摘要
AIM:To investigate the pharmacological effect ofTong Xie-Yao Fang(TXYF)formula and its underlying mechanisms.METHODS:A neonatal maternal separation plus restraint stress(NMS+RS)model of diarrheapredominant irritable bowel syndrome was developed by subjecting male Sprague-Dawley rats to daily maternal separation from postnatal days 2 to 21 plus restraint stress from days 50 to 59.Rats were randomly divided into two groups(NMS+RS and TXYF formula),and rats with no handling or separation were used as normal controls.Starting from postnatal day 60,rats were administered TXYF formula(9.84 g/100 g body weight)orally twice daily for 14 consecutive days,while the normal and NMS+RS groups were given distilled water.The distinctions of movement index(MI,area under the curve of contraction intensity/min,mg/min)and contraction frequency(CF,number of contractions/min,times/min)of isolated colonic longitudinal smooth muscle strips(CLSMs)in the three groups before and after treatment were observed with a Power Lab system.Different inhibitors were applied,and then 10-4mol/L acetylcholine chloride(Ach)was added to CLSMs to induce muscle contraction.RESULTS:Before treatment,the MI of CLSMs in the NMS+RS and TXYF formula groups was similar and both higher than that in the normal group(545.49±73.66 mg/min vs 245.76±34.44 mg/min and551.09±54.29 mg/min vs 245.76±34.44 mg/min,P<0.01,respectively).After treatment,the MI in the TXYF formula group was lower than that in the NMS+RS group(261.39±38.59 mg/min vs 533.9±61.63 mg/min,P<0.01).In the same way,the CF of CLSMs in the NMS+RS and TXYF formula groups was similar and both higher than that in the normal group(3.42±0.25 times/min and 3.31±0.21 vs1.1±0.17 times/min,P<0.01)before treatment.After treatment,the CF in the TXYF formula group was lower than that in the NMS+RS group(1.42±0.87 times/min vs 3.11±0.82 times/min,P<0.01)and similar to that in the normal group(1.42±0.87 times/min vs 1.09±0.13 times/min).When8-(N,N-diethylamino)octyl 3,4,5-trimethoxybenzoate hydrochloride and 4-aminopyridine were added to the bath and equilibrated for 30 min,respectively,and10-4 mol/L Ach was added to CLSMs to induce muscle contraction,MI of the CLSMs in the TXYF formula group was lower than that in the normal group(666±36.32 mg/min vs 747.77±49.47 mg/min,and 686.53±39.17 mg/min vs 750.45±29.39 mg/min;P<0.01,respectively).The MI of CLSMs in the TXYF formula group was lower than that in the normal group after treatment with nifedipine(689.48±30.84 mg/min vs741.65±32.41 mg/min;P<0.05).CONCLUSION:TXYF formula inhibits colon contraction in rats.This may be related to activation of specific potassium channels and inhibition of extracellular calcium internal flow.
AIM:To investigate the pharmacological effect ofTong Xie-Yao Fang(TXYF)formula and its underlying mechanisms.METHODS:A neonatal maternal separation plus restraint stress(NMS+RS)model of diarrheapredominant irritable bowel syndrome was developed by subjecting male Sprague-Dawley rats to daily maternal separation from postnatal days 2 to 21 plus restraint stress from days 50 to 59.Rats were randomly divided into two groups(NMS+RS and TXYF formula),and rats with no handling or separation were used as normal controls.Starting from postnatal day 60,rats were administered TXYF formula(9.84 g/100 g body weight)orally twice daily for 14 consecutive days,while the normal and NMS+RS groups were given distilled water.The distinctions of movement index(MI,area under the curve of contraction intensity/min,mg/min)and contraction frequency(CF,number of contractions/min,times/min)of isolated colonic longitudinal smooth muscle strips(CLSMs)in the three groups before and after treatment were observed with a Power Lab system.Different inhibitors were applied,and then 10-4mol/L acetylcholine chloride(Ach)was added to CLSMs to induce muscle contraction.RESULTS:Before treatment,the MI of CLSMs in the NMS+RS and TXYF formula groups was similar and both higher than that in the normal group(545.49±73.66 mg/min vs 245.76±34.44 mg/min and551.09±54.29 mg/min vs 245.76±34.44 mg/min,P<0.01,respectively).After treatment,the MI in the TXYF formula group was lower than that in the NMS+RS group(261.39±38.59 mg/min vs 533.9±61.63 mg/min,P<0.01).In the same way,the CF of CLSMs in the NMS+RS and TXYF formula groups was similar and both higher than that in the normal group(3.42±0.25 times/min and 3.31±0.21 vs1.1±0.17 times/min,P<0.01)before treatment.After treatment,the CF in the TXYF formula group was lower than that in the NMS+RS group(1.42±0.87 times/min vs 3.11±0.82 times/min,P<0.01)and similar to that in the normal group(1.42±0.87 times/min vs 1.09±0.13 times/min).When8-(N,N-diethylamino)octyl 3,4,5-trimethoxybenzoate hydrochloride and 4-aminopyridine were added to the bath and equilibrated for 30 min,respectively,and10-4 mol/L Ach was added to CLSMs to induce muscle contraction,MI of the CLSMs in the TXYF formula group was lower than that in the normal group(666±36.32 mg/min vs 747.77±49.47 mg/min,and 686.53±39.17 mg/min vs 750.45±29.39 mg/min;P<0.01,respectively).The MI of CLSMs in the TXYF formula group was lower than that in the normal group after treatment with nifedipine(689.48±30.84 mg/min vs741.65±32.41 mg/min;P<0.05).CONCLUSION:TXYF formula inhibits colon contraction in rats.This may be related to activation of specific potassium channels and inhibition of extracellular calcium internal flow.
基金
Supported by National Nature Science Foundation of China,No.81473644
the Clinical Medicine Development Project of Beijing Municipal Administration of Hospitals,No.ZY201411
the Project of Health Technical Personnel of the Beijing Health System,No.2011-2-13
