摘要
担子菌PM2在限氮液体培养下 ,分泌木质素过氧化物酶和锰过氧化物酶 ;藜芦醇、吐温 80的补充 ,提高了该菌锰过氧化物酶的产生 ,获得的最大锰过氧化物酶Mnp酶活为 2 54 2u L、190 2u L ,分别是对照的 3 4倍和 2 5倍。选择三种偶氮染料 ,在染料体系下 ,进一步分析藜芦醇、吐温 80对担子菌PM2产过氧化物酶及染料脱色的影响。结果表明 ,担子菌PM2分泌的锰过氧化物酶Mnp与染料脱色有关 ,脱色程度受其分子结构特征影响 ;吐温 80的补充 ,更有利于染料的脱色降解 ,48h后三种染料均可达到 80
Basidiomycete PM2, a lignin-degrading white rot fungus, produces lgnin peroxidase( Lip) and manganese peroxidase( Mnp) in nutrient nitrogen limited liq uid cultures. This fungus was selected for its ability to decolorize azo group o f dyes. In order to improve production of the peroxidases and rapid dye decolori zing activity by basidiomycete PM2, the addition of veratryl alcohol or Tween 80 to nutrient nitrogen limited liquid cultures were tested. It was found to have a large stimulatory effect on Mnp activities and decolorization rate of azo dyes . A maximum Mnp activities of 254 2u/L with veratryl alcohol and 192 2u/L with T ween 80 were achieved respectively. These values were about 3 4-fold and 2 5 -fol d higher than that obtained in the control cultures(without alcohol or Tween 80) , whereas the levels of Lip activity detected were very low(about 12u/L)in all t he cultures. In further experiments using three kinds of azo dyes of congo red, orange G and orange Ⅳ, enzyme activities and dye decolorization were investigat ed in the above-mentioned cultures. The results showed that Mnp activities and d ecolorization were notably higher than those obtained in the control cultures i n the presence of azo dyes. Cultures supplemented with Tween 80 were more adequa te for dye decolorization. The rates of the decolorization with Tween 80 of cong o red(95 4%) , orange G(98 5%) and orange Ⅳ(54 4%) after 24 hours of dye inc uba tion were higher than that supplemented with veratryl alcohol. According to the results, Mnp activities secreted by basidiomycete PM2 play an essential role in the process of dye decolorization. Tween 80 was the main factor affecting the de colorization. The analysis of structure of the three kinds of azo dyes indicats that the extent of decolorization is affected by the dye molecular structure. Th e types and quantity of the substituted groups on the aromatic ring of azo dyes have effect on the percentage of biological decolorization.
出处
《生物工程学报》
CAS
CSCD
北大核心
2004年第2期302-305,共4页
Chinese Journal of Biotechnology
基金
安徽省自然科学基金项目 (No 0 1 0 4 31 0 1 )
省教育厅科研项目 (No 2 0 0 1KJ00 9)~~
