摘要
实验观察到锰 氧化抗坏血酸可以产生弱的化学发光 ,甲醛对这一化学发光反应有较强的增敏作用 ,据此建立了测定抗坏血酸的流动注射化学发光分析法。方法的检出限为 2× 1 0 -8mol/L抗坏血酸 ;线性响应范围为 6 .0× 1 0 -8～ 2 .0× 1 0 -5mol/L。对 1 .0× 1 0 -6mol/L的抗坏血酸进行 1 1次测定 ,相对标准偏差为 2 3%。该方法已成功应用于维生素C针剂和片剂中抗坏血酸含量的测定 。
A novel chemiluminescence method to determine ascorbic acid with flow injection technique, based on formaldehyde enhancing the chemiluminescence from the oxidation of ascorbic acid by soluble Mn , has been developed. Under optimum conditions, the linear dynamic range for the determination of ascorbic acid was from 6.0×10 -8 to 2.0×10 -5 mol/L, and the detection limit was 2×10 -8 mol/L. The relative standard deviation for the determination of 1.0×10 -6 mol/L ascorbic acid ( n =11) was 2.3%. The method has been applied to the determination of ascorbic acid in tablets and injections and the results were in good agreement with those obtained by pharmacopoeia method.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2004年第6期752-754,共3页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助项目 (No .2 0 2 75 0 2 3 )
