摘要
目的 :观察蜂毒素微球 ( M- MS)经动脉介入对大鼠肝癌的治疗作用。方法 :采用改良的复乳 -液中干燥法制备蜂毒素 -聚乳酸 /羟乙酸微球 ,建立大鼠移植性肝癌模型并随机分为四组 ,分别经肝动脉灌注生理盐水 ( NS,1 .5 ml/kg)、蜂毒素 ( Melittin,0 .35 mg/kg)、空白微球 ( B- MS,1 0 mg/kg)和蜂毒素—聚乳酸 /羟乙酸微球 ( 1 0 mg/kg)。比较治疗后各组大鼠的肿瘤生长情况、肿瘤坏死程度和生存时间。结果 :与生理盐水组比较 ,Melittin组、B- MS组肿瘤生长受到明显抑制 ( P<0 .0 1 ) ,肿瘤坏死程度以轻中度为主 ,但两组动物生存时间均未能明显延长 ( P>0 .0 5 )。M- MS组与 NS组、Melittin组及 B- MS组相比 ,肿瘤生长抑制显著 ( P<0 .0 1 ) ,肿瘤坏死更广泛、更彻底 ,且经蜂毒素微球治疗的大鼠生存期显著延长 ( P<0 .0 1 )。结论 :蜂毒素以药物微球的剂型经肝动脉给药 ,抗肿瘤效果明显优于单纯的蜂毒素和空白微球。
Objective: To observe the curative effect of poly lactic-co-glycolic acid(PLGA) microspheres containing Melittin(M-MS) infused via artery against rats' hepatocarcinoma.Methods:M-MS were prepared with biodegradable PLGA by using improved mutiple emulsion in liquid evaporation process, then the models of rats bearing transplanted hepatocarcinoma were established. All rats were divided randomly into 4 groups, which were respectively injected via liver artery by NS(1.5,l/kg), Melittin(0.35mk/kg), B-MS(10mg/kg) and Melittin-PLGA(10mg/kg). To compare the rats' hepatocarcinoma growth, harm and living time.Results: Compared with NS group, The tumor growth was significantly suppressed in Melittin and B-MS group( P <0.01), and the living time in both later groups didn't prolonged significantly( P >0.05).Compared with NS group ,Melittin group and B-MS group, The tumor growth was suppressed significantly( P <0.01), its tissue was more completely hurt, and its living time got prolonged significantly( P <0.01). Conclusion:The curative effect of poly lactic-co-glycolic acid(PLGA) microspheres containing Melittin(M-MS) infused via liver artery against rats' hepatocarcinoma is obviously better than that of merely treated by Melittin or vacant microspheres.
出处
《浙江中西医结合杂志》
2004年第2期72-74,共3页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金
上海市"百人计划"资助课题
编号 :97BR0 44
军队九五重点课题
编号 :98JD-3 2 69
关键词
蜂毒素
微球
介入治疗
大鼠
肝癌
药理作用
rat hepatocarcinoma melittin microspheres poly lactic-co-glycolic acid infused through artery therapy
