The development of transgenic cotton varieties resistant to bollworms has been a major success of applying plant genetic engineering technology to agriculture,evidenced by phenomenal increase in
A novel rice gene OsAPT2,which encodes a putative adenine phosphoribosyl transferase(APRT),was cloned.Its full-length cDNA is 1125bp,composing an ORF encoding 212 amino acid residues and a stop cordon,a 5' UTR of ...A novel rice gene OsAPT2,which encodes a putative adenine phosphoribosyl transferase(APRT),was cloned.Its full-length cDNA is 1125bp,composing an ORF encoding 212 amino acid residues and a stop cordon,a 5' UTR of 123 bp and a 3' UTR of 363 bp.The sequence data have been submitted to the DDBJ/EMBL/GenBank databases(accession number:AY238894).The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously reported APRTs.The genomic OsAPT2 gene contains 7 exons and 6 introns.Its total length is 4758 bp.Then,an antisense expression vector of the full-length OsAPT2 cDNA was constructed and transformed into rice variety Taibei309 by Agrobacterium tumefaciens mediated transformation method.In total,650 T0 transgenic plants were obtained based on both antibiotic screening and specific PCR identification.One hundred individuals of them were selected and planted in Hainan Island.From those 11 male sterile lines with seed-setting rate lower than 3% in bagged spike were obtained.Results suggest that OsAPT2 is involved in male sterility.Nine of the 11 male sterile lines were constitutive sterile lines;two of the 11 male sterile lines were thermo-sensitive genic male sterile lines,which may be useful in hybride rice breeding.展开更多
Long-term fertility cycle,low self-compatibility and short flowering period limited the tea breeding by conventional method.It is necessary to apply biotechnology,especially genetic transformation method,to obtain new...Long-term fertility cycle,low self-compatibility and short flowering period limited the tea breeding by conventional method.It is necessary to apply biotechnology,especially genetic transformation method,to obtain new mutants and to accelerate tea breeding.This paper reviews the status and progress in genetic transformation and related influencing factors.It has been noticed that tea genetic transformation has advantages in getting useful target mutants.The genetic transformation by Agrobacterium-mediated and gene gun methods are usually used.However,further research should be focused on improvement of plant regeneration.展开更多
An orthogonal wavelet transform fractionally spaced blind equalization algorithm based on the optimization of genetic algorithm(WTFSE-GA) is proposed in viewof the lowconvergence rate,large steady-state mean square er...An orthogonal wavelet transform fractionally spaced blind equalization algorithm based on the optimization of genetic algorithm(WTFSE-GA) is proposed in viewof the lowconvergence rate,large steady-state mean square error and local convergence of traditional constant modulus blind equalization algorithm(CMA).The proposed algorithm can reduce the signal autocorrelation through the orthogonal wavelet transform of input signal of fractionally spaced blind equalizer,and decrease the possibility of CMA local convergence by using the global random search characteristics of genetic algorithm to optimize the equalizer weight vector.The proposed algorithm has the faster convergence rate and smaller mean square error compared with FSE and WT-FSE.The efficiency of the proposed algorithm is proved by computer simulation of underwater acoustic channels.展开更多
To preserve the original signal as much as possible and filter random noises as many as possible in image processing,a threshold optimization-based adaptive template filtering algorithm was proposed.Unlike conventiona...To preserve the original signal as much as possible and filter random noises as many as possible in image processing,a threshold optimization-based adaptive template filtering algorithm was proposed.Unlike conventional filters whose template shapes and coefficients were fixed,multi-templates were defined and the right template for each pixel could be matched adaptively based on local image characteristics in the proposed method.The superiority of this method was verified by former results concerning the matching experiment of actual image with the comparison of conventional filtering methods.The adaptive search ability of immune genetic algorithm with the elitist selection and elitist crossover(IGAE) was used to optimize threshold t of the transformation function,and then combined with wavelet transformation to estimate noise variance.Multi-experiments were performed to test the validity of IGAE.The results show that the filtered result of t obtained by IGAE is superior to that of t obtained by other methods,IGAE has a faster convergence speed and a higher computational efficiency compared with the canonical genetic algorithm with the elitism and the immune algorithm with the information entropy and elitism by multi-experiments.展开更多
Since the advent of transgenic technology,the incorporation of gene(s)encoding traits of economic importance in cotton is being practiced worldwide.However,factors like recalcitrant nature of cotton cultivars,in vitro...Since the advent of transgenic technology,the incorporation of gene(s)encoding traits of economic importance in cotton is being practiced worldwide.However,factors like recalcitrant nature of cotton cultivars,in vitro regeneration via tissue culture(especially via somatic embryogenesis),genotype dependency,long and toilsome protocols impede the pace of development of transgenic cotton.Besides that,types and age of explants,media composition,plant growth regulators and other environmental factors affect in vitro cotton regeneration significantly.The studies of genetic control of in vitro regeneration in plants have elucidated the role of certain transcription factor genes that are induced and expressed during somatic embryogenesis.Among these transcription factors,BABY BOOM(BBM)plays a very important role in signal transduction pathway,leading to cell differentiation and somatic embryos formation.The role of BBM has been established in plant cell proliferation,growth and development even without exogenous growth regulators.This review intends to provide an informative summary of regeneration and transformation problems in cotton and the latest developments in utilization of BBM transcription factors in cotton.We believe that the use of BBM will not only ease cotton genetic improvement but will also accelerate cotton breeding programmes.展开更多
In this paper, several factors that affect the efficiency of in vitro adventitious bud regeneration and Agrobacterium tumefaciens-mediated transformation of F. vesca were studied. The results showed that F. vesca see...In this paper, several factors that affect the efficiency of in vitro adventitious bud regeneration and Agrobacterium tumefaciens-mediated transformation of F. vesca were studied. The results showed that F. vesca seeds germination rate was the highest while seeds were cultured in water, and the germination rate was the lowest while seeds were cultured on MS medium supplemented with hormone; the germination rates that seeds cultured on two and three layers filter paper were higher than that seeds cultured on four and five layers filter paper. In vitro adventitious regeneration efficiency was affected by different explants types. The significant difference was existed between petioles and leaves. When using the same type explants, in vitro adventitious buds regeneration rate and the average number of buds per explant between Ruegen （RE） and Yellow Wonder （YW） had no significant difference. RE to Agrobacterium tumefaciens was more sensitive than YW. Using seedling leaves of RE and YW as materials, an efficient Agrobacterium-mediated transformation system was developed. In this system, the concentration of bacteria was OD600=0.5, the explants were immersed in bacteria broth for 9 min, the co-cultured time was 2 days, and had no pre-cultured time. The percentage of explants with resistant buds of RE and YW was compared. The putative transformed plants were confirmed by PCR.展开更多
文摘The development of transgenic cotton varieties resistant to bollworms has been a major success of applying plant genetic engineering technology to agriculture,evidenced by phenomenal increase in
文摘A novel rice gene OsAPT2,which encodes a putative adenine phosphoribosyl transferase(APRT),was cloned.Its full-length cDNA is 1125bp,composing an ORF encoding 212 amino acid residues and a stop cordon,a 5' UTR of 123 bp and a 3' UTR of 363 bp.The sequence data have been submitted to the DDBJ/EMBL/GenBank databases(accession number:AY238894).The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously reported APRTs.The genomic OsAPT2 gene contains 7 exons and 6 introns.Its total length is 4758 bp.Then,an antisense expression vector of the full-length OsAPT2 cDNA was constructed and transformed into rice variety Taibei309 by Agrobacterium tumefaciens mediated transformation method.In total,650 T0 transgenic plants were obtained based on both antibiotic screening and specific PCR identification.One hundred individuals of them were selected and planted in Hainan Island.From those 11 male sterile lines with seed-setting rate lower than 3% in bagged spike were obtained.Results suggest that OsAPT2 is involved in male sterility.Nine of the 11 male sterile lines were constitutive sterile lines;two of the 11 male sterile lines were thermo-sensitive genic male sterile lines,which may be useful in hybride rice breeding.
基金financially supported by the National Nature Science Foundation of China (Project No.31170645)Key Innovation Team of Zhejiang Province (No.2011R50024)
文摘Long-term fertility cycle,low self-compatibility and short flowering period limited the tea breeding by conventional method.It is necessary to apply biotechnology,especially genetic transformation method,to obtain new mutants and to accelerate tea breeding.This paper reviews the status and progress in genetic transformation and related influencing factors.It has been noticed that tea genetic transformation has advantages in getting useful target mutants.The genetic transformation by Agrobacterium-mediated and gene gun methods are usually used.However,further research should be focused on improvement of plant regeneration.
基金Sponsored by the Nature Science Foundation of Jiangsu(BK2009410)
文摘An orthogonal wavelet transform fractionally spaced blind equalization algorithm based on the optimization of genetic algorithm(WTFSE-GA) is proposed in viewof the lowconvergence rate,large steady-state mean square error and local convergence of traditional constant modulus blind equalization algorithm(CMA).The proposed algorithm can reduce the signal autocorrelation through the orthogonal wavelet transform of input signal of fractionally spaced blind equalizer,and decrease the possibility of CMA local convergence by using the global random search characteristics of genetic algorithm to optimize the equalizer weight vector.The proposed algorithm has the faster convergence rate and smaller mean square error compared with FSE and WT-FSE.The efficiency of the proposed algorithm is proved by computer simulation of underwater acoustic channels.
基金Project(20040533035) supported by the National Research Foundation for the Doctoral Program of Higher Education of ChinaProject (60874070) supported by the National Natural Science Foundation of China
文摘To preserve the original signal as much as possible and filter random noises as many as possible in image processing,a threshold optimization-based adaptive template filtering algorithm was proposed.Unlike conventional filters whose template shapes and coefficients were fixed,multi-templates were defined and the right template for each pixel could be matched adaptively based on local image characteristics in the proposed method.The superiority of this method was verified by former results concerning the matching experiment of actual image with the comparison of conventional filtering methods.The adaptive search ability of immune genetic algorithm with the elitist selection and elitist crossover(IGAE) was used to optimize threshold t of the transformation function,and then combined with wavelet transformation to estimate noise variance.Multi-experiments were performed to test the validity of IGAE.The results show that the filtered result of t obtained by IGAE is superior to that of t obtained by other methods,IGAE has a faster convergence speed and a higher computational efficiency compared with the canonical genetic algorithm with the elitism and the immune algorithm with the information entropy and elitism by multi-experiments.
基金No any funds were received from any agency for this study.
文摘Since the advent of transgenic technology,the incorporation of gene(s)encoding traits of economic importance in cotton is being practiced worldwide.However,factors like recalcitrant nature of cotton cultivars,in vitro regeneration via tissue culture(especially via somatic embryogenesis),genotype dependency,long and toilsome protocols impede the pace of development of transgenic cotton.Besides that,types and age of explants,media composition,plant growth regulators and other environmental factors affect in vitro cotton regeneration significantly.The studies of genetic control of in vitro regeneration in plants have elucidated the role of certain transcription factor genes that are induced and expressed during somatic embryogenesis.Among these transcription factors,BABY BOOM(BBM)plays a very important role in signal transduction pathway,leading to cell differentiation and somatic embryos formation.The role of BBM has been established in plant cell proliferation,growth and development even without exogenous growth regulators.This review intends to provide an informative summary of regeneration and transformation problems in cotton and the latest developments in utilization of BBM transcription factors in cotton.We believe that the use of BBM will not only ease cotton genetic improvement but will also accelerate cotton breeding programmes.
基金Supported by the Scientific Research fund of the Guangxi Zhuang Autonomous Region(GXNYRKS201601)
文摘In this paper, several factors that affect the efficiency of in vitro adventitious bud regeneration and Agrobacterium tumefaciens-mediated transformation of F. vesca were studied. The results showed that F. vesca seeds germination rate was the highest while seeds were cultured in water, and the germination rate was the lowest while seeds were cultured on MS medium supplemented with hormone; the germination rates that seeds cultured on two and three layers filter paper were higher than that seeds cultured on four and five layers filter paper. In vitro adventitious regeneration efficiency was affected by different explants types. The significant difference was existed between petioles and leaves. When using the same type explants, in vitro adventitious buds regeneration rate and the average number of buds per explant between Ruegen （RE） and Yellow Wonder （YW） had no significant difference. RE to Agrobacterium tumefaciens was more sensitive than YW. Using seedling leaves of RE and YW as materials, an efficient Agrobacterium-mediated transformation system was developed. In this system, the concentration of bacteria was OD600=0.5, the explants were immersed in bacteria broth for 9 min, the co-cultured time was 2 days, and had no pre-cultured time. The percentage of explants with resistant buds of RE and YW was compared. The putative transformed plants were confirmed by PCR.
