摘要
大肠菌群广泛用作饮用水的细菌学检测指标。传统的检测方法有多管发酵法和滤膜法。这些方法存在一些缺点 ,如检测时间长、专一性差、干扰因素多。因此 ,迫切需要一些快速、灵敏的检测方法。FISH(fluorescentinsituhybridization)技术利用寡核苷酸探针检测特定细胞内的互补核苷酸序列。针对于Enterobacteriaceae的 1 6SrRNA分子区域专门设计的探针可以用于饮用水样品的微生物检测。FISH技术可以用于饮用水中大肠菌群的检测 ,并且在较短的时间内 ( 6～ 8h)给出定量分析结果 ,但该技术用于日常检测尚需更深入的研究。
The coliform group has been used extensively as an indicator of water quality.The traditional methods for coliform detection include the multiple-tube fermentation (MTF) technique and the membrane filter (MF) technique.These methods have several limitations,such as time-consuming,lack of specificity and various interferences.FISH technique involves the use of oligonucleotide probes to detect complementary sequences inside specific cells.Oligonucleotide probes designed specifically for regions of the 16S rRNA molecules of Enterobacteriaceae can be used for microbial detection of drinking water samples.FISH should be an interesting viable alternative to the conventional culture methods for the detection of coliforms in drinking water,as it provides quantitative data in a fairly short period of time (6～8h),but still requires further research works.;
出处
《中国生物工程杂志》
CAS
CSCD
2004年第2期70-73,共4页
China Biotechnology
基金
国家自然科学基金资助项目 ( 5 0 2 780 45 )
