摘要
目的:探讨微RNA(microRNA,miR)-1299在食管癌组织中的表达水平及其对食管癌TE1细胞迁移和侵袭能力的影响。方法:选取河北医科大学第四医院2017年6月—2018年6月收治的58例食管癌患者病理组织标本及配对癌旁组织。采用实时荧光定量PCR法检测癌及其相应癌旁组织中miR-1299的表达水平,并分析miR-1299表达与食管癌患者临床病理特征之间的关系。在食管癌TE1细胞中转入miR-1299模拟物(miR-1299-mimics)使miR-1299过表达。采用CCK-8法检测TE1细胞的增殖情况,Transwell小室法分别检测细胞的迁移和侵袭能力。最后,通过实时荧光定量PCR法和蛋白质印迹法检测miR-1299过表达的TE1细胞中侵袭转移标志物基质金属蛋白酶11(matrix metalloproteinase11,MMP11)和MMP16 mRNA和蛋白表达水平的变化。结果:miR-1299在食管癌组织中的表达水平明显低于相应的癌旁组织(P <0.001),而且miR-1299的表达水平与食管癌患者的临床分期具有相关性(P <0.05)。TE1细胞转入miR-1299-mimics后,miR-1299的表达水平明显升高(P <0.01),细胞的增殖能力无明显变化(P> 0.05),而细胞的迁移(P <0.001)和侵袭(P <0.01)能力明显降低,细胞侵袭转移相关标志物MMP11和MMP16 mRNA和蛋白的表达水平均明显下调(P值均<0.01)。结论:miR-1299在食管癌组织中表达下调,而且可能抑制食管癌细胞的迁移和侵袭能力。
Objective:To investigate the expression of microRNA-1299(miR-1299)in esophageal carcinoma and its effects on the migration and invasion of esophageal cancer TE1 cells.Methods:The pathological tissue specimens and their matched paracancerous tissues of58 patients with esophageal cancer were selected in the Fourth Hospital of Hebei Medical University from June 2017 to June 2018.The expression level of miR-1299 in cancer and corresponding adjacent tissues was detected by real-time fluorescent quantitative PCR.The relationship between miR-1299 expression and clinicopathological features was analyzed.After miR-1299-mimics were transfected into esophageal cancer TE1 cells for the overexpression of miR-1299,the proliferation of TE1 cells was detected by CCK-8 assay,and the migration and invasion abilities were detected by Transwell migration and invasion assays,respectively.Then the expressions of matrix metalloproteinase 11(MMP11)and MMP16 mRNAs and proteins in miR-1299 over-expressed TE1 cells were detected by realtime fluorescent quantitative PCR and Western blotting,respectively.Results:The expression of miR-1299 in esophageal cancer tissues was significantly lower than that in the adjacent tissues(P<0.001).The expression level of miR-1299 was associated with the clinical stage of esophageal cancer patients(P<0.05).After the transfection of miR-1299-mimics into TE1 cells,the expression level of miR-1299 was significantly increased(P<0.01).After the over-expression of miR-1299 in TE1 cells,the proliferation of TE1 cells did not change significantly(P>0.05),while the migration(P<0.001)and invasion(P<0.01)abilities decreased significantly,and the expressions of MMP11 and MMP16 mRNAs and proteins were obviously decreased(all P<0.01).Conclusion:The expression of miR-1299 is down-regulated in esophageal cancer tissues and may inhibit the migration and invasion of esophageal cancer cells.
作者
孟雪梅
刘思桦
常胜
张瑜
桑梅香
MENG Xuemei;LIU Sihua;CHANG Sheng;ZHANG Yu;SANG Meixiang(Dosing Center of Pharmacy;Research Center,Fourth Hospital of Hebei MedicalUniversity,Shijiazhuang050011,Hebei Province,China)
出处
《肿瘤》
CAS
CSCD
北大核心
2019年第8期623-631,共9页
Tumor
作者简介
Correspondence to:SANG Meixiang(桑梅香),E-mail:mxsang@hotmail.com
