Estimation of support pressure is extremely important to the support system design and the construction safety of tunnels.At present,there are many methods for the estimation of support pressure based on different roc...Estimation of support pressure is extremely important to the support system design and the construction safety of tunnels.At present,there are many methods for the estimation of support pressure based on different rock mass classification systems,such as Q system,GSI system and RMR system.However,various rock mass classification systems are based on different tunnel geologic conditions in various regions.Therefore,each rock mass classification system has a certain regionality.In China,the BQ-Inex(BQ system)has been widely used in the field of rock engineering ever since its development.Unfortunately,there is still no estimation method of support pressure with BQ-index as parameters.Based on the field test data from 54 tunnels in China,a new empirical method considering BQ-Inex,tunnel span and rock weight is proposed to estimate the support pressure using multiple nonlinear regression analysis methods.And then the significance and necessity of support pressure estimation method for the safety of tunnel construction in China is explained through the comparison and analysis with the existing internationally widely used support pressure estimation methods of RMR system,Q system and GSI system.Finally,the empirical method of estimating the support pressure based on BQ-index was applied to designing the support system in the China’s high-speed railway tunnel—Zhengwan high-speed railway and the rationality of this method has been verified through the data of field test.展开更多
DUF761家族是植物中一个未知功能结构域蛋白家族(DUF,domains of unknown function),其在水稻中的功能从未被探索。本研究通过生物信息学分析在水稻全基因组共鉴定到33个水稻DUF761基因家族成员。系统发育分析将这些成员分为3个亚家族...DUF761家族是植物中一个未知功能结构域蛋白家族(DUF,domains of unknown function),其在水稻中的功能从未被探索。本研究通过生物信息学分析在水稻全基因组共鉴定到33个水稻DUF761基因家族成员。系统发育分析将这些成员分为3个亚家族。保守基序Motif-1分布于所有家族成员。在OsDUF761基因启动子区域共发现15种与胁迫响应、激素响应和发育相关的顺式作用元件。有30个基因的启动子区域含有ABRE元件(脱落酸响应),其中OsDUF761-21的启动子含有16个ABRE元件。组织表达谱检测结果表明,不同的OsDUF761基因成员在水稻中具有相似或者迥异的组织表达谱。在ABA、JA、低温、干旱和稻瘟菌分别处理水稻后,水稻中分别有12、13、24、24和18个OsDUF761基因的转录表达水平发生显著变化;其中,有8个基因在ABA处理和干旱处理后表现出共同的显著上调或者下调响应趋势;有6个基因在JA处理和稻瘟菌处理后表现出共同的响应趋势。本研究对水稻DUF761基因家族进行了全面分析,有助于进一步明确DUF761家族成员的生物学功能。展开更多
基金Projects(51878567,51878568,51578458)supported by the National Natural Science Foundation of ChinaProjects(2017G007-F,2017G007-H)supported by China Railway Science and Technology Research and Development Plan。
文摘Estimation of support pressure is extremely important to the support system design and the construction safety of tunnels.At present,there are many methods for the estimation of support pressure based on different rock mass classification systems,such as Q system,GSI system and RMR system.However,various rock mass classification systems are based on different tunnel geologic conditions in various regions.Therefore,each rock mass classification system has a certain regionality.In China,the BQ-Inex(BQ system)has been widely used in the field of rock engineering ever since its development.Unfortunately,there is still no estimation method of support pressure with BQ-index as parameters.Based on the field test data from 54 tunnels in China,a new empirical method considering BQ-Inex,tunnel span and rock weight is proposed to estimate the support pressure using multiple nonlinear regression analysis methods.And then the significance and necessity of support pressure estimation method for the safety of tunnel construction in China is explained through the comparison and analysis with the existing internationally widely used support pressure estimation methods of RMR system,Q system and GSI system.Finally,the empirical method of estimating the support pressure based on BQ-index was applied to designing the support system in the China’s high-speed railway tunnel—Zhengwan high-speed railway and the rationality of this method has been verified through the data of field test.
文摘DUF761家族是植物中一个未知功能结构域蛋白家族(DUF,domains of unknown function),其在水稻中的功能从未被探索。本研究通过生物信息学分析在水稻全基因组共鉴定到33个水稻DUF761基因家族成员。系统发育分析将这些成员分为3个亚家族。保守基序Motif-1分布于所有家族成员。在OsDUF761基因启动子区域共发现15种与胁迫响应、激素响应和发育相关的顺式作用元件。有30个基因的启动子区域含有ABRE元件(脱落酸响应),其中OsDUF761-21的启动子含有16个ABRE元件。组织表达谱检测结果表明,不同的OsDUF761基因成员在水稻中具有相似或者迥异的组织表达谱。在ABA、JA、低温、干旱和稻瘟菌分别处理水稻后,水稻中分别有12、13、24、24和18个OsDUF761基因的转录表达水平发生显著变化;其中,有8个基因在ABA处理和干旱处理后表现出共同的显著上调或者下调响应趋势;有6个基因在JA处理和稻瘟菌处理后表现出共同的响应趋势。本研究对水稻DUF761基因家族进行了全面分析,有助于进一步明确DUF761家族成员的生物学功能。
