Catalyzed Resolutionb racemic 2-methyl-1-butanol was achieved,with max.95%optical purity of chiral alcohol,by candida cycindrace lipase in non-aqueous organic media.Effect of some factors on the resolution was investg...Catalyzed Resolutionb racemic 2-methyl-1-butanol was achieved,with max.95%optical purity of chiral alcohol,by candida cycindrace lipase in non-aqueous organic media.Effect of some factors on the resolution was investgated.The factors included temperature,pH,organic solvents,the amount of added water and the reaction time.展开更多
目的观察甲基化抑制剂5-氮杂-2′-脱氧胞苷(5-aza-2′-deoxycytidine,5-Aza-CdR)对肺癌SPC-A1细胞增殖、细胞划痕和凋亡的影响,探讨抑癌基因分泌型卷曲相关蛋白1(secreted frizzled related protein 1,SFRP1)和O6-甲基鸟嘌呤-DNA甲基转...目的观察甲基化抑制剂5-氮杂-2′-脱氧胞苷(5-aza-2′-deoxycytidine,5-Aza-CdR)对肺癌SPC-A1细胞增殖、细胞划痕和凋亡的影响,探讨抑癌基因分泌型卷曲相关蛋白1(secreted frizzled related protein 1,SFRP1)和O6-甲基鸟嘌呤-DNA甲基转移酶(O6-methylguanine-DNA-methyltransferase,MGMT)基因启动子区DNA甲基化mRNA和蛋白在其中的表达和意义。方法CCK-8法检测不同浓度的5-Aza-CdR对人肺癌SPC-A1细胞增殖影响,划痕实验测定5-Aza-CdR对SPC-A1细胞迁移能力的影响,Hoechst 33258染色检测(0、3、10、30μmol·L^(-1))5-Aza-CdR处理肺癌SPC-A1细胞24h后细胞凋亡情况,RT-PCR、Western blot法检测SPC-A1细胞中SFRP1和MGMT的mRNA、蛋白表达。结果5-Aza-CdR可以浓度梯度的抑制肺癌SPC-A1细胞增殖,IC 50为21.2μmol·L^(-1);5-Aza-CdR(3、10、30μmol·L^(-1))作用48 h后,肺癌SPC-A1细胞划痕愈合分别为对照组的(92.4±2.6)%、(83.6±4.2)%、(76.7±4.5)%;5-Aza-CdR处理肺癌SPC-A1细胞后,出现典型的细胞凋亡形态学改变;不同浓度5-Aza-CdR(3、10、30μmol·L^(-1))处理SPC-A1细胞24 h后,SFRP1、MGMT mRNA和蛋白表达增加(P<0.05)。结论5-Aza-CdR可抑制肺癌SPC-A1细胞增殖,抑制肺癌细胞划痕修复和促进凋亡,可能与升高MGMT和SFRPl的甲基化表达有关。展开更多
文摘Catalyzed Resolutionb racemic 2-methyl-1-butanol was achieved,with max.95%optical purity of chiral alcohol,by candida cycindrace lipase in non-aqueous organic media.Effect of some factors on the resolution was investgated.The factors included temperature,pH,organic solvents,the amount of added water and the reaction time.
