摘要
通过乙醇浸渍法从马尾松针中提取黄酮类化合物,采用正交实验确定了乙醇浓度、料液比、提取时间、提取温度等最佳的提取技术条件。提取后,回收乙醇,用水分散提取物,用石油醚去除叶绿素,采用正丁醇萃取,减压回收正丁醇,得到总黄酮。采用平板菌落计数法测定总黄酮的抑菌活性。结果表明,最佳提取条件为:乙醇浓度75%,材料比1:30 g/mL,提取时间90 min,提取温度90℃;主要和次要因素的影响是:乙醇浓度 】萃取时间 = 物质/液体比 】萃取温度。马尾松松针黄酮类化合物对沙门氏菌、大肠杆菌和金黄色葡萄球菌有抑制作用,其中对沙门氏菌的抑制作用最强。
Flavonoids were extracted from Pinus massoniana needles by ethanol impregnation, and the opti-mal extraction technical conditions such as ethanol concentration, ratio of material to liquid, ex-traction time, and extraction temperature were determined by orthogonal experiments. After ex-traction, ethanol was recovered, the extract was dispersed with water, chlorophyll was removed with petroleum ether, n-butanol was extracted using n-butanol, and n-butanol was recovered under reduced pressure to obtain total flavonoids. The antibacterial activity of total flavonoids was deter-mined by plate colony counting. The results showed that the optimum extraction conditions were as follows: ethanol concentration 75%, material ratio 1:30 g/mL, extraction time 90 min, extraction temperature 90˚C;the effects of main and secondary factors were as follows: ethanol concentration >extraction time = substance/liquid ratio >extraction temperature. Pinus massoniana flavonoids in-hibited Salmonella, E. coli and S. aureus, with the strongest inhibition against Salmonella.
出处
《有机化学研究》
2021年第3期23-31,共9页
Journal of Organic Chemistry Research
