摘要
萌发的水稻种子经42℃热激处理后其幼苗的耐冷性明显增强,膜伤害程度降低,脯氨酸含量增加,超氧物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性和抗氧化物质抗坏血酸含量增加,而膜脂过氧化的关键酶脂氧合酶(LOX)活性及其产物丙二醛(MDA)含量下降。并且热激诱导萌发的水稻胚合成78、70、64、60、46、38、24、17、16 kD的热激蛋白(HSP),其中属于HSP70的内质网结合蛋白(BiP)的合成与水稻幼苗耐寒性的提高有关。
Heat shock applied to germinated rice seeds increased the chilling tolerance of seedlings (Fig.1).Comparison with the control, brief heat shock applied before chilling at 4°C for 2 days and recovery at 25°C for another 2 days decreased the permeability of cellular membranes and increased the content of proline in rice seedlings(Fig.2A,B). Heat shock applied before chilling also increased the activities of superoxide dismutase, catalase, and peroxidase and the content of ascorbate in rice seedlings(Fig. 2C,Fig.3A,B,C). In contrast, the lipoxygenase activity and the malondialdehyde content in the heat-shocked rice seedlings were lower than those in the control(Fig.2D,Fig.3D). Germinated rice embryos synthesized heat shock proteins of Mr 78,70,64,46,38,24,17 and 16 kD during heat shock (Fig.5). The results of Western blot suggested that the binding protein (Bip) of HSP70 play an important role in protecting the rice seedling against chilling damage (Fig.6 and Fig.7).
出处
《植物生理与分子生物学学报》
CAS
CSCD
2004年第2期189-194,共6页
Journal Of Plant Physiology and Molecular Biology
基金
国家自然科学基金(No.30370912)资助~~
关键词
水稻
耐冷性
热激蛋白
抗氧化酶
rice
chilling tolerance
heat shock protein
antioxidase
