The no-cloning theorem has sparked considerable interest in achieving high-fidelity approximate quantum cloning.Most of the previous studies mainly focused on the cloning of single particle states,and cloning schemes ...The no-cloning theorem has sparked considerable interest in achieving high-fidelity approximate quantum cloning.Most of the previous studies mainly focused on the cloning of single particle states,and cloning schemes used there are incapable of cloning quantum entangled states in multipartite systems.Few schemes were proposed for cloning multiparticle states,which consume more entanglement resources with loss of qubits,and the fidelity of the cloned state is relatively low.In this paper,cloning schemes for bipartite and tripartite entangled states based on photonic quantum walk and entanglement swapping are proposed.The results show that according to the proposed schemes,two high-fidelity(up to 0.75)cloned states can be obtained with less quantum resource consumption.Because of the simple cloning steps,few quantum resources and high fidelity,these schemes are both efficient and feasible.Moreover,this cloning machine eliminates the need for tracing out cloning machine,thereby minimizing resource waste.展开更多
At the early stage,the transcriptome sequencing technique was used to detect the differentially expressed gene CsFK111 between vine cucumber and dwarf cucumber D0462.The gene was cloned,and bioinformatics software too...At the early stage,the transcriptome sequencing technique was used to detect the differentially expressed gene CsFK111 between vine cucumber and dwarf cucumber D0462.The gene was cloned,and bioinformatics software tools were used to analyze and predict the gene family and this gene.There were 30 members of the cucumber F-box gene family.The coding region of the cucumber CsFK111 gene was full-length 1314 bp,which encoded 437 amino acids and was predicted to be located in the nucleus.The protein encoded by this gene was a non-transmembrane protein,and the prediction of the secondary structure showed thatβ-lamellar structure and irregular crimp were dominant.A comparison of the phylogenetic tree showed that it was closest to cantaloupe and belonged to the same branch.The results provided a basis for future study on the regulation mechanism of the CsFK111 gene on cucumber dwarfing and also laid a foundation for further study of FBK family proteins.展开更多
Fiber initiation and early elongation areimportant developmental stages at which thefinal fiber number per seed is determined and thefibers show dramatically changes in cell shapeand gene expression.In order to identi...Fiber initiation and early elongation areimportant developmental stages at which thefinal fiber number per seed is determined and thefibers show dramatically changes in cell shapeand gene expression.In order to identify genesfunction in fiber initiation and elongation,cDNA-AFLP technique was used to compare thegene expressions of the ovules of展开更多
According to the sequence of the bile salt hydrolase （BSH） gene of Bifidobacterium and the restriction enzyme cutting sites of expression vector pNZ8148, primers were designed and the bile salt hydrolase （BSH） gen...According to the sequence of the bile salt hydrolase （BSH） gene of Bifidobacterium and the restriction enzyme cutting sites of expression vector pNZ8148, primers were designed and the bile salt hydrolase （BSH） gene was gotten from Bacillus bifidus ATCC 29521 by PCR. BSH gene was inserted into lactic acid bacteria expression vector pNZ8148 to construct the recombinant pNZ8148-BSH. The recombinant pNZ8148-BSH was transferred into lactic acid bacteria NZ9000 with electrotransformation method. And the recombinant which could express BSH protein was obtained. It was identified by SDS-PAGE electrophoresis and activity verification. The result could provide a rationale reference for expressing BSH in lactic acid bacteria.展开更多
Chinese isolate of transmissible gastroenteritis virus(TGEV)was propagated and harvested in swine testicle(ST)cells.Two pairs of primers were designed according to the published sequence with Oligo 4.1 and DNasis soft...Chinese isolate of transmissible gastroenteritis virus(TGEV)was propagated and harvested in swine testicle(ST)cells.Two pairs of primers were designed according to the published sequence with Oligo 4.1 and DNasis softwares.The products of RT-PCR were named Sa and Sb,of 2.3kb and 2.1kb respectively.Sa was inserted in EcoR I and Kpn I sites after Sb was cloned in Kpn I and Pst I sites of the same pUC18 plasmid.The recombinant designated pUC-S was verified and analyzed by corresponding restriction endonuclease(RE)and nested PCR on the basis of genetic sites of S gene and physical map of pUC18 plasmid,which was identified as S gene from Chinese isolate of TGEV.展开更多
We cloned and expressed bile salt hydrolase gene ofLactobacillus plantarum M1-UVS29 in Lactococcus lactis NZ9000 successfully. Gene-specific primers for amplification of L. plantarum bsh were designed by using sequenc...We cloned and expressed bile salt hydrolase gene ofLactobacillus plantarum M1-UVS29 in Lactococcus lactis NZ9000 successfully. Gene-specific primers for amplification of L. plantarum bsh were designed by using sequence which availabled from GenBank. The production of PCR amplicon was confirmed by sequencing and cloned into pMD18-T vector, and then recombined into expression vector pNZ8148 and yielding vector pNZ8148-BSH, pNZ8148-BSH was transferred into Lactococcus lactis NZ9000. Sequencing indicated that the cloned bsh fragment contained 995 nucleotides, and shared 99.3% sequence homology with bsh gene from L. plantarum MBUL10. Cloned bsh fragment was successfully transduced into NICE expression system and confirmed by PCR and restriction digest. Recombinant BSH protein was analyzed by SDS-PAGE. The molecular weight of BSH protein was approximately 37 ku. Activity of the expressed protein was 0.77 μmol· min^-1. The successfully expressed proteins by genetic engineering technology made the function of lactic acid bacteria be abundant and laid the foundation for further researches into cholesterol-lowering lactic acid bacterium food and probiotics.展开更多
The purpose of this study is to find a new gene resource for the researches of molecular breeding of Rosaceae plants disease-resistance. Pyrus ussuriensis Maxim is used as a starting material to clone the full-length ...The purpose of this study is to find a new gene resource for the researches of molecular breeding of Rosaceae plants disease-resistance. Pyrus ussuriensis Maxim is used as a starting material to clone the full-length cDNA of NPR1（nonexpressor of pathogenesis- related genes 1） which is a key regulator in SA （salicylic acid）-mediated systemic acquired resistance （SAR） by homologous cloning and RACE techniques. The length of the cDNA sequence was 1 767 bp, the ORF was 1 761 bp, it coded 586 amino acids, pi=5.58, the relative molecular weight was 65.009 ku, contained 19 kinds of amino acids, and had full BTB/POZ and ANK domains. Compared the homology of NPR1 gene in GenBank database, the homology with Pyrus pyrifolia, Arabidopsis thaliana, Nicotiana tabacum, Lycopersicon esculentum, Oryza sativa, Helianthus annuus were 98%, 62%, 68%, 65%, 57%, 63%. The homology offunctional area were 99%, 78%, 82%, 79%, 74%, 77%. This NPR1 gene was considered as homologic gene of Pyrus ussuriensis Maxim and named PumNPR1.展开更多
Our current project is to isolate,identify andcharacterize cotton fiber-specific genes in orderto pick up candidates for fiber qualityimprovement.Firstly,10DPA(day postanthesis)cotton fiber cDNA library wasconstructed...Our current project is to isolate,identify andcharacterize cotton fiber-specific genes in orderto pick up candidates for fiber qualityimprovement.Firstly,10DPA(day postanthesis)cotton fiber cDNA library wasconstructed with 5X106 primary titer and 1.展开更多
Trehalose synthase is an important functional enzyme in the synthesis of trehalose in organisms and also participates in plant stress-resistant physiological processes.The transcriptomic study showed that a trehalose-...Trehalose synthase is an important functional enzyme in the synthesis of trehalose in organisms and also participates in plant stress-resistant physiological processes.The transcriptomic study showed that a trehalose-6-phosphate synthase gene was responsive to salt and alkaline stresses in Glycine soja.To dissect the molecular mechanisms of this enzyme in plant responses to stresses,the PCR technique was used to clone a trehalose-6-phosphate synthase gene from Glycine soja and it was designated as the GsTPS9.The full-length cDNA of this gene was 2583bp which encoded 861 amino acids.The sequence and structure analyses indicated that the GsTPS9 had high homology with Glycine max GmTPS9.The qRT-PCR analysis revealed that the GsTPS9 gene was expressed in Glycine soja roots,stems and leaves,and the highest expression level was in roots;the GsTPS9 gene had different responses under the stresses of NaCl,NaHCO_(3),PEG6000,ABA,MeJA and SA.This study laid the foundation for revealing the mechanism of the TPS in plant signal transduction pathways.展开更多
A predicted tau glutathione S-transferase(GST) subunit encoding gene,named GhGST,was isolated from Gossypium hirsutum with RACE method from SSH library based on Verticillium
Recent studies suggest that MHC class Ⅱ genetransfer could stimulate protective antitumor immunity.Whether xenogeneic MHC Ⅱ transfected tumor cells havethese effect is unknown. In this study, HLA-DR3 α and βchain ...Recent studies suggest that MHC class Ⅱ genetransfer could stimulate protective antitumor immunity.Whether xenogeneic MHC Ⅱ transfected tumor cells havethese effect is unknown. In this study, HLA-DR3 α and βchain cDNA were cloned by RT-PCR from human Blymphoma Raji cell line and confirmed by DNAsequencing. The recombinant expressing vectors wereconstructed by inserting the α and β chain cDNA intoPCEP4/pLXSN vectors respectively. After transfectingby lipofectamine and selecting with G418 and Hyg, theHLA-DR3 transfected mouse melanoma B16 recombinantclones were obtained. Approximately 59% of展开更多
LIM-domain proteins are implicated in multiplecellular and developmental processes ineukaryotes.Their essential roles have been wellcharacterized in animals.It was revealed thatLIM-domain protein plays an important ro...LIM-domain proteins are implicated in multiplecellular and developmental processes ineukaryotes.Their essential roles have been wellcharacterized in animals.It was revealed thatLIM-domain protein plays an important role invarious cellular processes,includingconstruction of cytoskeleton。展开更多
Verticillium wilt,caused by V.dahaliae,is aserious fungus disease of cotton in China.Nearly all cultivated upland cotton(Gossypiumhirsutum)varieties are sensitive to it.Somespecies of island cotton(G.barbadense),howev...Verticillium wilt,caused by V.dahaliae,is aserious fungus disease of cotton in China.Nearly all cultivated upland cotton(Gossypiumhirsutum)varieties are sensitive to it.Somespecies of island cotton(G.barbadense),however,have a natural resistance to thispathogen.To investigate the mechanism of展开更多
Brassinosteroids(BRs)are natural growth-promoting products found at low levels inpollen,seeds,and young vegatative tissuesthroughout the plant kingdom.Recently,thenotion that BRs are essential for plant growthand deve...Brassinosteroids(BRs)are natural growth-promoting products found at low levels inpollen,seeds,and young vegatative tissuesthroughout the plant kingdom.Recently,thenotion that BRs are essential for plant growthand development has been widely accepted bythe discovery of BR dwarf mutants展开更多
Specific primers for the MC1R gene of alpacas(GenBank EU1358800) were designed to amplify the cDNA sequence using RT-PCR to seek variation in the sequence and explore the relationship between the expression level of M...Specific primers for the MC1R gene of alpacas(GenBank EU1358800) were designed to amplify the cDNA sequence using RT-PCR to seek variation in the sequence and explore the relationship between the expression level of MC1R gene and alpaca coat color.The MC1R gene from white alpaca was cloned successfully and sequence analysis verified that the MC1R gene,encoding 317 amino acids,was 1081 bp in length.Compared with the existing sequence in GenBank,sequence identity was 99.9%and 7 mutations were found.Primers,designed from the sequence obtained,were used to assess the relative expression of MC1R in alpacas of different coat color using QRT-PCR and SPSS 13.0 software.Relative expression of MC1R in the skin of brown alpacas was 4.32 times higher than that in white alpacas after normalization with GAPDH(P【0.01),indicating that MC1R expression may be related to coat color of alpacas.展开更多
A novel rice gene OsAPT2,which encodes a putative adenine phosphoribosyl transferase(APRT),was cloned.Its full-length cDNA is 1125bp,composing an ORF encoding 212 amino acid residues and a stop cordon,a 5' UTR of ...A novel rice gene OsAPT2,which encodes a putative adenine phosphoribosyl transferase(APRT),was cloned.Its full-length cDNA is 1125bp,composing an ORF encoding 212 amino acid residues and a stop cordon,a 5' UTR of 123 bp and a 3' UTR of 363 bp.The sequence data have been submitted to the DDBJ/EMBL/GenBank databases(accession number:AY238894).The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously reported APRTs.The genomic OsAPT2 gene contains 7 exons and 6 introns.Its total length is 4758 bp.Then,an antisense expression vector of the full-length OsAPT2 cDNA was constructed and transformed into rice variety Taibei309 by Agrobacterium tumefaciens mediated transformation method.In total,650 T0 transgenic plants were obtained based on both antibiotic screening and specific PCR identification.One hundred individuals of them were selected and planted in Hainan Island.From those 11 male sterile lines with seed-setting rate lower than 3% in bagged spike were obtained.Results suggest that OsAPT2 is involved in male sterility.Nine of the 11 male sterile lines were constitutive sterile lines;two of the 11 male sterile lines were thermo-sensitive genic male sterile lines,which may be useful in hybride rice breeding.展开更多
Nine strains resistant to five fluoroquinolones (Ciprofloxacin, Ofloxacin, Enrofloxacin, Danofloxacin, Sarafloxacin) were isolated from clinical samples and extracted the chromosomal DNA of these strains. Designed pri...Nine strains resistant to five fluoroquinolones (Ciprofloxacin, Ofloxacin, Enrofloxacin, Danofloxacin, Sarafloxacin) were isolated from clinical samples and extracted the chromosomal DNA of these strains. Designed primers to amplify the Quinolone-resistance-determining region(QRDR) of gyrB gene, then the PCR products were cloned and the sequence was analyzed. In comparison with the standarded strain NCTC5776, no mutation was found in the QRDR of gyrB gene of all resistant strains. The result indicated that the QRDR of gyrB has little relationship with fluoroquinolone resistance to salmonella.展开更多
Chitin is the most widespread amino polysaccharide in nature. Chitin synthase （CHS） plays an important role in chitin formation in the cuticle and the peritrophic membrane （PM） lining the midgut. Total RNA was iso...Chitin is the most widespread amino polysaccharide in nature. Chitin synthase （CHS） plays an important role in chitin formation in the cuticle and the peritrophic membrane （PM） lining the midgut. Total RNA was isolated from the cuticle of Mamestra brassicae （L.） fourth instar larva, cDNA sequence was cloned by RT-PCR and Rapid Amplification of cDNA Ends （RACE）. cDNA 5 220 bp in length, contained an open reading frame of 4 704 bp coding for a polypeptide of 1 567 amino acid residues with a predicted molecular weight of 178.3 ku and its pI was 6.42. The deduced amino acid sequence from Mi brassicae （L.） shared the high level of identity with chitin synthase sequences from other insects, especially lepidopteran insects, cDNA sequence has been deposited with GenBank under accession No. GQ281761展开更多
Plants have developed a complicated defense mechanism during evolution to resist the harmful pathogens they encountered.The mechanism involves the interaction of the plant resistance(R)
文摘The no-cloning theorem has sparked considerable interest in achieving high-fidelity approximate quantum cloning.Most of the previous studies mainly focused on the cloning of single particle states,and cloning schemes used there are incapable of cloning quantum entangled states in multipartite systems.Few schemes were proposed for cloning multiparticle states,which consume more entanglement resources with loss of qubits,and the fidelity of the cloned state is relatively low.In this paper,cloning schemes for bipartite and tripartite entangled states based on photonic quantum walk and entanglement swapping are proposed.The results show that according to the proposed schemes,two high-fidelity(up to 0.75)cloned states can be obtained with less quantum resource consumption.Because of the simple cloning steps,few quantum resources and high fidelity,these schemes are both efficient and feasible.Moreover,this cloning machine eliminates the need for tracing out cloning machine,thereby minimizing resource waste.
基金Supported by the National Natural Science Foundation of China(32272724)the National Science Foundation of Heilongjiang Province,China(LH2019C033)。
文摘At the early stage,the transcriptome sequencing technique was used to detect the differentially expressed gene CsFK111 between vine cucumber and dwarf cucumber D0462.The gene was cloned,and bioinformatics software tools were used to analyze and predict the gene family and this gene.There were 30 members of the cucumber F-box gene family.The coding region of the cucumber CsFK111 gene was full-length 1314 bp,which encoded 437 amino acids and was predicted to be located in the nucleus.The protein encoded by this gene was a non-transmembrane protein,and the prediction of the secondary structure showed thatβ-lamellar structure and irregular crimp were dominant.A comparison of the phylogenetic tree showed that it was closest to cantaloupe and belonged to the same branch.The results provided a basis for future study on the regulation mechanism of the CsFK111 gene on cucumber dwarfing and also laid a foundation for further study of FBK family proteins.
文摘Fiber initiation and early elongation areimportant developmental stages at which thefinal fiber number per seed is determined and thefibers show dramatically changes in cell shapeand gene expression.In order to identify genesfunction in fiber initiation and elongation,cDNA-AFLP technique was used to compare thegene expressions of the ovules of
基金Supported by 863 Projects (2008AA10Z311)National Science and Technology Support Projects (2009BADB9B06)+1 种基金Started Post-doctoral Research Grant of Heilongjiang Province (LBH-Q07023)Harbin Technological Innovation of Special Funds (2007RFQXN020)
文摘According to the sequence of the bile salt hydrolase （BSH） gene of Bifidobacterium and the restriction enzyme cutting sites of expression vector pNZ8148, primers were designed and the bile salt hydrolase （BSH） gene was gotten from Bacillus bifidus ATCC 29521 by PCR. BSH gene was inserted into lactic acid bacteria expression vector pNZ8148 to construct the recombinant pNZ8148-BSH. The recombinant pNZ8148-BSH was transferred into lactic acid bacteria NZ9000 with electrotransformation method. And the recombinant which could express BSH protein was obtained. It was identified by SDS-PAGE electrophoresis and activity verification. The result could provide a rationale reference for expressing BSH in lactic acid bacteria.
文摘Chinese isolate of transmissible gastroenteritis virus(TGEV)was propagated and harvested in swine testicle(ST)cells.Two pairs of primers were designed according to the published sequence with Oligo 4.1 and DNasis softwares.The products of RT-PCR were named Sa and Sb,of 2.3kb and 2.1kb respectively.Sa was inserted in EcoR I and Kpn I sites after Sb was cloned in Kpn I and Pst I sites of the same pUC18 plasmid.The recombinant designated pUC-S was verified and analyzed by corresponding restriction endonuclease(RE)and nested PCR on the basis of genetic sites of S gene and physical map of pUC18 plasmid,which was identified as S gene from Chinese isolate of TGEV.
基金Supported by the National Natural Science Fund Project(31171657)Heilongjiang Province Natural Fund Project(ZD201207)Heilongjiang Province Postdoctoral Special Funds(LBH-Q13133)
文摘We cloned and expressed bile salt hydrolase gene ofLactobacillus plantarum M1-UVS29 in Lactococcus lactis NZ9000 successfully. Gene-specific primers for amplification of L. plantarum bsh were designed by using sequence which availabled from GenBank. The production of PCR amplicon was confirmed by sequencing and cloned into pMD18-T vector, and then recombined into expression vector pNZ8148 and yielding vector pNZ8148-BSH, pNZ8148-BSH was transferred into Lactococcus lactis NZ9000. Sequencing indicated that the cloned bsh fragment contained 995 nucleotides, and shared 99.3% sequence homology with bsh gene from L. plantarum MBUL10. Cloned bsh fragment was successfully transduced into NICE expression system and confirmed by PCR and restriction digest. Recombinant BSH protein was analyzed by SDS-PAGE. The molecular weight of BSH protein was approximately 37 ku. Activity of the expressed protein was 0.77 μmol· min^-1. The successfully expressed proteins by genetic engineering technology made the function of lactic acid bacteria be abundant and laid the foundation for further researches into cholesterol-lowering lactic acid bacterium food and probiotics.
文摘The purpose of this study is to find a new gene resource for the researches of molecular breeding of Rosaceae plants disease-resistance. Pyrus ussuriensis Maxim is used as a starting material to clone the full-length cDNA of NPR1（nonexpressor of pathogenesis- related genes 1） which is a key regulator in SA （salicylic acid）-mediated systemic acquired resistance （SAR） by homologous cloning and RACE techniques. The length of the cDNA sequence was 1 767 bp, the ORF was 1 761 bp, it coded 586 amino acids, pi=5.58, the relative molecular weight was 65.009 ku, contained 19 kinds of amino acids, and had full BTB/POZ and ANK domains. Compared the homology of NPR1 gene in GenBank database, the homology with Pyrus pyrifolia, Arabidopsis thaliana, Nicotiana tabacum, Lycopersicon esculentum, Oryza sativa, Helianthus annuus were 98%, 62%, 68%, 65%, 57%, 63%. The homology offunctional area were 99%, 78%, 82%, 79%, 74%, 77%. This NPR1 gene was considered as homologic gene of Pyrus ussuriensis Maxim and named PumNPR1.
文摘Our current project is to isolate,identify andcharacterize cotton fiber-specific genes in orderto pick up candidates for fiber qualityimprovement.Firstly,10DPA(day postanthesis)cotton fiber cDNA library wasconstructed with 5X106 primary titer and 1.
基金Supported by the National Natural Science Foundation of China(31670272)Heilongjiang Provincial Natural Science Foundation(C2017014)。
文摘Trehalose synthase is an important functional enzyme in the synthesis of trehalose in organisms and also participates in plant stress-resistant physiological processes.The transcriptomic study showed that a trehalose-6-phosphate synthase gene was responsive to salt and alkaline stresses in Glycine soja.To dissect the molecular mechanisms of this enzyme in plant responses to stresses,the PCR technique was used to clone a trehalose-6-phosphate synthase gene from Glycine soja and it was designated as the GsTPS9.The full-length cDNA of this gene was 2583bp which encoded 861 amino acids.The sequence and structure analyses indicated that the GsTPS9 had high homology with Glycine max GmTPS9.The qRT-PCR analysis revealed that the GsTPS9 gene was expressed in Glycine soja roots,stems and leaves,and the highest expression level was in roots;the GsTPS9 gene had different responses under the stresses of NaCl,NaHCO_(3),PEG6000,ABA,MeJA and SA.This study laid the foundation for revealing the mechanism of the TPS in plant signal transduction pathways.
文摘A predicted tau glutathione S-transferase(GST) subunit encoding gene,named GhGST,was isolated from Gossypium hirsutum with RACE method from SSH library based on Verticillium
文摘Recent studies suggest that MHC class Ⅱ genetransfer could stimulate protective antitumor immunity.Whether xenogeneic MHC Ⅱ transfected tumor cells havethese effect is unknown. In this study, HLA-DR3 α and βchain cDNA were cloned by RT-PCR from human Blymphoma Raji cell line and confirmed by DNAsequencing. The recombinant expressing vectors wereconstructed by inserting the α and β chain cDNA intoPCEP4/pLXSN vectors respectively. After transfectingby lipofectamine and selecting with G418 and Hyg, theHLA-DR3 transfected mouse melanoma B16 recombinantclones were obtained. Approximately 59% of
文摘LIM-domain proteins are implicated in multiplecellular and developmental processes ineukaryotes.Their essential roles have been wellcharacterized in animals.It was revealed thatLIM-domain protein plays an important role invarious cellular processes,includingconstruction of cytoskeleton。
文摘Verticillium wilt,caused by V.dahaliae,is aserious fungus disease of cotton in China.Nearly all cultivated upland cotton(Gossypiumhirsutum)varieties are sensitive to it.Somespecies of island cotton(G.barbadense),however,have a natural resistance to thispathogen.To investigate the mechanism of
文摘Brassinosteroids(BRs)are natural growth-promoting products found at low levels inpollen,seeds,and young vegatative tissuesthroughout the plant kingdom.Recently,thenotion that BRs are essential for plant growthand development has been widely accepted bythe discovery of BR dwarf mutants
基金supported by the National Natural Science Foundation of China(No.30501070)Shanxi Natural Science Foundation(No.20041099)President Foundation of Agricultural University of Hebei (BS2007023)
文摘Specific primers for the MC1R gene of alpacas(GenBank EU1358800) were designed to amplify the cDNA sequence using RT-PCR to seek variation in the sequence and explore the relationship between the expression level of MC1R gene and alpaca coat color.The MC1R gene from white alpaca was cloned successfully and sequence analysis verified that the MC1R gene,encoding 317 amino acids,was 1081 bp in length.Compared with the existing sequence in GenBank,sequence identity was 99.9%and 7 mutations were found.Primers,designed from the sequence obtained,were used to assess the relative expression of MC1R in alpacas of different coat color using QRT-PCR and SPSS 13.0 software.Relative expression of MC1R in the skin of brown alpacas was 4.32 times higher than that in white alpacas after normalization with GAPDH(P【0.01),indicating that MC1R expression may be related to coat color of alpacas.
文摘A novel rice gene OsAPT2,which encodes a putative adenine phosphoribosyl transferase(APRT),was cloned.Its full-length cDNA is 1125bp,composing an ORF encoding 212 amino acid residues and a stop cordon,a 5' UTR of 123 bp and a 3' UTR of 363 bp.The sequence data have been submitted to the DDBJ/EMBL/GenBank databases(accession number:AY238894).The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously reported APRTs.The genomic OsAPT2 gene contains 7 exons and 6 introns.Its total length is 4758 bp.Then,an antisense expression vector of the full-length OsAPT2 cDNA was constructed and transformed into rice variety Taibei309 by Agrobacterium tumefaciens mediated transformation method.In total,650 T0 transgenic plants were obtained based on both antibiotic screening and specific PCR identification.One hundred individuals of them were selected and planted in Hainan Island.From those 11 male sterile lines with seed-setting rate lower than 3% in bagged spike were obtained.Results suggest that OsAPT2 is involved in male sterility.Nine of the 11 male sterile lines were constitutive sterile lines;two of the 11 male sterile lines were thermo-sensitive genic male sterile lines,which may be useful in hybride rice breeding.
文摘Nine strains resistant to five fluoroquinolones (Ciprofloxacin, Ofloxacin, Enrofloxacin, Danofloxacin, Sarafloxacin) were isolated from clinical samples and extracted the chromosomal DNA of these strains. Designed primers to amplify the Quinolone-resistance-determining region(QRDR) of gyrB gene, then the PCR products were cloned and the sequence was analyzed. In comparison with the standarded strain NCTC5776, no mutation was found in the QRDR of gyrB gene of all resistant strains. The result indicated that the QRDR of gyrB has little relationship with fluoroquinolone resistance to salmonella.
基金Supported by Natural Science Foundation of Heilongjiang Province (C2007-7)Scientific and Technical Innovation Fund of Harbin (RC2006QN002027)Northeast Agricultural University Research Fund (2005)
文摘Chitin is the most widespread amino polysaccharide in nature. Chitin synthase （CHS） plays an important role in chitin formation in the cuticle and the peritrophic membrane （PM） lining the midgut. Total RNA was isolated from the cuticle of Mamestra brassicae （L.） fourth instar larva, cDNA sequence was cloned by RT-PCR and Rapid Amplification of cDNA Ends （RACE）. cDNA 5 220 bp in length, contained an open reading frame of 4 704 bp coding for a polypeptide of 1 567 amino acid residues with a predicted molecular weight of 178.3 ku and its pI was 6.42. The deduced amino acid sequence from Mi brassicae （L.） shared the high level of identity with chitin synthase sequences from other insects, especially lepidopteran insects, cDNA sequence has been deposited with GenBank under accession No. GQ281761
文摘Plants have developed a complicated defense mechanism during evolution to resist the harmful pathogens they encountered.The mechanism involves the interaction of the plant resistance(R)
