从库特氏杆菌发酵液中分离出可降解β-类胡萝卜素的酶,经初步纯化,研究其生化性质,为后续研究纯酶性质和应用打基础。以β-胡萝卜素标准品为底物,通过检测其反应最适温度、耐热性、反应最适pH、耐酸性、活化能、米氏常数,最大反应速率...从库特氏杆菌发酵液中分离出可降解β-类胡萝卜素的酶,经初步纯化,研究其生化性质,为后续研究纯酶性质和应用打基础。以β-胡萝卜素标准品为底物,通过检测其反应最适温度、耐热性、反应最适pH、耐酸性、活化能、米氏常数,最大反应速率等研究该类胡萝卜素降解酶的生化性质。试验结果表明:80℃为最适反应温度,具有较稳定的耐热性。该酶的耐酸性较强,在pH=3.0时活性最高。Fe^(2+)、Mn^(2+),Zn^(2+)抑制酶的活性,Al^(3+)、Fe^(3+),Mg^(2+),Ca^(2+)促进酶活性。由阿伦尼乌斯方程得酶反应的活化能Ea为230.3KJ/mol。在70℃和p H 3.0的条件下,其反应动力学常数Km为4.13 mmol/L,最大反应速率Vmax为1.1842umol/(L·min)。展开更多
A mono-complex has been synthesized by the reaction of 2,6-dicarboxamido-(2-pyridyl)-pyridine(H2pdcap) and CuCl2.X-ray diffraction technique reveals that the central Cu(II) ion lies in a distorted trigonal bipyramid g...A mono-complex has been synthesized by the reaction of 2,6-dicarboxamido-(2-pyridyl)-pyridine(H2pdcap) and CuCl2.X-ray diffraction technique reveals that the central Cu(II) ion lies in a distorted trigonal bipyramid geometry.The crystal is assigned to triclinic with space group Pī.The cell parameters are:a=0.8614(17);b=1.0621(2);c=1.0647(2);α=76.65(3)°;β=82.46(3)°;γ=82.58(3)°.The powder SHG effect of the title complex is 1.3 times than that of carbamide.展开更多
文摘从库特氏杆菌发酵液中分离出可降解β-类胡萝卜素的酶,经初步纯化,研究其生化性质,为后续研究纯酶性质和应用打基础。以β-胡萝卜素标准品为底物,通过检测其反应最适温度、耐热性、反应最适pH、耐酸性、活化能、米氏常数,最大反应速率等研究该类胡萝卜素降解酶的生化性质。试验结果表明:80℃为最适反应温度,具有较稳定的耐热性。该酶的耐酸性较强,在pH=3.0时活性最高。Fe^(2+)、Mn^(2+),Zn^(2+)抑制酶的活性,Al^(3+)、Fe^(3+),Mg^(2+),Ca^(2+)促进酶活性。由阿伦尼乌斯方程得酶反应的活化能Ea为230.3KJ/mol。在70℃和p H 3.0的条件下,其反应动力学常数Km为4.13 mmol/L,最大反应速率Vmax为1.1842umol/(L·min)。
文摘A mono-complex has been synthesized by the reaction of 2,6-dicarboxamido-(2-pyridyl)-pyridine(H2pdcap) and CuCl2.X-ray diffraction technique reveals that the central Cu(II) ion lies in a distorted trigonal bipyramid geometry.The crystal is assigned to triclinic with space group Pī.The cell parameters are:a=0.8614(17);b=1.0621(2);c=1.0647(2);α=76.65(3)°;β=82.46(3)°;γ=82.58(3)°.The powder SHG effect of the title complex is 1.3 times than that of carbamide.
