摘要
目的 了解昆明市轮状病毒腹泻的流行状况。方法 以昆明市儿童医院为哨点监测,监测对象为5岁以下腹泻住院患儿,收集患儿的临床资料和粪便标本进行轮状病毒的检测和分型。病毒检测用聚丙烯酰胺凝胶电泳(PAGE)和酶联免疫吸附试验(ELISA),毒株分型用ELISA和/或反转录-聚合酶链反应(RT-PCR)。结果 3年监测中共收集466份腹泻患儿的粪便标本,轮状病毒的检出率为52.8％(246/466)。轮状病毒感染97％发生于2岁以下儿童。感染有明显的季节性,10～12月份是流行季节。对204份轮状病毒阳性标本进行G分型,G1型为流行优势株,占47.5％,其次为G2型(17.6％)、G3型(15.7％)G9型(4.9％)和G4型(1.0％)。P基因型以P[4]、P[8]和P[6]型为常见。最常见的P-G组合型是P[4]G2,占34.1％(14/41),其次是P[8]G1和P[6]G9,分别占29.3％(12/41)和12.2％(5/41),还有其他7种不常见的P-G组合的毒株类型。结论 轮状病毒是昆明地区儿童腹泻住院的主要病原,毒株呈现型的多样性,应该开发和应用轮状病毒疫苗预防控制其流行。
Objective To study the epidemiological status on rotavirus diarrhea in Kunming to
improve the rotavirus vaccine immunization program. Methods A hospital-based sentinel surveillance
program for rotavirus was set up among children less than 5 years old with acute diarrhea in Kunming
Children's Hospital. Clinical information and fecal specimens were collected and rotavirus were detected by
polyacrylamide gel electrophoresis(PAGE) and/or enzyme linked immunosorbent assay (ELISA). Positive
specimens were further serotyped or genotyped by ELISA and/or RT-PCR. Results During the three years
of surveillance, 466 specimens were collected. Rotavirus were detected on 246 (52. 8%) specimens. 97%
of the rotavirus diarrhea cases occurred among children less than 2 years old. There was a peak of
admissions for rotavirus diarrhea cases between October and December which accounted for 48% of all the
rotavirus hospitalizations each year. Among 204 specimens with G serotyping, the predominant strain was
serotype G1(47.5%) followed by G2 (17. 6%), G3 (15.7%), G9 (4.9%) and G4(1.0%). Mixed
infection (2. 5%) were rare and 22 specimens (10.8%) remained non-typeable. P genotyping showed P
[4],P[8] and P[6] were the most common strains, accounting for 29. 3%, 27. 6% and 13.8%
respectively. P[4]G2 was the most common strain which accounted for 34. 1% (14/41) followed by P [8]
G1(29.3%) and P[6]G9 (12.2%). Another 7 uncommon P-G combinations were also identified.
Conclusion Rotavirus was the major cause of acute diarrhea in Kunming. An effective rotavirus vaccine for
prevention and control of rotavirus diarrhea should be developed.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2004年第5期396-399,共4页
Chinese Journal of Epidemiology
基金
世界卫生组织课题基金(271/18/123)
美国NIH课题基金(R03TW01192)
国家"863"计划基金项目(2001AA21217)
