Aim To investigate whether Kv7 channels opener retigabine could alleviate memory impairment induced by chronic restraint stress （CRS） and the underlying neuroprotective mechanisms. Methods Adult male Kunming （KM） ...Aim To investigate whether Kv7 channels opener retigabine could alleviate memory impairment induced by chronic restraint stress （CRS） and the underlying neuroprotective mechanisms. Methods Adult male Kunming （KM） mice, weighing 20 - 25 g, were restrained in well-ventilated Plexiglass tubes for 6 h daily beginning from 10 ： 00 to 16 ： 00 for 21 consecutive days. Mice were injected with retigabine （ 10 mg · kg^-1） or vehicle （ 10% DM- SO） 30 rain before restraint stress for 21 days. After stressor cessation, the spatial learning and memory was deter- mined by Morris water maze test, the levels of p-Akt, p-GSK-3β and p-Erkl/2 of hippocampal tissues were exam- ined by western blot. Results Compared with control group, CRS mice exhibited significantly longer escape laten- cies on day 2, 3 and 4 （P 〈 0.05, P 〈 0. 01, P 〈 0.01 ） respectively, but retigabine （ 10 mg · kg^-1） treatment had no influences on escape latencies compared with CRS group. During the probe test, CRS mice spent significant less time in target quadrant than control group （P 〈 0.01 ）. Compared with CRS group, retigabine （ 10 mg · kg^-1 ） treatment increased the time spent in target quadrant （P 〈 0.01 ）. Additionally, the swimming speed showed no significant differences among groups. Western blot results showed that the levels of p-Akt, p-GSK-3β and p-Erkl/ 2 in the hippocampus of CRS mice were significantly decreased compared with control group. Compared with CRS group, retigabine （ 10 mg· kg^-1） treatment strongly prevented the reduction of p-Akt and p-GSK-3 β （P 〈 0.01 ）, but had no effect on the reduction of p-Erkl/2. Conclusion Retigabine protected against CRS-induced spatial memory retrieval impairment partly via activation of Akt/GSK-3β signaling pathway.展开更多
为优化安全带固定式儿童乘员约束系统(CRS)安全带设计,用"试验设计方法(DOE)",优化约束路径参数。用CRS有限元模型,建立Hybrid III 3岁儿童假人正面碰撞仿真模型,用台车试验结果进行了验证。改进了CRS骨架结构,用正交试验设计...为优化安全带固定式儿童乘员约束系统(CRS)安全带设计,用"试验设计方法(DOE)",优化约束路径参数。用CRS有限元模型,建立Hybrid III 3岁儿童假人正面碰撞仿真模型,用台车试验结果进行了验证。改进了CRS骨架结构,用正交试验设计,研究安全带导向环固定位置、骨架座椅厚度及儿童乘员与五点式安全带之间的摩擦因数等因素,对头部伤害指标(HIC15)值、胸部合成加速度、胸部垂直加速度等损伤参数的影响。结果表明:头部前倾位移量,从高于法规限值(550 mm)的1.5%,降到低于限值的15%。安全带导向环固定位置是最为敏感的因素;因此,优化导向环固定位置及降低摩擦因数,可减少头部最大位移量。展开更多
目的：评价不合作患儿在全身麻醉下或束缚下进行牙科治疗后,其牙科畏惧水平及就诊行为的变化。方法：全身麻醉组为全身麻醉下进行牙科治疗的4~6岁重度牙科畏惧症患儿31例,束缚组为在性别、年龄、治疗前焦虑水平、口腔内龋坏情况与全身...目的：评价不合作患儿在全身麻醉下或束缚下进行牙科治疗后,其牙科畏惧水平及就诊行为的变化。方法：全身麻醉组为全身麻醉下进行牙科治疗的4~6岁重度牙科畏惧症患儿31例,束缚组为在性别、年龄、治疗前焦虑水平、口腔内龋坏情况与全身麻醉组严格匹配的在束缚下进行牙科治疗的患儿31例。应用MCDASf（face version of the Modified Child Dental Anxiety Scale,MCDASf）中文版量表评价两组患儿治疗前、治疗后和2~3周复诊时主观畏惧水平的变化,同时评价两组患儿治疗时和复诊时的临床就诊行为变化。结果：全身麻醉组治疗后、复诊前MCDASf中文版量表平均得分较治疗前下降,差异均有统计学意义。束缚组治疗后、复诊前MCDASf中文版量表平均得分均较治疗前略上升,但差异无统计学意义。两组间MCDASf中文版量表治疗前、后变化的差异有统计学意义。全身麻醉组患儿经全身麻醉治疗后复诊时的临床就诊行为表现较治疗时的临床就诊行为表现有所改善,两者差异有统计学意义（P〈0.01）。束缚组治疗后复诊时的临床行为表现较束缚治疗时的临床就诊行为有所改善,差异有统计学意义（P〈0.01）。结论：全身麻醉下牙科治疗能够缓解牙科畏惧症患儿就诊中的焦虑、恐惧情绪,提高患儿治疗的合作性,改善其短期复诊的就诊行为。束缚下牙科治疗后患儿的牙科畏惧水平略有增高,但差异无统计学意义,短期复诊时的就诊行为表现有所改善。展开更多
文摘Aim To investigate whether Kv7 channels opener retigabine could alleviate memory impairment induced by chronic restraint stress （CRS） and the underlying neuroprotective mechanisms. Methods Adult male Kunming （KM） mice, weighing 20 - 25 g, were restrained in well-ventilated Plexiglass tubes for 6 h daily beginning from 10 ： 00 to 16 ： 00 for 21 consecutive days. Mice were injected with retigabine （ 10 mg · kg^-1） or vehicle （ 10% DM- SO） 30 rain before restraint stress for 21 days. After stressor cessation, the spatial learning and memory was deter- mined by Morris water maze test, the levels of p-Akt, p-GSK-3β and p-Erkl/2 of hippocampal tissues were exam- ined by western blot. Results Compared with control group, CRS mice exhibited significantly longer escape laten- cies on day 2, 3 and 4 （P 〈 0.05, P 〈 0. 01, P 〈 0.01 ） respectively, but retigabine （ 10 mg · kg^-1） treatment had no influences on escape latencies compared with CRS group. During the probe test, CRS mice spent significant less time in target quadrant than control group （P 〈 0.01 ）. Compared with CRS group, retigabine （ 10 mg · kg^-1 ） treatment increased the time spent in target quadrant （P 〈 0.01 ）. Additionally, the swimming speed showed no significant differences among groups. Western blot results showed that the levels of p-Akt, p-GSK-3β and p-Erkl/ 2 in the hippocampus of CRS mice were significantly decreased compared with control group. Compared with CRS group, retigabine （ 10 mg· kg^-1） treatment strongly prevented the reduction of p-Akt and p-GSK-3 β （P 〈 0.01 ）, but had no effect on the reduction of p-Erkl/2. Conclusion Retigabine protected against CRS-induced spatial memory retrieval impairment partly via activation of Akt/GSK-3β signaling pathway.
文摘为优化安全带固定式儿童乘员约束系统(CRS)安全带设计,用"试验设计方法(DOE)",优化约束路径参数。用CRS有限元模型,建立Hybrid III 3岁儿童假人正面碰撞仿真模型,用台车试验结果进行了验证。改进了CRS骨架结构,用正交试验设计,研究安全带导向环固定位置、骨架座椅厚度及儿童乘员与五点式安全带之间的摩擦因数等因素,对头部伤害指标(HIC15)值、胸部合成加速度、胸部垂直加速度等损伤参数的影响。结果表明:头部前倾位移量,从高于法规限值(550 mm)的1.5%,降到低于限值的15%。安全带导向环固定位置是最为敏感的因素;因此,优化导向环固定位置及降低摩擦因数,可减少头部最大位移量。
文摘目的：评价不合作患儿在全身麻醉下或束缚下进行牙科治疗后,其牙科畏惧水平及就诊行为的变化。方法：全身麻醉组为全身麻醉下进行牙科治疗的4~6岁重度牙科畏惧症患儿31例,束缚组为在性别、年龄、治疗前焦虑水平、口腔内龋坏情况与全身麻醉组严格匹配的在束缚下进行牙科治疗的患儿31例。应用MCDASf（face version of the Modified Child Dental Anxiety Scale,MCDASf）中文版量表评价两组患儿治疗前、治疗后和2~3周复诊时主观畏惧水平的变化,同时评价两组患儿治疗时和复诊时的临床就诊行为变化。结果：全身麻醉组治疗后、复诊前MCDASf中文版量表平均得分较治疗前下降,差异均有统计学意义。束缚组治疗后、复诊前MCDASf中文版量表平均得分均较治疗前略上升,但差异无统计学意义。两组间MCDASf中文版量表治疗前、后变化的差异有统计学意义。全身麻醉组患儿经全身麻醉治疗后复诊时的临床就诊行为表现较治疗时的临床就诊行为表现有所改善,两者差异有统计学意义（P〈0.01）。束缚组治疗后复诊时的临床行为表现较束缚治疗时的临床就诊行为有所改善,差异有统计学意义（P〈0.01）。结论：全身麻醉下牙科治疗能够缓解牙科畏惧症患儿就诊中的焦虑、恐惧情绪,提高患儿治疗的合作性,改善其短期复诊的就诊行为。束缚下牙科治疗后患儿的牙科畏惧水平略有增高,但差异无统计学意义,短期复诊时的就诊行为表现有所改善。
