Objective To investigate whether 2,3,5,4'-tetrahydroxystilbene-2-O-β-glucoside(TSG)ameliorated polycystic ovary syndrome(PCOS)-like characteristics by inhibiting inflammation.Methods PCOS models were established ...Objective To investigate whether 2,3,5,4'-tetrahydroxystilbene-2-O-β-glucoside(TSG)ameliorated polycystic ovary syndrome(PCOS)-like characteristics by inhibiting inflammation.Methods PCOS models were established by injecting subcutaneously with dehydroepiandrosterone into female Sprague-Dawley rats,followed by receiving intraperitoneal injection of TSG.The granular cells(GCs)KGN were transfected with small interfering RNAs(si-NC and si-CYP19A1).The cells were preincubated with lipopolysaccharide(LPS)and then treated with or without TSG.The estrous cycle was monitored using vaginal exfoliated cells.The morphology of ovarian follicles was analyzed by H&E staining.ELISA was used to analyze estradiol(E2),testosterone(T),follicle stimulating hormone(FSH),luteinizing hormone(LH),IL-6,TNF-α,AGEs,CRP and Omentin-1 levels in serum.Immunohistochemistry was performed to analyze PCNA and CYP19A1 expressions in the GCs of ovaries.Tunel staining was executed to detect the apoptosis of GCs.Quantitative polymerase chain reaction(qPCR)and Western blot were implemented to measure the expression of CYP19A1 in the ovaries and transfected cells.qPCR was used to analyze the expression of IL-6 and TNF-αin the transfected cells treated with LPS and TSG.Results The estrous cycles were restored in TSG group.Compared with model group,the sinus follicles were reduced and corpus luteums were increased in TSG group.TSG group showed increased E2,and decreased T and LH,compared with model group.Pro-inflammatory factors(IL-6,TNF-α,CRP and AGEs)were decreased,and anti-inflammatory factor(Omentin-1)was increased in TSG group compared with those in model group.TSG could partially inhibit decrease of PNCA-positive GCs and increase of Tunel-positive GCs caused by PCOS.The CYP19A1 expression of GCs in TSG group was upregulated compared with model group.The expressions of IL-6 and TNFαin si-CYP19A1 cells were increased compared with si-NC cells.Compared with cells(si-NC and si-CYP19A1)treated without LPS,the expressions of IL-6 and TNF-αcells were increased,and the expression of CYP19A1 was downregulated in LPS-preincubated cells.Compared with cells treated with LPS,the expression of IL-6 and TNF-αwere decreased,and the expression of CYP19A1 was increased in cells treated with LPS and TSG.Compared with si-NC cells treated with LPS and TSG,the expressions of IL-6 and TNF-αcells were increased in the si-CYP19A1 cells treated with LPS and TSG.Conclusion TSG could alleviate PCOS-like characteristics by increasing the expression of CYP19A1 in GCs to inhibit inflammatory response.展开更多
目的:对比2017年美国放射学会甲状腺影像报告和数据系统(Thyroid imaging reporting and data system of ACR,ACR TI-RADS)与2011版Kwak甲状腺影像报告和数据系统(Thyroid imaging reporting and data system of Kwak,Kwak TI-RADS)在...目的:对比2017年美国放射学会甲状腺影像报告和数据系统(Thyroid imaging reporting and data system of ACR,ACR TI-RADS)与2011版Kwak甲状腺影像报告和数据系统(Thyroid imaging reporting and data system of Kwak,Kwak TI-RADS)在诊断甲状腺结节良恶性中的效能。方法:回顾分析305例患者319个甲状腺结节的常规超声图像结果,同时运用ACR TI-RADS和Kwak TI-RADS方法对上述结节进行分类,结果均以病理为参照标准进行统计学分析。结果:ACR TI-RADS诊断甲状腺结节良恶性的灵敏性为77.6%、特异性为83.0%、准确性为82.1%、阳性预测值为45.0%、阴性预测值为95.3%;Kwak TI-RADS诊断甲状腺结节良恶性的灵敏性为89.8%、特异性为81.1%、准确性为82.4%、阳性预测值为46.3%、阴性预测值为97.8%。对比显示两种方法无明显统计学差异。结论:①ACR TI-RADS和Kwak TI-RADS在甲状腺结节良恶性诊断中无明显差异,均具有良好的价值;②ACR TI-RADS进一步规范了甲状腺结节描述术语,提高了诊断一致性,并给予了甲状腺结节管理的建议。展开更多
文摘Objective To investigate whether 2,3,5,4'-tetrahydroxystilbene-2-O-β-glucoside(TSG)ameliorated polycystic ovary syndrome(PCOS)-like characteristics by inhibiting inflammation.Methods PCOS models were established by injecting subcutaneously with dehydroepiandrosterone into female Sprague-Dawley rats,followed by receiving intraperitoneal injection of TSG.The granular cells(GCs)KGN were transfected with small interfering RNAs(si-NC and si-CYP19A1).The cells were preincubated with lipopolysaccharide(LPS)and then treated with or without TSG.The estrous cycle was monitored using vaginal exfoliated cells.The morphology of ovarian follicles was analyzed by H&E staining.ELISA was used to analyze estradiol(E2),testosterone(T),follicle stimulating hormone(FSH),luteinizing hormone(LH),IL-6,TNF-α,AGEs,CRP and Omentin-1 levels in serum.Immunohistochemistry was performed to analyze PCNA and CYP19A1 expressions in the GCs of ovaries.Tunel staining was executed to detect the apoptosis of GCs.Quantitative polymerase chain reaction(qPCR)and Western blot were implemented to measure the expression of CYP19A1 in the ovaries and transfected cells.qPCR was used to analyze the expression of IL-6 and TNF-αin the transfected cells treated with LPS and TSG.Results The estrous cycles were restored in TSG group.Compared with model group,the sinus follicles were reduced and corpus luteums were increased in TSG group.TSG group showed increased E2,and decreased T and LH,compared with model group.Pro-inflammatory factors(IL-6,TNF-α,CRP and AGEs)were decreased,and anti-inflammatory factor(Omentin-1)was increased in TSG group compared with those in model group.TSG could partially inhibit decrease of PNCA-positive GCs and increase of Tunel-positive GCs caused by PCOS.The CYP19A1 expression of GCs in TSG group was upregulated compared with model group.The expressions of IL-6 and TNFαin si-CYP19A1 cells were increased compared with si-NC cells.Compared with cells(si-NC and si-CYP19A1)treated without LPS,the expressions of IL-6 and TNF-αcells were increased,and the expression of CYP19A1 was downregulated in LPS-preincubated cells.Compared with cells treated with LPS,the expression of IL-6 and TNF-αwere decreased,and the expression of CYP19A1 was increased in cells treated with LPS and TSG.Compared with si-NC cells treated with LPS and TSG,the expressions of IL-6 and TNF-αcells were increased in the si-CYP19A1 cells treated with LPS and TSG.Conclusion TSG could alleviate PCOS-like characteristics by increasing the expression of CYP19A1 in GCs to inhibit inflammatory response.
文摘目的:对比2017年美国放射学会甲状腺影像报告和数据系统(Thyroid imaging reporting and data system of ACR,ACR TI-RADS)与2011版Kwak甲状腺影像报告和数据系统(Thyroid imaging reporting and data system of Kwak,Kwak TI-RADS)在诊断甲状腺结节良恶性中的效能。方法:回顾分析305例患者319个甲状腺结节的常规超声图像结果,同时运用ACR TI-RADS和Kwak TI-RADS方法对上述结节进行分类,结果均以病理为参照标准进行统计学分析。结果:ACR TI-RADS诊断甲状腺结节良恶性的灵敏性为77.6%、特异性为83.0%、准确性为82.1%、阳性预测值为45.0%、阴性预测值为95.3%;Kwak TI-RADS诊断甲状腺结节良恶性的灵敏性为89.8%、特异性为81.1%、准确性为82.4%、阳性预测值为46.3%、阴性预测值为97.8%。对比显示两种方法无明显统计学差异。结论:①ACR TI-RADS和Kwak TI-RADS在甲状腺结节良恶性诊断中无明显差异,均具有良好的价值;②ACR TI-RADS进一步规范了甲状腺结节描述术语,提高了诊断一致性,并给予了甲状腺结节管理的建议。
