Dendritic cells （DCs） are bone marrow-derived professional antigen presenting cells （APCs）, they are crucial for initiation of both innate and adaptive immune responses. In this study, chicken bone marrow （chBM）...Dendritic cells （DCs） are bone marrow-derived professional antigen presenting cells （APCs）, they are crucial for initiation of both innate and adaptive immune responses. In this study, chicken bone marrow （chBM） cells were cultured in medium with recombinant chicken granulocyte-macrophage colony stimulating factor （rGM-CSF） and recombinant chicken interleukin-4 （rIL-4） for 7 days, displayed the typical morphology of DCs. These immature chicken bone marrow-derived DCs （chBM-DCs） showed signifcant up-regulation of the putative CD11c and of major histocompatibility complex class II （MHC II）, but CD40 and CD86 co-stimulatory molecules were almost no up-regulated. However, maturation with lipopolysaccharide （LPS）, surface expression of CD40, CD86 was greatly increased. The phagocytosis of chBM-DCs was assessed by neutral red, and the phagocytosis decreased after stimulation. In mixed lymphocyte responses （MLR）, stimulated chBM-DCs were more effective to T-cell stimulators than non-stimulated chBM-DCs. In addition, mRNA expression levels of IL-1β, IL-4, IL-6, IL-10, IL-12, IFN-γ, TNF-α, CXCLi1 and CXCLi2 were assessed by real-time qPCR （qRT-PCR）, and the results showed cultured chBM-DCs could be matured to a T helper cell type 1 （Th1）-promoting phenotype by LPS stimulation.展开更多
基金Supported by the National Technology and Research Project(2015BAD12B01-4)
文摘Dendritic cells （DCs） are bone marrow-derived professional antigen presenting cells （APCs）, they are crucial for initiation of both innate and adaptive immune responses. In this study, chicken bone marrow （chBM） cells were cultured in medium with recombinant chicken granulocyte-macrophage colony stimulating factor （rGM-CSF） and recombinant chicken interleukin-4 （rIL-4） for 7 days, displayed the typical morphology of DCs. These immature chicken bone marrow-derived DCs （chBM-DCs） showed signifcant up-regulation of the putative CD11c and of major histocompatibility complex class II （MHC II）, but CD40 and CD86 co-stimulatory molecules were almost no up-regulated. However, maturation with lipopolysaccharide （LPS）, surface expression of CD40, CD86 was greatly increased. The phagocytosis of chBM-DCs was assessed by neutral red, and the phagocytosis decreased after stimulation. In mixed lymphocyte responses （MLR）, stimulated chBM-DCs were more effective to T-cell stimulators than non-stimulated chBM-DCs. In addition, mRNA expression levels of IL-1β, IL-4, IL-6, IL-10, IL-12, IFN-γ, TNF-α, CXCLi1 and CXCLi2 were assessed by real-time qPCR （qRT-PCR）, and the results showed cultured chBM-DCs could be matured to a T helper cell type 1 （Th1）-promoting phenotype by LPS stimulation.
