摘要
用人体肺腺癌细胞系(LTEP-a-2)接种于裸小鼠皮下,成功地建立了一株可移植性瘤株模型。历时2年共传15代,共用62只裸小鼠,肿瘤总发生率为(54/62)87.1％。第7代以后肿瘤迅速生长,一般30天左右可长出500mm^3大小瘤体,仍保持肺腺癌的特征。
established in nude mice. Aliqouts (0.2ml) of 1 * 107LTEP-a-2 cells from the same batch were injected s.c. into 11 (1st group) and 5 (2nd group) nude mice. In the 1st group, 7/11 inoculated tumors showed growth (63.6%) , and in the 2nd group, 4/5 inoculated tumors showed growth (80%) . For establishment of human lung camcer cell line, tumor tissue from the 1st passage of growing tumor were transplanted s.c. into unde mice by method of small fragments or single cell suspension of tumor. During a period of two years, this transplanta'ble human lung cancer cell line has been transplanted in 62 nude mice with tumor growth incidence of 53/62(87.1%). After 7 passages, the tumor began to grow rapidly and attained large size in 4 weeks with maintenance of adenocarcinomatous structure.
出处
《中国医学科学院学报》
CAS
1987年第5期334-338,397-398,共7页
Acta Academiae Medicinae Sinicae
关键词
肺腺癌
裸小鼠
皮下移植
可移植性
lung adenocarcinoma nude mice subcutaneous inoculation transplantable tumor
