″were searched.Studies found included in vitro assays,in vivo animal studies,human clinical trials as well as individual case reports.RESULTS A total of 112 studies were found on whole plant extracts and 257 studies on its individual components.Whole plant extracts of ginseng were found to possess over fifty different pharmacological activities,while its individual components exhibit parts of this spectrum.P.ginseng was found to interact with drugs such as 5-fluorouracil,irinotecan,mitomycin C,docetaxel,cisplatin,alcohol,midazolam,warfarin,phenelzine,raltegravir and imatinib.CONCLUSION P.ginseng and its components exhibit a wide range of pharmacological activities and interact with some drugs.There remain much opportunities for future research.展开更多
Fibrosis is a phenomenon of fibrous connective tissue increase in the organs. Activated hepatic stellate cells （HSCs） are the preferred targets of anti-hepatic fibrotic therapies. Ginseng and Platycodon grandiflorum...Fibrosis is a phenomenon of fibrous connective tissue increase in the organs. Activated hepatic stellate cells （HSCs） are the preferred targets of anti-hepatic fibrotic therapies. Ginseng and Platycodon grandiflorum are impor- tant traditional Chinese medicine herbs. The study was to investigate the anti-fibrosis effects of ginseng extract and Platycodon grandiflorum extract in HSCs. Extract include Ginseng water extract and alcohol extract, Platycodon gran- diflorum water extract and alcohol extract, combination of both water extract and alcohol extract. Activated hepatic stellate cells were treated with Ginseng and Platycodon grandiflorum extracts for 24h. The maximum concentration of the drug is 2000 mg · L^-1, the lowest concentration of 7.8 mg · L^-1 , with concentration gradient dilution. 3-（4 , 5- dimethylthiazol-2-yl）-2, 5-diphenylte-trazolium bromide （MTT） can evaluate the survival rate of cells. The expres- sions of SIRT1, NF-KB and p53 were detected by western blot. The results showed that extract effectively decreased the HSC cells viability in concentration dependent manner. Among them, the effect of combination of both extract is best. Ginseng and Platycodon grandiflorum activated the protein expressions of SIRT1 in activated HSCs. The expres- sion of NF-KB and p53 were significantly decreased by Ginseng and Platycodon grandiflorum. This study demonstrated that ginseng extract and Platycodon grandiflorum extract showed obviously anti-fibrosis effect, and decreased expres-sion of NF-KB and p53 via SIRT1 signaling pathway.展开更多
基金Project(61533021)supported by the National Natural Science Foundation of ChinaProject(R201706)supported by Hunan Food Pharmaceutical,China
文摘A new reaction system to determine nonlinear chemical fingerprint(NCF)and its use in identification method based on double reaction system was researched.Panax ginsengs,such as ginseng,American ginseng and notoginseng were identified by the method.The NCFs of the three samples of Panax ginsengs were determined through two nonlinear chemical systems,namely system 1 consisting of sample components,H2SO4,MnSO4,NaBrO3,acetone and the new system,system 2 consisting of sample components,H2SO4,(NH4)4Ce(SO4)2,NaBrO3 and citric acid.The comparison between the results determined through systems 1 and 2 shows that the speed to determine NCF through system 2 is much faster than that through system 1;for systems 1 and 2,the system similarities of the same kind of samples are≥98.09%and 99.78%,respectively,while those of different kinds of samples are≤63.04%and 86.34%,respectively.The results to identify the kinds of some samples by system similarity pattern show that both the accuracies of identification methods based on single system 1 and 2 are≥95.6%,and the average values are 97.1%and 96.3%,respectively;the accuracy of the method based on double system is≥97.8%,and the average accuracy is 99.3%.The accuracy of the method based on double system is higher than that based on any single system.
文摘A cell suspension culture of Panax ginseng which may be continuously subcultured has been established. Embryogenic callus derived from clutured young leaves was used to initiate the culture.Plant growth regulators, basal medium formula and carbohydrate levels were examined to determine their various effects on suspension culture cell growth and development. The best selection of plant growth regulator, hasal medium and carbohydrate level is 2 mg / L 2,4-D: 0.5 mg / L KT,MS and 3% sucrose respectively.
基金Project(2014ZX09304307-002)supported by the Major Program of Science and Technology Foundation of ChinaProject supported by Technology Platform for Quality/Safety Inspection and Risk Management of Traditional Chinese Medicine,China+1 种基金Project(2014SK2001)supported by the Key Program Foundation of Hunan Provincial Science&Technology Department,ChinaProject(XSYK-R201502)supported by the Hunan Provincial Food and Drug Administration under Key Project of Science and Technology for Food and Drug Safety,China
文摘To screen genetic polymorphisms of Panax ginseng, as well as those of Panax quinquefolium and Panax notoginseng, analysis of random amplified polymorphic DNA （RAPD） was performed using 120 random primers. Of the successful amplicons obtained, the Panax ginseng-specific RAPD marker C-12 was cloned into a TA vector and sequenced （Genl3ank access number KU553472）. Based on the sequence analysis results, a pair of primers specific to C-12 was designed. Finally, a SCAR marker-based identification system for Panax ginseng was developed after optimization of the reaction conditions. Using this method, two positive bands were stably observed at 300 bp and 130 bp in 33 batches of Panax ginseng samples tested, while negative results were obtained for another 101 batches of samples, including Panax quinquefolium, Panax notoginseng, adulterants, and other medicinal herbs. Thus, we successfully developed a PCR-based method for rapid and effective identification of Panax ginseng, which can be effectively used for the protection and utilization of germplasm resources and identification of the origins of Panax ginseng samples.
基金The project supported by Lee Foundation Grant(TCH and KHL,R-184-000-200-720)and research scholarship(WHN)
文摘OBJECTIVE Panax ginseng C.A.Meyer(ginseng)is a well-known medicinal plant worldwide and a key ingredient in many commercially-available health products.It is used as a tonic for invigoration and for tification in times of fatigue and debility or declining capacity for work and concentration.Previous in-house study has surveyed over three hundred ginseng and ginseng products(including P.ginseng,P.quinquefolius,P.notoginseng,P.pseudoginseng)available in Singapore.This review presents an overview of the pharmacological activities and herb-drug interactions of P.ginseng and its ginsenosides.METHODS Literature searches of PubMed and ScienceDirect were done to identify pharmacological activities and herb-drug interactions of P.ginseng,its extracts and its chemical components,including ginsenosides.Studies of whole plant extracts include both White ginseng and Red ginseng.The studies for the pharmacological activities of whole plant extract were limited to those published from 2009 to 2015.There was no restriction on the time frame of other studies.Terms such as″P.ginseng″,″Ginsenosides″were searched.Studies found included in vitro assays,in vivo animal studies,human clinical trials as well as individual case reports.RESULTS A total of 112 studies were found on whole plant extracts and 257 studies on its individual components.Whole plant extracts of ginseng were found to possess over fifty different pharmacological activities,while its individual components exhibit parts of this spectrum.P.ginseng was found to interact with drugs such as 5-fluorouracil,irinotecan,mitomycin C,docetaxel,cisplatin,alcohol,midazolam,warfarin,phenelzine,raltegravir and imatinib.CONCLUSION P.ginseng and its components exhibit a wide range of pharmacological activities and interact with some drugs.There remain much opportunities for future research.
文摘Fibrosis is a phenomenon of fibrous connective tissue increase in the organs. Activated hepatic stellate cells （HSCs） are the preferred targets of anti-hepatic fibrotic therapies. Ginseng and Platycodon grandiflorum are impor- tant traditional Chinese medicine herbs. The study was to investigate the anti-fibrosis effects of ginseng extract and Platycodon grandiflorum extract in HSCs. Extract include Ginseng water extract and alcohol extract, Platycodon gran- diflorum water extract and alcohol extract, combination of both water extract and alcohol extract. Activated hepatic stellate cells were treated with Ginseng and Platycodon grandiflorum extracts for 24h. The maximum concentration of the drug is 2000 mg · L^-1, the lowest concentration of 7.8 mg · L^-1 , with concentration gradient dilution. 3-（4 , 5- dimethylthiazol-2-yl）-2, 5-diphenylte-trazolium bromide （MTT） can evaluate the survival rate of cells. The expres- sions of SIRT1, NF-KB and p53 were detected by western blot. The results showed that extract effectively decreased the HSC cells viability in concentration dependent manner. Among them, the effect of combination of both extract is best. Ginseng and Platycodon grandiflorum activated the protein expressions of SIRT1 in activated HSCs. The expres- sion of NF-KB and p53 were significantly decreased by Ginseng and Platycodon grandiflorum. This study demonstrated that ginseng extract and Platycodon grandiflorum extract showed obviously anti-fibrosis effect, and decreased expres-sion of NF-KB and p53 via SIRT1 signaling pathway.
