摘要
A novel rice gene OsAPT2,which encodes a putative adenine phosphoribosyl transferase(APRT),was cloned.Its full-length cDNA is 1125bp,composing an ORF encoding 212 amino acid residues and a stop cordon,a 5' UTR of 123 bp and a 3' UTR of 363 bp.The sequence data have been submitted to the DDBJ/EMBL/GenBank databases(accession number:AY238894).The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously reported APRTs.The genomic OsAPT2 gene contains 7 exons and 6 introns.Its total length is 4758 bp.Then,an antisense expression vector of the full-length OsAPT2 cDNA was constructed and transformed into rice variety Taibei309 by Agrobacterium tumefaciens mediated transformation method.In total,650 T0 transgenic plants were obtained based on both antibiotic screening and specific PCR identification.One hundred individuals of them were selected and planted in Hainan Island.From those 11 male sterile lines with seed-setting rate lower than 3% in bagged spike were obtained.Results suggest that OsAPT2 is involved in male sterility.Nine of the 11 male sterile lines were constitutive sterile lines;two of the 11 male sterile lines were thermo-sensitive genic male sterile lines,which may be useful in hybride rice breeding.
A novel rice gene OsAPT2,which encodes a putative adenine phosphoribosyl transferase(APRT),was cloned.Its full-length cDNA is 1125bp,composing an ORF encoding 212 amino acid residues and a stop cordon,a 5 UTR of 123 bp and a 3 UTR of 363 bp.The sequence data have been submitted to the DDBJ/EMBL/GenBank databases(accession number:AY238894).The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously reported APRTs.The genomic OsAPT2 gene contains 7 exons and 6 introns.Its total len...
作者
WENG Man-li1,WANG Wang1,ZHOU Chun-jiang1,QIAO li-xian1,WANG Wei1,FENG Yan-bin1,DUAN De-lin2,DENG Qi-yun3,WANG Bin1(1.The State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,CAS,Beijing,100101,China
2.Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China
3.Hunan Hybrid Rice Research Center,Changsha 410125,China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第S1期205-,共1页
Journal of Hunan Agricultural University(Natural Sciences)
