摘要
本研究运用染色体显带技术对人肺腺癌细胞系PC84045进行了细胞遗传学研究。结果表明,该细胞系是一个亚三倍体,染色体众数为66,染色体数目和结构存在众多异常。多倍体细胞占19/144,C-后期核型占22/144。结构异常包括双着丝粒染色体、染色单体裂隙、染色体裂隙及染色体断片、三射体、粉碎化染色体等。还可见比染色体断片小得多的微小体。本文确定了该细胞系的7个标记染色体,涉及的主要断裂点包括3q11和3p21、1p11和1p13,12p13及7q32,并对这些断裂点在肿瘸发生中的意义进行了讨论。
By using chromosomal banding techniques, a cytogenetic analysis of the human lung adenocarcinoma cell line PC84045 was made. It was found that the chromosomal modal number of the cell line is 66, a hypotriploid. There are many chromosomal numerical and structural aberrations. Among 144 division cells, 19 are polyploids and 22 c-anaphase karyotypes. The types of structural aberrations are dicentric chromosomes, chromosome (chromatid) breaks (gaps), triradials, and chromosome pulverization. There are many microbodies much smaller than breakage chromosomes. We determine seven marker chromosomes in the cell line, and the main frequency breakpoints involved are 3qll and 3p21, 1ip11 and 1p13, 12P13 and 7q32 respectively. The role that the breakpoints play in carcinogenesis is discussed.
出处
《同济医科大学学报》
CAS
CSCD
北大核心
1992年第5期305-307,共3页
Acta Universitatis Medicinae Tongji
关键词
染色体
肺肿瘤
癌
lung cancer
chromosomal bancling
marker chromosome
