摘要
使用合成的大环抗生素 去甲万古霉素键合手性固定相 (NVC CSP) ,在反相条件下直接拆分了 4种丹酰化氨基酸对映异构体 ,并系统考察了缓冲溶液 pH值、有机改性剂用量、温度以及流速对对映体拆分的影响。研究结果表明 ,在反相条件下 ,疏水作用和静电作用是影响溶质保留和手性选择性的重要因素 。
Using the newly synthesized norvancomycin chiral stationary phase (NVC-CSP), the enantiomers of dansyl-alpha-amino-n-butyric acid (Dns-But), dansyl-methionine (Dns-Met) dansyl-threonine (Dns-Thr) and dansyl-glutamic acid (Dns-Glu) were directly separated on reversed phase conditions. The influences of some chromatographic parameters, such as pH of buffer, the content of organic modifier, temperature and flow rate, were evaluated, and furthermore, the possible mechanism of chiral discrimination was discussed. It was found experimentally that hydrophobic, as well as ionic interactions were important for solute's retention and enantioselectivity in this chromatographic system, and the chiral recognition mechanism was similar to that of protein-based CSPs.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2004年第5期606-610,共5页
Chinese Journal of Analytical Chemistry
关键词
去甲万古霉素
手性固定耗
高效液相色谱
丹酰化氨基酸
生物大分子
药物
norwancomycin
chiral stationary phase
high performance liquid chromatography
chiral separation
dansyl-amino acids
