摘要
采用随机扩增多态性DNA(RAPD)技术 ,对豹猫 (Prionailurusbengalensis)的 6个群体进行了遗传多样性和系统发育分析。在 4 0个随机引物中筛选出 35个有效引物 ,对 6份群体样品扩增 ,共获得 2 88条DNA谱带 ,其中 2 31条具有多态性 ,占 80 .2 1%。单个引物获得的标记数在 2～ 16之间 ,平均每个引物获得 8.2 3条DNA谱带。基于遗传距离 ,用UPGMA法构建了系统聚类图。聚类分析显示 ,所研究豹猫的 6个群体可分 4支 ,黑龙江—山西群体为一支 ,对应于北方亚种 (P .b.euptilura) ;贵州—广西群体聚在一起为另一支 ,对应于指名亚种 (P .b .bengalensis) ;云南群体为第三支 ,对应于川西亚种 (P .b.scripta) ;福建群体为第四支 ,对应于华东亚种 (P .b .chinensis)。该结果支持目前基于形态和地理分布的亚种划分。
Genetic divergence and phylogenetic relationships among six populations of the leopard cat (Prionailurus bengalensis) in China were analyzed by Random Amplified Polymorphic DNAs (RAPD) methods. 35 effective primers were obtained from 40 random primers. 35 primers amplified 6 mixed samples representing 6 populations respectively and 288 DNA bands were obtained, of which 231 bands are polymorphic, accounting for 80.21%. The numbers of DNA bands produced by single primer range from 2 to 16. On average, each primer produces 8.23 DNA bands. Based on genetic distances, molecular phylogenetic trees of the leopard cat have been constructed by Unweighted Pair Group Method using Arithmetic Mean(UPGMA), 6 populations of the leopard cat can be divided into 4 clades: the Heilongjiang-Shanxi clade, the Guizhou-Guangxi clade, the Yunnan clade and the Fujian clade, which correspond to P. b. euptilura, P. b. bengalensis, P. b. scripta and P. b. chinensis, respectively. The results support the traditional taxonomy of leopard cat subspecies based on morphological characteristics and geographic distribution.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2004年第3期52-54,共3页
Journal of Northeast Forestry University
