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海洋真菌LD8岩藻多糖酶的固态发酵条件研究 被引量：7

Solid state fermentation of fucoidanase of marine fungus LD8
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摘要 对海洋真菌LD8岩藻多糖酶的固态发酵条件进行了研究 ,主要内容包括氮源、诱导物、起始 pH、人工海水盐浓度及温度等。固态发酵最佳培养基组成 :麸皮7.5g,葡萄糖0.1g,海带粉0.5g,NaNO3 为0.04g;最佳培养条件为培养温度28℃ ,起始pH6.0。在28℃培养48h,酶活力可达13.6IU/g干培养基 ,比活力为0.93IU/mg蛋白质。另外对岩藻多糖酶酶学性质进行了研究。 Solid state fermentation conditions of LD8fucoidanase were studied.The optimal medium contained wheat bran7.5g,glucose0.1g,kelp0.5g and NaNO 3 0.04g.The optimal initial pH and temperature were6.0and28℃respectively.Under the above conditions,fucoidanase activity reached13.6IU/g dry medium after48h,and its specific activity was as high as0.93IU/mg.The fucoidanase properties of LD8were also investigated.
作者 吴茜茜 蔡敬民 吴克 秦松
机构地区 合肥学院 中国科学院海洋研究所
出处 《海洋科学》 CAS CSCD 北大核心 2004年第6期23-27,共5页 Marine Sciences
基金 教育部留学回国人员科研启动基金 安徽省优秀青年科技基金资助 (04043051) 安徽省自然科学基金项目(03043104) 中国科学院实验海洋生物学开放研究室研究报告第305号 合肥学院科学研究发展基金
关键词 岩藻多糖酶 固态发酵 酶学性质 海洋真菌 fucoidanase solid state fermentation properties marine fungi
分类号 Q55 [生物学—生物化学]
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参考文献15

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二级参考文献10

  • 1[1]Alain N, Frederic C, Catherine B V, 1991. Anticoagulant low molecular weight fucans produced by radical process and ion exchange chromatography of high molecular weight fucans extracted from the brown seaweed Ascophyllum modosum. Carbohydrate Research 289:201～208
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  • 10吴茜茜,吴克,蔡敬民,刘斌,严小军,秦松,潘仁瑞.海带岩藻多糖的分离与部分性质研究[J].食品与发酵工业,2001,27(10):39-42. 被引量：10

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二级引证文献27

海洋科学
2004年 第6期

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