摘要
目的 探讨高压氧 (HBO)在脑缺血再灌注损伤中对自由基的产生及血脑屏障 (BBB)通透性的影响。方法 昆明种小鼠 3 2 0只 ,随机分为假手术组、HBO组、脑缺血再灌注组和HBO +脑缺血再灌注组 ,每组各 80只。复制清醒脑缺血再灌注模型 ,并于术后经 0 .2 5MPaHBO治疗 5次 ,采用比色法检测各组小鼠海马组织SOD、CAT、GSH PX活性和MDA及EB的含量。取小鼠海马组织常规制片 ,透射电镜下观察并进行比较。结果 脑缺血再灌注组与假手术组比较 ,SOD、CAT及GSH PX活性明显下降 (P <0 .0 1) ,MDA含量明显升高 (P <0 .0 1) ,与脑部EB含量增高相一致。HBO +脑缺血再灌注组与脑缺血再灌注组相比 ,SOD、CAT、GSH PX活性明显升高 (P <0 .0 5 ) ,MDA含量明显下降 (P <0 .0 1) ,并与脑部EB含量减少相一致。HBO组与假手术组相比 ,GSH PX酶活性明显增高 (P <0 .0 5 )。电镜下示脑缺血再灌注组神经元坏死明显 ,星形胶质细胞胞体水肿 ,微管、微丝明显减少 ;HBO +脑缺血再灌注组神经元坏死不明显。结论 高压氧可增强脑缺血再灌小鼠脑组织抗氧化酶类活性 ,恢复星形胶质细胞功能 ,降低BBB通透性。
Objective To explore the effect of hyperbaric oxygenation (HBO) on generation of free radicals and the permeability of blood-brain barrier(BBB) during cerebral ischemia-reperfusion(CIR). Methods Three hundred and twenty Kunming mice were randomly divided into four groups: a sham operation group, a HBO group, a CIR group, and a HBO + CIR group, with 80 mice in each group. Conscious mice were used to establish the CIR model, and 0.25MPa (ATA) HBO were applied 5 times after operations. The activities of SOD, CAT, GSH-PX and the concentration of MDA, EB in the cerebral hippocampal tissues in each group were measured with colorimetry. The cerebral hippocampal tissues were harvested and processed, then observed and compared with transmission electron microscopic observation. Results Compared with the sham operation group, the activities of antioxidation enzymes (GSH-PX,SOD, CAT) in CIR group decreased significantly (P<0.01), while the concentration of MDA and EB increased significantly(P<0.01). Compared with the CIR group, the activities of antioxidation enzymes (GSH-PX,SOD, CAT)in the HBO + CIR group raised obviously, (P<0.01), while the concentration of MDA and EB lowered significantly(P<0.01). Compared with the sham operation group, the activity of GSH-PX in HBO group increased significantly (P<0.05). Meanwhile, in the CIR group, necrosis of neurons and edema of astrocyte were obvious, and the microtubule and microfilament of astrocyte decreased significantly. However, necrosis of neurons was not obvious in HBO + CIR group. Conclusion HBO could increase the activity of antioxidation enzymes, restore the function of astrocyte and decrease the permeability of BBB.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2004年第4期196-199,共4页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
高压氧
脑缺血
再灌注损伤
小鼠
抗氧化酶类
血脑屏障
星形胶质细胞
Antioxidation enzymes
Cerebral ischemia-reperfusion
Astrocyte
Hyperbaric oxygenation
Blood brain barrier
